• Title/Summary/Keyword: Biochemical test

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Nutrition Survey of Children Attending an Elementary School without a School Lunch Program, in a Low Income Group of Seoul 1. A Study on Anthropometry and Biochemical Test (서울시내 일부 저소득층 비급식 국민학교 아동의 영양실태조사 1. 성장발육상태 및 생화학적 기초조사)

  • 정상진
    • Journal of Nutrition and Health
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    • v.23 no.7
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    • pp.513-520
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    • 1990
  • A nutrition survey of 274 children, 6 to 12 years old, in the Nan Hyang elementary school, which offers no school lunch program and is situated in Shinllim 7-dong of Seoul, as the location of one of the socioeconomically vulnerable groups, was undertaken in July of 1989, to investigate nutritional status. anthropometric data of subjects surveyed slightly lower results than the Korean standards and further lower results than those of Yun Jung elementary school children of Youido, Seoul. 16.3% of subjects were proven to be anemic according to the hematocrit criterion established by the WHO. Mean urinary urea nitrogen/creatinine ration was 9.5$\pm$4.1. Family environment were positively correlated with anthropometric data and results of biochemical tests.

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Comparison of Antimicrobial and Antioxidant Activities by Different Extraction Methods in Korean Bamboos (한국산 대나무의 추출방법에 따른 항균 및 항산화 특성)

  • Choi, Hwan-Seok;Kim, Gwui-Cheol;Shin, Hyun-Jae
    • KSBB Journal
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    • v.27 no.2
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    • pp.131-135
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    • 2012
  • To develop potential cosmetic ingredients with antimicrobial and antioxidant activities of 4 Korean bamboo species (P. bambusoides, P. nigra var. henonis, P. pubescens and Sasa coreana) using three different extraction methods-water, ethanol and supercritical fluid extraction. Antimicrobial activities and DPPH assay have been examined. Among the antimicrobial activities against two test strains, Escherichia coli and Staphylococcus aureus, ethanol extracts of 3 bamboo trees, P. bambusoides, P. nigra var. henonis, and P. pubescens, showed stronger than those of supercritical extracts. However, 4 bamboo supercritical extracts showed dose-dependent increase in antioxidant activity by DPPH assay. These results suggest that water fraction of bamboo extracts may be useful for the cosmetic ingredient with low cytotoxicity.

Acute Oral Toxicity of the Butanol Fraction from Cultured Fruitbody Cordyceps bassiana in Mice (배양 노랑다발동충하초 부탄올분획의 급성경구독성)

  • Park, Eun-Young;Park, Hyung-Jin;Yang, Ki-Sook
    • Korean Journal of Pharmacognosy
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    • v.40 no.3
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    • pp.251-256
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    • 2009
  • Cordyceps bassiana is a parasitic fungus and used as a Chinese traditional medicine. It has been called as DongChungHaCho(summer-plant, winter-worm) in China. Acute oral toxicity was examined in male and female ICR mice. Butanol fraction from Cordyceps bassiana(BuCb) was administered orally at a dose of 2,500 mg/kg, 5,000 mg/kg, 10,000 mg/kg. No death and abnormal clinical signs were observed throughout the administration period. The acute toxicity test on mouse did not show any oversign in net body weight gain, food and water consumptions, organ weights, gross pathological findings by different doses of BuCb. Also, biochemical examination revealed no evidence of specific toxicity. These findings show that BuCb has wide margin of safety on acute toxicity with single exposure.

Studies on the Streptococcus suis isolated from slaughtered pigs (도축돈에서 분리된 Strptococcus suis에 대한 연구)

  • 윤선종;고홍범
    • Korean Journal of Veterinary Service
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    • v.20 no.3
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    • pp.281-288
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    • 1997
  • Streptococcus suis has been identified as a major cause of contagious disease in pigs Ithas been isolated worldwide from pigs suffering from meningitis, bronchopneumonia, polyserositis, polyarthritis and septicemia. Two hundred and fifty-five lung samples of slaughter pigs with gross lung lesions were collected from Jan. to Dec. 1995 in southern Chonnam area. Isolation of S suis were tested by biochemical, serological methods and antimicrobial susceptibility. The results were summerrized as follows ; 1. S suis was Isolated from 30 of 254 pneumonic lungs, 14 Isolates were isolated only, 12 were isolated with p. multocida and 4 were isolated with unidentified Gram positive cocci. 2. In biochemical characteristics studies, all isolates were not grown in 6.5% NaCl medium, and most isolates utilized L-leucine-2-naphtylamide and trehalose, didn't utilize sorbitol, ribose and L-arabinose. 3. In slide agglutination test, 6(20%) Isolates were classified as serotype 2, 4(13.3%) isolates were as serotype l/2, 16, 2 and 2(6.6%) were as serotype 1, 4, 13. 4. S suis isolates showed marked susceptibility to amoxicillin, oxacillin, cephalothin and cepoferazone and high resistance to kanamycin, streptomycin, tetracycline and erythromycin.

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Modeling of Esterase Production from Saccharomyces cerevisiae

  • Thilakavathi, Thilakavathi;Basak, Tanmay;Panda, Tapobrata
    • Journal of Microbiology and Biotechnology
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    • v.18 no.5
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    • pp.889-896
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    • 2008
  • A suitable simple model tested by experiments is required to address complex biological reactions like esterase synthesis by Saccharomyces cerevisiae. Such an approach might be the answer to a proper bioprocessing strategy. In this regard, a logistic model for esterase production from Saccharomyces cerevisiae has been developed, which predicts well the cell mass, the carbon source (glucose) consumption, and the esterase activity. The accuracy of the model has been statistically examined by using the Student's t-test. The parameter sensitivity analysis showed that all five parameters (${\mu}_m$, $K_a$, $X_m$, $Y_{x/s}$, and $Y_{p/x}$) have significant influence on the predicted values of esterase activity.

Properties of Enterococcus faecium isolated from the intestineof slaughting pigs (도축돈 장관에서 분리한 Enterococcus faeciurnab 특성)

  • Kim, In-doc;Ahn, Mi-hyun;Seok, Ho-bong
    • Korean Journal of Veterinary Research
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    • v.44 no.4
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    • pp.581-586
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    • 2004
  • The growth characteristics and the properties of the Enterococcus faecium (E. faecium) isolated from intestine of slaughting pig were examined in order to confirm whether it will be used practically as probiotics or not. E. faecium was identified by morphological and biochemical tests including of final confirming by API strep kit. Among 264 samples, 44 isolates (16.7%) of E. faecium were observed as an exellent growth as a range of $1{\times}10^7CFU/ml{\sim}3.3{\times}10^9CFU/ml$ in viable bacterial count on MRI medium. All isolates were shown non-haemolysis on the blood agar exception of 28 isolates shown ${\alpha}$-haemolysis and were identified as E. faecium by biochemical test using API strep enzyme kits. Eight strains (18.2%) were finally selected from which they were excellent sensitivity in showing of the acid-tolerance and the bile-tolerance in compare with reference strain.

Herbal anti-inflammatory immunomodulators as host modulators in chronic periodontitis patients: a randomised, double-blind, placebo-controlled, clinical trial

  • Deore, Girish D.;Gurav, Abhijit N.;Patil, Rahul;Shete, Abhijeet R.;Naiktari, Ritam S.;Inamdar, Saurabh P.
    • Journal of Periodontal and Implant Science
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    • v.44 no.2
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    • pp.71-78
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    • 2014
  • Purpose: Host modulatory therapy has been proposed as a treatment for periodontal diseases. A class of herbal medicines, known to be immunomodulators, alters the activity of immune function through the regulation of informational molecules such as cytokines. In the current study, we tested the hypothesis that herbal immunomodulator drugs act as an adjuvant to scaling and root planing (SRP) in alleviating periodontal inflammation by improving clinical and biochemical parameters. Methods: Sixty healthy subjects (30 in each group) with moderate and severe chronic periodontitis were enrolled in a double-blind, placebo-controlled, double-masked randomised controlled trial. The control group was treated with SRP and a placebo, whereas the test group was treated with SRP followed by dietary supplementation of Septilin for 3 weeks. Periodontal clinical parameters and serum C-reactive protein (CRP) levels were evaluated for all patients at the baseline, 3 weeks, and 6 weeks. Results: Improved gingival index scores found in the test group as compared to the control group were found to be statistically significant only after 3 weeks (P<0.001). In contrast, the decrease in the sulcus bleeding index and pocket depth scores was statistically highly significant in the test group as compared to the control group after 3 weeks and 6 weeks (P<0.001). However, reduced clinical attachment level and CRP scores, as reflected in the test group as compared to the control group, were not found to be statistically significant after both 3 weeks (P>0.05) and 6 weeks (P>0.05). Conclusions: The results of this clinical-biochemical study suggest that dietary supplementation with herbal immunomodulatory agents may be a promising adjunct to SRP and may aid in improving periodontal treatment outcomes.

Renal failure associated with ingestion of grapes and raisins in dogs (개에서 포도 및 건포도 섭취가 신부전에 미치는 영향)

  • Yuk, Jin-yub;Kim, Cheol-ho;Kim, Tae-yung;Kang, Chung-boo
    • Korean Journal of Veterinary Research
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    • v.45 no.2
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    • pp.287-296
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    • 2005
  • This experiments was designed to observe the effect of period and dose of ingestion of grapes on renal failure in dog. After the dogs had been mixed of general feed, raisins from U.S.A and fresh grapes from Chile selling in Korea. We observed the clinical sign, and analyzed the blood and urine using by biochemical test. The experiment was executed by the two following groups. The experiment group 1. derived renal failure by supplying the mixed general feed with fresh grapes and dry grapes 15 g per weight kg, 2 times a day (AM 09, PM 21), the experiment group 2. executed to derive renal failure by supplying the mixed general feed with fresh grapes and dry grapes 40 g per weight kg, 2 times a day. Extraction of blood for analysis was conducted one time a day and clinical test for renal failure was executed by means of a blood analysis, biochemical analysis, urine analysis, excretory urography (E.U) and E.R.D-$screen^{TM}$ urine test (Heska, USA). The results of group 1 were normal ranges (BUN 9.0~22.6 mg/dl, creatinine 0.8~1.2 mg/dl, Ca 9.7~12.3 mg/dl, Pi 2.9~4.6 mg/dl), renal failure was not observed. On the 3rd day in group 2, azotemia was arisen from the increasing BUN 83 mg/dl (7~25 mg/dl), creatinine 2.3 mg/dl (0.5~1.4 mg/dl), when executed urine was tested by E.R.D-$screen^{TM}$ test using in the early kidney disease diagnosis, microalbumine state was high positive, and it showed stale delay by using excretory urography (EU). This study demonstrated that acute renal failure by grapes and raisins dependent on food dose, and specific characters of individual.

The Trophic State Assessment using Biochemical Composition in the Surface Sediments, the Southern Coast of Korea (표층 퇴적물의 생화학적 조성을 이용한 남해연안 영양상태 평가)

  • Cho, Yoon-Sik;Kim, Yoon-Bin;Lee, Won-Chan;Hong, Sok-Jin;Lee, Suk-Mo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.19 no.2
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    • pp.101-110
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    • 2013
  • In order to classify the trophic state and environmental quality of marine coastal system, an approach using the characteristics and biochemical composition in the sediments can be available. This research, including 25 coastal bay, belong to 131 stations, was carried out along the south coasts of Korea in February 2007. Type of sediment, total ogranic carbon, total nitrogen, phytopigments and biochemical composition(proteins, lipids, carbohydrates) were analyzed. Result from Multi-dimensional Scaling(MDS) ordination indicates that four group can be identified. The result of ANOVA with tukey test shows that the concentrations of proteins, carbohydrates and biopolymeric carbon were significantly different to four groups. We propose the trophic state classification for these groups using the biochemical composition of sediment organic matter. I group(Masan, Jinhae, Haengam) has been defined as hypertrophic state, II group(Tongyeong, Goseong;Jaran, Geoje et al.), as eutrophic; III group(Gamak, Deungnyang, Yeoja et al.), as mesotrophic and IV group(Sinan, Jindo, Muan), as oligotrophic. On the basis of results reported in this study, the biochemical composition of sediment organic matter could be considered an useful and sensitive tool for the classification of the trophic state of marine coastal systems.

Estimation of Ultimate Methane Yields and Biodegradability from Urban Stream Sediments Using BMP Test (BMP(Biochemical Methane Potential) test를 통한 도심하천 퇴적물의 최종메탄발생수율 및 생분해도 산정)

  • Song, Jaehong;Kim, Seogku;Lee, Junki;Koh, Taehoon;Lee, Taeyoon
    • Journal of the Korean GEO-environmental Society
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    • v.11 no.2
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    • pp.33-42
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    • 2010
  • The main objective of this study was to offer informations about the current conditions of stream sediments and to evaluate biochemical methane potentials of stream sediments from the urban streams in Busan city using conventional BMP tests. First we select total 5 urban streams and collect sediment samples. Then, COD, proximate analysis, volatile solid, organic carbon content and elemental analysis were conducted to determine characteristics of the sediments. Results show that COD, volatile solid and organic carbon content are determined in the range of $15.20{\sim}75.07mg\;g^{-1}$, 2.34~11.54% and 1.28~34.21%, respectively. Also, several biochemical methane potential tests were performed in a laboratory. As a result, pH values of the reactors generally increased and then stabilized at 7.11~7.35. In addition, C/N ratio, ultimate methane and carbon dioxide yield (mL/g VS) and biodegradability (%) were determined to 1.05~10.27, 10.1~179.4, 10.3~34.4 and 4.0~30.1, respectively. For the determination of the correlations between ultimate methane yield and ultimate carbon dioxide yield, C/N ratio, COD, volatile solid and organic carbon content, a linear model was fitted to the data using a least-squares algorithm. As a result, except for COD ($r^2=0.7586$) and volatile solid ($r^2=0.7876$), Linear model was well fitted to each data with good values of the correlation coefficient ($r^2=0.9795{\sim}0.9858$). Finally, we propose empirical equations, which contain C/N ratio or TOC, for the prediction of ultimate methane yield for the urban streams in Busan city.