• 제목/요약/키워드: Biological analysis

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들깻잎에서 Triazole계 살균제의 생물학적 반감기와 물리화학적 특성과의 상관관계 (Correlation between physicochemical properties and biological half-life of triazole fungicides in perilla leaf)

  • 이상협;곽세연;황정인;김효정;김태화;김장억
    • Journal of Applied Biological Chemistry
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    • 제62권4호
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    • pp.407-415
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    • 2019
  • 들깻잎에 살포된 triazole계 살균제 6종에 대하여 생물학적 반감기를 산출하고 분해 및 소실 속도를 확인하였으며, 통계분석으로 생물학적 반감기와 농약의 물리화학적 특성간의 상관관계를 확인하였다. 확립된 분석법으로 6종 농약의 회수율 시험 결과 84.8-104.9%로 잔류농약분석법 기준을 만족하였다. 들깻잎에 살포한 농약 6종의 생물학적 반감기는 first-order kinetics model으로 산출하였으며, 그 결과 6.4-15.1일로 나타났다. 산출된 생물학적 반감기와 6종의 농약의 물리화학적 특성을 주성분 분석으로 상관성을 확인하였을 때, pKa, Log P 및 생물학적 반감기가 PC1에 영향을 받아 상관성이 있는 것으로 나타났다. Spearman rank-order correlation으로 생물학적 반감기와 물리화학적 특성간의 상관계수를 산출하였을 때, 생물학적 반감기와 pKa는 R2 = -0.928, p <0.01로 나타나, 생물학적 반감기는 pKa와 상관성이 있는 것으로 나타났다.

A Study on the Quantitative Regularity Measures That Are Suitable for Biological Signal Analysis - Standard Data and 24 Hour R-R interval Analysis

  • 남영한;이종민;한주만;박광석
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1998년도 추계학술대회
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    • pp.197-198
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    • 1998
  • We tested the capability of Pointwise Correlation Dimension(PD2), Approximate Entropy (ApEn) and LZ complexity, as alternative measures of a biological signal. For this purpose, we analyzed standard data and a healthy child's 24-hour heart rate variability. Our conclusion is as follows. First, PD2, ApEn and LZ complexity can be used for discerning chaotic attractor, white noise, and periodic signal. Second, these measures show different characteristics on day and night. Third, these measures can be used for detecting time-varying characteristics of biological signals.

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Biological Control Activity of Two Isolates of Pseudomonas fluorescens against Rice Sheath Blight

  • Choi Gyung-Ja;Kim Jin-Cheol;Park Eun-Jin;Choi Yong-Ho;Jang Kyoung-Soo;Lim He-Kyoung;Cho Kwang-Yun;Lee Seon-Woo
    • The Plant Pathology Journal
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    • 제22권3호
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    • pp.289-294
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    • 2006
  • Two isolates of mucous bacteria, mc75 and pc78, were isolated from fungal culture plate as culture contaminants with an interesting swarming motility. Both isolates were identified as Pseudomonas fluorescens based on microscopy, biochemical analysis, Biolog test and DNA sequence analysis of the 16S rRNA gene. Both strains have the exactly the same 16S rRNA gene sequences, and yet their biological control activity were not identical each other. In vitro analysis of antagonistic activity of two isolates against several plant pathogenic fungi indicated that both produced diffusible and volatile antifungal compounds of unknown identities. Treatment of the bacterial culture of P. fluorescens pc78 and its culture filtrate exhibited a strong biological control activity against rice sheath blight in vivo among six plant diseases tested. More effective disease control activity was obtained from treatment of bacterial culture than that of culture filtrate. Therefore, in addition to antifungal compound and siderophore production, other traits such as biofilm formation and swarming motility on plant surface may contribute to the biological control activity of P.fluorescens pc78 and mc75.

A novice’s guide to analyzing NGS-derived organelle and metagenome data

  • Song, Hae Jung;Lee, JunMo;Graf, Louis;Rho, Mina;Qiu, Huan;Bhattacharya, Debashish;Yoon, Hwan Su
    • ALGAE
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    • 제31권2호
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    • pp.137-154
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    • 2016
  • Next generation sequencing (NGS) technologies have revolutionized many areas of biological research due to the sharp reduction in costs that has led to the generation of massive amounts of sequence information. Analysis of large genome data sets is however still a challenging task because it often requires significant computer resources and knowledge of bioinformatics. Here, we provide a guide for an uninitiated who wish to analyze high-throughput NGS data. We focus specifically on the analysis of organelle genome and metagenome data and describe the current bioinformatic pipelines suited for this purpose.

Web Services Based Biological Data Analysis Tool

  • Kim, Min Kyung;Choi, Yo Hahn;Yoo, Seong Joon;Park, Hyun Seok
    • Genomics & Informatics
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    • 제2권3호
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    • pp.142-146
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    • 2004
  • Biological data and analysis tools are accumulated in distributed databases and web servers. For this reason, biologists who want to find information from the web should be aware of the various kinds of resources where it is located and how it is retrieved. Integrating the data from heterogeneous biological resources will enable biologists to discover new knowledge across the specific domain boundaries from sequences to expression, structure, and pathway. And inevitably biological databases contain noisy data. Therefore, consensus among databases will confirm the reliability of its contents. We have developed WeSAT that integrates distributed and heterogeneous biological databases and analysis tools, providing through Web Services protocols. In WeSAT, biologists are retrieved specific entries in SWISS-PROT/EMBL, PDB, and KEGG, which have annotated information about sequence, structure, and pathway. And further analysis is carried by integrated services for example homology search and multiple alignments. WeSAT makes it possible to retrieve real time updated data and analysis from the scattered databases in a single platform through Web Services.

Analysis of Ingredients and biological activities confirm Process for Personalized Diet Offering Service: Basic ingredients Analysis and biological Activities of Grifola frondosa

  • Hong, Seok Chan;Hwang, You Jin;Kang, Un Gu
    • 한국컴퓨터정보학회논문지
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    • 제24권8호
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    • pp.113-121
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    • 2019
  • The personalized meal service is being developed to prevent and alleviate illnesses according to the individual's health condition. However, the current meal does not provide a fully customized service to individuals and a diet that meets the consumer's information needs. The cause is the lack of information on the ingredients and the difficulty of comparative analysis between the materials. Therefore, in this study, we propose basic analysis process for basic information acquisition and database construction for food composition before providing personalized food. In this study, we investigated the content of carbohydrate, reducing sugar and protein as basic components of Grifola frondosa and investigate the content of polyphenol as a biological active ingredients. Respectively. Studies on the hypoglycemic effect of the diabetic rat model have been carried out in relation to the prevention of diseases. Based on the results of this study, it is also possible to obtain information on the basic ingredients of the food and to analyze the information on the content and activity of the biological active ingredients. Using animal models, information on disease prevention and mitigation was also available. The process introduced in this study is applied to various food materials, accumulating data, and utilizing Database, this results will be an excellent tool for providing more efficient service by providing a proper dietary composition for consumers.

Benford's Law and its Potential for Data Verification in Ecological Monitoring

  • Tae-Jun Choi;Woong-Bae Park;Dae-Hee Kim;Dohee Lee;Yuno Do
    • Proceedings of the National Institute of Ecology of the Republic of Korea
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    • 제5권2호
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    • pp.43-49
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    • 2024
  • Ecological monitoring provides indispensable data for biodiversity conservation and sustainable resource management. However, the complexity and variability inherent in ecological monitoring data necessitate robust verification processes to ensure data integrity. This study employed Benford's Law, a statistical principle traditionally used in fields such as finance and health sciences, to evaluate the authenticity of ecological monitoring data related to the abundance of migratory bird species across various locations in South Korea. Benford's Law anticipates a specific logarithmic distribution of leading digits in naturally occurring numerical datasets. Our investigation involved two stages of analysis: a first-order analysis considering the leading digit and a second-order analysis examining the first two digits of bird population counts. While the first-order analysis displayed moderate conformity to Benford's Law that suggested overall data integrity, the second-order analysis revealed more pronounced deviations, indicating potential inconsistencies or inaccuracies in certain subsets of the data. Although our data did not perfectly align with Benford's Law, these deviations underscore the complex nature of ecological research, which is influenced by a multitude of environmental, methodological, and human factors.

Overexpression of Fish DRG2 Induces Cell Rounding

  • Park, Jeong-Jae;Cha, Seung-Ju;Ko, Myung-Seok;Cho, Wha-Ja;Yoon, Won-Joon;Moon, Chang-Hoon;Do, Jeong-Wan;Kim, Sung-Bum;Hebok Song
    • Journal of Microbiology
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    • 제40권4호
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    • pp.295-300
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    • 2002
  • Previously, we reported induced expression of developmentally regulated CTP-binding protein 2 (DRG2) in fish cells at the late stage of rhabdovirus infection. To investigate the biological role of fish DRG2 (fDRG2), we transfected CHSE-214 cells with an expression vector containing complete fDRG2 fused to the N-terminal end of an enhanced green fluorescent protein (EGFP). Low level expression of fDRG2-EGFP did not induce morphological change or cell death. However, a high level expression of fDRG2-EGFP induced cell rounding and caused depletion of the cell population in FACS analysis. Several truncated fragments were fused to EGFP. FACS analysis was conducted to determine the presence of cells expressing high levels of the resulting chimera. While cells expressing a high level of N-terminus were detected, those expressing high levels of the C-terminal fragment 243-290 containing the G4 motif were absent in FACS analysis. Based on these observations, we propose that overexpression of fDRG2 may induce cell rounding, a representative cytopathic effect of virus-infected cells in the late stage of infection and the C-terminus of the fDRG2 is essential for this function.