• Journal of Korean Biological Nursing Science
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• v.24 no.2
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• pp.95-103
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• 2022

Purpose: The purpose of this study was to provide multifaceted interventions for nurses and to confirm changes in their knowledge, perception, and actual practice on the adequate storage, conditions, and transportation of culture specimens. Methods: A one-group pretest-posttest experimental design was conducted with 41 nurses in two general wards of a tertiary acute care hospital in Seoul. Multifaceted interventions including education, feedback, posting guidelines and reminders, and improvement in specimen management accessibility were provided from May 2019 to January 2020. Outcomes were measured before and after the interventions. Knowledge and perception of the nurses were evaluated using self-reported questionnaires and actual practice by observation. Results: After the interventions, the average knowledge score on transportation time was significantly increased (Z= -4.89, p< .001). However, the knowledge score on storage methods was not significantly increased. The perception score was significantly increased (t= -3.19, p= .003). The proportion of specimen storage times, places, and conditions managed properly was significantly increased from 43.0% (46/107) to 77.1% (84/109) (p< .001). The average transportation time of blood samples to the laboratory significantly decreased from 3 hours 36 minutes (± 1 hour 52 minutes) to 3 hours 1 minute (± 1 hour 41 minutes) (t= 2.51, p= .013). The percentage of blood culture specimens arriving within 2 hours was increased significantly from 22.9% to 39.2% (χ²= 6.90, p= .009). Conclusion: The interventions were effective. However, some specimens remained in the ward longer than expected after the interventions. This requires further interventions.

• Applied Microscopy
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• v.43 no.1
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• pp.9-13
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• 2013

The helium ion microscope (HIM) has recently emerged as a novel tool for imaging and analysis. Based on a bright ion source and small probe, the HIM offers advantages over the conventional field emission scanning electron microscope. The key features of the HIM include (1) high resolution (ca. 0.25 nm), (2) great surface sensitivity, (3) great contrast, (4) large depth-of-field, (5) efficient charge control, (6) reduced specimen damage, and (7) nanomachining capability. Due to the charge neutralization by flood electron beam, there is no need for conductive metal coating for the observation of insulating biological specimens by HIM. There is growing evidence that the HIM has substantial potential for high-resolution imaging of uncoated insulating biological specimens at the nanoscale.

• Animal Systematics, Evolution and Diversity
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• v.33 no.4
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• pp.274-277
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• 2017

A sea star was collected from the Korea Strait in the waters adjacent to eastern Jeju Island, Korea ($33^{\circ}39^{\prime}86^{{\prime}{\prime}}N$, $127^{\circ}33^{\prime}12^{{\prime}{\prime}}E$) at a depth of 92 m on November 5, 2016. This specimen was identified as Marginaster paucispinus Fisher, 1913, from the family Poraniidae of the order Valvatida, based on morphological characteristics. The genus Marginaster Perrier, 1881 and M. paucispinus, which were first reported in the South China Sea, are new to the Korean fauna. Partial sequences of mitochondrial COI and 16S ribosomal RNA of M. paucispinus were have been determined for the first time and were deposited in GenBank. They are the first molecular records for the genus Marginaster.

• Journal of Species Research
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• v.7 no.4
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• pp.315-322
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• 2018

Based on morphological observation, we identified nine ciliates previously unrecorded in Korea, Euplotidium itoi Ito, 1958, Anteholosticha verrucosa (Foissner & Schade, 2000) Berger, 2008, Holostichides dumonti Foissner, 2000, Amphisiella australis Blatterer & Foissner, 1988, Terricirra matsusakai Berger & Foissner, 1989, Uroleptoides binucleatus binucleatus Hemberger, 1985, Uroleptoides magnigranulosus (Foissner, 1988) Berger, 2008, Leptopharynx costatus gonohymen Omar & Foissner, 2012, Pseudochilodonopsis mutabilis Foissner, 1981, from three sites in Korea, which were isolated from three environmental samples, consisting of one seawater and two soil samples. These ciliates were assigned to three classes, five orders, five families, and eight genera. In this study, we provide photographs and brief descriptions and remarks of the nine species.

• Journal of Species Research
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• v.2 no.1
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• pp.1-6
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• 2013

Conchodytes nipponensis (De Haan, 1844), a caridean shrimp living in the mantle cavity of a pen shell (Atrina pectinata) is reported for the first time from Korea. It was collected from a depth of 15 m depth. Its morphology is described and illustrated, and a color image of a living specimen is provided. This is at the same time the first record of the genus Conchodytes, and the first record of a bivalve mollucs associated shrimp, from Korea.

• Journal of Species Research
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• v.11 no.3
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• pp.174-179
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• 2022

In this study, we identified two marine synchaetid rotifers, Synchaeta grimpei Remane, 1929 and S. vorax Rousselet, 1902, in Korea, which are the first synchaetid rotifers collected from a marine environment in the country. Prior to this study, all six synchaetids recorded in Korea were collected from freshwater environments. The morphological characteristics of both species are consistent with those recorded in previous studies of each species. Synchaeta grimpei is distinguished from other synchaetid rotifers by its cone-shaped body, wide and flat apical field, indistinct auricles, and long foot with two separated small toes. The morphological characteristics of Korean S. vorax specimens were most similar to the original description of Rousselet (1902), with its slender and cylindrical trunk shape, strongly convex apical field, and short foot with two small, separated toes. The rami of the Korean S. vorax specimen contained one frontal hook and several distinct and large teeth. Here, we provide the morphological diagnoses of the two synchaetid rotifers and the sequences of the partial mitochondrial cytochrome c oxidase subunit I (COI) of the two species.

• Journal of Species Research
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• v.12 no.4
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• pp.341-354
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• 2023

This is the third series of catalogs reporting on Korean species discovered through the 'Discovery of Korean Indigenous Species'. This catalog includes 22 species of invertebrates, excluding insects. The catalog includes the scientific name, an abridged list of synonyms, collection sites, distribution, diagnosis, and figures for each species. Additionally, we provide the newly assigned Korean name, specimen voucher, and, if available, mitochondrial CO1 or 16S gene sequences of the species listed. All species identified and documented here will be officially listed on the 'National Species List of Korea', a database maintained by the National Institute of Biological Resources(NIBR).

• Proceedings of the KOSOMBE Conference
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• v.1997 no.05
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• pp.35-38
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• 1997

The interfacial bonding energy between laser surface treated HA layer and Ti alloy substrate was investigated using a mechanical push-out tester. The initial slope of shear-stress and reduced displacement curves, maximum interfacial bond strength and bonding energy were calculated from results of the push-out test. The calculated initial slpoes are 38 MPa for the Ti alloy(A), 65 MPa for the sandblast finished specimen(B), 95 MPa for the HA plasma spray coated specimen and 49 MPa for the laser surface treated specimen(D). The maximum interfacial bonding strength are 3 MPa for the A, 19 MPa for the B, 20 MPa for the C, 10 MPa for the D. The interfacial bonding energies are $3.3\times10^{-9}J/mm^2$ for the A, $15.5\times10^{-9}J/mm^2$ for the B, $15.6\times10^{-9}J/mm^2$ for the C and $18.3\times10^{-9}J/mm^2$ for the D. Microscopic observation shows that the breaking of the laser treated specimen had been occured through the boundary between HA layer and polymer resin, but the untreated specimen had been occured through the inside of HA coating layer.

• Animal Systematics, Evolution and Diversity
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• v.29 no.4
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• pp.288-293
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• 2013

A single specimen of the genus Sillago, collected from Gwangyang, Korea, in May 2009, is characterized by XI first dorsal fin spines, 3 or 4 rows of melanophore pattern along the second dorsal fin membrane, and a darkish posterior margin of the caudal fin. Our specimen was identified as Sillago sinica reported as a new species; this identification is confirmed by mitochondrial DNA cytochrome oxidase subunit I sequences, which show that our specimen corresponds to S. sinica (d=0.000) and differs from the congeneric species Sillago parvisquamis (d=0.170). Comparisons of Korean specimens previously reported as S. parvisquamis with specimens of S. sinica show that the S. parvisquamis specimens are actually S. sinica. We propose the new Korean name "buk-bang-jeom-bo-ri-myeol" for S. sinica.

• Biomedical Science Letters
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• v.13 no.4
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• pp.361-368
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• 2007

Biological samples can be fixed either by chemical method by using chemical solution or physical methods by using heat treatment. The problem in traditional heat fixation is unsatisfactory quality due to uneven heat conduction in specimen and loss of inner cell contents. Chemical fixation method also bears several intrinsic problems like the limit in specimen size, time consumption in fixative impregnation, and loss of low molecular weight cell components. These factors deteriorate the quality of fixed specimen, thus limit the magnification and contrast of tissue pictures. Microwave heat has been reported to be a good alternative to current chemical methods to overcome these problem. In this study, we tried to introduce the microwave energy method to routine fixation work in hospital. We replaced chemical fixative with saline to provide moderate reaction condition, and used frozen section to reduce time for sample preparation. Temperature was measured at each experiment. The fixation of rat kidney tissue with 2.45 GHz electromagnetic wave and saline showed similar result to the control group fixed with traditional chemical method. Human tumor tissue fixed with 2.45 GHz electromagnetic in frozen section was improved in terms of histochemistry of PAS and immunohistochemistry of tumor marker like cytokeratin. Total turnaround time was reduced from $24\sim38$ h to to $2\sim4$ h. In conclusion, the quality of samples prepared by microwave heating method was at least as good as that of traditional method. If the condition for the fixation of different specimens is standardized, this new method could be applied to routine work in hospital, and could save working time as well.