• Title/Summary/Keyword: Biology and Life Science

Search Result 4,241, Processing Time 0.038 seconds

Nucleopolyhedrovirus Induces Suppressor of Cytokine Signaling in the Beet Armyworm, Spodoptera exigua

  • Noh Mi-Young;Jo Yong-Hun;Kim Seon-Am;Lee Yong-Seok;Bang In-Seok;Kim Seon-Gon;Park Jong-Dae;Chun Jae-Sun;Seo Sook-Jae;Han Man-Deuk;Kim Ik-Soo;Han Yeon-Soo
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.12 no.2
    • /
    • pp.63-67
    • /
    • 2006

  • Suppressor of cytokine signaling (SOCS) is known to playa key role as a negative feedback regulator in JAK/STAT signaling cascade in innate immunity. Our laboratory has recently been interested in elucidating the interactions between Spodoptera exigua (Se) and SeNPV. This context leads us to clone and characterize SeSOCS that may have important functions in response to SeNPV infection. Using the RT-PCR and TA cloning approach, we found a partial fragment (416 bp) of SeSOCS. Blast search and multiple alignment data showed that it has a homology to various insects such as Anopheles gambiae (78%), Aedes aegypti (75%), Drosophila melanogastar (77%), Mus musculus (69%), and Homo sapiens (69%). Temporal induction patterns of SeSOCS were analysed after being immune-challenged with either NPV or laminarin. It showed that the level of SeSOCS mRNA was strongly induced in a biphasic manner in response to SeNPV and laminarin, respectively. It seems that SOCS, a negative regulator of JAK/STAT signaling system is also present in S. exigua and may playa role in innate immunity albeit its precise role should be further elucidated at the molecular and cellular level in the early phase of SeNPV infection in larvae.

  • PDF KSCI

Construction of the Full-length cDNA Library and Selection of Diapause-Associated cDNA Clones from Bombyx mori Diapausing Eggs and Diapause-Activated Eggs

  • Hwnag, Jae-Sam;Go, Hyun-Jeong;Goo, Tae-Won;Yun, Eun-Young;Choi, Kwang-Ho;Seong, Su-Il;Kim, Sung-Kuk;Lee, Sang-Mong;Kim, Iksoo;Kim, Nam-Soon;Kang, Seok-Woo
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.9 no.1
    • /
    • pp.145-148
    • /
    • 2004

  • As an initial step to define the molecular mechanism of diapause during embryogenesis of the silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause-activated eggs were compared by differential expression using cDNA microarray. Twenty-four individual cDNA clones were identified. Amomg them, ten genes including alcohol dehydrogenase, dead box-l, cytochrome oxidase subunit I and 18 wheeler showed increased expression in the diapause-activated eggs. The rest of fourteen genes showed increased expression in diapausing eggs.

  • PDF KSCI

Potentiation of serotonin N-acetyltransferase activity by protein kinase A and C involves two specific phosphorylation sites.

  • Park, Bo-Hwa;Chae, Hee-Don;Park, Tae-Ju;Kang, Shin-Sung;Kim, Kyong-Tai
    • Proceedings of the Korean Biophysical Society Conference
    • /
    • 2001.06a
    • /
    • pp.48-48
    • /
    • 2001

  • Gene expression and protein levels of serotonin N-acetyltransferase (AA-NAT, EC2.3.1.87) directly control the diurnal production of the melatonin and correlate with enzyme activity. Effects of protein phosphorylation on the AA-NAT activity were investigated in rat pineal glands and COS-7 cells transiently transfected with AA-NAT cDNA.(omitted)

  • PDF