• Journal of Korean Society of Forest Science
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• v.85 no.3
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• pp.443-452
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• 1996

A study has been made to estimate biomass and NPP based on equation form of $Wt=aD^bH^c$ for Quercus variabilis and Quercus mongolica natural stands(Mean age; 67, 62yrs old) in Chungju. Equation form of $Wt=aD^bH^c$ was more adequate than $Wt=a(D^2H)^b$ and $Wt=aD^b$ for the estimation of the biomass and NPP. Individual biomass was compared using a paired t-test by tree component which showed no significant differences. Total aboveground biomass of Quercus mongolica was 130.6 t/ha and that of Quercus variabilis was 137.4 t/ha. Biomass of Q. mongolica was composed of foliage 5.1 t/ha(3.9%), dead branch 3.5 t/ha(2.7%), live branch 29.7 t/ha(23.0%), bolebark 16.2 t/ha(12.5%), and bolewood 74.9 t/ha(58.0%), and that of Q. variabilis was composed of foliage 3.8 t/ha(2.9%), dead branch 2.9 t/ha(2.2%), live branch 24.3 t/ha(18.4%), bolebark 20.4 t/ha(15.5%), and bolewood 80.4 t/ha(61.0%). Net primary production was 10.0 t/ha/yr in the Q. mongolica stand and 8.6 t/ha/yr in the Q. variabilis stand, respectively. Net primary production of Quercus forest in Chungju was very close to the mean NPP of the broadleaved forest of temperate zone.

• Journal of Korean Society of Forest Science
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• v.88 no.2
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• pp.266-272
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• 1999

Aboveground biomass and nutrient contents of a 31-year-old pitch pine(Pinus rigida) and a 31-year-old Japanese larch(Larix leptolepis) plantations were measured in the Chungbu Forest Experiment Station, Kyunggi Province. Aboveground biomass was 170.2ton/ha in the pitch pine and 87.2ton/ha in the Japanese larch plantations. Aboveground biomass difference between both plantations was due to the difference of stand density. Aboveground biomass in both plantations was allocated as follows : stemwood>branch>stembark>needle. The concentrations of all nutrients(N, P, K, Ca, Mg) were generally higher in the Japanese larch needle than in the pitch pine because of high nutrient uptake characteristics of larch compared with pine tree species. The nutrient concentration in different tree tissues in both tree species decreased in the order of needle>branch>stembark>stemwood. Nutrient contents of aboveground biomass were : N, 335.9 ; P, 40.4 ; K, 121.4 ; Ca, 188.6 ; Mg, 93.8kg/ha in the pitch pine plantation, while nutrient contents in the Japanese larch plantation were : N, 226 ; P, 11.5 ; K, 72.9 ; Ca, 75.7 ; Mg, 37.1kg/ha. The nitrogen use efficiency calculated as the biomass produced by one unit of nitrogen was higher in the pitch pine than in the Japanese larch plantations. This result suggests that pine with high nitrogen use efficiency could be adapted in lower site productivity area compared with larch tree species.

• Journal of Wetlands Research
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• v.9 no.2
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• pp.33-43
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• 2007

Distribution area, actual vegetation map, standing crop biomass, relative growth, and primary productivity of Salix spp.were investigated to provide the basic data necessary for conservation and ecotechnological application of Salix community in the Upo wetland, Changryeong County, Gyeongsangnamdo Province, Korea from April 2005 to April 2006. 1. Among seven Salix species, the dominant species was S. nipponica and S. glandulosa was subdominant. There were six kinds of Salix communities such as S. nipponica, S. glandulosa, S. koreensis, S. nipponica-S. glandulosa, S. glandulosa-S. nipponica, and S. nipponica-S. koreensis. 2. Distribution area of S. nipponica community was largest as 28.46 ha among Salix communities and those of S. nipponica-S. glandulosa community, S. glandulosa-S. nipponica community, S. nipponica-S. koreensis community, S. glandulosa community, and S. koreensis community were 6.12 ha, 6.12 ha, 2.92 ha, 1.86 ha, and 0.81 ha, respectively. 3. The tree age, tree height, and DBH of S. nipponica were 5~13year, 4.1~7.2 m, and 3.0~14.0 cm and those of S. glandulosa were 2~36year, 3.5~10.1 m, and 3.2~26.0 cm, respectively. 4. The standing crop of S. nipponica was 408 ton and that of S. glandulosa was 336 ton in the study area. 5. The productivity of S. nipponica was $235g\;m^{-2}yr^{-1}$ and the annual production was 86.4 ton, and those of S. glandulosa were $1,006g\;m^{-2}yr^{-1}$ and 80.3 ton in the study area.

• Korean Journal of Agricultural Science
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• v.38 no.4
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• pp.785-791
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• 2011

This study was carried out to analyze the effect of the farm management and productivity increase of cabbages growing in the plastic house based on application of green manure crops such as silage corn, haussolgo (Sorghum bicolar L. Moench), hairy vetch, etc. According to the effect of green manure crop grown in summer season, the biomass amount of the silage corn was the highest; 7,630 kg per 10a, the next was haussolgo, 5,620 kg per 10a. In terms of the fertilizer ingredients, the first of forage soybean was 3.84% of nitrogen, whereas hairy vetch was 1.74% of phosphate and kalium, 4.74%. Productivity increase of cabbages was the highest in the haussolgo plot of which the yield of showed 10,090 kg per 10a and the farm household income would be worth 8,053 thousand won. By growing forage crops in the winter season, the biomass amount was the highest in the mixed sowing plot with rye (50%) and hairy vetch (50%) of 3,590 kg per 10a, whereas the productivity in the mixed seeding with rye (70%) and hairy vetch (30%) was highest, 6,249 kg per 10a and the farm household income would be worth 7,387 thousand won. Judging from these results, more practical on-farm research on applying different green manure crops as a basal fertilizer with cabbage in the plastic house should be considered to analyze the farm management and the farm household income at different sites.

• The Korean Journal of Ecology
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• v.24 no.6
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• pp.377-380
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• 2001

Herbivory is an important selective forces on plants by reducing the ability of the plant to compete with its neighbors and to produce offspring. Oak nut weevil(Mechoris ursulus Roelofs) females lay eggs in unmatured acorns and cut off the branch of oviposited acorns. To investigate the influences of branch-cutting behavior of oak nut weevils on oak production, we surveyed the horizontal and vertical distribution ranges of damaged oaks and depth of sites of overwintering larvae and quantified the amounts of biomass and acorn removed by the weevils on deciduous oak species. All of the endemic oak species in Korea were damaged by oak nut weevils in all of the study sites including Mt. Halla and Mt. Seorak. The upper limit of vertical distribution of damaged oaks by the weevils raried from 700 m to 900 m. Mostly their larvae were found in soil from 8.1 cm to 10.0 cm depth in winter. Percentage of annual net primary productivity($720g{\cdot}m^{-2}{\cdot}day^{-1}$) of oak species removed by the weevils was about 7%, which is higher than the percentage removed by all the herbivores in typical temperate forest(5%) and equivalent to that by all the herbivores in tropical forest. The predation percentage of annual acorn production by oak nut weevil was 27% in Q. mongolica and 33% in Q. acutissima. This results indicate that the branch-cutting behavior of oak nut weevil may be the most important factor regulating oak population and affecting other predators dependent on acorns.

• Journal of Marine Bioscience and Biotechnology
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• v.10 no.1
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• pp.26-33
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• 2018

Fucoxanthin has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. Owing to its a wide range of applications and potentials, commercial production of fucoxanthin from algae has been attracted many attentions. Although, most of seaweeds and diatoms contain fucoxanthin as major carotenoid contents, low productivity of fucoxanthin still hinder the industrial application. Here, we newly isolated and identified indigenous marine diatom Odontella sp. BS-003 as a resource of fucoxanthin production. The characterization, optimization and production of the fucoxanthin, along with other bioactive compound omega-3 fatty acid from odontella sp. BS-003 were analyzed in this study, and the results represented optimal culture condition (two-fold silicate containing F/2 medium) significantly enhanced the algal biomass productivity. The maximum biomass (1.83 g/L), fucoxanthin (3.88 mg/g), along with omega-3 fatty acid (10 %, w/w) were obtained from the 10 L of photobioreactor. Based on the results, it is speculated that the microalga Odontella sp. BS-003 can be a promising natural resource for the production of bioactive compounds.

• The Korean Journal of Ecology
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• v.1 no.2
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• pp.57-65
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• 1977

The biomass and net production of alder and oak trees was estimated by allometric method. The productivity of the two stands of alder and oak was obviously different judging from the rate of photosynthesis productive structure and vertical distribution of light. The amounts of net photosynthesis under the saturated light were 2.31, 1.42mg $CO_2/dm^2\cdot$hr. in the sun and shade leaves of alder tree and 1.58, 0.84mg $CO_2/dm^2\cdot$hr in that of the oak, respectively. Total annual respiration loss calculated from the respiration measured at $25^{\circ}C$ and the mean air temperature from every 10 days were 13.56ton/ha.yr in the alder stand and 19.83 ton/ha.yr in the oak. The productive structure and the vertical distribution of light in the stand were assumedly more effective to produce dry matter in the oak stand than in the alder. The biiomasses measured in 1975 and 1976 were 51.51 and 56.82 ton/ha in the alder stand and 73.35, 86.77 ton/ha in the oak one, respectively. Annual net production and gross production were 8.56 and 22.12 ton/ha.yr in the alder stand but those were 17.90 and 37.74 ton/ha.yr in the oak stand. The ratios of respiration to gross procution (R/Pg) were prespectively 0.61 and 0.53 inthe alder and oak stands. Efficiencies of solar energy utilizaztion of net production during the growing season(May-Oct.) were 0.67 and 1.40% and those of gross production were 1.72 and 2.94% in the alder and oak stands respectively.

• Journal of Marine Bioscience and Biotechnology
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• v.11 no.1
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• pp.14-22
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• 2019

Omega-3 fatty acids and carotenoids, which are known as representative high-value substances derived from microalgae, are being studied from various diatoms. Most of the diatoms contain fucoxanthin and omega-3 fatty acid. Fucoxanthin produced by diatom has been reported as bioactive compounds exhibiting strong antioxidant, anticancer and anti-inflammatory activities. However, the low growth rate and fucoxanthin content of diatoms are one of the big obstacles to the industrial application. In this study, indigenous marine diatom Achnanthidium sp. BS-001 was isolated for a candidate of fucoxanthin producer. Light intensity and temperature for the culture of Achnanthidium sp. BS-001 were optimized on PhotoBiobox. Optimization of silicate concentration for increasing BS-001 biomass productivity was confirmed in F/2 medium with various concentration of sodium silicate. As a result, condition of light intensity, temperature, and silicate concentration for optimal cultivation were $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $18^{\circ}C$ and 0.106 mM, respectively. Maximum biomass productivity reaches to $154.3mg{\cdot}L^{-1}{\cdot}day^{-1}$, and then the content of omega-3 fatty acids and fucoxanthin were $19.4mg{\cdot}g^{-1}$, $9.05mg{\cdot}g^{-1}$, respectively. These results indicate that Achnanthidium sp. BS-001 has the potential to be used as a source of omega-3 fatty acids and fucoxanthin.

• Journal of Microbiology and Biotechnology
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• v.32 no.10
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• pp.1325-1334
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• 2022

Global warming has accelerated in recent decades due to the continuous consumption of petroleum-based fuels. Cyanobacteria-derived biofuels are a promising carbon-neutral alternative to fossil fuels that may help achieve a cleaner environment. Here, we propose an effective strategy based on the large-scale cultivation of a newly isolated cyanobacterial strain to produce phycobiliprotein and biodiesel, thus demonstrating the potential commercial applicability of the isolated microalgal strain. A native cyanobacterium was isolated from Goryeong, Korea, and identified as Pseudanabaena mucicola GO0704 through 16s RNA analysis. The potential exploitation of P. mucicola GO0704 was explored by analyzing several parameters for mixotrophic culture, and optimal growth was achieved through the addition of sodium acetate (1 g/l) to the BG-11 medium. Next, the cultures were scaled up to a stirred-tank bioreactor in mixotrophic conditions to maximize the productivity of biomass and metabolites. The biomass, phycobiliprotein, and fatty acids concentrations in sodium acetate-treated cells were enhanced, and the highest biodiesel productivity (8.1 mg/l/d) was achieved at 96 h. Finally, the properties of the fuel derived from P. mucicola GO0704 were estimated with converted biodiesels according to the composition of fatty acids. Most of the characteristics of the final product, except for the cloud point, were compliant with international biodiesel standards [ASTM 6761 (US) and EN 14214 (Europe)].

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.6
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• pp.482-493
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• 2005

Biomass is originally photosynthesized from inorgainic compounds such as $CO_2$, minerals, water and solar energy. Recent studies have shown that anaerobic bacteria have the ability to convert recalcitrant biomass such as cellullosic or chitinoic materials to useful compounds. The biomass containing agricultural waste, unutilized wood and other garbage is expected to utilize as feed, food and fuel by microbial degradation and other metabolic functions. In this study we isolated several anaerobic, cellulolytic and chitinolytic bacteria from rumen fluid, compost and soil to study their related enzymes and genes. The anaerobic and cellulolytic bacteria, Clostridium thermocellum, Clostridium stercorarium, and Clostridium josui, were isolated from compost and the chitinolytic Clostridium paraputrificum from beach soil and Ruminococcus albus was isolated from cow rumen. After isolation, novel cellulase and xylanase genes from these anaerobes were cloned and expressed in Escherichia coli. The properties of the cloned enzymes showed that some of them were the components of the enzyme (cellulase) complex, i.e., cellulosome, which is known to form complexes by binding cohesin domains on the cellulase integrating protein (Cip: or core protein) and dockerin domains on the enzymes. Several dockerin and cohesin polypeptides were independently produced by E. coli and their binding properties were specified with BIAcore by measuring surface plasmon resonance. Three pairs of cohesin-dockerin with differing binding specificities were selected. Two of their genes encoding their respective cohesin polypeptides were combined to one gene and expressed in E. coli as a chimeric core protein, on which two dockerin-dehydrogenase chimeras, the dockerin-formaldehyde dehydrogenase and the dockerin-NADH dehydrogenase are planning to bind for catalyzing $CO_2$ reduction to formic acid by feeding NADH. This reaction may represent a novel strategy for the reduction of the green house gases. Enzymes from the anaerobes were also expressed in tobacco and rice plants. The activity of a xylanase from C. stercorarium was detected in leaves, stems, and rice grain under the control of CaMV35S promoter. The digestibility of transgenic rice leaves in goat rumen was slightly accelerated. C. paraputrificum was found to solubilize shrimp shells and chitin to generate hydrogen gas. Hydrogen productivity (1.7 mol $H_2/mol$ glucos) of the organism was improved up to 1.8 times by additional expression of the own hydrogenase gene in C. paraputrficum using a modified vector of Clostridiu, perfringens. The hydrygen producing microflora from soil, garbage and dried pelletted garbage, known as refuse derived fuel(RDF), were also found to be effective in converting biomass waste to hydrogen gas.