• Journal of Environmental Science International
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• v.4 no.5
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• pp.501-508
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• 1995

Reuse of industrial effluents through the cooling systems in a petrochemical complex was described. The partial oxidation of the effluents from the biological treatment plant was examined, using Fenton's reagent as a pretreatment step prior to a next treatment of the effluents. Next tertiary treatment using fixed-film reactor resulted in marked reductions in COD and suspended solids. The continuous fixed-film process with Fenton oxidation pretreatment showed a 23% increase in the COD removal efficiency when compared to that without pretreatment of Fenton oxidation under the volumetric organic loading rate of 0.1 kg COD/m3/day. The Fenton oxidation treatment seemed to be a possible method for tertiary biological treatment to reduce the residual toxicity with the enhanced biodegradation of the effluents.

• Environmental Engineering Research
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• v.15 no.1
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• pp.3-7
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• 2010

To better understand the ecology of tetrade forming organisms (TFOs) floating in a large amount of dairy wastewater treatment plant (WWTP) effluent (sequencing batch reactor [SBR]) during the inefficient phosphorus (P) removal process of an enhanced biological P removal system, the TFOs from the effluent of a full scale WWTP were separated and attempts made to culture the TFOs in presence/absence of oxygen. The intact TFOs only grew aerobically in the form of unicellular short-rods. Furthermore, to identify the intact TFOs and unicellular short-rods the DNAs of both were extracted, analyzed using their denaturing gradient gel electrophoresis (DGGE)-profiles and then sequenced. The TFOs and unicellular short-rods exhibited the same banding pattern in their DGGE-profiles, and those sequencing data resulted in their identification as Acinetobacter sp. The intact TFOs appeared in clumps and packages of tetrade cells, and were identified as Acinetobacter sp., which are known as strict aerobes and efficient P-removers. The thick layer of extracellular polymeric substance surrounding Acinetobacter sp. may inhibit phosphate uptake, and the cell morphology of TFOs might subsequently be connected with their survival strategy under the anaerobic regime of the SBR system.

• KSBB Journal
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• v.14 no.1
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• pp.119-123
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• 1999

A jet-loop reactor was used for the biological treatment of ethylene glycol(EG) which is a main component of polyester weight-loss wastewater, and is difficult to be removed by physicochemical treatments. Volumetric oxygen coefficient(kLa) of jet-loop reactor was significantly larfgeer that of air-lift reactor. When organic loading rates of synthetic polyester weight-loss wastewater were 2.64 $kgOD_{Mn}/m^3$.day and 3.07 $kgCOD_{Cr}/m^3$.day, the effluent concentrations were measured as 154 $mgCOD_{Mn}/L$ and 156$mgCOD_{Cr}/L$, and removal efficiencies were found as 93%and 93.6%, respectively. The specific removal rate was proportionally increased from 0.25 to 1.60 $kgCOD_{Mn}$-removed/kgMLVSS.day as specific loading rate was increased from 0.25 to 1.72 $kgCOD_{Mn}$/kgMLVSS.day. Also, kinetics constants such as $K_s$, k, $K_d$, and Y were estimated as 89 mg/L, $0.05 hr^{-1}$, 0.1$day^{-1}$ and 0.78 respectively. When the organic loading rates of real polyester weight-loss wastewater were 2.64 $kgOD_{Mn}/m^3$. and 5.24 $kgCOD_{Cr}/m^3$. day, the effluent concentrations were measured as 150 $mgCOD_{Mn}$/L, and 306 $mgCOD_{Cr}$/L, and removal efficiencies were found as 93.2% and 93%, respectively. This study demonstrated that EG in the wastewater could be efficiently removed biologically using a jet-loop reactor.

• Journal of Environmental Science International
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• v.8 no.1
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• pp.107-113
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• 1999

A new biological nutrient removal system combining $A^2/O$ process with fixed film was developed in this work and the characteristics of denitrification were especially investigated in the combined fixed film reactor(CFFR). Media was added in the anaerobic, anoxic and aerobic reactors, respectively. Tests were made to establish the effluent level of $NO_x-N$, COD, DO and nitrite effects on $NO_x-N$ removal in the CFFR by decreasing hydraulic retention time (HRT) from 10.0 to 3.5 hours and by increasing internal recycle ratio form 0% to 200%. The influent was synthesized to levels similar to the average influent of municipal wastewater treatment plants in Korea. SARAN media with a porosity of 96.3% was packed 40% / 130% / 25% based on its reactor volume, respectively. It was found that COD rarely limited dentrification in the anoxic reactor because of high $C/NO_x/-N$ ratio in the anoxic reactor, while DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent inhibited denitrification in the anoxic reactor. It was proved that the critical points of DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent were 0.15mg/L and 10%, respectively. As the internal recycle ratio increased, DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent increased. Especially, at the condition of internal recycle ratio, 200%, DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent exceeded the critical points of 0.15mg/L and 10%, respectively. Then, denitrification efficiency considerably decreased. Consequently, it was represented that the control of DO concentration in the anoxic reactor and $NO_2-N/NO_x/-N$ from the aerobic effluent can assure effective denitrification.

• Journal of Environmental Science International
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• v.11 no.6
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• pp.569-576
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• 2002

Anaerobic/aerobic reactor system was used to treat a synthetic wastewater with glucose as carbon sources(0.38~2.29 kg COD/m3.day) and Acid Red 14(1.05 "24.00 g Acid Red 141m3.day, color degree of 570 ~ 1710). COD removal efficiency by the anaerobic stage in operation period were above 90 % organic loading rate of 0.38 ~ 2.29 kg COD/m3.day(except, adaptation period) and the removal efficiency of the whole system were above 96 %. The decolorization of the Acid Red 14 was through the alteration of the dye structure(or cleavage of the Azo bond) during the anaerobic treatment. In the A/A system, the anaerobic stage played an essential role in removing both color and COD. In addition it also improves biodegradability of dye f3r further aerobic treatment. After operation, average MLSS concentration of anaerobic sludge reactor, anaerobic fixed-bed reactor and aerobic fixed-bed reactor were 17100mg/L, 20000mg/L, and 10000mg/L, respectively.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.6
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• pp.677-686
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• 2006

This study was the effectiveness of two downflow BAF(Biological Aerated Filter) systems at conventional water treatment system. A BAF reactor placed in front of coagulation and sedimentation tanks(Mode A) and after coagulation and sedimentation tanks(Mode B) that were compared in terms of removal of suspended particles, organic matters, and ammonia nitrogen. The suspended particles removal efficiency was over 80% for both Mode A and B, although Mode A gave slightly better results. $BOD_5$ removal and nitrification efficiencies were more than 90% for both reactor. The organic matter and ammonia removals were also superior in the Mode A. The biofilm thickness and biomass increased as increment of EBCT and the upper part of reactor more about 30% than lower part. The specific oxygen uptake rate(SOUR) was higher the upper part of reactor and Mode A than the lower part of reactor and Mode B. A cost analysis showed that the Mode A system was more cost effectiveness. It could save the coagulant dose by about 67% and the chlorine demand by about 95%. The ideal place to put the BAF reactor was in front of the coagulation/sedimentation process.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.7
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• pp.752-756
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• 2006

The independent anoxic reactor was introduced in biological aerated filters as the regulation of water quality requirement, especially total nitrogen, had been strengthened. The process studied in this work was upflow $Biobead^{(R)}$ process which was used commercial invented for removal of organic materials and nitrification. For the purpose of evaluating the independent anoxic reactor, PCR-DGGE, of the molecular biological methods, was performed. Two types of nitrite reductase genes were selected. One is nirS represented cytocrome $cd_1$ nitrite reductase gene and the other is nirK represented Cu-containing nitrite reductase gene. Denitrifier community in the independent anoxic reactor was analyzed with PCR-DGGE using these two denitrifying functional genes. As the result of the PCR, only nirS gene was detected between nirS and nirK. With the result of the DGGE, specific bands became strong, as the operating days were longer, nitrate loading rate was increased. otherwise those of the initial activated sludge showed various bands. In the consequence of the sequence of DGGE bands, various denitrifiers were sequenced in the initial activated sludge, while specific denitrifiers like alcaligenes faecalis were predominant in the anoxic reactor. Consequently, introduction of the independent anoxic reactor made it possible to achieve 96% denitrification efficiency, and was proper for the modification of BAF process.

• Journal of Microbiology and Biotechnology
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• v.13 no.3
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• pp.385-393
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• 2003

Microbial communities were analyzed in an anaerobic/aerobic sequencing batch reactor (SBR) fed with glucose as a sole carbon source. Scanning electron microscopy (SEM) showed that tetrad or cuboidal packet bacteria dominated the microbial sludge. Quinone, slot hybridization, and 165 rRNA gene sequencing analyses showed that the Proteobacteria beta subclass and the Actinobacteria group were the main microbial species in the SBR sludge. However, according to transmission electron microscopy (TEM), the packet bacteria did not contain polyphosphate granules or glycogen inclusions, but only separate coccus-shaped bacteria contained these, suggesting that coccus-shaped bacteria accumulated polyphosphate directly and the packet bacteria played other role in the enhanced biological phosphorus removal (EBPR). Based on previous reports, the Actinobacteria group and the Proteobacteria beta subclass were very likely responsible for acid formation and polyphosphate accumulation, respectively, and their cooperation achieved the EBPR in the SBR operation which was supplied with glucose.

• Environmental Engineering Research
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• v.11 no.2
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• pp.63-76
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• 2006

Multivariate analysis and batch monitoring on a pilot-scale sequencing batch reactor (SBR) are described for integrated wastewater treatment management system, where a batchwise multiway independent component analysis method (MICA) are used to extract meaningful hidden information from non-Gaussian wastewater treatment data. Three-way batch data of SBR are unfolded batch-wisely, and then a non-Gaussian multivariate monitoring method is used to capture the non-Gaussian characteristics of normal batches in biological wastewater treatment plant. It is successfully applied to an 80L SBR for biological wastewater treatment, which is characterized by a variety of error sources with non-Gaussian characteristics. The batchwise multivariate monitoring results of a pilot-scale SBR for integrated wastewater treatment management system showed more powerful monitoring performance on a WWTP application than the conventional method since it can extract non-Gaussian source signals which are independent and cross-correlation of variables.

• Journal of Microbiology
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• v.43 no.5
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• pp.451-455
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• 2005

In this study, whole cells and a crude enzyme of Candida peltata were applied to an electrochemical bioreactor, in order to induce an increment of the reduction of xylose to xylitol. Neutral red was utilized as an electron mediator in the whole cell reactor, and a graphite-Mn(IV) electrode was used as a catalyst in the enzyme reactor in order to induce the electrochemical reduction of $NAD^+$ to NADH. The efficiency with which xylose was converted to xylitol in the electrochemical bioreactor was five times higher than that in the conventional bioreactor, when whole cells were employed as a biocatalyst. Meanwhile, the xylose to xylitol reduction efficiency in the enzyme reactor using the graphite-Mn (IV) electrode and $NAD^+$ was twice as high as that observed in the conventional bioreactor which utilized NADH as a reducing power. In order to use the graphite-Mn(IV) electrode as a catalyst for the reduction of $NAD^+$ to NADH, a bioelectrocatalyst was engineered, namely, oxidoreductase (e.g. xylose reductase). $NAD^+$ can function in this biotransformation procedure without any electron mediator or a second oxidoreductase for $NAD^+/NADH$ recycling