• Title/Summary/Keyword: Biological safety

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Clean techniques for trace metal analysis in natural waters (자연수 중의 미량금속 분석을 위한 청결기술)

  • Kim, Kyung-Tae;Kim, Eun-Soo;Cho, Sung-Rok;Park, Jun-Kun;Moon, Deok-Soo;Kim, Hyeon-Ju
    • Proceedings of KOSOMES biannual meeting
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    • 2007.11a
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    • pp.43-49
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    • 2007
  • The metals we are familiar with(Al, Ag, Au, Cu, Cd, Co, Fe, Ni, Pb, Zn, etc) are common elements and conservative pollutants. Although metals are often vital constituents of the metabolism of living organisms(trace elements}, a number of them are toxic if their concentration exceeds a certain threshold. It has long been recognized that measurements of trace metals in natural waters are often subject to large errors in terms of precision and accuracy. Since 1975 in US and European countries, seawater concentration of many trace metals have been shown to be factors of 10-1,000 lower than those previously accepted. Vertical profiles have been found to be consistent with known biological, physical and/or geochemical processes. These are resulted from major advances in analytical instrumentation and methodology for trace elements, and greater attention has been given to assuring the elimination of contamination during sampling, storage, and analysis.

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A Study of Computerized Industrial Hygiene Control System (작업환경관리 전산화에 관한 연구)

  • Lee, Song Kwon;Lee, Young Sei;Kim, Young Su;Kim, Yong Lae
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.4 no.1
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    • pp.117-126
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    • 1994
  • Some of the important and vital roles performed by the Health Care Center of Pohang Iron & Steel Co., Ltd.(POSCO) are the periodic monitoring of the work sites as well as regular medical check-ups for each workers to ensure both pleasant and safe working conditions and to protect employees' health. Pohang Steel Works, which has 42 plants, 26 facilities and 12,000 employees, has an annual crude steel production capacity of 9,670,000 using 24,500,000 ton of raw materials. We have measured lots of areal and personal working environment according to Occupational Safety and Health Law semiannually. All data collected on industrial hygiene is stored and managed by personal computer. The developed of a computerized industrial hygiene control system linked to the company's main computer system will provide efficient evaluation of the working environment as well as critical informations on employees' health. Development of this system had begun in May, 1992 and was completed in March, 1994. The computerized industrial hygiene control system can; 1. store industrial hygiene data, providing key pertinent to the diagnosis of occupational related illnesses, and if necessary, the basis for improving the working environment, 2. decide type and time of medical examination of each work for exposure to specific agents, and supply proper protection periodically, 3. prevent occupational related illnesses through evaluation of environmental and biological monitoring, and performance of epidemiological surveillance, 4. facilitate proper job placement through linking of industrial hygiene control and personnel information systems, 5. monitor job processes. levels of chemical agents used, and new agents introduced, 6. and increase productivity by saving time resulting from the implementation of the computerized sysyte,

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Suppression of Akt-HIF-1α signaling axis by diacetyl atractylodiol inhibits hypoxia-induced angiogenesis

  • Choi, Sik-Won;Lee, Kwang-Sik;Lee, Jin Hwan;Kang, Hyeon Jung;Lee, Mi Ja;Kim, Hyun Young;Park, Kie-In;Kim, Sun-Lim;Shin, Hye Kyoung;Seo, Woo Duck
    • BMB Reports
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    • v.49 no.9
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    • pp.508-513
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    • 2016
  • Hypoxia-inducible factor (HIF)-1α is a key regulator associated with tumorigenesis, angiogenesis, and metastasis. HIF-1α regulation under hypoxia has been highlighted as a promising therapeutic target in angiogenesis-related diseases. Here, we demonstrate that diacetyl atractylodiol (DAA) from Atractylodes japonica (A. japonica) is a potent HIF-1α inhibitor that inhibits the Akt signaling pathway. DAA dose-dependently inhibited hypoxia-induced HIF-1α and downregulated Akt signaling without affecting the stability of HIF-1α protein. Furthermore, DAA prevented hypoxia-mediated angiogenesis based on in vitro tube formation and in vivo chorioallantoic membrane (CAM) assays. Therefore, DAA might be useful for treatment of hypoxia-related tumorigenesis, including angiogenesis.

An Estimation on the Field Application of Consolidants According to Rock Quality (암석 재질에 따른 보존처리제의 현장 적용성 평가)

  • Kim, Jae-Hwan;Han, Min-Su;Lee, Jang-Jon;Song, Chi-Young;Lee, Jae-Man;Kim, Min-Ji;Lee, Myeong-Sung
    • 보존과학연구
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    • s.30
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    • pp.79-91
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    • 2009
  • Stone cultural heritages are mostly situated in field, so they are damaged from mechanical, chemical and biological weathering, and their degree of strength is also weaken. Therefore, the scientific conservation of the stone cultural heritages are necessary in order to the long-term maintenance and safety conservation. In this study, we attempt to estimate on the field application of consolidants which are Wacker OH 100, Remmers KSE 300 and 1T1G according to the quality of the rocks. Based on the ultrasonic velocity, conditions of weathering damage before treatment are mainly ranked completely weathering condition (CW) or partly highly weathering condition (HW). After the first treatment, weathering damage conditions are changed by the high weathering condition (HW). These results shows that the internal pores of stone are filled with consolidant materials, so average ultrasonic velocities increases after treatment. And Remmers KSE 300 which is consolidant material is highest rate of increase of average ultrasonic velocities.

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Engineering Properties of Sewage Polymer Concrete Culvert (폴리머 콘크리트를 적용한 하수암거의 공학적 특성)

  • Kwon, Seung Jun;Min, Byung Yoon;Park, Sang Soon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.16 no.6
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    • pp.9-17
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    • 2012
  • Concrete sewage culvert shows degradation with time since it is always exposed to various harmful ions, and deterioration of concrete culvert propagates to structural safety problems. After reclamation, maintenance for concrete sewage culvert is very difficult so that high durable and structural performance are essential for the sewage concrete culvert. Recently polymer concrete has been used to improve mechanical properties and durability performance. In this paper, engineering properties are evaluated for sewage culvert made with polymer concrete, and leakage and adhesive strength between joints are evaluated with small-scale models. The polymer sewage culvert shows high compressive strength over 100MPa with low water permeability and chloride penetration. Furthermore, high resistances to chemical and biological attack are evaluated. Through tests for leakage and adhesive, unification of joints is verified with evaluation of no leakage and high adhesive strength. Precast polymer sewage culvert in this paper can be actively used for severe conditions like sewage lines.

A Study on the Planning of Civil Defense Shelter and Design 1 - On the ideas of the State of Civil Defense Shelter & Design Criteria - (민방위 대피시설 계획 및 설계 방안에 관한 연구 1 - 민방위 대피시설의 현황 및 설계기준을 중심으로 -)

  • Park, Namkwun;Paik, Sungkun
    • Journal of the Society of Disaster Information
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    • v.10 no.3
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    • pp.358-365
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    • 2014
  • North Korea has been preparing for WMD(Weapons Mass Destruction) using asymmetric force since it recognized the economic effects of CBR (Chemical, Biological, Radiological) weapons system operation and the limitations of conventional weapons. However, the threat only to conventional bombs, missiles and etc. is considered on the current Civil Defense Shelter, which could increase civil damages, not responding appropriately to disasters such as CBR weapons, terror attacks and etc. Therefore, this study confirms the current situation of Civil Defense Shelter and design criteria focused on CBR disasters so that we could make a plan and design of Civil Defense Shelter. In addition, we suggest the research result and improvements on Civil Defense Shelter and Design.

Development of Autonomous Sprayer Considering Tracking Performance on Geometrical Complexity of Ground in Greenhouse

  • Lee, Dong Hoon;Lee, Kyou Seung;Cho, Yong Jin;Lee, Je Yong;Chung, Sun-Ok
    • Journal of Biosystems Engineering
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    • v.37 no.5
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    • pp.287-295
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    • 2012
  • Purpose: Some of the most representative approaches are to apply next generation technologies to save energy consumption, fully automated control system to appropriately maintain environmental conditions, and autonomous assistance system to reduce labor load and ensure operator's safety. Nevertheless, improvement of upcoming method for soil cultured greenhouse has not been sufficiently achieved. Geometrical complexity of ground in protected crop cultivation might be one of the most dominant factors in design of autonomous vehicle. While there is a practical solution fairly enough to promise an accurate travelling, such as autonomous sprayer guided by rail or induction coil, for various reasons including the limitation of producer's budget, the previously developed sprayer has not been widely distributed to market. Methods: In this study, we developed an autonomous sprayer considering travelling performance on geometrical complexity of ground in soil cultured greenhouse. To maintain a stable travelling and to acquire a real time feedback, common wire with 80 mm thick and body frame and sprayer boom. To evaluate performance of the prototype, tracking performance, climbing performance and spraying boom's uniform leveling performance were individually evaluated by corresponding experimental tests. Results: The autonomous guidance system was proved to be sufficiently suitable for accurate linear traveling with RMS as lower than approximately 10 cm from designated path. Also the prototype could climb $10^{\circ}$ of ground's slope angle with 40 kg of water weight. Uniform leveling of spraying boom was successfully performed within $0.5^{\circ}$ of sprayer boom's slope. Conclusions: Considering more complex pathways and coarse ground conditions, evaluations and improvements of the prototype should be performed for promising reliability to commercialization.

Validation of urinary N-methylacetamide reference material for proficiency test (숙련도시험을 위한 소변 중 N-메틸아세트아미드 표준시료 검증)

  • Lee, Mi-Young
    • Analytical Science and Technology
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    • v.27 no.6
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    • pp.339-346
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    • 2014
  • The author assessed the availability of urine reference material for proficiency test provided for laboratories in occupational health. N-methylacetamide is the biomarker of exposure to N,N-dimethyl acetamide, which was used as the substitute for hepatotoxic N,N-dimethylformamide (DMF). N-methylacetamide (NMAC) urine samples of 3 different levels covering the 0.2~2 times of the exposure limit were tested. Stability test up to 180 days (0, 7, 30, 60, 180 days) at 4 different temperatures (-60, -20, 5, $25^{\circ}C$) and homogeneity test were performed for these samples. New analytical condition by GC/MSD using SIM mode (m/z 58, 59) and DB-624 column was investigated for better selectivity, sensitivity and resolution. Urinary NMAC samples showed good homogeneity for 3 levels. These samples also showed good stability up to 180 days. The data of stability and homogeneity of urinary DMAC confirmed the basis of including this item into Korean proficiency test for occupational health laboratories since 2008.

Antagonistic Activities of Bacillus spp. Strains Isolated from Tidal Flat Sediment Towards Anthracnose Pathogens Colletotrichum acutatum and C. gloeosporioides in South Korea

  • Han, Joon-Hee;Shim, Hongsik;Shin, Jong-Hwan;Kim, Kyoung Su
    • The Plant Pathology Journal
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    • v.31 no.2
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    • pp.165-175
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    • 2015
  • Anthracnose is a fungal disease caused by Colletotrichum species that is detrimental to numerous plant species. Anthracnose control with fungicides has both human health and environmental safety implications. Despite increasing public concerns, fungicide use will continue in the absence of viable alternatives. There have been relatively less efforts to search antagonistic bacteria from mudflats harboring microbial diversity. A total of 420 bacterial strains were isolated from mudflats near the western sea of South Korea. Five bacterial strains, LB01, LB14, HM03, HM17, and LB15, were characterized as having antifungal properties in the presence of C. acutatum and C. gloeosporioides. The three Bacillus atrophaeus strains, LB14, HM03, and HM17, produced large quantities of chitinase and protease enzymes, whereas the B. amyloliquefaciens strain LB01 produced protease and cellulase enzymes. Two important antagonistic traits, siderophore production and solubilization of insoluble phosphate, were observed in the three B. atrophaeus strains. Analyses of disease suppression revealed that LB14 was most effective for suppressing the incidence of anthracnose symptoms on pepper fruits. LB14 produced antagonistic compounds and suppressed conidial germination of C. acutatum and C. gloeosporioides. The results from the present study will provide a basis for developing a reliable alternative to fungicides for anthracnose control.

Biological Characteristics of New Paddy Field Herbicide Metazosulfuron Granule for Paddy Weeds (신규 수도용 제초제 Metazosulfuron입제의 생물특성)

  • Lee, In-Yong;Kim, Chang-Seok;Lee, Jeong-Ran;Moon, Byung-Chul;Lee, Chang-Gyu
    • Korean Journal of Weed Science
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    • v.31 no.3
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    • pp.308-312
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    • 2011
  • A new paddy field herbicide, metazosulfuron granule is an herbicide for controlling annual and perennial weeds occurring in machinery transplanting paddy field. It is recommended to apply $30kg\;ha^{-1}$ 15 days after transplanting. As a result of preliminary tests, it could control seven annual species such as Echinochloa crus-galli, Bidens tripartita, Ludwigia prostrata, Monochoria vaginalis var. plantaginea, etc. and four perennial species including Cyperus serotinus, Sagittaria trifolia, Eleocharis kuroguwai, and Scirpus juncoides. Monochoria vaginalis var. plantaginea, S. trifolia and S. juncoides resistant to sulfonylurea- herbicides were also controlled. Because application timing of the herbicide is wide, it is very efficient to control the spraying timing by users. Phytotoxicity of rice cultivars was not observed, and the impact on the environment is very limited.