• Proceedings of the Korean Biophysical Society Conference
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• 1999.06a
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• pp.44-44
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• 1999

Yeast cytochrome c peroxidase (CcP) was cloned and overexpressed in E. coli, and purified by a Ni$^{2＋}$-affinity column. HoloCcP was obtained by reconstituting apoCcP with Mn$^{3+}$-protoporphyrin IX (MnPP). Electron paramagnetic resonance (EPR) spectra of spin-labeled holoCcP showed a slightly more immobilized signal than spin-labeled apoCcP.(omitted)

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.5
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• pp.432-443
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• 2005

Microarray technology has contributed Significantly to the understanding of bacterial genetics and transcriptional regulation. One neglected aspect of this technology has been optimization of microarray-generated signals and quality of generated information. Full genome microarrays were developed for Clostridium acetobutylicum through spotting of PCR products that were designed with minimal homology with all other genes within the genome. Using statistical analyses it is demonstrated that Signal quality is significantly improved by increasing the hybridization volume. possibly increasing the effective number of transcripts available to bind to a given spot, while changes in labeled probe amounts were found to be less sensitive to improving signal quality. In addition to Q-RT-PCR, array validation was tested by examining the transcriptional program of a mutant (M5) strain lacking the pSOL1 178-gene megaplasmid relative to the wildtype (WT) strain. Under optimal conditions, it is demonstrated that the fraction of false positive genes is 1% when considering differentially expressed genes and 7% when considering all genes with signal above background. To enhance genomic-scale understanding of organismal physiology, using data from these microarrays we estimated that $40{\sim}55%$ of the C. acetobutylicum genome is expressed at any time during batch culture, similar to estimates made for Bacillus subtilis.

• Korean Journal of Optics and Photonics
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• v.19 no.2
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• pp.103-107
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• 2008

Unlike conventional optical coherence tomography systems based on Michelson interferometer, we suggest a common-path OCT system, which does not include a separated configuration between reference signal and sample signal. We optimize the refractive index of partial reflecting probe to induce a balanced intensity of the reference signal. At the end of the probe, convex lens was optimally fabricated to get images of biological samples in the position of focus. Using the experimental system, we could get 2-D images of various biological samples.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.35 no.4
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• pp.412-417
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• 2022

Capacitive-type humidity sensors with a high sensitivity and fast response/recovery times have attracted a great attention in non-contact respiration biological signal monitoring applications. However, complicated fabrication processes involving high-temperature heat treatment for the hygroscopic film is essential in the conventional ceramic-based humidity sensors. In this study, a non-toxic ceramic/metal halide (BaTiO₃(BT)/NaCl) humidity sensor was prepared at room temperature using a solvent-free aerosol deposition process (AD) without any additional process. Currently prepared BT/NaCl humidity sensor shows an excellent sensitivity (245 pF/RH%) and superior response/recovery times (3s/4s) due to the NaCl ionization effect resulting in an immense interfacial polarization. Furthermore, the non-contact respiration signal variation using the BT/NaCl sensor was determined to be over 700% by maintaining the distance of 20 cm between the individual and the sensor. Through the AD-fabricated sensor in this study, we expect to develop a non-contact biological signal monitoring system that can be applied to various fields such as respiratory disease detection and management, infant respiratory signal observation, and touchless skin moisture sensing button.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.11
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• pp.265-268
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• 1995

Signal averaging technique to improve signal-to-noise ratio has widely been used in various fields, especially in electrophysiology. Estimation of the EP(evoked potential) signal using the conventional averaging method fails to correctly reconstruct the original signal under EEG(electroencephalogram) noise especial]y when the latency times of the evoked potential are not identical. Therefore, a technique based on the bispectrum averaging was proposed for recovering signal waveform from a set o noisy signals with variable signal dalay. In this paper an improved bispectrum estimation technique of the RP signal is proposed using a confidence thresholding of the EP signal in frequency domain in which energy distribution of the EP signal is usually not uniform. The suggested technique is coupled with the conventional bispectrum estimation technique such as least square method and recursive method. Some results with simulated data and real EP signal are shown.

• Journal of Biomedical Engineering Research
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• v.5 no.2
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• pp.133-142
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• 1984

The EMG signal can be considered as a signal source that expresses the intention of man because it is a electrical signal generated when the man contracts muscles. For proportional control of prostheses, the control signal proportional to the mousle contraction level must be estimated. Typically a foul-wave rectifier and low-pass filter are used to estimate the proportional control signal from the EMG signal. In this paper, it is proposed to use a logarithmic transformation and a linear minimum mean square error estimator. A logarithmic transformation maps the myoelectric signal into an additive control signal-plus-noise domain and the Kalman filter is used to estimate the control signal as a linear minimum mean square error estimator. The performance of this estimator is verified by the computer simulation and the estimator is applied to the EMG obtained from the biceps brachii muscle of normal subjects.

• Journal of Sensor Science and Technology
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• v.20 no.2
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• pp.107-113
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• 2011

LSPR(Localized Surface Plasmon Resonance) sensor measures the refractive index change on the sensor surface. The detection of biological reaction with the unknown refractive index needs to be converted into the signal sensitivity for the refractive index change for comparison with other measurements. To find the signal sensitivity, the three steps of signal processing are proposed, which are signal modeling, signal calibration and signal normalization of LSPR sensor. The detected signal of biotin-streptavidin interaction has been converted into unit of [RU](Resonance Unit) using the proposed method. The converted signal directly can be compared with the other sensors including commercialized one.

• Journal of Sensor Science and Technology
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• v.12 no.3
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• pp.121-127
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• 2003

In this study, we carried out a study for implementation of the pre-amplifier and the digital signal processing part for the potable EEG biofeedback system. As we consider characteristics of the EEG signal, we designed the pre-amplifier to obtain the EEG signal to be reduced noise signal. Because the EEG signal include EOG, EMG, ECG signals etc, it is difficult to analyze of the EEG signal. Therefore, we developed DSP board and operation program which was embed the LMS adaptive filter algorithm and operate with the pre-amplifier in the real time. The simulation signal and pure EEG signal is used in the experiment. As the result, we confirmed good efficiency of developed system and possibility of application to the portable EEG biofeedback system.

• Journal of Applied Biological Chemistry
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• v.53 no.2
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• pp.105-107
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• 2010

To overcome the limitation of E. coli expression system such as inclusion body formation and disulfide bond failure, we tried to express the heterologous protein as a secreted form. We adopted agarase signal peptide (ASP; 23 amino acid residues) from Agarivorans albus YKW-34 which is one of marine bacteia. When we used ASP to express $\beta$-agarase, about 42% activity was detected in media.

• Proceedings of the Korean Society of Toxicology Conference
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• 2001.10a
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• pp.111-111
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• 2001

The relationship between selenium and signal molecules is not well elucidated yet. It was found that physiological concentration of selenite, less than 3 $\mu$M, reduced ASKl activity and induced of PI3-Kinase/Akt pathways in HT1080 cells. Duration of these signal molecules by selenite was much longer than that by growth factors and other stresses. The longer duration time of these signal molecules may be important to maintain normal functions against stresses.(omitted)