• Title/Summary/Keyword: Biochemical test

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Facilitated Oxygen Transport through a Polyethersulfone Membrane Containing Cobalt Tetraphenylporphyrin-Benzylimidazole (Cobalt Tetraphenylporphyrin-benzylimidazole을 포함한 산소 촉진수송막)

  • Lee, Seung Hwan;Park, Se Hyung;Park, Jung Hoon
    • Membrane Journal
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    • v.28 no.6
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    • pp.424-431
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    • 2018
  • The gas separation performance of a mixed membrane structure based on a mixture of polyethersulfone (PES) and cobalt tetraphenylporphyrin-benzylimidazole (CoTpp-BIm) as an oxygen carrier was investigated. The CoTpp-BIm mixed PES membrane had an asymmetric structure with a mixture of finger structure and sponge-like structure, and the upper surface was dense. The gas separation performance test was carried out using $94%\;N_2$ gas and $6%\;O_2$ mixed gas. Oxygen and nitrogen permeability coefficients were measured at ${\Delta}P$ ranging from 15 to 228 cmHg and the permeate side of the PES membrane was maintained at vacuum level. The oxygen permeability coefficient of CoTpp-BIm mixed PES membranes increased as supplied pressure was decreased. When the supply pressure was 15 cmHg, the gas permeability ($P_{O_2}$) was 6676 Barrer, the $O_2/N_2$ selectivity (${\alpha}$) was 6.1, and the promoting factor (F) was 2.39. Based on these results, it was confirmed that the addition of CoTpp-BIm to the PES film improved the oxygen separation characteristics.

S-(N,N-Diallyldithiocarbamoyl)-N-acetylcysteine: Synthesis and Biochemical Properties Associated with Chemoprevention (S-(N,N-Diallyldithiocarbamoyl)-N-acetylcysteine의 합성 및 발암억제와 관련된 생화학적 특성)

  • 이병훈
    • Toxicological Research
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    • v.14 no.2
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    • pp.177-181
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    • 1998
  • Dithiocarbamate and mixed disulfide containing allyl functions were designed and synthesized as putative chemopreventive agents, i.e. N,N-diallyldithiocarbamate (DATC) and S-(N,N-diallyldithiocarbamoyl)-N-acetylcysteine (AC-DATC). DATC and AC-DATC were administered and the activities of cytosolic glutathione S-transferase (GST), glutathione reductase (GR) and microsomal N-nitrosodiethylamine (NDEA) deethylase were assayed in order to test the effects of these organosulfur com-pounds on the detoxification and metabolic activation system of NDEA. The amounts of hepatic glutathione (GSH and GSSG) was also determined. The administration of DATC to rats led to an increase in the activity of GR and to an inhibition of CYP2E1-mediated NDEA deethylation. AC-DATC induced the activity of GR and GST, increased the hepatic GSH content and inhibited the rate of NDEA deethylation. The level of GSSG was decreased as a consequence of the increased activity of GR. These effects may contribute to possible antimutagenic and anticarcinogenic action of the dithiocarbamates investigated.

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Development of Dietary Supplement for the Growth and Exercise Performance Improvement (성장과 운동수행능력 향상에 도움을 주는 식이조성물의 개발)

  • 윤승원;정은희;양동식;이홍석;윤유식
    • Korean Journal of Community Nutrition
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    • v.8 no.3
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    • pp.349-355
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    • 2003
  • We developed a dietary supplement using natural herbs and nutrients for the growth and exercise performance improvement. It called 50-22 supplement containing distilled extracts of natural herbs to remove bitterness and the addition of Vitamin B1, B6, Ca and xylitol. We investigated on the effect of 10-22 supplementation on the maximal exercise performance, IGF-1 (insulin like growth factor-1) concentration and antioxidant activity in SD rat model. In result, JR-22 supplement group was better than control group about 10% in exercise performance test and increased about 63% of IGF-1 concentration in blood. In addition, the oxidative damage induced by exercise was reduced by JR-22 supplementation. Therefore, we suggested that 50-22 supplementation enhanced effectively exercise performance and IGF-1 concentration and reduced to oxidative stress in muscles. Also, we analysed biochemical factors in blood for the safety of JR-22 supplement. We known that there is no change of blood lactic acid, ammonia, inorganic phosphorous ion and creatine kinase activity. (Korean J Community Nutrition 8(3) : 349∼355, 2003)

Studies on Campylobacter jejuni and Campylobacter coli contamination on broiler carcasses in slaughterhouse (도계장 도계의 Campylobacter 균 오염에 관한 연구)

  • Na, Ho-Myung;Koh, Ba-Ra-Da;Park, Seong-Do;Kim, Yong-Hwan
    • Korean Journal of Veterinary Service
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    • v.30 no.1
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    • pp.77-84
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    • 2007
  • The present study was carried out to investigate the incidence of Campylobacter spp. from the chicken carcasses in slaughterhouse. A total of 9 strains were primarily isolated from enrichment culture and selective culture of the sample with candle and microaerophilic chamber method. Nine of Gram-negative, catalase-positive and oxidase-positive strains were further isolated by the determination of biochemical characteristics and finally identified as Campylobacter jejuni with HIP 400F and HIP l134R primers. Therefore, this PCR method proved to be useful as a routine diagnostic test for the Campylobacter detection and confirmation of C. jejuni and C. coli in naturally contaminated poultry samples.

Rhodococcus equi pneumonia in foals in Gyeonggi-do and characterization of the isolates from lesions and environment

  • Kim, Sue-Jung;Yook, Sim-Yong;Hwang, Jun-Suk;You, Myung-Jo;Jun, Moo-Hyung
    • Korean Journal of Veterinary Research
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    • v.48 no.2
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    • pp.139-143
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    • 2008
  • We report here two cases of Rhodococcus (R.) equi-causing pneumonia of Throughbred foals in Gyeonggi-do in 2006. R. equi was isolated from the lung lesions of the dead foals, and from the feces and soils on the farms where the clinical cases of R. equi infection occurred. The isolates were characterized by biochemical properties, polymerase chain reaction for vapA gene and antimicrobial susceptibility. In drug susceptibility test, erythromycin, gentamycin, vancomycin, and rifampin were found to be the most susceptible for all isolates. These results suggest that R. equi pneumonia may be endemic in the horse-breeding farms in inland Korea and the farm environment may be widely contaminated with virulent R. equi.

1차원 현장 soil column 실험을 통한 SAT 반응 모델 검증

  • ;Jeongkon Kim
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.83-86
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    • 2003
  • Soil Aquifer Treatment (SAT) is a technique in which secondary- or tertiary-treated wastewater is infiltrated through unsaturated soil and stored in the saturated zone. In SAT, contaminants are removed by physical and biochemical reactions taking place in soils. In this study, a numerical model was developed to predict changes in water quality during SAT operations. The contaminant species considered in the model were ammonium, nitrate, dissolved organic carbon, and dissolved oxygen. The model was calibrated against experimental data obtained from one dimensional soil column tests conducted for 84 days. The calibrated model will be used to find out optimum conditions for the pilot- and regional-scale SAT operations to be scheduled for the next phase of this project.

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Effects of Antioxidants on UV-B Susceptibility in Soybean (항산화 물질이 UV-B에 대한 콩의 감수성에 미치는 영향)

  • 김학윤;박이상;이인중;신동현;김길웅
    • Journal of Environmental Science International
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    • v.7 no.5
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    • pp.633-638
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    • 1998
  • To determine whether the enhanced UV-B causes oxidative stress, and to test the relationship between plant growth response and biochemical defense response to UV-B, two soybean plants, Keunolkong, a highly UV-B susceptible cultivar, and Danyeubkong, a less UV-B susceptible cultivar, were subjected to the enhanced UV-B [daily dose : 0.06 (control) and 11.32 (enhanced UV-B) kJ $m^{-2}$ ; $UV-B_{BE}$] for 3 weeks. Contents of malondialdehyde and total carotenold were increased in Keunolkong compared with Danyeubkong by UV-B. In control plants, ascorbate level of Danyeubkong was 3 times higher than that of Keunolkong. The ratio of dehydroascorbate/ascorbate was highly increased in Keunolkong by UV-B . The activities of antioxidative enzyme such as superoxide dismutase, ascorbate peroxidase, monodehydroascorbate reductase and glutathione reductase were increased in both cultivars by UV-B. This results indicate that enhanced UV-B caused oxidative stress in both two cultivars, especially in Keunolkong. Susceptibility of two soybean cultivars to UV-B is closely related to the levels of antioxidants such as carotenoid and ascorbate.

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Characterization of Mosquitocidal Bacillus thuringiensis Strain H9B (모기유층에 대한 살충성 Bacillus thuringiensis H9B 균주의 특성)

  • 이기희;김광현;김병우
    • Microbiology and Biotechnology Letters
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    • v.21 no.5
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    • pp.393-398
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    • 1993
  • One strain of mosquitocidal Bacillus thuringiensis, H9B, was isolated from soil. The biochemical characteristics and flagella antigenicity of the strain H9B is similar to that of B. thuringiensis subsp. darmstadiensis. The delta-endotoxin of the strain H9B coincided with that of B. thuringiensis subsp. darmstadiensis strain 73E10-2 on agarose double immunodiffusion test. The delta-endotoxin of B. thuringiensis subsp. israelensis contains hemolysin fragment (28 kb) on SDS-PAGE when the delta-endotoxin was solubilized in alkali, while that of the strain H9B does not contain 28 kb protein.

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Serogroup and Drug Susceptibility of Pasteurella Mutocida Pneumonia in Pig (돈 폐염 유래 Pasteurella Multocida 혈청형 및 약제 감수성)

  • 오강희;박노찬;김이준;박덕상
    • Korean Journal of Veterinary Service
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    • v.13 no.1
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    • pp.69-74
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    • 1990
  • The present study was conducted to investigate the incidence of pasteurella multocida infection in kyungbuk swine herds during the period from July 1989 to November 1989 and some properties of the isolated organisms. P. multocida was isolated from lungs of 155 slaughtered pigs, 43(27.7%) pigs were culture positive. The majority of biochemical and cultural properties of the P. multocida isolates were identical to those of the standard strains. The capsular serogroups and drug susceptibility of 43 isolates of P. multocida from pigs with pneumonic lesions were investigated. P. multocida isolates were typed for capsular serogroupes A by hyaluronidase inhibition of capsule and D by acriflavin auto agglutination. Most isolates(60.4%) were type A, 18.6% were type D, and the remaining 21.0% were untypable. In antimicrobial susceptibility test these isolates of P. multocida were susceptible in order of ampicillin (86.0%), trimethoprim sulfamethoxazole(83.7%), colistin(81.4%), chloramphenicol(79.1%), but the majority of them were resistant in order of streptomycin(30.2%), triple sulfa (4.6%).

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Coincident finding of bronchopneumonia by Pasteurella multocida, Bordetella bronchiseptica and Klebsiellosis in muskrat (사향쥐에서 Pasteurella multocida 및 Bordetella bronchiseptica에 의한 bronchopneumonia와 Klebsiellosis의 동시감염 증례)

  • Park, Mi-Young;Jeon, Oh-Sook;Cho, Yun-Kyoung;Choi, Kyung-Mook;Woo, Jong-Tae
    • Korean Journal of Veterinary Service
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    • v.32 no.2
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    • pp.165-169
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    • 2009
  • Suppuratives bronchopneumonia was found in a 3-month old domestic muskrat (Ondatra zibethicus). Dead muskrat showed hemorrhagic nasal discharge, severe hemorrhage and consolidation were observed in the lungs in necropsy. Histologically, severe polymorphic neutrophils and alveolar macrophages were infiltrated in the bronchus, bronchioles, alveoli. P. multocida and B. bronchiseptica were identified from the lungs, Klebsiella was isolated from the cecum. We demonstrated those organisms by biochemical test and confirmed P. multocida capsular type A by means of polymerase chain reaction (PCR).