• 한국수산과학회지
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• 제56권1호
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• pp.133-139
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• 2023

The advantage of biofloc technology (BFT) in aquaculture is in the prevention of pathogenic transmission. In this study, we performed an investigation on viral, bacterial, and microsporidian parasite infections targeting a total of 194 whiteleg shrimp Litopenaeus vannamei reared in seven BFT-farms on the west coast of Korea in 2021. Hepatopancreatic and cuticular epithelium and pereiopods tissues of shrimp were tested for the four pathogens, Enterocytozoon hepatopenaei (EHP), Vibrio parahaemolyticus causing Acute Hepatopancreatic necrosis disease (VP_AHPND), white spot syndrome virus (WSSV), and hepatopancreatic parvovirus (HPV). The microsporidian parasite EHP was detected in the hepatopancreatic tissue of BFT whiteleg shrimp in the Ganghwa region, whereas no other pathogenic bacteria or virus was detected on the shrimp in the seven BFT-farms. As a result of bacterial flora in the rearing water of BFT whiteleg shrimp using DNA microbiome technology, V. chemaguriensis and V. alfacsensis were contained at 0.05% and 0.01%, respectively, but no VP_AHPND was detected. These findings will serve as a basis for supporting safe BFT-aquaculture of whiteleg shrimp.

• 한국수산과학회지
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• 제54권4호
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• pp.418-425
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• 2021

The effects of an aquaponic system based on biofloc technology (BFT-AP) were analyzed for eel Anguilla japonica as aquaculture species and caipira Lactuca sativa as the cultivated crops. The rate of weight gain rate by the eels was 178% (BFT-AP 200 head) > 136% (BFT-AP 100 head) > 100% (BFT). The eel body weight in the BFT-AP (200 head) significantly increased when compared to the BFT only eel group (P<0.05). The weight of the upper layer of caipira was 91±8.5 g (200 head) > 90±8.9 g (100 head) > 48±8.3 g (Hydroponic crop, HP). The crop growth in all BFT-AP groups was higher than the control, the hydroponic group. The total ammonia nitrogen (TAN) and NO₂^--N concentrations decreased in the BFT-AP group when compared to the BFT group. It was possible to remove nitric acid from the aquaponics system and reuse it as eel rearing water. Although some nutrient concentrations were low in BFT when compared to HP, the nutrient concentration was sufficient for plant growth. The results show that BFT has the potentially to provide a sustainable aquaponic system.

• 한국수산과학회지
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• 제52권2호
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• pp.159-172
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• 2019

This study investigated the effects of dietary monobasic potassium phosphate (MKP) on the growth of the far eastern catfish Silurus asotus and four leafy vegetables in a hybrid biofloc technology aquaponic system. To an experimental diet containing 45% protein and 7% lipid, 1, 2, 3 or 4% MKP was added and was designated as MKP1, MKP2, MKP3, and MKP4, respectively. The optimum MKP levels were determined for the growth of fish and four leafy vegetables over 10 weeks. After the 10-week feeding trial, weight gain, feed efficiency, specific growth rate and protein efficiency ratio were higher in the fish groups fed MKP2 and MKP3 than in the other groups (P<0.05). The growth of the four leafy vegetables was also higher in the fish groups fed MKP2 and MKP3. Water quality [dissolved oxygen, pH, water temperature, electrical conductivity, turbidity, total ammonia nitrogen (TAN), $NO_2-N$, $NO_3-N$ and $PO_4-P$] was measured six times a week using a portable water quality meter and reagent measurements. The TAN (4.58-20.40 mg/L), $NO_3-N$ (24.12-52.40 mg/L) and $PO_4-P$ (20.38-48.48 mg/L) levels increased with time, while the $NO_2-N$ level remained below 0.1 mg/L throughout the study.

• 한국수산과학회지
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• 제55권5호
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• pp.541-548
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• 2022

Biofloc technology (BFT) systems provides an additional protein source for fish by converting waste in microbial flocs. Therefore, we aimed to determine the difference in the protein levels of the diet of Far Eastern catfish Silurus asotus reared in BFT and FW systems. We applied a two-way (six protein levels × two aquaculture systems) ANOVA design. We formulated six experimental diets to contain different protein levels (CPs)- 20%, 25%, 30%, 35%, 40% and 45%-referred to as the CP20, CP25, CP30, CP35, CP40 and CP45 diets, respectively. We randomly distributed 900 fish into 36 (18 each for BFT and FW), 300 L tanks (25 fish/tank) with three replications of each diet for 4 weeks. The weight gain (WG) and specific growth rate (SGR) considerably improved as the dietary protein level increased in the formulated diets. The BFT fish showed higher growth performance with any experimental diet than the FW fish. The growth performance of the fish fed the BFT-CP35 diet was higher than that of the fish fed the FW-CP45 diet. In conclusion, the cultivation of Far Eastern catfish (S. asotus) in BFTs can be carried out under reduced dietary protein levels.

• Journal of Microbiology and Biotechnology
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• 제26권8호
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• pp.1420-1427
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• 2016

Geosmin and 2-methylisoborneol (2-MIB) are two of the most common taint compounds that adversely affect the quality of aquacultural animals. In the present study, 94% of geosmin and 97% of 2-MIB in suspended growth reactors producing bioflocs (SGRs) with aquaculture waste were removed after inoculation with Bacillus subtilis, significantly higher than that of control SGRs (70% of geosmin and 86.4% of 2-MIB). The lowest concentrations of geosmin and 2-MIB achieved in the effluent of the SGRs were 2.43 ± 0.42 ng/l and 2.23 ± 0.15 ng/l, respectively. The crude protein content of the bioflocs produced in the SGRs was 35 ± 4%. The NH₄⁺-N and NO₂^--N concentrations in the effluent of the reactors were 1.13 ± 0.21 mg/l and 0.42 ± 0.04 mg/l, respectively. These results suggest that inoculated with Bacillus subtilis, SGRs have a better performance to reuse the nitrogen in fish waste and to remove geosmin and 2-MIB from the culture water efficiently.

• 생명과학회지
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• 제28권12호
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• pp.1424-1431
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• 2018

본 연구에서는 BFT (biofloc technology) system 수조로부터 분리된 미생물(Bacillus sp.)을 이용하여 Marine snow와 유사한 유기물 분해산물을 만들어 뱀장어 자어먹이로 활용 가능성을 조사하였다. 유기원으로는 계란, 참다랑어, 뱀장어, 담치, 갯지렁이, 멍게, 바지락 그리고 새우를 이용하여 8 종류의 유기물 분해산물(HE, TM, EM, PC, TA, MS, HA, SP)를 생산했다. 생산된 유기물 분해산물은 특정한 형태는 나타나지 않았으며, 크기도 $10-20{\mu}m$의 미세한 것부터 $100{\mu}m$ 이상 되는 것까지 다양하였다. 부화 후 20일부터 다양한 유기물 분해산물을 이용하여 제조한 4 종류의 먹이(A, B, C, D-type)를 급이한 결과 A, B, C-type의 먹이를 급이한 뱀장어 유생은 각각 부화 후 37일, 39일, 37일까지 생존하였으나, D-type의 먹이를 급히한 뱀장어 유생은 부화 후 60일 동안 생존하였다. 각 먹이의 단백질 함량은 비슷하였지만, D-type 먹이의 n-3 HUFA 농도는 다른 type의 n-3 HUFA 농도보다 약 2배 높았다.

• 청정기술
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• 제7권1호
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• pp.35-42
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• 2001

본 연구에서는 zeolite를 활성슬러지 공정에 주입할 경우 zeolite가 biofloc에 미치는 영향에 대해 알아보기 위해 수행되었다. 두 반응기를 동일 조건으로 운전하였으며 하나는 표준 활성슬러지 공정 (CU)으로, 다른 하나는 zeolite를 폭기조내 4,000 mg/l로 유지 (ZU)하며 비교하였다. 슬러지 플럭 입경을 측정한 결과, 두 반응기 모두 SRT (Solid Retention Time)가 증가함에 따라 플럭 크기도 비례하여 증가하였으나 ZU가 모든 조건에서 CU보다 작은 평균 입경을 나타내었다. 한편, 두 반응기에 대해 슬러지 침전성을 비교한 결과, ZU는 SRT변화에 관계없이 3.4~11m/h의 침전속도를 나타내며 SVI(Sludge Volume Index)도 50~108 ml/g으로 양호한 침전상태를 보였다. 그러나 CU는 SRT 증가에 따라 슬러지 침전성이 큰 영향을 받았고 SRT 40일에서 Sphaerotilus가 우점종을 나타내었으며 이 결과, 슬러지가 전혀 침전하지 않았다.

• 한국해양생명과학회지
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• 제7권2호
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• pp.196-204
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• 2022

본 연구는 Bacillus subtilis를 활용한 바이오플락 양식 기술(Biofloc technology, BFT)을 이용하여 대농갱이(Leiocassis ussuriensis) 양식의 가능성을 확인하기 위해 90일 동안 생존, 성장지수와 사육수 수질의 변화를 관찰하였다. 대농갱이를 입식하기 전 BFT 수 제조를 위해 실험수조에 사료와 당밀을 첨가한 후 B. subtilis를 접종하여 40일간 수질을 안정화시켰다. 실험결과, 대농갱이의 생존율은 대조구 92.7±3.2%와 BFT 실험구 95.8±3.3%로 조사되었다. 증체율은 대조구 118.1±9.0%와 BFT 실험구 197.7±15.6%을 보였고, 일간 성장율은 대조구 0.87±0.5%, BFT 실험구 1.21±0.06%로 나타났다. 사료효율은 대조구가 43.7±2.6%이었고, BFT 실험구는 70.1±4.1%로 측정되어 BFT 실험구의 사료효율이 더 높은 것으로 조사되었다. 실험기간 동안의 수질 변화를 측정한 결과, pH는 대조구와 BFT 실험구 모두 감소되었고, MLSS는 대조구에서 변화를 보이지 않았지만, BFT 실험구에서는 90일째부터 유의한 증가를 보였다. NH₄⁺-N와 NO₂^--N는 대조구에서 실험 30일째부터 유의한 증가를 보였으나, BFT 실험구에서는 변화를 보이지 않았다. 결론적으로 B. subtilis를 활용한 BFT 시스템을 대농갱이 양성 과정에 적용한 결과, 수질은 안정화 되는 경향을 보였고, 성장도와 사료효율은 대조구에 비해 높은 것으로 조사되어 긍정적인 효과가 있는 것으로 확인되었다.

• 환경생물
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• 제37권4호
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• pp.545-553
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• 2019

도심형 양식시스템을 바이오플락 양식기술과 아쿠아포닉스 배양대로 구성하여 사육수를 교체하지 않고 메기를(사육조 3.3톤 2개) 양성한 결과 151일의 사육 후 2.8 g의 종묘가 평균 무게 171.3 g (총중량 56.53 kg)과 235.5 g (총중량 71.1 kg)로 성장하였다. 입식에서 수확까지의 누적 생존율은 65% 보였고, 성장 구간별로 입식에서 1차 성장 후 분조 이전까지 77.7%, 분조 이후 생존율은 수조에 따라 차이를 보여 각각 92.9%와 78.0%로 나타났다. 초기 바이오플락 사육수가 만들어지는 과정에서 일부 폐사가 발생하였고, 수질이 안정된 이후에는 폐사가 감소하였다. 메기의 혈액분석결과 사육초기 BFT 사육수가 안정화 이전인 4월에 간 손상 지표인 AST의 농도가 유의적으로 높은 값을 보였으며 ALT, triglyceride는 전 사육기간 내에 차이가 없었다. Glucose, cholesterol, total protein은 7월에 유의적으로 높은 값을 보이고 다른 기간에는 차이가 없었다. 메기 사육수를 이용한 아쿠아포닉스 가동 시 생산된 식물은 상추, 바질, 적근대, 적치커리 등이 원활한 성장을 보여 5개월간 총 148.85 kg의 식물을 수확하였다. 또한 아쿠아포닉스 시스템에서 식물재배에 따른 사육수 내의 질산 제거능력과, 질산이 제거된 사육수는 메기 사육수로 재사용이 가능한 것으로 확인되었다. 결론적으로, 본 연구에서는 도심형 양식시스템으로 물을 교환하지 않고 어류를 양식할 때 사육수에 축적된 질산을 제거하고 재사용이 가능한가를 아쿠아포닉스 기술을 결합하여 연구하였으며, 양식생물(메기) 수용량에 따른 적정 식물량을 유지하면 농수산 복합양식이 가능하다는 것을 보여주었다.

• 한국수산과학회지
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• 제43권4호
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• pp.331-339
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• 2010

Shrimp farming is the most important mariculture industry on the west coast of South Korea. However, it has suffered from mass mortality due to viral disease outbreaks and coastal pollution due to water discharge. This study developed an intensive shrimp culture method for outdoor ponds, without water exchange, which minimizes the chance of viral transmission from the environment, reduces coastal pollution by water discharge and enhances shrimp production. A culture trial was conducted in two high-density polyethylene (HDPE)-lined ponds with a $550\;m^2$ surface area. The ponds were stocked with postlarvae of Litopenaeus vannamei, the major farmed shrimp species in Korea, on July 10, 2007, and cultured for 90 days with no water exchange. The stocking density of the postlarvae (B.W. 0.0015 g) was $272\;ind./m^2$, which is eight times higher than in traditional pond culture in Korea. At harvest, the total production of ponds 1 and 2 was 1,362kg ($2.48\;kg/m^2$) and 1,282 kg ($2.33\;kg/m^2$), respectively. This is 20~22 times higher than the mean farmed shrimp production ($0.112\;kg/m^2$) in Korea and about eight times higher than in traditional ponds with a good harvest. Although there was no water exchange throughout the culture period, the mean concentrations of unionized ammonia and nitrite-nitrogen were as low as 0.038 and 6.0 mg/L, respectively. The feed conversion rate (FCR) was 1.38, which is 20~45% lower than that of traditional pond cultures. The high efficiency of the diet in this study is thought to be due to a well-managed feeding strategy and well-developed bioflocs used as diet additions for the shrimp. The final body weight of the shrimp at harvest was low (12.2~12.5 g), compared with that of traditional pond culture. This may have resulted from the combination of a short culture period, high density of shrimp, and low temperature. This study suggests that a super-intensive shrimp pond culture method using biofloc technology with no water exchange can minimize viral transmission via water exchange, reduce coastal pollution, and enhance shrimp production.