• Title/Summary/Keyword: Biological properties

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Design Scheme to Develop Integrated Remediation Technology: Case Study of Integration of Soil Flushing and Pneumatic Fracturing for Metal Contaminated Soil (복합복원기술 개발을 위한 설계안 : 중금속 오염토양을 위한 토양세척과 토양파쇄의 통합 사례 연구)

  • Chung, Doug-Young;Yang, Jae-E.
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.1
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    • pp.29-37
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    • 2006
  • In remediation of the contaminated soil, it requires to select at least more than two remediation technologies depending on the fate and transport phenomena through complicated reactions in soil matrix. Therefore, methodologies related to develop the integrated remediation technology were reviewed for agricultural soils contaminated with heavy metals. Pneumatic fracturing is necessary to implement deficiency because soil washing is not effective to remove heavy metals in the subsurface soil. But it needs to evaluate the characteristics such as essential data and factors of designated technology in order to effectively apply them in the site. In the remediation site, the important soil physical and chemical factors to be considered are hydrology, porosity, soil texture and structure, types and concentrations of the contaminants, and fate and its transport properties. However, the integrated technology can be restrictive by advective flux in the area which remediation is highly effective although both soil washing and pneumatic fracturing were applied simultaneously in the site. Therefore, we need to understand flow pathways of the target contaminants in the subsurface soils, that includes kinetic desorption and flux, predictive simulation modeling, and complicated reaction in heterogenous soil.

Characterization and Antimicrobial Activity of Lactic Acid Bacteria Isolated from Vaginas of Women of Childbearing Age (가임기 여성의 질에서 분리한 젖산 세균인 Lactobacillus plantarum UK-3의 특성 및 항균활성)

  • Ahn, Hye-Ran;So, Jae-Seong;Oh, Kye-Heon
    • Korean Journal of Microbiology
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    • v.47 no.4
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    • pp.308-315
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    • 2011
  • The purpose of this work was to examine the antimicrobial activity derived from the lactic acid bacterium, UK-3 isolated from the vaginas of women of childbearing age. Various physiological and biochemical properties of this strain were characterized. Both the BIOLOG system and phylogenetic analysis using 16S rRNA sequencing were utilized for identification, and the strain was designated as Lactobacillus plantarum UK-3, and registered in GenBank as [JK266589]. Growth rate, production of organic acids (e.g., lactic acid and acetic acid), and pH during growth were monitored. The maximum concentrations of lactic acid and acetic acid were approximately 684.11 mM and 174.26 mM, respectively, and pH changed from 7.0 to 3.7 after 72 h of incubation. High performance liquid chromatography was used to confirm lactic acid and acetic acid production. Significant antimicrobial activity of the concentrated supernatant was demonstrated against various Gram-positive (e.g., Staphylococcus aureus, Staphylococcus epidermidis, Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, Neisseria species., Listeria monocytogenes), Gram-negative bacteria (e.g., Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis), and yeast (e.g., Candida albicans) by the plate diffusion method. As a result, the concentrated L. plantarum UK-3 cultures had lower acidity and inhibited the growth of all microorganisms tested, whereas the growth of L. acidophilus was not affected.

The Effect of Different Stocking Rate on Growth, Cast production and Conversion Efficiency of Organic Matter to Tissues of Earthworm (Eisenia fetida L.) (사육밀도의 차이가 지렁이의 생육, 체조직으로의 유기물 전환효율 및 분립생산에 미치는 영향)

  • Lee, Ji-Young;Lee, Ju-Sam
    • Journal of Animal Environmental Science
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    • v.18 no.2
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    • pp.63-74
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    • 2012
  • This experiment was carried out to investigate the effect of different stocking rate on growth, cast production and conversion efficiency of organic matter to tissues of earthworm. The carbon and nitrogen ratio (C/N) of tested Korean cow manure was 25.1, it was estimated an adequate ratio as feed for earthworms. The different stocking rates were 1:8(S-1), 1:16(S-2), 1:32(S-3) 1:64(S-4) 1:128(S-5) and 1:256 (S-6) as the ratios of earthworm fresh weight to biomass of Korean cow manure, respectively. A stocking rate of 1:32(S-3) was obtained a significantly highest values of increasing rate and conversion efficiency of organic matter to earthworm tissues. The mean values of increasin g rate of fresh weight and conversion efficiency of organic matter to earthworm tissues were 10.63 mg/day and 6.65% at the ratio of 1:32(S-3) with a rearing volume was $56.6cm^3$. A stocking rate of 1:8(S-1) was obtained a highest ratio of vermicasts, but showed a negative values of increasing rate and conversion efficiency of organic matter to earthworm tissues, it may due to severely food competition between individuals during the rearing periods. The pH, total nitrogen, available phosphorus, cation exchange capacity and exchangeable cations of vermicasts tended to increase with stocking rate. Especially, available phosphorus, cation exchange capacity and exchangeable cations of vermicasts tended to increase with rearing progressed. Vermicasts have the potential for improving plant growth when amended to container medium and soil according to increased availability of nutrients and improved physicochemical properties.

Evaluation of the Cytotoxic Effects of Resveratrol Treatment with Over-the-counter Drugs on the Hepatic and Intestinal Cells (식이 폴리페놀 성분 resveratrol과 일반의약품의 복합처리에 의한 간 및 장관계 세포독성 평가)

  • Kim, Da-Ram;Kim, Mi-Ri;Hong, Jung-Il
    • Korean Journal of Food Science and Technology
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    • v.42 no.2
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    • pp.217-222
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    • 2010
  • Resveratrol is a natural polyphenolic compound frequently found in grapes. The biological actions of resveratrol have been extensively investigated both in vitro and in vivo. The interactions of resveratrol with commonly-consumed drugs, however, have rarely been studied. In this study, the cytotoxic properties of resveratrol on the hepatic and intestinal cells in the presence of over-the-counter (OTC) drugs, including acetaminophen (AAP), aspirin (Asp), and ibuprofen (Ibu), were evaluated. The cytotoxic effects of resveratrol on hepatic HepG2 and colonic HCT 116 cells were not markdely changed in the presence of AAP, Asp, or Ibu. Conversely, the cytotoxicity of OTC drugs was not affected by resveratrol either. Concentrations of resveratrol below 10 mM significantly increased HepG2 cell growth after 48 or 72 hr incubation; however, the growth-stimulating effect was not observed in the presence of AAP. When HCT 116 cells were treated with OTC drugs before or after resveratrol, the cytotoxic effects were not significantly altered. The present study provides basic information for the potential health effects of the interactions between resveratrol and commonly-consumed OTC drugs.

Effects of Wheat Fiber with Breading on Quality Characteristics of Pork Loin Cutlet (브레딩시 밀 식이섬유 첨가가 돈육 등심 돈가스 품질특성에 미치는 영향)

  • Park, Kwoan-Sik;Choi, Yun-Sang;Kim, Hyun-Wook;Song, Dong-Heon;Lee, Soo-Yeon;Choi, Ji-Hun;Kim, Cheon-Jei
    • Food Science of Animal Resources
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    • v.32 no.4
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    • pp.504-511
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    • 2012
  • The effects of the addition of wheat fiber on physico-chemical properties and sensory characteristics of pork loin cutlets were investigated to develop pork loin cutlets formulated with different concentrations of wheat fiber (0, 1, 2, 3, and 4%). The moisture content, ash content, pH, and lightness of pork loin cutlets increased with increasing wheat fiber levels (p<0.05). However, the fat content, calorie, redness, hardness, cohesiveness, gumminess, and chewiness decreased with increasing wheat fiber levels (p<0.05). The protein content, yellowness, and springiness of pork loin cutlets did not show significant difference (p>0.05). The sensory evaluation shows the greatest overall acceptability was ranked at the pork loin cutlets added with wheat fiber at 3%. Pork loin cutlets with added wheat fiber could be commercially acceptable meat products, and can be improved quality characteristics. The best results were obtained for pork loin cutlets with addition of 3% wheat fiber.

Effects of Dietary Fiber Extracts from Brewer's Spent Grain on Quality Characteristics of Chicken Patties Cooked in Convective Oven

  • Kim, Hyun-Wook;Hwang, Ko-Eun;Song, Dong-Heon;Lee, Soo-Yeon;Choi, Min-Sung;Lim, Yun-Bin;Choi, Ji-Hun;Choi, Yun-Sang;Kim, Hack-Youn;Kim, Cheon-Jei
    • Food Science of Animal Resources
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    • v.33 no.1
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    • pp.45-52
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    • 2013
  • Brewer's spent grain (BSG) is a by-product of beer manufacturing. This study was conducted to evaluate the effect of dietary fiber extracts from brewer's spent grain on quality characteristics of chicken patties. The total fiber content of BSG dietary fiber extracts after extraction increased from 58.11% to 68.57%, and the extracted dietary fiber extracts were added to chicken patties at 0, 1, 2, 3, and 4%, respectively. The effects of the BSG dietary fiber extracts on pH, color, cooking loss, reduction in patty diameter, salt-soluble protein solubility, texture, and sensory characteristics of chicken patties were evaluated. The addition of BSG dietary fiber extracts decreased pH and lightness values, and increased redness and yellowness. Chicken patties formulated with 3-4% BSG dietary fiber extracts had the lowest cooking loss among all treatments (p<0.05). The diameter of chicken patties was not affected by the addition of BSG dietary fiber extracts. Additionally, no significant difference was observed in salt-soluble protein solubility after adding 3% BSG dietary fiber extracts compared to that in the control. Textural and sensory properties were different among the chicken patties, and the 3% BSG dietary fiber-added chicken patty had the highest acceptability. Our results indicate that 3% BSG dietary fiber extract can be used as a good source of dietary fiber for improving the quality characteristics of chicken patties.

A Method for Protein Functional Flow Configuration and Validation (단백질 기능 흐름 모델 구성 및 평가 기법)

  • Jang, Woo-Hyuk;Jung, Suk-Hoon;Han, Dong-Soo
    • Journal of KIISE:Computing Practices and Letters
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    • v.15 no.4
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    • pp.284-288
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    • 2009
  • With explosively growing PPI databases, the computational approach for a prediction and configuration of PPI network has been a big stream in the bioinformatics area. Recent researches gradually consider physicochemical properties of proteins and support high resolution results with integration of experimental results. With regard to current research trend, it is very close future to complete a PPI network configuration of each organism. However, direct applying the PPI network to real field is complicated problem because PPI network is only a set of co-expressive proteins or gene products, and its network link means simple physical binding rather than in-depth knowledge of biological process. In this paper, we suggest a protein functional flow model which is a directed network based on a protein functions' relation of signaling transduction pathway. The vertex of the suggested model is a molecular function annotated by gene ontology, and the relations among the vertex are considered as edges. Thus, it is easy to trace a specific function's transition, and it can be a constraint to extract a meaningful sub-path from whole PPI network. To evaluate the model, 11 functional flow models of Homo sapiens were built from KEGG, and Cronbach's alpha values were measured (alpha=0.67). Among 1023 functional flows, 765 functional flows showed 0.6 or higher alpha values.

Optimization on the Preparation Conditions of Instant Rice Gruel Using Paecilomyces japonica mycelia (동충하초(Paecilomyces japonica)균사체를 이용한 즉석죽 제조조건의 최적화)

  • 이기동;김숙경;정용진;윤광섭;신승렬
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.870-876
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    • 2001
  • Cordyceps is very famous of its board biological activities and vital function in traditional medicines. Fourdimensional response surface methodology was used for monitering of instant rice gruels. The organolepitc color of instant rice gruel prepared using mushroom and glutinuous rice showed the maximum score in 38.52% rate of glutinous rice 321.46 mL content of added water and 1.30 g content of P. japonica mycelia. The organoleptic taste of instant rice gruel prepared using mushroom and glutinous rice showed the maximum score in 64.86% rate of glutinous rice 270.66 mL content of added water and 2.32 g content of P. japonica mycelia. The organoleptic flavor of instant rice gruel prepared using mushroom and glutinous rice was maximized in 36.75% rate of glutinous rice, 323.40 mL content of added water and 1.42 g content of P. japonica mycelia. The organoleptic mouth-feel of instant rice gruel prepared using mushroom and glutinous rice was maximized in 65.33% rate of glutinous rice 275.37 mL content of added water and 2.41 g content of P. japonica mycelia. The organoleptic overall palatability of instant rice gruel prepared using mushroom and glutinous rice showed the maximum score in 40.94% rate of glutinous rice 352.79 mL content of added water and 1.27 g content of P. japonica mycelia.

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Biological Activity in Functional Cosmetic of Purple Sweet Potato Extracts (자색 고구마추출물의 기능성 화장품 활성)

  • Choi, Jae-Hong;Kim, Jin-Sung;Jo, Bun-Sung;Kim, Jeung-Hoan;Park, Hye-Jin;An, Bong-Jeun;Kim, Myung-Uk;Cho, Young-Je
    • Food Science and Preservation
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    • v.18 no.3
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    • pp.414-422
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    • 2011
  • The optimum condition for phenolics contents extraction from purple sweet potato was 12 hrs. in 50% ethanol. The electron donating scavenging activities (DPPH), ABTS radical cation decolorization (ABTS) and antioxidant protection factor (PF) of Jami, Yeonjami and Shinjami were higher than general sweet potato, and thiobarbituric acid reaction substance (TBARs) was below 30%. The minimum inhibitory concentration (MIC) against Staphylococcus aureus and Escherichia coli on skin were each 5,000 and 2,500 ppm in all purple sweet potatoes, and MIC of Jami was the lowest as 2,500 ppm against Staphlococcus epidermidis. The whitening (tyrosinase inhibitory) activity of purple sweet potatoes was the highest as 62.5% and 48.7% in Jami water and ethanol extracts. The anti-inflammation (hyaluronidase inhibitory) activity of purple sweet potatoes was the highest as 25.3% and 94.4% in Jami water and ethanol extracts. The safety of cosmetic with Jam; extracts was assessed by various safety profiles. pH and viscosity change of essence for 90 days was not detected. Cosmetic was stable to temperature and light for 90 days. The result to measure changes of skin color and pore size of the skin was that an aged skin was more effective than young skin.

Monitoring on Alcohol Fermentation Properties of Apple Juice for Apple Vinegar (사과식초 제조를 위한 사과주스의 알코올발효 특성 모니터링)

  • Shin, Eun-Jeong;Kang, Bok-Hee;Lee, Sang-Han;Lee, Dong-Sun;Hur, Sang-Sun;Shin, Kee-Sun;Ki, Seong-Ho;Son, Seok-Min;Lee, Jin-Man
    • Food Science and Preservation
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    • v.18 no.6
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    • pp.986-992
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    • 2011
  • The alcohol fermentation of apple juice was optimized as a preliminary study for the production of natural apple cider vinegar. To gain an optimal fermentation yield, a central composite design was used to investigate the effects of the independent variables [initial Brix (12/14/16/18/20, $X_1$), fermentation time(48/54/60/66/72h, $X_2$), and fermentation temperature(24/26/28/30/$32^{\circ}C$, $X_3$)] on the dependent variables (alcohol content, reducing sugar, Brix, acidity). The alcohol content was 3.4-6.4%, the reducing sugar was 1.93-6.24%, and the Brix was $6.1-13.8^{\circ}$. The alcohol content was mainly affected by the fermentation temperature and increased along with the fermentation time and temperature. The amount of the reducing sugar was significantly affected by the initial Brix and fermentation temperature. The optimal conditions for the alcohol content were found to be 15.22 initial Brix, 64.97 h fermentation time, and $31.56^{\circ}C$ temperature.