• Journal of Microbiology and Biotechnology
• /
• 제26권4호
• /
• pp.730-738
• /
• 2016

est19 is a gene from Bacillus sp. K91 that encodes a new esterase. A comparison of the amino acid sequence showed that Est19 has typical Ser-Gly-Asn-His (SGNH) family motifs and could be grouped into the SGNH hydrolase family. The Est19 protein was functionally cloned, and expressed and purified from Escherichia coli BL21(DE3). The enzyme activity was optimal at 60℃ and pH 9.0, and displayed esterase activity towards esters with short-chain acyl esters (C₂-C₆). A structural model of Est19 was constructed using phospholipase A1 from Streptomyces albidoflavus NA297 as a template. The structure showed an α/β-hydrolase fold and indicated the presence of the typical catalytic triad Ser49-Asp227-His230, which were further investigated by site-directed mutagenesis. To the best of our knowledge, Est19 is a new member of the SGNH hydrolase family identified from thermophiles, which may be applicable in the industrial production of semisynthetic β-lactam antibiotics after modification.

• 한국수산과학회지
• /
• 제46권6호
• /
• pp.878-885
• /
• 2013

Seasonal variation in marine macroalgal community structure was examined at the intertidal zones of Heuksando and Hongdo, Shinan, Korea, from July 2008 to May 2009. In total, 86 macroalgal species were identified, including 12 green, 19 brown, and 55 red algae; 67 species at Heuksando and 70 species at Hongdo, were observed. Annual seaweed biomass was 252.44 g dry $wt/m^2$ at Heuksando and 217.67 g dry $wt/m^2$ at Hongdo. The dominant seaweed in importance value (IV > 15) differed between the sites: Sargassum thunbergii at Heuksando and Corallina pilulifera at Hongdo. The vertical distribution pattern of seaweeds from the upper to lower intertidal zones at Heuksando was Gloiopeltis spp., Ulva spp. - S. thunbergii, S. fusiforme, Hildenbrandia rubra - S. thunbergii, C. pilulifera. On the rocky shore of Hongdo, seaweed zonation was distinct: Porphyra yezoensis, Gloiopeltis spp., Ulva spp. - C. pilulifera, S. fusiforme, Myelophycus simplex - Chondrus ocellatus, C. pilulifera, and Carpopeltis affinis. Annual seaweed coverage, richness index (R), evenness index (J'), and diversity index (H') values were greater at Hongdo (41.35%, 12.82, 0.59, and 2.50 respectively) than at Heuksando (31.54%, 11.93, 0.44, and 1.87 respectively), which may indicate that the seaweed community at Hongdo is more stable relative to the one at Heuksando.

• 한국수산과학회지
• /
• 제44권5호
• /
• pp.506-515
• /
• 2011

To elucidate demersal organism community structure, abundance, and biomass by period and region in the Uljin marine ranching area, otter trawl surveys were conducted at six stations from February 2008 to December 2009. During the survey period, a total of 118 species was collected in the Uljin marine ranching area, including 76 pisces, 27 mollusca and 15 crustaceans. The average abundance of individuals was 806 ind./$m^2$ and average biomass was 31,853 g/m2. The numbers of species, species richness, individuals per area and biomass per area were higher in 2009 than in 2008. Cluster and MDS analysis, based on a Bray-Curtis similarity matrix of fourth root transformed data of number of species and individuals per area, showed division into two different groups of demersal organisms in 2008 (Group 1) and one demersal organism community in 2009 (Group 2). From cluster and MDS analysis using the similarity of the demersal community among six stations, the Uljin marine ranching area was divided into Group A (St. 2, St. 4, St. 5 and St. 6) and Group B (St. 1 and St. 3).

• ALGAE
• /
• 제23권4호
• /
• pp.317-326
• /
• 2008

Marine algal flora and community structure were investigated seasonally at three sites in Ilkwang Bay on the southern east coast of Korea from May 2007 to February 2008. Total 103 species including 10 of green algae, 17 of brown algae, 76 of red algae were collected and identified. Among these species, 21 species were found throughout the year. Ulva pertusa, Enteromorpha linza, Grateloupia lanceolata, Chondracanthus intermedia and Caulacanthus ustulatus were distributed dominantly in upper intertidal zone. By contrast, crustose coralline algae, Grateloupia spp., Chondracanthus tenellus, Prionitis cornea and Sargassum spp. occurred predominantly in middle intertidal zone. Grateloupia spp., Sargassum spp., Ecklonia cava and Ulva pertusa were dominant in lower intertidal zone. Annual mean biomass in wet weight was 478.3 g m$^{-2}$. Maximum biomass was recorded in site 1 (731.8 g m$^{-2}$), and minimum was recorded in site 3 (78.5 g m$^{-2}$). The R/P, C/P and (R + C)/P value reflecting flora characteristics were 4.47, 0.59 and 5.06, respectively. Two groups produced by cluster analysis, one including sites 1, 2 and the other including site 3, showed meaningful difference in similarity, each other. Site 3 showed the limited species composition due to inflow of fresh water and absence of solid substratum. However, there was no significant difference between site 1 and site 2. In conclusion, the number of marine algae species and biomass in Ilkwang Bay were markedly reduced comparing with the previous studies. These suggest that a solution for reconstruction of the poor marine algal vegetation is considerably demanded.

• 한국항해항만학회지
• /
• 제30권4호
• /
• pp.309-314
• /
• 2006

Community structure and biodiversity of benthic marine algae were studied in the rocky shore of Phohang New Port, Yeoungil Bay. A total of 79 species of marine algae including 8 Chlorophyta, 23 Phaeophyta and 48 Rhodophyta are listed. The dominant algal species were Corallina pilulifera in all seasons, and Sargassum homeri in winter. Lomentaria catenata, Lomentaria hakodatensis, Grateloupia laceolata, Sargassum thunbergii, Chondria crassicaulis, Ulva pertusa, and Hypnea charoides were subdominant at different seasons. The algal biomass per unit area of the benthic algal community in Pohang New Port under construction was 14506 - 398.93 g dry wt m-2, which means that its annually averaged value is 273.45 g dry wt m-2 . The seasonal change in algal biomass showed highs in winter and spring, and lows in summer and autumn. The algal species diversity (H') based on algal biomass was 2.07 annually in average, with 2.42 in winter, 2.65 in spring, 1.79 in summer and 1.43 in autumn. It was high in winter and spring seasons and low in summer and autumn seasons. It is caused by that spring and winter are the profitable growth time for algae and species components in this study were dominated by the annual or seasonal rather than perennial plants.

• 한국해양바이오학회지
• /
• 제14권2호
• /
• pp.102-111
• /
• 2022

Beakryoung-do is located in the north along the western coast of South Korea. Owing to its geographical limitations, not many studies have been conducted on marine algae in this region. This study aims to investigate the marine algal flora and the community structure in the intertidal and subtidal zones of Nampo-ri, Beakryoung-myeon, Ongin-gun, Incheon regions of South Korea. The study was conducted from August 2015 to June 2016 during all seasons. A total of 95 algal species, including 7 green algae, 12 brown algae, and 76 red algae were collected and identified; 52 algal species were found in the intertidal zone and 83 in the subtidal zone. The vertical distribution of algae from upper to lower intertidal zone was Neorhodomela aculeata - Caulacanthus okamurae, Gelidiophycus freshwateri - Neorhodomela aculeata, Caulacanthus okamurae - Sargassum thunbergii, Caulacanthus okamurae. Findings indicated that Sargassum thunbergii and Neorhodomela aculeata were the biomass-dominant species in the intertidal zone and Corallina officinalis and Undaria pinnatifida were biomass-dominant in the subtidal zone. Average values of marine algae biomass in the intertidal and subtidal zones were 252.6 g wet weight m^-2 and 291.9 g wet weight m^-2, respectively. Further, 38 new species were found in Beakryoung-do, with 25 of them specifically found in the subtidal zone. More studies on marine algal flora in the subtidal zone are needed to understand the changes in marine flora along the western coast.

• Proceedings of the National Institute of Ecology of the Republic of Korea
• /
• 제4권2호
• /
• pp.86-94
• /
• 2023

Climate change is more rapid in the Arctic than elsewhere in the world, and increased precipitation and warming are expected cause changes in biogeochemical processes due to altered microbial communities and activities. It is crucial to investigate microbial responses to climate change to understand changes in carbon and nitrogen dynamics. We investigated the effects of increased temperature and precipitation on microbial biomass and community structure in dry tundra using two depths of soil samples (organic and mineral layers) under four treatments (control, warming, increased precipitation, and warming with increased precipitation) during the growing season (June-September) in Cambridge Bay, Canada (69°N, 105°W). A phospholipid fatty acid (PLFA) analysis method was applied to detect active microorganisms and distinguish major functional groups (e.g., fungi and bacteria) with different roles in organic matter decomposition. The soil layers featured different biomass and community structure; ratios of fungal/bacterial and gram-positive/-negative bacteria were higher in the mineral layer, possibly connected to low substrate quality. Increased temperature and precipitation had no effect in either layer, possibly due to the relatively short treatment period (seven years) or the ecosystem type. Mostly, sampling times did not affect PLFAs in the organic layer, but June mineral soil samples showed higher contents of total PLFAs and PLFA biomarkers for bacteria and fungi than those in other months. Despite the lack of response found in this investigation, long-term monitoring of these communities should be maintained because of the slow response times of vegetation and other parameters in high-Arctic ecosystems.

• 한국산림과학회지
• /
• 제87권2호
• /
• pp.173-178
• /
• 1998

야생 차나무집단의 임분구조와 현존량의 관계를 파악하기 위하여 지역, 위도 등을 고려하여 12개 야생 차나무 집단을 선정하여 임분구조와 현존량을 조사하였다. 차나무 집단별 평균 근원직경은 집단별 평균 수령과 유의적인 상관관계가 있었으나 집단별 평균 수고, 밀도, 근원단면적은 집단별 평균수령과 유의적인 상관관계가 없었다. 관목층인 차나무 집단의 밀도와 근원단면적은 교목층의 흉고단면적과 유의적인 상관관계가 없었다. 차나무의 표본목 측정 결과 근원직경이 증가함에 따라 줄기와 가지, 뿌리의 건중량은 지수함수적으로 증가하였으며, 1년생 잎과 소지의 건중량은 직선적으로 증가하였다. 2년생 이상 잎 건중량의 경우 근원직경과 상관관계가 없었다. 근원직경이 증가함에 따라 줄기와 가지, 뿌리의 건중량이 증가하는 것은 근주당 줄기의 수가 증가하기 보다는 좋기의 평균 직경과 수고가 증가하기 때문이었다. 차나무 집단의 임목 전체 현존량은 1,162~11,474kg/ha, 주 이용 대상인 1년생 잎의 현존량은 165~1,341kg/ha로서 집단간 큰 차이가 있었으며, T/R율은 1.5~2.8의 범위를 보였다. 축적기관인 줄기와 가지, 뿌리의 현존량은 평균 수령, 평균 근원직경, 밀도와는 독립적이었으나 근원단면적과 유의적인 상관관계가 있었다. 생산기관인 1년생 잎과 소지의 현존량은 평균수령, 평균 근원직경과는 독립적이었으나, 근원단면적, 밀도와 유의적인 상관관계가 있었다.

• 한국수산과학회지
• /
• 제50권5호
• /
• pp.553-560
• /
• 2017

Fluctuations in the abundance and species composition of fishes in the coastal waters of Geumo-do, Yeosu, Jeollanam-do, Korea were investigated from 2002 to 2005. A gill net with entrances on both sides was used to collect a total of 469 individuals (55,180 g) of 3 orders, 18 families, and 28 species. The dominant species was Sebastes inermis (60 individuals, 5,670 g), followed by Neoditrema ransonneti (46 individuals, 2,290 g), and Pseudaesopia japonica (38 individuals, 3,080 g). The highest number of individuals was found in 2004, while the biomass was highest in 2005. The diversity index was highest in 2004 (H'=2.703) and lowest in 2002 (H'=2.580). The dominance index was highest in 2004 (D=3.983) and lowest in 2002 (D=3.404). The richness index was highest in 2003 (RI=0.337) and lowest in 2002 (RI=0.261). Changes in temperature and salinity affected the community structure and the location of spawning grounds. The number of individuals and total biomass increased with changes in temperature and salinity each year.

• ALGAE
• /
• 제23권2호
• /
• pp.141-150
• /
• 2008

Populations of Codium amplivesiculatum were found in association with rhodolith beds. We present population trends of C. amplivesiculatum from surveys conducted in the southwestern Gulf of California based on monthly sampling from June 1999 to August 2000. Surveys were conducted at 2 sites in the San Lorenzo Channel at depths of 8 - 12 m. The parameters measured were percent cover, biomass and structure in relation to size of the thalli. From each thallus the following measurements were taken: length and diameter, proportion of each thallus with reproductive structures and the degree of fragmentation (measured as number of small thalli). Mean biomass and cover varied seasonally with the maximum (1036.6 gm$^{-2}$ and 100% cover) present in June 2000 and the minimum in winter (0 gm$^{-2}$ and 0% cover). The longest fronds (3 m) occurred during July 1999 and August 2000, while the maximum diameters of 1.0 cm were present during October and November 1999. There were no significant linear relationships between length and diameter of the fronds, suggesting independent growth. Reproductive structures were common in June and July 1999 with monoic and dioic thalli present. Most fronds were monoic, and this is the first record of this feature for this species. Fragmentation occurred in September, and may be associated with stress from early gametangial reproduction and the combination of high temperatures and low nutrients. A hypothetical life cycle for the species is presented.