• Journal of Veterinary Clinics
• /
• v.13 no.2
• /
• pp.127-129
• /
• 1996

The study was conducted to determine normal blood and serum chemical values on deer. Normal blood and serum chemical values were studied in 8 healthy deers bred at Dalseung Park in Taegu. The values were analyzed with a blood chemical analyzer and calculated by Chawen's statistical analysis. The blood values of RBC, WBC and PCV were $9.73{\pm}1.19{\times} 10^{6}/{\mu}l, 3.37{\pm}0.88{\times}10^{3}/{\mu}l, 47.25{\pm}4.65 % respectively. The serum chemical value were: GOT; 83.0{\pm}39.4 IU/L, GPT; 50.12{\pm}25.83 IU/L, BUN; 36.63{\pm}8.70 mg/dl. CPK ; 1,296{\pm}495 IU/L, ALP; 238{\pm}54 IU/L, glucose; 143{\pm}52 mg/dl, total protein; 6.95{\pm}0.61 g/dl, amylase ; 116.0{\pm}34.5 IU/L,$ respectively. These values will be clinically helpful to diagnose the disease of deer.

• Journal of Biomedical Engineering Research
• /
• v.11 no.1
• /
• pp.97-104
• /
• 1990

Microvel double velour graft impregnated with a biodegradable algin was studied as a new vascular graft. It is blood tight but still retains high porosity. Thls graft does not need to be preslotted with blood before implantation and has good tissue ingrowth and biological healing properties. The algin impregnated vascular graft was investigated by "in vitro" tests in this study. It was characterl zed by ESCA analysis, SEM observation, and measurements of water permeability, algin coating weight, mechanical properties and whole blood clotting time. The water permeability of the graft was reduced more than 99% and the whole blood clotting time was fast more than three times by the algin impregnation treatment. "In vivo" performance examinations of the algin impregnated graft are on progress.aft are on progress.

• Journal of Ecology and Environment
• /
• v.38 no.3
• /
• pp.307-318
• /
• 2015

The objectives of this study were to identify multi-level stressors from blood biomarkers to community-level bioindicators and diagnose the stream ecosystem health in polluted streams. Blood chemistry such as total protein ($T_{Pro}$), blood urea nitrogen ($B_{UN}$), total cholesterol ($T_{Cho}$) and $A_{lb}$umin ($A_{lb}$) were analyzed from sentinel fish tissues; the functions of kidney, gill and liver were significantly decreased in the impacted zone ($I_z$), compared to the control zone ($C_z$). Histopathological analysis showed that fish liver tissues were normal in the $C_z$. Fish liver tissues in the $I_z$, however, showed large cell necrosis and degeneration and also had moderate lobular inflammation and inflammatory cell infiltration of lymphocytic histocytes. Species biotic index (SBI) at species level and stream health assessment (SHA) at community level indicated that chemical impacts were evident in the $I_z$ (ecological health; poor - very poor), and this was matched with the blood tissue analysis and histopathological analysis. The impairments of the streams were supported by water chemistry analysis (nitrogen, phosphorus). Tolerance guild analysis and trophic guild analysis of fish were showed significant differences (P < 0.01) between $C_z$ and $I_z$. Overall, multiple parameter analysis from biomarker level (blood tissues) to bioindicator level (community health) showed significantly greater impacts in the $I_z$ than $C_z$. This approach may be effective as a monitoring tool in identifying the multilateral and forthcoming problems related to chemical pollution and habitat degradation of stream ecosystems.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.10
• /
• pp.3103-3106
• /
• 2014

• Journal of Veterinary Clinics
• /
• v.15 no.1
• /
• pp.219-221
• /
• 1998

This report was conducted to determine the changes of blood and serum chemical values on dogs infected with Babesia gibsoni. Blood and serum were obtained and analyzed with a blood chemical analyzer and calculated by Chauvent's statistical analysis. The mean values and SD were as follows, $AST 33{\pm} 23 IU/L, ALT 22{\pm}15 IU/L, BUN 18$\pm $8 mg/dl, creatinine 1.6{\pm}0.8 mg/dl, CPK 564{\pm}214 IU/L, LDH 208{\pm} 78 IU/L, ALP 52{\pm} 8 IU/L, glucose 89{\pm}38 mg/dl, RBC 3.04{\pm}0.77{\times}10^{6}/{\mu}l, hemoglobin 6.1{\pm} 1.3 g/dl, PCV 18.0{\pm }4.3%, $respectively.

• Korean Chemical Engineering Research
• /
• v.58 no.1
• /
• pp.106-112
• /
• 2020

Analyzing vitamin D levels is important for monitoring health conditions because vitamin D deficiency is associated with various diseases such as rickets, osteomalacia, cardiovascular disorders and some cancers. However, vitamin D concentration in the blood is very low with optimal level of 75 nmol/L, making quantitative analysis difficult. The objective of this study was to develop a highly sensitive analysis method for vitamin D using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS). 25-hydroxyvitamin D (25(OH)D), which has been used as an indicator of vitamin D metabolites in human biofluids was chemically derivatized using a secosteroid signal enhancing tag (SecoSET) with powerful dienophile and permanent positive charge. The SecoSET-derivatized 25(OH)D provided good linearity (R² > 0.99) and sensitivity (limit of quantitation: 11.3 fmol). Chemical derivatization of deuterated 25-hydroxyvitamin D₃ (d₆-25(OH)D3) with SecoSET enabled absolute quantitative analysis using MALDI-MS. The highly sensitive method could be successfully applied into monitoring of quantitative changes of bioactive vitamin D metabolites after treatment with ketoconazole to inhibit 1α-hydroxylase reaction related to vitamin D metabolism in human breast cancer cells. Taken together, we developed a MALDI-MS-based platform that could quantitatively analyze vitamin D metabolites from cell products, blood and other biofluids. This platform may be applied to monitor various diseases associated with vitamin D deficiency such as rickets, osteomalacia and breast cancer.

• Biomedical Science Letters
• /
• v.18 no.4
• /
• pp.406-412
• /
• 2012

The polymorphism (insertion, I or deletion, D) of angiotensin converting enzyme (ACE) gene is designated as the presence of a 287 bp Alu repeat. The D/D homozygote carrier is associated with high ACE activity, and this high activity has been implicated with hypertension, coronary artery disease, or diabetic nephropathy. We studied the clinical candidate marker in ACE gene polymorphism using chemical and hematological analysis. The subjects are divided into normotensive and hypertensive groups and ACE genotype in the group was confirmed by PCR method. Chemical analysis was preceded with Hitachi7060, and hematological analysis was performed using Mythic 22. In 116 targeted people, 17 (38.64%) of 44 I/I genotype group are hypertension, 15 (34.09%) in 44 with D/I, but, D/D type in the 28 cases is 15 patients (53.57%) in hypertension. In hypertension group, biochemical analysis (triglyceride, and alkaline phosphatase) and hematological analysis (white blood cell, platelet) are showed high value in D/D genotype of ACE gene. The relationship between hypertension and ACE genotype is the same results as previously reported and we thought that the high laboratory value of white blood cell, platelet, triglycerides, and alkaline phosphatase are also indicator of hypertension in D/D type of ACE.

• Journal of Veterinary Clinics
• /
• v.18 no.4
• /
• pp.478-481
• /
• 2001

A Patient (Korean native cow, 4 years old, 300 kg, female), with chief complaint of anorexia for long period was admitted to the Chungnam national University Veterinary Medical Teaching Hospital. At necropsy findings, hard masses of necrotic fat were located in the omentum, rectum, kidney, colon, heart and thoracic cavity, respectively. The yellowish changes also were observed in liver, spleen and lung, respectively. In histopathological findings, fatty accumulation was observed by microscopic observation in each organ of fatty masses. Hamatological analysis revealed the low levels of Hb, PCV and neutrophil. Blood chemical analysis indicated the high concentration of Cholestelol and Triglyceride. That patient was diagnosed as typical case of lipomatosis by clinical, blood, blood chemical and pathological examinations.

• Korean Chemical Engineering Research
• /
• v.49 no.2
• /
• pp.238-243
• /
• 2011

In this study, we attempted a structural analysis in order to design a balloon type extracorporeal membrane oxygenator that can induce blood flow without using blood pumps for the purpose of complementing the weakness in the existing extracorporeal membrane oxygenator. To analyze the flow characteristic of the blood flow within the virtual model of extracorporeal membrane oxygenator, computational fluid dynamics(CFD) modeling method was used. The operating principle of this system is to make the surface of the extracorporeal membrane oxygenator keep contracting and dilating regularly by applying pressure load using a balloon, and the 'ime Function Value'that changes according to the time was applied by calculating a half cycle of sine waveform and a cycle of sine.waveform Under the assumption that the uni-directional blood flow could be induced if the balloon type extracorporeal membrane oxygenator was designed as per the method described above, we conducted a structural analysis accordingly. We measured and analyzed the velocity and pressure of blood flow at both inlet and outlet of the extracorporeal membrane oxygenator through CFD simulation. As a result of the modeling, it was confirmed that there was a flow in accord with the direction of the blood by the contraction/dilation. With CFD simulation, the characteristics of blood flow can be predicted in advance, so it is judged that this will be able to provide the most optimized design in producing an extracorporeal membrane oxygenator.

• YAKHAK HOEJI
• /
• v.52 no.2
• /
• pp.137-146
• /
• 2008

Even though driving under the influence of drug (DUID) is a worldwide problem, we, Korea has no regulation system yet except for alcohol, and there are little cases reported related to DUID. In order to investigate the type of abused drugs for drivers in Korea, we tried to analyze controlled and non-controlled drugs in alcohol-positive blood samples. 275 whole bloods, which were positive for alcohol on the roadside test, were collected from the police for two months ($Nov.{\sim}Dec.$ 2006). The analytical strategy was constituted of three steps: First, alcohol in blood samples were confirmed and quantified by gas chromatography. Second, controlled drugs were screened by $Evidence_{investigator}\;^{TM}$ (Randox, U.K.) as preliminary test. It was based on immunoassay by biochip array analyzer. Nine groups of drug abuse were screened: amphetamines, methamphetamines, cannabis, cocaine, opiates, barbiturates, methadone, benzodiazepines I (oxazepam) & II (lorazepam). Finally, confirmation of these drugs was performed by GC-MS. Blood samples were extracted by solid-phase extraction by $RapidTrace^{TM}$ (Zymark, U.S.A.). After trimethylsilyl (TMS) derivatization, eluates were analyzed to GC-MS. Total 49 drugs were investigated in this study including controlled drugs, antidepressants, 1st generation antihistamines, dextromethorphan, nalbuphine, ketamine, etc. For rapid detection, we developed the automated identification system. It was made up a new software, "DrugMan", modified Chemstation data analysis menu and newly developed macro modules. A series of peak selection, identification and reporting of the results were performed automatically by this system. Concentrations of alcohol in 275 blood samples were ranged from 0.011 to 0.249% (average, 0.119%). Among 149 blood samples, just six samples (4.0%) were showed positive results to the immunoassay: one methamphetamine and five benzodiazepines group I. By GC-MS confirmation, only benzodiazepines were detected and methamphetamine was not detected from immunoassay positive blood sample. Besides these drugs, 5 chlorpheniramines, dextromethorphan, diazepam, doxylamine, ibuprofen, lidocaine and topiramate were also detected in whole bloods by GC-MS. Conclusively, the frequency of drug abuse for Korean drivers was relatively low. There was none case which illegal drug was detected. However these results were limited to alcohol positive blood samples, so it is necessary to analyze more samples including alcohol negative blood.