• Preventive Nutrition and Food Science
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• 제22권2호
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• pp.138-143
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• 2017

The antifungal activity of isolated lactic acid bacteria (LAB) from a locally fermented cereal, "Kunu", was tested against toxigenic Aspergillus flavus. The liquid refreshment, "Kunu", was prepared under hygienic condition using millet, sorghum, and the combination of the two grains. The antifungal potential of isolated LAB against toxigenic A. flavus was carried out using both in vitro and in vivo antifungal assays. The LAB count from prepared "Kunu" ranged from $2.80{\times}10^4CFU/mL$ to $4.10{\times}10^4CFU/mL$ and Lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus fermentum, Pediococcus acidilactici, and Leuconostoc mesenteroides were the isolated bacteria. Inhibitory zones exhibited by LAB against toxigenic A. flavus ranged from 5.0 mm to 20.0 mm. The albino mice infected with toxigenic A. flavus showed sluggishness, decrease in body weight, distortion of hair, and presence of blood in their stool, while those treated with LAB after infection were recovered and active like those in control groups. Except for the white blood cell that was increased in the infected mice as $6.73mm^3$, the packed cell volume, hemoglobin, and red blood cell in infected animals were significantly reduced (P<0.05) to 29.28%, 10.06%, and 4.28%, respectively, when compared to the treated mice with LAB and control groups. The antifungal activity of LAB against toxigenic A. flavus can be attributed to the antimicrobial metabolites. These metabolites can be extracted and used as biopreservatives in food products to substitute the use of chemical preservatives that is not appealing to consumers due to several side effects.

• 대한추나의학회지
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• 제3권1호
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• pp.1-14
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• 2002

Objectives : To investigate the effects of Saengmaeksan-with-Ogapy on the recovery of fatigue induced by post-swimming exercise. Methods : The lactic acid, creatine phosphokinase(CPK), lactate dehydrogenase(LDH) and glucose were measured in blood. Results: The result were as follows; 1. The lactic acid levels in Saengmaeksan and Saengmaeksan-with-Ogapy group were immediately decreased at 30 minutes after exercise and statistically showed the significance different from the control group (P<0.005). 2. The CPK activities in Saengmaeksan and Saengmaeksan-with-Ogapy group were immediately decreased after exercise and statistically showed the significant difference compared with the control group (P<0.05). 3. The LDH activities in Saengmaeksan and Saengmaeksan-with-Ogapy group did not show the significant difference compared with the control group. 4. The glucose levels in Saengmaeksan and Saengmaeksan-with-Ogapy group did not statistically show the significant difference compared with the control group. Conclusions So it could be concluded that saengmaeksan and Saengmaeksan-with-Ogapy can recover the fatigue of after exercise.

• 국제물리치료학회지
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• 제1권1호
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• pp.36-44
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• 2010

This study has been conducted on the subjects of eight undergraduate students with abdominal obesity and eight undergraduate students with normal weight to find out correlation between substantial fatigue and Ratings of perceived exertion through analysis of their blood components when they took exercises to strengthen their abdominal muscular power. Comparatively analyzing HDL-C, LDL-C and lactic acid before and after they took sit-up at level of RPE 19-20, no statistically significant differences in HDL, LDL, lactic acid measures were observed between groups, but a significant difference in RPE was noted between groups(p<0.05) Our findings suggest that control group showed no significant difference in increase of fatigue material whereas the obese group showed a lower frequency of sit-up, though both groups took the same abdominal exercise. Additionally, the lower frequency of sit-up in obese group results from relatively higher Ratings of perceived exertion rather than increase of substantial fatigue material. This study invites future research that examines the effect of a comprehensive obesity exercise program combined with dietetic on ratings of perceived exertion in individuals with obesity.

• 대한임상독성학회지
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• 제2권2호
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• pp.96-100
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• 2004

Purpose: This study was to investigate the effects of ethanol in ingested patients by analyzing data from a single institution's registry, Methods: We conducted a prospective study of 50 patients who has ingested drugs with/without ethanol came to emergency department from January 2004 to May 2004. Only patients over 18 years of age were included. Clinical characteristics, general and specific treatment, laboratory finding, complication, and clinical outcomes were obtained from protocol. Patients were divided into two groups: drug ingested with alcohol (ethanol group, n=18), and ingested without alcohol (non-ethanol group, n=32). Results: The age, the amout of ingestion, the time to treatment, the systolic blood pressure, the diastolic blood pressure and the shock duration were not different between two groups. The AST level with the ethanol group was higher than with the non-ethanol group ($230.94\pm518.88$ U/L vs $43.22\pm63.39$ U/L, p=0.002). The ALT level with the ethanol group was higher than with the non-ethanol group ($97.06\pm152.98$ U/L vs $32.75\pm43.10$ U/L, p=0.001). The lactic acid level with the ethanol group was higher than with the non-ethanol group ($7.40\pm6.33$ mmol/L vs $3.77\pm3.10$ mmol/L, p=0.001). The hospital stay duration and the admission rate were not different between two groups. Conlusions: The ethanol increased the levels of serum AST, ALT and lactic acid in intoxicated patients. But the ethanol dose not increase admission rate and duration of admission stay in intoxicated patients.

• Journal of Chest Surgery
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• 제10권2호
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• pp.250-267
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• 1977

Open heart surgery has been performed on 20 patients, using hemodilution principle under the moderate hypothermia from Dec. 1975 through Aug. 1977 at the Department of Thoracic and Cardiovascular Surgery, Kyungpook National University School of Medicine. All these patients, body surface area ranged from 0.53 to 1.67$M^2$, were divided into two groups as a child group [below 15 yrs] and a adult group[above 15 yrs]. The oxygenator were primed with fresh ACD blood, 5 per cent dextrose, Hartmann`s solution, 15 per cent mannitol, sodium bicarbonate, dexamethasone and antibiotics. The average flow rate was 2.0 L/$M^2$/min. in child group and 2.3L/$M^2$/min. in adult group. The degree of hemodilution in child and adult group was 30.7% and 29.3% respectively. The minimal value of rectal temperature was $30.84{\pm}0.7^{\circ}C.$, in child group and $30.0{\pm}1.5^{\circ}C.$, in adult group. We studied the changes of hemodynamic status, blood components, electrolyte, acid-base status. blood lactic acid and urine output during and after cardiopulmonary bypass.

• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.794-797
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• 1999

Lactobacillus acidophilus is known to have an inhibitory activity on growth of Helicobacter pylori and this activity has been attributed to lactic acid and antibacterial agents produced by Lactobacillus. Since every Lactobacilli produces lactic acid, an another factor must exist for L. acidophilus to inhibit H. pylori growth. In this work, the inhibitory activity of L. acidophilus on H. pylori adherence was studied. An immunoabsorbent assay using TLC plate was developed and used for screening the inhibitory activity of various Lactobacilli on H. pylori adherence. Glycolipid, the attachment site for H. pylori, was isolated from blood type O red blood cells and spotted on a TLC plate. The H. pylori adherence increased linearly with increasing amounts of glycolipid spotted on the TLC plate. Various L. acidophilus strains, but not L. casei, appeared to inhibit H. pylori adherence to glycolipid, and the adherence decreased linearly as the concentration of the Lactobacillus increased. The results show that the inhibitory activity of L. acidophilus on H. pylori adherence is an another factor for L. acidophilus to inhibit H. pylori growth.

• Journal of Pharmaceutical Investigation
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• 제38권1호
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• pp.63-72
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• 2008

Meloxicam-loaded microspheres were prepared with poly(D,L-lactic acid)(PLA) by a solvent-emulsion evaporation method. The morphology, particle size, drug loading capacity, drug entrapment efficiency (EE) and release patterns of drug were investigated in vitro. Various batches of micro spheres with different size and drug content were obtained by changing the ratio of meloxicam to $PLA^{\circ}{\AE}s$ with different molecular weight, PLA concentration in the dispersed phase and stirring rate. Meloxicam crystals on microsphere surface, which were released rapidly and could act as a loading dose, were observed with increasing drug content. The release rate was increased with increase in drug contents and decrease in the molecular weight of PLA. Microspheres prepared with smaller molecular weight produced faster drug release rate. The release rate of meloxicam for long-acting injectable delivery system in vitro, which would aid in predicting in vivo release profile, could be controlled by properly optimizing various factors affecting characteristics of microspheres. Blood concentration-time profile of meloxicam after intramuscular injection of meloxicam-loaded microspheres in rabbits showed possibility of long term application of this system in clinical settings.

• 대한통합의학회지
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• 제8권4호
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• pp.133-142
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• 2020

Purpose : The purpose of this study was to investigate the effective recovery method of exercise-induced fatigue and muscle pain by comparing the effect of the use of illite hot mat product and general hot mat product on the recovery of muscle pain induced by high intensity exercise. Methods : To measure and analyze the changes in lactic acid, CRP, and ACR according to the high-intensity circuit training program, this study was conducted for the healthy adult men and women, who exercise st the K-region sports center. A total of 45 subjects were studied in 15 groups of 15 patients who received an illite hot-water mat recovery group (A group), 15 general hot-water mat recovery group (B group), and 15 control group (C group). The circuit training exercise program was conducted as a one-time exercise, and each exercise time consisted of 30 minutes of warm-up exercise, 5 minutes of main exercise, 20 minutes of clean-up exercise, and 5 minutes of strength exercise. The intensity setting was high intensity of subjective exercise intensity It carried out by setting to (14-16RPE). Results : Changes in Lactic Acid Concentration There was a significant difference in the lactic acid concentrations between the groups after the high intensity circuit training program (p <.05). The illite rest group (A) decreased 7.71 mmol / L and the control group decreased 4.03 mmol / L. Significantly decreased (p <.05). Changes in ACR Concentration. There was a significant difference in the ACR concentrations Significant differences were found in CRP and ACR during the recovery period after exercise. (p <.05), the elite rest group (A) decreased 2.47 mg / mmol, and the control group increased 1.63 mg / mmol. There was a significant difference (p <.05). Conclusion: The static rest on a heated mat after high-intensity exercise has an effect on changes in blood lactate and ACR levels.

• 한국미생물·생명공학회지
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• 제51권1호
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• pp.10-17
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• 2023

Dairy products are extensively used as carriers of probiotic strains that have potential health benefits. Assessment of the viability of probiotic strains during manufacturing is important to ensure that products meet recommended levels. Hence, the method for accurately quantifying lactic acid bacteria (LAB) in probiotic or dairy products is required. The present study aims to examine the performance of de-Man Rogosa Sharpe (MRS), plate count agar with bromocresol purple (PCA with BCP), and glucose blood liver (BL) agars recommended in the Korea Food Code guidelines for counting LAB. Analysis of the performance of culture media containing 19 lactic acid bacterial species commonly encountered in probiotic and dairy products showed no statistically significant difference between 18 reference strains and three culture media (p > 0.01). Furthermore, the suitability of three culture media was verified for the quantitative assessment of LAB in 25 probiotic and dairy products. The number of LAB in three culture media was determined to be more than 10⁷ colony-forming unit (CFU)/ml for fermented milk products and 10⁸ CFU/ml for condensed fermented milk and probiotic products, indicating that they all satisfied the Korea Food Code guidelines. Moreover, there was no statistically significant difference in the amount of LAB counted in all three culture media, suggesting that they can be used to isolate or enumerate LAB in commercial products. Finally, three culture media will be useful for isolating and enumerating LAB from fermented foods as well as gut microflora.

• Journal of Nutrition and Health
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• 제30권10호
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• pp.1188-1194
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• 1997

The aim of this was to investigate whether the regular consumption of kimchi influences the iron status (RBC , Hb, Ht, MCH, MCV, MCHC, transferrin , serum iron, and ferritin) in volunteers. Healthy male adults(n=12) took part in the study subdivided into the control Ⅰ-phase(for 2weeks), kimchi-phase (for 4 weeks), and control Ⅱ-phase(for 2 weeks). In addition to their normal diet, participant consumed 300g of lactic acid fermented Chinese cabbage kimchi daily for four weeks. In the control Ⅰ and control Ⅱ phases, the participants kept up their normal diets without consuming any fermented foods. Dietary intakes were recorded for 3 consecutive days in each phase, with the aid of household measures. Every two weeks. blood specimens were analysed. Significant differences(p<0.05) between the phases were found in MCHC, and transferrin in blood were not significantly changed during kimchi consumption. However, serum iron and ferritin levels were significantly increased(p<0.05) during kimchi consumption, achieving the highest levels in the fourth week of the kimchi components(ascrobic acid, sulfer compound, organic acid, capsaicin, gingerol , allicin). Because of lacticacid fermented kimchi's potential to prevent anemia , the consumption of this food can be recommended.