Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effect of Agarase Signal Peptide from Agarivorans albus YKW-34 on Protein Secretion in Escherichia coli

대장균에서 단백질 분비에 대한 Agarivorans albus YKW-34의 Agarase 시그널펩티드의 효과

  • Lee, Joo-Young (Faculty of Marine Bioscience and Technology, Division of Marine Bioscience Engineering, Kangnung-Wonju National University) ;
  • Song, Dae-Geun (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute) ;
  • Son, Jin-Ki (Faculty of Marine Bioscience and Technology, Division of Marine Bioscience Engineering, Kangnung-Wonju National University) ;
  • Pan, Cheol-Ho (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
  • 이주영 (강릉원주대학교 해양생물공학과) ;
  • 송대근 (한국과학기술연구원 강릉분원 천연물소재센터) ;
  • 손진기 (강릉원주대학교 해양생물공학과) ;
  • 판철호 (한국과학기술연구원 강릉분원 천연물소재센터)
  • Received : 2010.01.13
  • Accepted : 2010.03.23
  • Published : 2010.06.30
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Abstract

To overcome the limitation of E. coli expression system such as inclusion body formation and disulfide bond failure, we tried to express the heterologous protein as a secreted form. We adopted agarase signal peptide (ASP; 23 amino acid residues) from Agarivorans albus YKW-34 which is one of marine bacteia. When we used ASP to express $\beta$-agarase, about 42% activity was detected in media.

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References

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