• 한국육종학회지
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• 제40권3호
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• pp.215-222
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• 2008

Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.

• 한국전산구조공학회논문집
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• 제30권5호
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• pp.427-434
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• 2017

최근 테러에 대한 위험성의 증가로 대중들의 폭발 피해에 대한 인식이 증가하였다. 우리나라에 방폭 설계에 대한 기준이 미흡하며, 현재 적용하고 있는 방폭 설계도 정적해석으로 건물의 안정성 및 경제성을 위해 방폭 설계를 개발해야 하는 상황이다. 또한 지진 발생 증가로 내진 설계 의무화가 확대된 가운데 방폭 설계를 하지 않고 내진 설계를 적용한 부재의 방폭성능을 판단을 연구한다. 현재 보편적인 폭발 하중의 해석 방법은 UFC 3-340-02 매뉴얼을 참고하는 것이다. UFC 3-340-02 매뉴얼을 통한 폭발 하중의 특성을 적용하고 KBC 2016의 내진 상세를 적용한 보를 등가 단자유도 시스템으로 변환하여 폭발저항 성능을 연구하였다. 보통, 중간, 특수 모멘트 골조의 연성 능력에 대한 최대 처짐을 고려하여 폭발물의 이격 거리를 통해 평가하여 내진 상세 적용 시 폭발 저항 성능이 향상된다는 것을 입증하였다.

• The Plant Pathology Journal
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• 제31권1호
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• pp.12-24
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• 2015

Rice Blast is the most devastating disease causing major yield losses in every year worldwide. It had been proved that using resistant rice varieties would be the most effective way to control this disease. Molecular screening and genetic diversities of major rice blast resistance genes were determined in 192 rice germplasm accessions using simple sequence repeat (SSR) markers. The genetic frequencies of the 10 major rice blast resistance genes varied from 19.79% to 54.69%. Seven accessions IC337593, IC346002, IC346004, IC346813, IC356117, IC356422 and IC383441 had maximum eight blast resistance gene, while FR13B, Hourakani, Kala Rata 1-24, Lemont, Brown Gora, IR87756-20-2-2-3, IC282418, IC356419, PKSLGR-1 and PKSLGR-39 had seven blast resistance genes. Twenty accessions possessed six genes, 36 accessions had five genes, 41 accessions had four genes, 38 accessions had three genes, 26 accessions had two genes, 13 accessions had single R gene and only one accession IC438644 does not possess any one blast resistant gene. Out of 192 accessions only 17 accessions harboured 7 to 8 blast resistance genes.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.112-112
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• 2017

Rice blast caused by Magnaporthe oryzae is one of the most serious diseases that affect the quantity and quality of rice production. The use of resistant rice varieties would be the most effective way to control the rice blast. However R gene incorporation into the rice variety takes time and pathogen could overcome the R gene effects after for a while. For monitoring the rice blast resistance gene distribution in Korean varieties, the four major blast resistance genes against M. oryzae were screened in a number of Korean rice varieties using molecular markers. Of the 120 rice varieties tested, 40 were found to contain the Pi-5 gene, 25 for the Pi-9 gene, 79 for Pi-b and 40 for the Pi-ta gene. None of these rice varieties includes tested 4 R genes. 3 R genes combination, Pi-5/Pi-9/Pi-b, Pi-5, Pi-9.Pi-ta, or Pi-9/Pi-b/Pi-ta were found in 12 varieties, the rice blast disease severity were showed as resistant in the rice verities containing Pi-9/Pi-b/Pi-ta R genes combination, respectively. Also pathogenic diversity of M. oryzae isolates collected in the rice field from 2004 to 2015 in rice field in Korea were analyzed using rice blast monogenic lines, each harboring a single blast resistance gene. Compatibility of blast isolates against rice blast monogenic lines carrying the resistance genes Pi5, Pi9, Pib, and Piz showed dynamic changes by year. It indicates that pathogen has high evolutionary potential adapted host resistances to increase fitness and would lead to rice blast resistance bred into the cultivar becoming ineffective eventually.

• 한국자원식물학회지
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• 제27권6호
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• pp.687-700
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• 2014

Rice blast (Magnaporthe oryza B.) is one of the most important diseases in rice that causing great yield losses every year around the world. It is important to screen valuable genetic resources for improving blast resistance. This study was conducted to identify the blast resistance in 279 Korean rice landraces using blast nursery tests and isolate inoculum screening. The results showed that 11 landrace accessions found to be resistant to rice blast in blast nursery and inoculation screening tests and the degree of lesions in most accessions showed that they were susceptible to reactions. In order to find the distribution of blast resistant genes, a molecular survey was conducted to identify the presence of major blast resistance (R) gene in 279 Korean landraces. The results revealed that their frequency distribution was Pik-m (36.2%), Piz (25.4%), Pit (13.6%), and Pik (10%). Besides, the frequency distribution of Piz-t, Pii, Pik-m/Pik-p, Pi-39(t), Pib, Pi-d(t)2, Pita/Pita-2 and Pi-ta genes were identified as less than 10%. The results did not consist with the reactions against blast diseases between genotypes and phenotypic part of the nursery tests and isolate inoculation. For concluding these results, we used genome-wide SSR markers that have closely been located with resistance genes. The PCoA analysis showed that the landrace accessions formed largely two distinct groups according to their degree of blast resistance. By comparing genetic diversities using polymorphic information contents (PIC) value among the resistant, total and susceptible landraces, we found that PIC values decreased in four SSR markers and increased in six markers in the resistant accessions, which showed contrary to total and susceptible groups. These regions might be linked to resistance alleles. In this study, we evaluated the degree of blast resistance and the information about the distribution of rice blast resistant genes in Korean rice landraces. This study might be the basis for association analysis of blast resistance in rice.

• 콘크리트학회논문집
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• 제12권4호
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• pp.41-48
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• 2000

This study is to investigate for the frost resistance of high-strength concrete using finely ground granulated blast-furnace slag with experimental parameters, such as water/binder ratio, replacement proportion of granulated blast-furnace slag, air content and methods of curing. The high-strength concrete using granulated blast-furnace slag is effective to resist frost and decrease scaling. The more increasable replacement proportion of granulated blast-furnace slag is, the better the effect is. The high-strength concrete using granulated blast-furnace slag needs hydrating adequately to prevent deterioration by drying in the early curing period. The micro structure of high-strength concrete, increased to the pore number with diameter of 0.03~0.1mm, is changed by using granulated blast-furnace slag, but is presented differently according to water/binder ration and replacement proportion of granulated blast-furnace slag.

• 한국자원식물학회지
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• 제29권6호
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• pp.658-669
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• 2016

Rice blast, caused by a fungus Magnaporthe oryzae, is one of the most devastating diseases of rice worldwide. Analyzing the valuable genetic resources is important in making progress towards blast resistance. Molecular screening of major rice blast resistance (R) genes was determined in 2,509 accessions of rice germplasm from different geographic regions of Asia and Europe using PCR based markers which showed linkage to twelve major blast R genes, Pik-p, Pi39, Pit, Pik-m, Pi-d(t)2, Pii, Pib, Pik, Pita, Pita/Pita-2, Pi5, and Piz-t. Out of 2,509 accessions, only two accessions had maximum nine blast resistance genes followed by eighteen accessions each with eight R genes. The polygenic combination of three genes was possessed by maximum number of accessions (824), while among others 48 accessions possessed seven genes, 119 accessions had six genes, 267 accessions had five genes, 487 accessions had four genes, 646 accessions had two genes, and 98 accessions had single R gene. The Pik-p gene appeared to be omnipresent and was detected in all germplasm. Furthermore, principal component analysis (PCA) indicated that Pita, Pita/Pita-2, Pi-d(t)2, Pib and Pit were the major genes responsible for resistance in the germplasm. The present investigation revealed that a set of 68 elite germplasm accessions would have a competitive edge over the current resistance donors being utilized in the breeding programs. Overall, these results might be useful to identify and incorporate the resistance genes from germplasm into elite cultivars through marker assisted selection in rice breeding.

• 콘크리트학회논문집
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• 제12권2호
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• pp.43-51
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• 2000

This study is to investigate for the frost resistance of high-flowing concrete using finely ground granulated blast-furnace slag with experimental parameters, such as type of binder, type of superplasticizer and method of curing. The resistance to freezing and thawing of high-flowing concrete by type of binder and superplasticizer is presented differently. Though the frost resistance of high-flowing concrete is satisfactory under standard condition, it is required that high-flowing concrete has entrained air like plain concrete. Because the critical spacing factor, being capacity of frost resistance, of high-flowing concrete is longer that of plain concrete, the frost resistance of high-flowing concrete, using finely ground granulated furnace blast slag, is superior to that of plain concrete.

• International Journal of Concrete Structures and Materials
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• 제10권sup3호
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• pp.87-96
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• 2016

This numerical study investigated the effects of different reinforcing details in beam-column joints on the blast resistance of the joints. Due to increasing manmade and/or natural high rate accidents such as impacts and blasts, the resistance of critical civil and military infrastructure or buildings should be sufficiently obtained under those high rate catastrophic loads. The beam-column joint in buildings is one of critical parts influencing on the resistance of those buildings under extreme events such as earthquakes, impacts and blasts. Thus, the details of reinforcements in the joints should be well designed for enhancing the resistance of the joints under the events. Parameters numerically investigated in this study include diagonal, flexural, and shear reinforcing steel bars. The failure mechanism of the joints could be controlled by the level of tensile stress of reinforcing steel bars. Among various reinforcing details in the joints, diagonal reinforcement in the joints was found to be most effective for enhancing the resistance under blast loads. In addition, shear reinforcements also produced favourable effects on the blast resistance of beam-column joints.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 1999년도 학회창립 10주년 기념 1999년도 가을 학술발표회 논문집
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• pp.671-674
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• 1999

With increasing content of granulated blase furnace slag in cement, the content of capillary pores in the mortar decreases and later age strength of the concrete. Therefore, this provides greater reserves study is carried out to estimate frost resistance of high-strength concrete specimens with water cement ratios using blast-furnace slag. 1. Blast furnace concrete is comparatively more good frost resistance than normal concrete. 2. As the blast furnace slag increases, the quantity of pores with a radius of more than 30nanometer decreses.