• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2003년도 춘계학술대회 논문집
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• pp.705-708
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• 2003

To observe a hydrated biological specimen, an environmental chamber was necessary to keep the specimen in a wet state under vacuum surroundings. The specimen holder is as follows designed consequently. The specimen holder consisted of two Si wafers, the centers of which were Si$_3$N$_4$(100nm thickness) windows of a 0.3mm square. The windows were made by a photo-lithographic method. The transmission of a window at 400eV is about 70%. A hydrated biological specimen was put between the two windows. When the chamber was closed, two wafers were contact at the metal mesh by the pressure of O-rings, and the specimen holder moved by the three micrometers.

• Journal of Species Research
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• 제9권4호
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• pp.375-376
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• 2020

A type specimen is the specimen originally used to name a new species; thus, it is one of the most significant materials for taxonomic study. In our study on the inventory and management of endemic species in Korea, we checked the type materials for Pseudolysimachion pusanensis (Y. Lee) Y. Lee, combination name Veronica pusanensis Y. Lee, recorded as deposited at the herbaria of the Ewha Womans University and the National Institute of Biological Resources, and failed to find any specimens. Thus, we concluded that all type specimens of V. pusanensis have since been misplaced. According to articles 9.11 and 9.13 of the International Code of Nomenclature for algae, fungi, and plants, it is necessary to select a neotype if the holotype is missing and no other original material exists. Therefore, we designate the neotype maintained in the herbarium of the National Institute of Biological Resources of the Republic of Korea.

• Mycobiology
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• 제49권5호
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• pp.461-468
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• 2021

The genus Laccaria (Hydnangiaceae, Agaricales) plays an important role in forest ecosystems as an ectomycorrhizal fungus, contributing to nutrient cycles through symbiosis with many types of trees. Though understanding Laccaria diversity and distribution patterns, as well as its association with host plants, is fundamental to constructing a balanced plant diversity and conducting effective forest management, previous studies have not been effective in accurately investigating, as they relied heavily on specimen collection alone. To investigate the true diversity and distribution pattern of Laccaria species and determine their host types, we used four different approaches: specimen-based analysis, open database search (ODS), NGS analysis, and species-specific PCR (SSP). As a result, 14 Laccaria species have been confirmed in Korea. Results regarding the species distribution pattern were different between specimen-based analysis and SSP. However, when both were integrated, the exact distribution pattern of each Laccaria species was determined. In addition, the SSP revealed that many Laccaria species have a wide range of host types. This study shows that using these four different approaches is useful in determining the diversity, distribution, and host of ECM fungi. Furthermore, results obtained for Laccaria will serve as a baseline to help understand the role of ECM fungi in forest management in response to climate change.

• 한국어류학회지
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• 제26권4호
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• pp.331-335
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• 2014

우리나라 강릉 연안에서 채집된 벼슬베도라치속 어류 1개체(체장 52.2 mm SL)의 표본을 근거로 Alectrias alectrolophus를 한국미기록종으로 기재하였다. 본 종은 등지느러미 기조수 LXIII, 뒷지느러미 기조수 I, 44, 척추골수 68이고, 가늘고 긴 체형을 가진다는 점에서 동속의 다른 종과 쉽게 구별된다. 본 종의 신한국명으로 "가는벼슬베도라치"를 제안한다.

• Journal of Species Research
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• 제12권2호
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• pp.154-157
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• 2023

A specimen of Henricia was collected from the adjacent waters of Moseulpo, Jeju Island, Korea, using fishing nets. The specimen was identified as Henricia aspera Fisher, 1906, which belongs to the family Echinasteridae of the order Spinulosida. This species can be distinguished from other Henricia species by its broad arms (R/r=4.1), swollen arm base, three to seven short abactinal spines, and close-meshed formation of the actinal skeleton. This study has newly recorded H. aspera inhabiting the Korean fauna, bringing the total number of known Henricia species in Korea to 16. This study provides a description of the morphological characteristics of H. aspera with photographs of each part. Additionally, a table is presented that compares the morphological characteristics of H. aspera with closely related Henricia species.

• 한국어류학회지
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• 제36권3호
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• pp.282-287
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• 2024

2021년 6월 경남 통영시 좌사리도의 암초 지역에서 채집된 나비고기과 유어 1개체(표준체장 28.6 mm)를 근거로 Chaetodon selene를 한국첫기록종으로 보고한다. 제주도 연안에서 수중사진만으로 소개된 바 있지만, 본 연구에서 확증표본을 근거로 그 형태적 특징을 상세하게 기술하였다. 신한국명으로 '노랑점나비고기'를 채택했으며, 등지느러미 극조가 12개이고, 몸의 후반부에 흑색 초승달 무늬와 눈을 가로지르는 흑색 세로줄무늬가 있는 것이 특징이다.

• 전자공학회논문지 IE
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• 제47권2호
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• pp.8-12
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• 2010

TEM(Transmission Electrion microsopy) 투과전자현미경은 재료의 기초 구조 분석과 반도체 또는 생물시편의 미세 구조분석에 널리 사용되는 장비이다. TEM 분석은 필수적으로 목적에 부합되는 적절한 시편제작이 수반되어야 한다. 다양한 전자 현미경 시편 제작 방법 중 본 논문에서는 FIB(Focus Ion Beam)를 이용한 시편 제작법 중 시편에 입사되는 에너지와 이온 Gun과 시편과의 상호 각도, 이온 밀링 깊이 조절 등의 실험을 통하여 표면 손상 최소화를 벌크 웨이퍼와 패턴화된 시편에서 실험하였다. 최소화된 표면 영향성(약 5nm)을 패턴화된 시편에 구현하였다.

• Journal of Preventive Medicine and Public Health
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• 제36권3호
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• pp.209-212
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• 2003

Because of advances of technologies in the field of genmic epidemiology in the recent years, specimen collection, storage and analysis became an essential part of research methodologies. DNA is now being used in epidemiologic studies to evaluate genetic risk factors and specimens other than the fresh whole blood can De used for PCR. Therefore, All nucleated cells, such as buccal swabs and urine specimens, are suitable for DNA analysis. For an unlimited source of genomic DNA, EBV transformation of lymphocytes can be used for immortalization. However, the type of specimen collected in genomic epidemiologic studies will depend on the study where the epidemiologist play a leading role for the design. We also briefly described various finds of analysis for SNP that is an essential part of the genomic epidemiology.

• Journal of Biosystems Engineering
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• 제24권3호
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• pp.243-252
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• 1999

The damage caused in the processes and distributions after harvesting the fruits and vegetables is attributed to the mechanical factors such as compressive and impact forces. Compression tests of biological materials provide an objective method for determining the apparent modulus of elasticity and mechanical properties which are significant in quality evaluation and control, and them maximum allowable compressive forces for minimizing mechanical damage. This study was performed to determine the mechanical properties of apple and pear, and to investigate effect of specimen shapes on the mechanical properties of them. A computer program was developed for measuring the mechanical properties and analyzing the data obtained from the measurement. Compression tests on the sample were performed with then replications at each treatment and at 25 mm/min loading rate. Mechanical properties of the apple was generally shown the higher value than those of the pear, and it was though that data obtained form the cylindrical specimen removed from the sample was more reliable than from the specimen cut in half.

• 환경생물
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• 제39권3호
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• pp.289-292
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• 2021

Henricia specimen was collected from the East Sea of Korea using fishing nets. The specimen was identified as Henricia hayashii (Djakonov 1961), belonging to the family Echinasteridae of the order Spinulosida. This species can be distinguished from other Henricia species by slender arms (R/r=7.4), constricted arm base, six to thirty-five slender spinelets, open-meshed, reticulated abactinal skeleton comprising semi-triangular, rod-like form, and small ossicles present inside the papular areas. This species superficially resembles H. reniossa in terms of its body size but differs in the shape and arrangement of the abactinal and actinal plates. To date, two genera of Echinasteridae, Aleutihenricia and Henricia, including a total of 14 species, have been reported in Korea. Herein, the morphological characteristics of H. hayashii are described, and photographs are provided.