• 제목/요약/키워드: Biological analysis

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Three New Recorded Species of the Physalacriaceae on Ulleung Island, Korea

  • Park, Ki Hyeong;Kim, Changmu;Kim, Minkyeong;Kim, Nam Kyu;Park, Jae Young;Eimes, John A.;Cho, Hae Jin;Han, Sang-Kuk;Lim, Young Woon
    • Mycobiology
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    • 제45권1호
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    • pp.9-14
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    • 2017
  • Most known species in the Physalacriaceae are saprotrophs that grow on decaying leaves and wood, and approximately 21 genera in the Physalacriaceae have been reported worldwide. During an ongoing survey of indigenous fungi in Korea, four specimens belonging to the Physalacriaceae were collected on Ulleung Island. These specimens were identified as three species based on morphological characteristics and molecular analysis of rDNA-internal transcribed spacer sequences. Three species in three genera were shown to be new records in Korea: Hymenopellis orientalis, Paraxerula hongoi, and Ponticulomyces orientalis. The latter two are the first records of these genera in Korea. In this study, we provide detailed morphological descriptions of these species and describe their phylogenetic position within the Physalacriaceae.

New Records of Aspergillus allahabadii and Penicillium sizovae from Crop Field Soil in Korea

  • Tagele, Setu Bazie;Adhikari, Mahesh;Gurung, Sun Kumar;Lee, Hyun Gu;Kim, Sang Woo;Kim, Hyun Seung;Ju, Han Jun;Gwon, Byeong Heon;Kosol, San;Lee, Hyang Burm;Lee, Youn Su
    • Mycobiology
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    • 제46권4호
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    • pp.297-304
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    • 2018
  • Two new records of Trichocomaceae, namely Aspergillus allahabadii and Penicillium sizovae, were isolated in 2016 during a survey of fungal diversity in different crop fields locations in Gyeongnam, Korea. These species were identified based on morphological characters and phylogenetic analysis using internal transcribed spacer region and ${\beta}-tubulin$-encoding gene sequence data. A. allahabadii and P. sizovae have not yet been reported in Korea. Thus, this is the first report of these species in Korea, and their descriptions as well as details of their morphological characters are presented.

남부지역 소형 관개용 못들에서의(둠벙) 동물플랑크톤 군집특성 조사 및 평가 (Observation and Evaluation of Zooplankton Community Characteristics in the Petite Ponds (Dumbeong) for Irrigation: A Case Study in Goseong Region of South Korea)

  • 김항아;최종윤;김성기;도윤호;주기재;김동균;김현우
    • 생태와환경
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    • 제45권4호
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    • pp.490-498
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    • 2012
  • This study demonstrates the investigation of zooplankton communities (e.g. rotifers, cladocerans and copepods) and environmentally related driving factors (e.g. elevation, area size, water depth, types of dike construction, and bottom substrates). We hypothesized that zooplankton community structure and composition would be influenced by ambient driving forces in different scales of the irrigation ponds (Dumbeong). A total of 66 zooplankton species/groups (56 rotifers, 9 cladocerans, 1 copepods) were found and identified at 45 Dumbeong of Goseong region (i.e. Goseong-gun) in 2011. The rotifers occupied 84.9% of the total zooplankton abundance. We could categorize a clear separation of zooplankton communities into 4 different patterns based on cluster analysis. Zooplankton diversities in Dumbeongs were lower than those in natural ponds or wetlands. In addition, community structure of zooplankton was also simpler and had a broken stick distribution based on SHE analysis. Species composition in each Dumbeong was not significantly discriminated each other. The result of canonical correspondence analysis (CCA) pinpointed that significant influential variables upon zooplankton community were dissolved oxygen percent saturation, pH, and Dumbeong's material. This study indicated that morphological type of the Dumbeong and its water quality could determine the community structure of zooplankton. Furthermore, the connectivity between ambient habitats and materials could be necessary to be rigorously considered in respect to producing the Dumbeongs to subsidize alternative habitats for wetland ecosystem in freshwater landscape.

특허정보에 의한 제올라이트 분리막 연구동향 고찰 (Analysis of Zeolite Membrane Using Patent Information)

  • 임은정;김성현;김상곤;현동훈;박선희
    • 청정기술
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    • 제18권3호
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    • pp.307-311
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    • 2012
  • 특허권은 강력한 자산이다. 삼성과 애플의 특허 소송이 대표적인 사례이다. 따라서 각국에서 지식재산권이 강화되고 특허가 강조되고 있다. 특허분석의 기본은 특허정보를 활용하는 것이다. 특허정보는 새로운 기술을 제공하고 국제코드인 국제특허분류에 의해 구분되어 있다는 것이다. 논문에서는 국제특허분류코드를 이용하여 제올라이트 기술 분석에 대한 국내외 특허를 분석하였다. 1992년부터 2011년까지의 한국, 미국, 일본, 중국 및 유럽에서 출원된 특허에 대하여 출원국별, 출원인별, 연도별, 세부기술 분야로 분류하여 비교함으로써 기술개발 현황을 분석하였다.

Functional Characterization of Drosophila melanogaster CYP6A8 Fatty Acid Hydroxylase

  • Sang-A Lee;Vitchan Kim;Byoungyun Choi;Hyein Lee;Young-Jin Chun;Kyoung Sang Cho;Donghak Kim
    • Biomolecules & Therapeutics
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    • 제31권1호
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    • pp.82-88
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    • 2023
  • Genomic analysis indicated that the genome of Drosophila melanogaster contains more than 80 cytochrome P450 genes. To date, the enzymatic activity of these P450s has not been extensively studied. Here, the biochemical properties of CYP6A8 were characterized. CYP6A8 was cloned into the pCW vector, and its recombinant enzyme was expressed in Escherichia coli and purified using Ni2+-nitrilotriacetate affinity chromatography. Its expression level was approximately 130 nmol per liter of culture. Purified CYP6A8 exhibited a low-spin state in the absolute spectra of the ferric forms. Binding titration analysis indicated that lauric acid and capric acid produced type I spectral changes, with Kd values 28 ± 4 and 144 ± 20 µM, respectively. Ultra-performance liquid chromatography-mass spectrometry analysis showed that the oxidation reaction of lauric acid produced (ω-1)-hydroxylated lauric acid as a major product and ω-hydroxy-lauric acid as a minor product. Steady-state kinetic analysis of lauric acid hydroxylation yielded a kcat value of 0.038 ± 0.002 min-1 and a Km value of 10 ± 2 µM. In addition, capric acid hydroxylation of CYP6A8 yielded kinetic parameters with a kcat value of 0.135 ± 0.007 min-1 and a Km value of 21 ± 4 µM. Because of the importance of various lipids as carbon sources, the metabolic analysis of fatty acids using CYP6A8 in this study can provide an understanding of the biochemical roles of P450 enzymes in many insects, including Drosophila melanogaster.

국내 주요 간호학회지에 게재된 생물학적 측정법(Biological measurement)을 이용한 연구에 대한 분석 (An Analysis on the Researches Using Biological Measurement in Major Korean Nursing Journals)

  • 김주현;최명애;김윤경;김진학;김희승;박미정;신기수;안경주;이윤미;이경숙;정재심;채영란;홍해숙
    • Journal of Korean Biological Nursing Science
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    • 제8권2호
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    • pp.61-72
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    • 2006
  • Purpose: This study was to examine the frequency, distribution and characteristics of researches using biological measurement published from 2000 to 2004 in 10 major nursing journals in Korea, Design: Literature analysis. Method: Journals including papers using biological measurements, publishing year, research design and outcome variables were analyzed. Results: 1. Researches using biological measurement were 318(13.3%). 2. Researches using biological measurement in the Korean Academy of Nursing were highest(97papers, 17.5%) among the nursing journals. The proportion of papers using biological measurement to total number of papers was the highest in the Journal of Korean Biological Nursing Science as 77.3%(51papers). 3. The 233 papers(73.3%) were experimental researches among 318 papers using biological measurement which showed the highest proportion. 4. Patients were highest subjects of researches using biological measurement(197papers, 61.9%). 5. Blood test was most frequently used as physiological variables from 2001 to 2004. Conclusion: Researches using biological measurement of 10 Korean Nursing Journals in year 2000-year 2004 were very low. We need more researches using biological measurement to provide more objective evidence for nursing practice.

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인체 착용형 무선 단말기에 대한 노출량 해석 (Analysis of SAR for body-mounted mobile phones)

  • 박민영;고채옥;백정기
    • 한국전자파학회:학술대회논문집
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    • 한국전자파학회 2005년도 종합학술발표회 논문집 Vol.15 No.1
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    • pp.425-428
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    • 2005
  • A variety of wireless devices are commercially available now. Most of studies, however, have been directed to the biological effects of mobile-phone EMF. In this study, dosimetric analysis for wireless devices of head-mounted display type and a wristwatch type were made to investigate possible biological effects of these devices. SAR (Specific Absorption Rate) distributions were calculated using FDTD (Finite Difference Time Domain) method, for adult human models such as standard Korean human model and VHP(Visible Human Project) model, as well as scaled models. Measurements were also performed for SAM phantom wearing a simplified prototype for a wireless device for validation of the simulation results. It has been found that children are more vulnerable to such exposure, and these devices could cause some biological effects for relatively lower power compared to conventional mobile pones.

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Chemical Analysis and Biological Activity of Endotoxin from Vibrio vulnificus

  • Lee, Bong-Hun
    • Journal of Life Science
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    • 제11권1호
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    • pp.27-29
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    • 2001
  • Vibrio vulnificus endotoxin was extracted, analyzed the chemical composition, tested its biological activity, and compared to those of Escherichia coli and Salmonella typhimurium. The major fatty acid of three endotoxins were different each other; V. vulnificus endotoxins were different each other; V. vulnificus endotoxin was myristric acid (C14:0), E. coli was lauric acid (C12:0), S. typhimurium was capric acid (C10:0). The biological activities of V. vulnificus endotoxin were similar to those of E. coli and S. typhimurium in terms of the gelation activity of the Limulus amebocyte lysate and the lethal toxicity. But the result of enzyme (AST, ALT, and LDH) analysis showed that the enzyme activity of V. vulnificus endotoxin was similar to that of E. coli, but smaller than that of S. typimurium.

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Mercury Adsorption of Chemically Modified Polysaccharide from Methylobacterium organophilum

  • Lee, Jung-Gul;Kim, Sang-Yong;Oh, Deok-Kun;Kim, Jung-Hoe
    • Applied Biological Chemistry
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    • 제41권4호
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    • pp.209-212
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    • 1998
  • Methylan, a polysaccharide produced from Methylobacterium organophilum, was chemically modified by adding diethylaminoethyl (DEAE) group to the backbone of methylan. The structure of DEAE-methylan was determined by measuring its nitrogen content obtained from an elemental analysis. From the analysis of mass spectrum, the DEAE group in DEAE-methylan was also confirmed by determining diethylaminoethene as a separate form of DEAE. Mercury adsorption of DEAE-methylan was higher than that of native methylan. This fact was valid for a variety of pH, reaction times, metal concentrations, and polysaccharide concentrations. In particular, native methylan and DEAE-methylan adsorbed 16% (w/w) and 18% (w/w) for mercury after 30 min at pH 7, respectively. The increase in mercury adsorption of DEAE-methylan may be resulted from mercury adsorption by the lone pair electron of nitrogen atom in DEAE group.

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