Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Microbial Communities of Activated Sludge Performing Enhanced Biological Phosphorus Removal in a Sequencing Batch Reactor Supplied with Glucose

  • Jeon, Che-Ok (Department of Microbilolgy, B57A Wing Hall, Wing Drive, Cornell University, Ithaca) ;
  • Seung, Han-Woo (School of Environmental Science & Engineering, Pohang University of Science and Technology) ;
  • Park, Jong-Moon (School of Environmental Science & Engineering, Pohang University of Science and Technology)
  • Published : 2003.06.01
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Abstract

Microbial communities were analyzed in an anaerobic/aerobic sequencing batch reactor (SBR) fed with glucose as a sole carbon source. Scanning electron microscopy (SEM) showed that tetrad or cuboidal packet bacteria dominated the microbial sludge. Quinone, slot hybridization, and 165 rRNA gene sequencing analyses showed that the Proteobacteria beta subclass and the Actinobacteria group were the main microbial species in the SBR sludge. However, according to transmission electron microscopy (TEM), the packet bacteria did not contain polyphosphate granules or glycogen inclusions, but only separate coccus-shaped bacteria contained these, suggesting that coccus-shaped bacteria accumulated polyphosphate directly and the packet bacteria played other role in the enhanced biological phosphorus removal (EBPR). Based on previous reports, the Actinobacteria group and the Proteobacteria beta subclass were very likely responsible for acid formation and polyphosphate accumulation, respectively, and their cooperation achieved the EBPR in the SBR operation which was supplied with glucose.

Keywords

References

  1. Microbiol. Rev. v.59 Phylogenetic identification and in situ detection of individual microbial cells without cultivation Amann,R.;I.W.Ludwig;K.H.Schleifer
  2. Standard Methods for the Examination of Water and Wastewater(19th ed.) American Public Health Association
  3. Water Res. v.6 Differences in polyphosphate accumulation and phosphate adsorption by Acinetobacter isolates from wastewater producing polyphosphate: AMP phosphotransferase Bark,K.;A.Sponner;P.Kampfer;S.Grund;W.Dott
  4. Appl. Environ. Microbiol v.62 Isolation of thermus strains from hot composts (60 to 80°C) Beffa,T.;M.Blanc;P.F.Lyon;G.Vogt;M.Marchiani;J.L.Fischer;M.Aragno
  5. Appl. Environ. Microbiol v.65 Identification of some of the major groups of bacteria in efficient and nonefficinet biological phosphorus removal activated sludge systems Bond,P.L.;R.Erhart;M.Wagner;J.Keller;L.L.Blackall
  6. Appl. Environ. Microbiol v.61 Bacterial community structures of phosphate-removing and non-phosphate removing activated sludges from sequencing batch reactors Bond,P.L.;P.Hugenholtz;J.Keller;L.L.Blackall
  7. Nucleic Acids Res. v.27 A new version of the RDP (Rivosomal Database Project) Bonnie,L.M.;J.R.Cole;C.T.Parker;J.G.M.Garrity;N.Larsen;B.Li;T.G.Lilburn;M.J.McCaughey;G.J.Olsen;R.Overbeek;S.Pramanik;T.M.Schimidt;J.M.Tiedje;C.R.Woese https://doi.org/10.1093/nar/27.1.171
  8. Environ. Technol. v.11 Glucose induced breakdown of enhanced biological phosphate removal Cech,J.S.;P.Hartman https://doi.org/10.1080/09593339009384907
  9. J. Bacteriol v.168 Isolation of intact chain of polyphosphate from Propionibacterium shermanii grown on glucose or lactate Clark,J.E.;H.Beegen;H.G.Wood
  10. Water Res. v.22 A combined membrane filter-immunofluorescent technique for the in situ identification and enumeration of Acinetobacter in activated sludge Cloete,T.E.;P.L.Steyn https://doi.org/10.1016/0043-1354(88)90142-X
  11. Appl. Environ. Microbiol v.66 Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantification Crocetti,G.R.;P.Hugenholtz;P.L.Bond;A.Schuler;J.Keller;D.Jenkins;L.L.Blackall https://doi.org/10.1128/AEM.66.3.1175-1182.2000
  12. Water Sci. Technol v.17 no.11;12 Some physiological characteristics of Acinetobacter spp. accumulating large amounts of phosphate Deinema,M.H.;M. van Loosdrecht;A.Scholten
  13. Microbiol. Ecol. v.2 Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater Fuhs,G.W;M.Chen https://doi.org/10.1007/BF02010434
  14. Appl. Environ. Microbiol v.64 Quinone profiling of bacterial communities in natural and synthetic activated sewage for enhanced phosphate removal Hiraishi,A.;Y.Ueda;J.Ishihara
  15. J. Gen. Appl. Microbiol v.45 Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection Hiraishi,A.;Y.Ueda;J.Ishihara;T.Mori
  16. Water Res. v.34 Enhanced biological phosphorus removal in a sequencing batch reactor supplied with glucose as a sole carbon source Jeon,C.O.;J.M.Park
  17. Water Res. v.25 The applied microbiology of enhanced biological phosphate removal; accomplishments and needs Jerkins,D.;V.Tandoi https://doi.org/10.1016/0043-1354(91)90177-R
  18. Microb. Ecol. v.32 Characterization of bacterial communities from activated sludge: Culture-dependent numerical identification versus in situ identification using group- and genus-specific rRNA-targeted oligonucleotide probes Kampfer,P.;R.Erhart;C.Beimfohr;J.Bohringer;M.Wagner;R.Amann
  19. Methods Enzymol v.53 Determination of contents and redox states of ubiquinone and menaquinone Kroger,A https://doi.org/10.1016/S0076-6879(78)53059-0
  20. J. Microbiol. Biotechnol v.11 Removal behavior of biological nitrogen and phosphorus, and prediction of microbial community composition with its function, in an anaerobic-anoxic system from weak sewage Lee,J.W.;E.S.Choi;K.I.Gil;H.W.Lee;S.H.Lee;S.Y.Lee;Y.K.Park
  21. Appl. Environ. Microbiol v.61 Taxon-specific probes for the cellulolytic genus Fibrobacter reveal abundant and novel equine-associated populations Lin,C.;D.A.Stahl
  22. Microbiology v.146 Microbial community changes in biological phosphate-removal systems on altering sludge phosphorus content Liu,W.T.;K.Linning;K.Nakamura;T.Mino;T.Matsuo;L.J.Forney
  23. Water Res. v.30 Glycogen accumulating population and its anaerobic substrate uptake in anaerobic-aerobic activated sludge without biological phosphorus removal Liu,W.T.;T.Mino;K.Nakamura;T.Matsuo
  24. Water S. A. v.12 A study of selected characteristics of Acinetobacter spp. isolated from activated sludge in anaerobic/anoxic/aerobic and aerobic systems Lotter,L.H.;M.C.Wentzel;R.E.Loewenthal;G.A.Ekama;G.R.Marais
  25. Int. J. Syst. Evol. Microbiol v.50 Three isolates of novel polyphosphate-accumulating Gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp. nov. and Tetrasphaera australiensis sp. nov. Maszenan,A.M.;R.J.Seviour;B.K.C.Patel;P.Schumann;J.Burghardt;Y.Tokiwa;H.M.Stratton https://doi.org/10.1099/00207713-50-2-593
  26. Int. J. Syst. Evol. Microbiol v.49 Friedmanniella spumicola sp. nov. and Friedmanniella capsulata sp. nov. from activated sludge foam: Grampositive cocci that grow in aggregates of repeating groups of cocci Maszenan,A.M.;R.J.Seviour;B.K.C.Patel;P.Schumann;J.Burghardt;R.I.Webb;J.A.Soddel;G.N.Rees https://doi.org/10.1099/00207713-49-4-1667
  27. Int. J. Syst. Evol. Microbiol v.49 Tessaracoccus bendigoensis gen. nov., sp. nov., a Gram-positive coccus in regular packages of tetrads, isolated from activated sludge biomass Maszenan,A.M.;R.J.Seviour;B.K.C.Patel;P.Schumann;G.N.Rees https://doi.org/10.1099/00207713-49-2-459
  28. Biological Phosphate Removal from Wastewaters Effect of phosphorus accumulation on acetate metabolism in the biological pholphorus removal process Mino,T.;V.Arun;Y.T.Suzuki;T.Matsuo
  29. Int. J. Syst. Bacteriol v.45 Microlunatus phosphovorus gen. nov., sp. nov., a new Gram-positive polyphosphate-accumulating bacterium isolated from activated sludge Nakamura,K.;A.Hiraishi;Y.Yoshimi;M.Kawaharasaki;K.Masuda;Y.Kamagata https://doi.org/10.1099/00207713-45-1-17
  30. J. Microbiol. Biotechnol v.12 Microbial community analysis of 5-stage biological nutrient removal process with step feed system Park,J.B.;H.W.Lee;S.Y.Lee;J.O.Lee;I.S.Bang;E.S.Choi;D.H.Park;Y.K.Park
  31. Appl. Microbiol. Biotechnol v.38 Production of poly-β-hydroxylate in Acinetobacter spp. isolated from activated sludge Rees,G.N.;G.Vasilidis;J.W.May;R.C.Bayly https://doi.org/10.1007/BF00167136
  32. Appl. Environ. Microbiol v.63 Group-specific small-subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems Reyes,F.L.;E.Los;W.Ritter;L.Raskin
  33. Mol. Biol. Evol. v.4 The neighbor-joining method: A new method for reconstructing phylogenetic trees Saitou,N.;M.Nei
  34. Int. J. Syst. Evol. Microbiol v.50 Micropruina glycogenica gen. nov., sp. nov., a new Gram-positive glycogen accumulating bacterium isolated from activated sludge Shintani,T.;W.T.Liu;S.Hanada;Y.Kamagata;S.Miyaoka;T.Suzuki;K.Nakamura https://doi.org/10.1099/00207713-50-1-201
  35. Appl. Environ. Microbiol v.63 Phylogenetic analysis and in situ identification of bacteria in activated sludge Snaidr,J.;R.Amann;I.Huber;W.Ludwig;K.H.Schleifer
  36. Appl. Environ. Microbiol v.54 Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology Stahl,D.A.;B.Flesher;H.R.Mansfield;L.Montgomery
  37. Water Sci. Technol v.38 no.8;9 Morphology, in-situ characterization with rRNA targeted probes and respiratory quinone profiles of enhanced biological phosphorus removal sludge Sudiana,I.M.;T.Mino;H.Satoh;T.Matsuo
  38. Nucleic Acids Res. v.22 Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight metrix choice Thompson,J.D.;D.G.Higgins;T.J.Gibson;W.Clustal https://doi.org/10.1093/nar/22.22.4673
  39. Antonie van Leewenhoek v.71 Metabolism of microorganisms responsible for enhanced biological phosphorus removal from wastewater van Loosdrecht,M.C.M.;G.J.Smolders;T.Kuba;J.J.Heijnen https://doi.org/10.1023/A:1000150523030
  40. Appl. Environ. Microbiol v.59 Probing activated sludge with oligonucleotide specific for proteobacteria: Inadequacy of culture-dependent methods for describing microbial community structure Wagner,M.;R.Amann;H.Lemmer;K.H.Schleifer
  41. Appl. Environ. Microbiol v.60 Development fo an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge Wagner,M.;R.Erhart;W.Manz;R.Amann;H.Lemmer;D.Wedi;K.H.Schleifer
  42. Water Sci. Technol v.23 Evaluation of biochemical models for biological excess phosphate removal Wentzel,M.C.;L.H.Lotter;G.A.Ekama;R.E.Loewenthal;G.R.Marias