• Title/Summary/Keyword: Biochemical test

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The Biochemical Characteristics of Acetobacter sp. Isolated from Persimmen Vinegar (감식초에서 분리한 Acetobacter sp.의 생화학적 특성)

  • 이상복;전승호
    • Journal of environmental and Sanitary engineering
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    • v.14 no.4
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    • pp.180-187
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    • 1999
  • The following is experimental result of Biochemical characteristics of Acetobacter sp. Acetobacter sp. were gram negative, short rod, non-spore-forming and motile. It reacted positively catalase, methyl red, oxidation fementation, voges-proskauer and nitrate reduction tests and hydrogen sulfide test negative and ONPG negative. Acetobacter sp. showed normal growth curve in Carr broth and there was no significant difference between isolates and type strains such as Acetobacter aceti(KCTC 1010), Acetobacter diazotrophicus(KCTC 2859). Acetobacter liquefaciens(KCTC 2804) and Acetobacter pasteurianus(KCTC 1008).

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Comparison analysis of psychological and biochemical indices of chronic life stress

  • Kim, Dongsoo;Park, Se-kwon;Chung, Yeon-Soo;Moon, Soo-Jong
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2003.10b
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    • pp.162-162
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    • 2003
  • The psychological and biochemical stress responses of healthy men and women were measured under the normal situation. Experimental subjects, who were cadets of the Korea Air Force Academy, were selected by the random sampling. Quantitative scaling of the psychological stress was obtained by life stress test(Cheon et al, 2000) and Minnesota Multiphasic Personality Inventory(MMPI).(omitted)

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Biochemical property identification of 10 strains of Bacillus thuringiensis and 10 strains of Bacillus cereus (7 strains of non-emetic and 3 strains of emetic type) by API test

  • Hong, Yong-Gun;Lee, Jin-Joo;Kwon, Seung-Wook;Kim, Sang-Soon
    • Korean Journal of Food Science and Technology
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    • v.52 no.6
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    • pp.678-684
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    • 2020
  • The objective of this study was to identify the fermentation characteristics of Bacillus thuringiensis and emetic, non-emetic Bacillus cereus using analytical profile index (API) test. Ten strains of B. thuringiensis and 10 strains of B. cereus including 3 strains of emetic type were used at the same concentrations. The differences of fermentation characteristics between the B. thuringiensis and B. cereus was not obvious, but the differences between the non-emetic and emetic B. cereus were distinctive. Seven among 50 substrates were negative for all non-emetic B. cereus strains and positive for all emetic strains, and three substrates among additional 12 substrates had the same tendency. From these differences, 3 emetic B. cereus strains were not indicated as B. cereus by API test. These results indicate that API test is not a suitable method to identify some strains of emetic B. cereus, and the distinctive differences in substrate utilization can be used to improve selective media.

Isolation and Biochemical Properties of Pasteurella multocida from the pneumonic lungs of swine in Cheju (제주지방 돼지의 폐병변으로부터 Pasteurella multocida 분리 및 생화학적 특성)

  • 김옥녀;이두식;문호규;김우택;서문현;배종희;임윤규;조길재
    • Korean Journal of Veterinary Service
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    • v.18 no.2
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    • pp.124-132
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    • 1995
  • The present study was conducted to investigate the incidence of Pasteurella multocida infection in cheju swine from March 1994 to December 1994 isolated organisms were identified by the biochemical properties, cellulose serological type and antibiotic susceptibilities. Pasteurella multocida was isolated from the Lungs of 96 pigs with pneumonia(51 %) among 188 slaughtered pigs. The majority of p multocida isolates were identical to those of the standard strains. On the classification of the capsular type of the isolated p multocida it consist of the 88 isolates of type A(91.6%) 2 isolates of type D (2%) and un classified 6 types(6.2%). The majority of the 96 isolates of p multocida highly susceptible to the antibiotics including ampicillin(Am), cephalotin(Ce), erythromycin(Em), gentamycin(Gm), kanamycin(Km), lincomycin(Lm), neomycin(Nm), penicillin(Pc), streptomycin(Sm), solfametoxazol/trimethoprim(Sxt) and tetracycline(Te)

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Analysis on the Findings of Serum Biochemical Test in Inhabitants Infected with Clonorchis sinensis

  • Sohn, Woon-Mok;Cho, Young-Pyo;Kim, Kee-Jin;Kim, Mi-Young;Lee, Chong-Wook;Park, Do-Hum
    • Biomedical Science Letters
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    • v.7 no.1
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    • pp.39-45
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    • 2001
  • The findings of serum biochemical tests in the inhabitants residing in some riverside areas of kyongsangnam-do were analized to know the correlation with the Clonorchis sinensis infection. A total of 2,722 fecal samples of inhabitants was examined by Kato's cellophane thick smear and formalin-ether sedimentation methods, and their serums were analized biochemically. Three hundred and thirty-three inhabitants (12.2%) were positive with C. sinensis eggs (egg-positive group), and remaining 2,389 were negative (egg-negative group). In the egg-positive group, the positive .ate of aspartate aminotransferase (AST) was 6.9% (male: 10.3%; female: 3.2%), that of alanine aminotransferase (ALT) was 6.3% (male: 9.1%; female: 3.2%), of $\gamma$-glutamy1 transpeptidase ($\gamma$-GIP) was 9.0% (male: 13.1%; female: 4.4%), and that of $\alpha$-fetoprotein (AEP) was 3.0% (male: 2.9%; female: 3.2%). In the egg-negative group, the positive rate of AST was 5.2% (male: 8.9%; female: 3.2%), that of ALT was 5.1% (male: 8.6%; female: 3.2%), of $\gamma$-GTP was 7.5% (male: 13.0%; female: 4.5%), and that of AFP was 0.3% (male: 0.5%; female: 0.1%). In the egg-positive group, 127 cases (38.1%) were light infection, 108 (32.4%) were moderate infection,64 (19.2%) were heavy infection, and 34 (10.2%) were very heavy infection. The positive rates of all tests were high according to the intensity of infection. From the above results, It was confirmed that prevalence of clonorchiasis is still high in some riverside areas of Kyongsangnam-do, and findings of serum biochemical tests are not different between the egg-positive group and egg-negative group. However, there were some difference in the positive rates of serum AST, ALT, $\gamma$-GTP and AEP by the age, sex and the intensity of infection.

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Nano-capsulation of L-Ascorbic Acid in Nonaqueous System (L-Ascorbic Acid의 비수계 나노 캡슐화)

  • Hong, Joo-Hee;Song, Ki-Se;Kim, Kyoung-Jun;Lee, Chae-Seong;An, Byeong-Min;Kim, Byoung-Sik
    • Applied Chemistry for Engineering
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    • v.19 no.6
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    • pp.604-608
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    • 2008
  • In this work, the basic research for nano-capsulation of L-ascorbic acid (Vitamin C) in nonaqueous system was carried out. 500 nm-sized nano-capsules were prepared in nonaqueous system, and the emulsified capsule had mean size of 410 nm. The stability test on the temperature and the storage periods was performed at 4, 20, and $30^{\circ}C$ for 30 days. After 5 days, L-ascorbic acid was extricated 5.1, 9.3, and 12.5% at each temperature, but only 1~2 % was extricated after the time span. Likewise, the results of the skin susceptibility on women and men, each 10 persons, revealed that the very thin allergy was shown from only a woman after 2 days, but it was not shown from the others.

Pattern of antimicrobial resistance and biochemical characteristics of Salmonella Typhimurium isolated from diseased pigs in Gyeongbuk province (경북지방 환돈에서 분리한 Salmonella Typhimurium의 생화학적 성상 및 약제내성 패턴)

  • Kim, Seong-Guk;Eom, Hyun-Jung;Kim, Soon-Tae;Jang, Young-Sul;Jo, Min-Hee
    • Korean Journal of Veterinary Service
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    • v.33 no.1
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    • pp.51-57
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    • 2010
  • Salmonella Typhimurium is a virulent pathogen for human and animal. We studied serotypes, biochemical characteristics, and antimicrobial resistance of S. Typhimurium isolated from diseased pigs in Gyeongbuk province over 1998 to 2008. One hundred sixteen isolates were identified as S. Typhimurium by biochemical characteristics and serotypes from 90 farms. The biochemical characteristics of S. Typhimurium isolates was production of $H_2S$, indole-negative, fermentation of mannitol, dulcitol, sorbitol, inositol, rhamnose, and maltose, and ornithine decarboxylase. At antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, cefepime, ciprofloxacin, while were highly resistant streptomycin, cephalothin, enrofloxacin, nalidixic acid, apramycin, chloramphenicol, tetracycline. The isolates were divided into 65 resistant patterns and 47 of the isolates were shown as a DT104 ASSSuT resistant phenotype.

Isolation and Characterization of Flavobacterium johnsoniae from Farmed Rainbow Trout Oncorhynchus mykiss

  • Suebsing, Rungkarn;Kim, Jeong-Ho
    • Fisheries and Aquatic Sciences
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    • v.15 no.1
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    • pp.83-89
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    • 2012
  • Flavobacterium johnsoniae was isolated from farmed rainbow trout Oncorhynchus mykiss in Korea, and its biochemical and molecular characterization was determined. Yellow-pigmented bacterial colonies were isolated from 18 of 64 fish samples (28.1%) on trypticase soy agar plates, and their biochemical profiles were characterized by API 20E and API 20NE test kits. F. johnsoniae was identified by biochemical phenotyping of factors including rapid gliding motility, Gram-negative condition, oxidase- and catalase-positive status, Congo red absorption, nitrate reduction, ${\beta}$-galactosidase production, acid production from glucose, and gelatin and casein hydrolysis. PCR and subsequent sequencing of 16S rRNA confirmed that the yellow-pigmented colonies were most similar to F. johnsoniae. The alignment analysis of 16S rRNA sequences also showed that all 18 rainbow trout isolates had highly similar homologies (97-99% identity). One isolate was selected and named FjRt09. This isolate showed 98% homology with previously reported F. johnsoniae isolates, and in phylogenetic analysis was more closely grouped with F. johnsoniae than with F. psychrophilum, F. columnare, or F. branchiophilum. This is the first report on the occurrence and biochemical characterization of F. johnsoniae isolated from rainbow trout in Korea.

Biochemical characteristics, antimicrobial susceptibility of Salmonella Gallinarum detection in chickens and rapid diagnosis by polymerase chain reaction (닭에서 분리한 Salmonella Gallinarum의 생화학적 특성, 약제감수성과 polymerase chain reaction을 이용한 신속진단)

  • Chu, Keurn-Suk;Lee, Jeong-Won;Song, Hee-Jong
    • Korean Journal of Veterinary Service
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    • v.32 no.1
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    • pp.43-48
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    • 2009
  • Salmonella enterica serovar Gallinarum is the causative agent of fowl typhoid (FT) and Salmonella enterica serovar Pullorum is pullorum disease (PD), a severe systemic disease of chick and it has the same antigenic fomula, the close relation but distinct pathogen. The traditional bacteriologic and serologic methods routinely used but tedious, time consuming. some of biochemical differences are helpful in differentiating the two organisms, however variation in the characteristics of some strains can be observed. During 2006 to 2008, there was isolated 30 strains. The biochemical characteristics of S. Gallinarum was nonmotile, fermentation of dulcitol, maltose but positive arginine (6.6%), lysine (83.3%) and arabinose (20.0%). The antimicrobial susceptibility test showed 100% sensitive to amikacin, ampicillin, amoxicillin/clavulanic acid and florfenicol, but resistant to penicillin (100%) and erythromycin (60.0%). This PCR method can be applied in the diagnosis between S. Gallinarum and S. Pullorum.

Genetical and Biochemical Studies on White Egg Strains of Silkworm, Bombyx mori (누에 백란 계통의 유전적 특성구명을 위한 유전.생화적 연구)

  • 이은정;임봉학
    • Journal of Sericultural and Entomological Science
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    • v.39 no.2
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    • pp.134-139
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    • 1997
  • To elucidate genetical and biochemical characters of the white egg strains preserved in National Sericultural and Entomology Research Institute(NSERI), RDA of Korea, the genetic segregation ratios in egg colours were investigated by crossing test, and egg pigment precursors were also analyzed by paper chromatography and UV-spectrophotometer scanning. The result obtained by crossing test between the white egg strains and normal one illustrated that the most of white egg strains showed typical segregation ratio of white egg-2(w-2), while maternal inheritance which can be seen in white egg-1(w-1) was not found in any white egg strains. Paper chromatographic analysis showed that egg extracts of all the white egg strains contained 3OH-kynurenine, while kynurenine which is known to existed in white egg-1(w-1) could not be detected at all. From the results of these experiments, it was found that all the white egg strains preserved in NSERI were classified as the white egg-2(w-2) strain.

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