• 한국어병학회지
• /
• 제13권2호
• /
• pp.143-146
• /
• 2000

본 연구에서는 bronopol과 bithionol을 이용하여 양식 넙치에 기생하는 Ichthyobodo sp.의 치료효과를 조사하였으며, 그 효과를 formalin과 비교하였다. formalin 200 ppm 혹은 bithionol 100 ppm에서 1 시간동안 약욕한 결과 모든 실험어에서 100% 치료효과를 나타내었으며, 이보다 더 낮은 농도(formalin 50 ppm, 100 ppm 및 bithionol 25 ppm, 50 ppm)에서의 약욕에 의해서도 Ichthyobodo sp.의 감염강도를 유의적으로 감소시켰다. 그러나 bronopol을 이용한 약욕 및 bithionol의 경구투여는 대조구에 비해 유의적인 치료효과를 나타내지 않았다.

• 대한수의학회지
• /
• 제6권1호
• /
• pp.45-52
• /
• 1966

Hexachlorophene, Bithionol and Dithiazanine iodide were used to examine the vermicidal effect on Fasciola hepatica in vitro. A kymographic study was performed to investigate the motility of Fasciola hepatica under the influence of these drugs. Following conclusions were made: 1) With 10,000 : 1 solution; exhibited the vermicidal effect on Fasciola hepatica, Hexachlorophene in 5 minutes, Bithionol in 10 minutes, and Dithiazanine, iodide in 40 minutes, respectively. 2) With 100,000 : 1 solution; exhibited the vermicidal effect on Fasciola hepatica, Hexachlorophene in 20 minutes and Bithionol in 30minutes. In the case of Dithiazanine iodide the stimulation upon the fluke continued for 120 minutes. 3) With 1,000,000 : 1 solution; exhibited the vermicidal effect on Fasciola hepatica, Hexachlorophene in 50 minutes and Bithionol in 80 minutes. In the case of Dithiazanine iodide the stimulation upon the fluke continued for 120 minutes. 4) With 10,000,000 : 1 solution; the only Hexachlorophene showed the vermicidal effect on Fasciola hepatica. In the case of Bithionol and Dithiazanine iodide the slight stimulation upon the fluke continued for 120 minutes.

• 약학회지
• /
• 제7권1호
• /
• pp.8-12
• /
• 1963

The piperazine salts with 24 kinds of phenols and 6 kinds of organic carboxylic acids were prepared. The test of anthelmintic effect against Ascaris suilla, in vitro, for Hexachlorophene-piperazine salt, Bithionol-piperazine salt, Kainic acid-piperazine salt were also carried out compared with piperazine, Hexachlorophene, Bithionol and Kainic acid.

• 대한수의학회지
• /
• 제8권2호
• /
• pp.68-73
• /
• 1968

Four drugs, bithionol, hexachlorophee, hexachloroethane and tetrachloroethylene, were tested on the in vitro effects of vermicidal and laying capacity inhibitory action to the Eurytrema Pancreaticum of Korean cattle, and the results obtained were as follows : 1) Of the four drugs, bithionol was most effective at both high and low concentrations. 2) The effects of hexachlorophene, hexachloroethane and tetrachloroethylene were less effective than that of bithionol, and the laying activity, at low concentration, was observed to increase at the early stage and decrease gradually thereafter. 3) Tetrachloroethylene was least effective of the four drugs.

• 대한화장품학회지
• /
• 제35권3호
• /
• pp.193-202
• /
• 2009

본 연구에서는 in vivo 광독성 시험을 대체할 수 있는 방법에 관한 연구를 수행하였다. 인체 유래의 섬유아세포를 활용하여 광독성 물질(promethazine, chlorpromazine chlortetracycline, 8-methoxypsoralen, neutral red, bithionol)과 비광독성 물질(cinnamic aldehyde, p-aminobenzoic acid, sodium lauryl sulfate, L-cysteine)을 평가하였다. 세포 생존율은 neutral red uptake (NRU)로 평가하였다. NRU 광독성 시험 결과 bithionol를 제외한 화합물에서 모두 in vivo 실험결과와 유사한 결과를 보였다. 같은 방법으로 화장품 성분인 $Medimin^{(R)}$ A, $Medimin^{(R)}$ D, $LG^{(R)}$ 106W, $Phytoclear^{(R)}$ EL-1, 단삼동 추출물, 미인초 추출물, 산거울 추출물, $Parsol^{(R)}$ MCX와 $Parsol^{(R)}$ 1789를 평가 하였다. 평가 결과 단삼동 추출물을 제외한 원료에서 광독성이 없는 것으로 평가되었다.

• 생약학회지
• /
• 제8권3호
• /
• pp.121-124
• /
• 1977

Aspidium ether extract was obtained from Dryopteris crassirhizoma(Aspidiaceae), which is widely distributed in Korea and its vermicidal effect on the rats infected with Hymenolepis diminuta was investigated. Effects of Aspidium ether extract purchased from the market, several fractions obtained from the seed of Torreya nucifora and bithionol were also compared. Vermicidal effect of Aspidium against Hymenolepis diminuta was found only in one-third of the rats used for investigation and the effect of the fresh Aspidium ether extract was similar to that of Aspidium ether extract purchased from market. However, it was observed that the whole seeds of Torreya nucifera (Torreyaceae) and its residue from which oil fraction was removed as well as bithionol were highly effective against Hymenolepis diminuta.

• Bulletin of the Korean Chemical Society
• /
• 제11권1호
• /
• pp.19-21
• /
• 1990

The crystal structure of Bithionol, $C_{12}H_6Cl_4O_2S$, has been determined from X-ray intensity data measured by Enraf-Nonius CAD-4 diffractometer using graphite-monochromatized $Mo-K\alpha$ radiation. The crystal data as follows; triclinic space group P{\bar{1}}$, a = 8.879(2), b = 10.782(1), c = 8.511(1)${\AA}$, ${\alpha}$ = 115.43(1), ${\beta}$ = 115.22(1), ${\gamma}\;=\;74.44^{\circ}(1)$. ${\mu}\;=\;9.51\;cm^{-1}$, F(000) = 356, Z = 2. Final R value is 0.036 for independent 2669 observed reflections. Each six-membered benzene rings are coplanar within experimesntal errors and the dihedral angle between these planes is $81.28^{\circ}$ (1). The S-(1) and S-C(7) distances are 1.787(2) and 1.791(3)${\AA}$, respectively.

• Toxicological Research
• /
• 제18권3호
• /
• pp.227-232
• /
• 2002

In order to find out the appropriate in vitro method for high correlation with in vivo, we com-pared the sensitivities of phototoxicity (PT) in vitro method between in human keratinocytes, HaCaT cells and in 3T3 fibroblast cells derived from Balb/c mice. Both cells were exposed to six known phototoxic chemicals : promethazine, neutral red, chlortetracycline, amiodarone, bithionol, 8-methoxypsoralen, or non-phototoxic chemical, ALS (ammonium laureth sulfate) and then irradiated with 5 J/$cm^2$ of UVA. Cell viability ($IC_{50}$ ) was measured by neutral red uptake (NRU) assay. The ratio of $IC_{50}$ value of chemicals in the presence and absence of UVA was determined by the cut-off value. The phototoxic potential of test chemicals in NRU assay was determined by measuring the photoirriation factor (PIF) with a cut-off value of 5. In both 3T3 and HaCaT cells, all known phototoxic chemicals were positive (over 5 of PIF value), except that bithionol was found to be non-phototoxic to HaCaT cells, and ALS, non-phototoxic chemical was negative. These results suggest that Balb/c 3T3 cell was more sensitive than HaCaT cell to predict phototoxicity potential.

• 대한수의학회지
• /
• 제11권1호
• /
• pp.49-53
• /
• 1971

The anthelmentic effects of Fuadin (sodium antimony-III-bis-pyrocatechin-disulphonate), Bithionol 2-2-thiobis 4,6-dichlorophenol)), hexachlorethane and carbon tetrachloride were studied on Eurytrema pancreaticum infected in sheep and goats. The evaluation on the effects was based upon the egg reduction in the feces of the experimental animals administered the drugs. The number of eggs per 5gm. of the feces was respectively calculated before the treatments, and the follw-up for the egg reductions was carried out over a period of three or four weeks(calculated twice a week). Oral administration of Bithionol (once a day for 2 succeed days, at the rate of 75 mg per kg of bady weight) and of hexachlorethane (once a day, at the rate of 300ml per kg of body weight) did not reveal any egg reduction in sheep host. Intramuscular injection of carbon tetrachloride with olive oil (once a day, at the rate of 0.05ml per kg of body weight) did not show the egg reduction in goats. Intramuscular injections of Fuadin for 5 days (the dosage schedule was 0.5ml at 1st day, 1.0ml at 2nd and 3rd day, 1.5ml at 4th and 5th day, to amount of 5.5ml) were completely devoid the fluke eggs in the feces of goats on 4 weeks after each treatment. Throughout the studies, Fuadin was only proved its anthelmintic effect to the goats infected with E. pancreaticum. On the other hand, Bithional, hexachlorethane and carbon tetrachloride did not reveal any effect as Fuadin showed.

• Parasites, Hosts and Diseases
• /
• 제21권2호
• /
• pp.150-156
• /
• 1983

1975년부터 1983년까지 치료를 위해 서울의대 기생충학교실로 의뢰된 광절열두조충 충란양성자 5명에 대하여 bithionol, niclosamide 또는 praziquantel을 투여 하고 하제를 사용하여 충체를 얻은바 Diphyllobothrium latum으로 동정되었다. 충자는 서울 및 전남 완도군에 거주하고 있는 10~64세 남(4명) 여(1명)로 그중 2례는 농어를 생식한 경험이 있으며 나머지 3례는 바다생선회를 생식하였으나 그 종류를 기억할 수 없다고 한다. 또 그중 3례는 심한 복통과 소화기증상을 경험하였고, 2례는 편절이 자연배출된 일이 있다고 하나 빈혈소견은 전례에서 관찰되지 않았다. 대편검사에서 발견된 충란은 명 열별로 크기 평균 $59~67{\times} 41~48{\mu}m$이고 황갈색 유란원형 또는 타원형이 있으며 operculum과 abopercular protuberance가 뚜렷하였다. 충체는 4례에서 두절을 포함한 걸이 320~680cm의 완전한 것이 배출되었고 l례에서는 투약후 충체 배출여부를 확인하지 못하고 자연배출된 길이 30cm정도의 체절을 동정에 이용하였다. 특징적인 편절의 형태인 rosette형 자궁, vaginal pore가 cirrus sac에 포함되어 개구된 점, 재정과 cirrus sac의 분리 등으로 보아 Diphyllobothrium latum으로 동정되었다. 본 5례는 우리나라에서 1971년 이후 충체가 확인된 6례에 이어 제 7례-제11례의 인체기생례가 된다.