• Title/Summary/Keyword: Biology and Life Science

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CRISPR base editor-based targeted random mutagenesis (BE-TRM) toolbox for directed evolution

  • Rahul Mahadev Shelake;Dibyajyoti Pramanik;Jae-Yean Kim
    • BMB Reports
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    • v.57 no.1
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    • pp.30-39
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    • 2024

  • Directed evolution (DE) of desired locus by targeted random mutagenesis (TRM) tools is a powerful approach for generating genetic variations with novel or improved functions, particularly in complex genomes. TRM-based DE involves developing a mutant library of targeted DNA sequences and screening the variants for the desired properties. However, DE methods have for a long time been confined to bacteria and yeasts. Lately, CRISPR/Cas and DNA deaminase-based tools that circumvent enduring barriers such as longer life cycle, small library sizes, and low mutation rates have been developed to facilitate DE in native genetic environments of multicellular organisms. Notably, deaminase-based base editing-TRM (BE-TRM) tools have greatly expanded the scope and efficiency of DE schemes by enabling base substitutions and randomization of targeted DNA sequences. BE-TRM tools provide a robust platform for the continuous molecular evolution of desired proteins, metabolic pathway engineering, creation of a mutant library of desired locus to evolve novel functions, and other applications, such as predicting mutants conferring antibiotic resistance. This review provides timely updates on the recent advances in BE-TRM tools for DE, their applications in biology, and future directions for further improvements.

  • https://doi.org/10.5483/BMBRep.2023-0086 인용 PDF

Survey of Awareness and Concept of Insects in Korea

  • Bae, Sung-Min;Choi, Jae-Bang;Shin, Tae-Young;Woo, Soo-Dong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.27 no.2
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    • pp.317-321
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    • 2013

  • To investigate the degree of individuals' concept and awareness of insects, a survey study was conducted with students and adults living in Korea. The misconception rate for insects was about 50% for both students and adults, but it was lower for students and people who had experienced insect-related events than for adults and those who had not. The highest misconception rate was obtained in answer to a question about the basic structure of an insect. Most people had a high awareness of insects. Significant differences and correlations for the awareness of insects were found between students and adults, men and women, people who had experienced insect-related events and those who had not. The experience of an insect-related event most influenced awareness of insects. These results suggest that increasing people's interest in insects and utilizing insects in treatment situations may be beneficial for the field of mental healthcare.

  • https://doi.org/10.7852/ijie.2013.27.2.317 인용 PDF KSCI

Remodeling of host glycoproteins during bacterial infection

  • Kim, Yeolhoe;Ko, Jeong Yeon;Yang, Won Ho
    • BMB Reports
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    • v.54 no.11
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    • pp.541-544
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    • 2021

  • Protein glycosylation is a common post-translational modification found in all living organisms. This modification in bacterial pathogens plays a pivotal role in their infectious processes including pathogenicity, immune evasion, and host-pathogen interactions. Importantly, many key proteins of host immune systems are also glycosylated and bacterial pathogens can notably modulate glycosylation of these host proteins to facilitate pathogenesis through the induction of abnormal host protein activity and abundance. In recent years, interest in studying the regulation of host protein glycosylation caused by bacterial pathogens is increasing to fully understand bacterial pathogenesis. In this review, we focus on how bacterial pathogens regulate remodeling of host glycoproteins during infections to promote the pathogenesis.

  • https://doi.org/10.5483/BMBRep.2021.54.11.129 인용 PDF KSCI

Expression of Pseudorabies Virus Glycoproteins gB, gC and gD using Insect Expression System

  • Yun, Bit Na Rae;Gwak, Won Seok;Lee, Ji Hoon;Woo, Soo Dong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.35 no.2
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    • pp.118-122
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    • 2017

  • Porcine pseudorabies virus (PRV) causes the Aujeszky's disease (AD) which is economically important disease in the swine industry worldwide. Killed or live vaccines have been used to control this disease, but their efficacy and side effects remain problems to be solved. To solve these problems, in this study, production of recombinant PRV glycoprotein gB, gC and gD was investigated in insect expression system. Glycoprotein gB, gC and gD are regarded as the major immunogenic antigens in PRV. Abundant production and immunogenicity of glycoprotein gB, gC and gD were confirmed by SDS-PAGE and Western blot analysis, respectively. Optimal infection dose and time were also determined for the production of each recombinant PRV glycoprotein. Confirmation of glycosylation of recombinant gB, gC and gD suggested their usefulness as antigens for the development of diagnosis kit or vaccines for Aujeszky's disease.

  • https://doi.org/10.7852/ijie.2017.35.2.118 인용 PDF KSCI