• Journal of fish pathology
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• v.5 no.1
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• pp.29-35
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• 1992

This study was carried out in order to identify the biochemical and serological characteristics of Edwardsiella tarda isolated from cultured flounder, Paralichthys olivaceus in the east and south coast of Korea. During the year of 1990 and 1991, the number of isolated E. tarda were 131 strains. To identify the biochemical characteristics of them kinds of tests were conducted. The results represented that all the strains had the same biochemical characteristics, and their biochemical characteristics were no differences among strains. A serological analysis was carried out based on agglutination test with antiserum belonging to E. tarda serotype a (E-22), b (SU-138), c (SU-100) classfied in japan. The selected 10 isolates showed agglutinin titer of 5120-2560, 40-80 and below 40 against E. tarda serotype a, b and c, respectively. Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) profiles of cell proteins of selected 10 isolates were showed no differences in kinds and volumes of proteins among strains.

• Journal of Life Science
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• v.20 no.10
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• pp.1549-1555
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• 2010

Streptococcus suis is a worldwide pathogen of a variety of porcine infection and has also been described as a pathogen for humans. We studied biochemical characteristics, antimicrobial susceptibility, and identification of polymerase chain reaction (PCR) of S. suis isolated from diseased pigs in Gyeongbuk province from 2004 to 2009. Sixty-one isolates were identified as S. suis by biochemical characteristics and PCR from 40 farms. The biochemical characteristics of S. suis isolates were production of VP-negative, hippurate, esculin, and arginine decarboxylase-positive, and fermentation of carbohydrate was variable lactose, trehalose, inulin, and raffinose, which was typeable 11 phenotype. In an antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, ampicillin, cephalothin, cefoperazone and florfenicol, while being highly resistant to streptomycin, kanamycin, amikacin, neomycin, erythromycin, clindamycin, and tetracycline. The isolates were divided into 11 phenotypes of biochemistry. By using PCR, the 16S-rRNA gene DNA fragment was detected at 304 bp from all of isolates. These results may provide the basic information needed to establish strategies for the prevention of S. suis infection in pigs.

• Applied Chemistry for Engineering
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• v.34 no.3
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• pp.264-271
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• 2023

In this study, ASCO^Ⓡ SLES-430 surfactant was synthesized by adducting 3 moles of ethylene oxide and 1 mole of propylene oxide to lauryl alcohol followed by a sulfation process, and the structure of the synthesized ASCO^Ⓡ SLES-430 was elucidated by performing FT-IR, ¹H-NMR and ¹³C-NMR analyses. Interfacial properties such as critical micelle concentration, static surface tension, emulsification index, and contact angle were measured, and environmental compatibility indices such as oral toxicity and skin irritation were also estimated for ASCO^Ⓡ SLES-430. Both results were compared with ASCO^Ⓡ SLES-226 and ASCO^Ⓡ SLES-328 SLES surfactants possessing 2 moles and 3 moles of ethylene oxide, respectively. In particular, both foaming ability and foam stability were evaluated for ASCO^Ⓡ SLES-430 and compared with ASCO^Ⓡ SLES-226 and ASCO^Ⓡ SLES-328, which have been widely used in detergent products, in order to test the potential applicability of ASCO^Ⓡ SLES-430 in detergent product formulation for a small capacity built-in washing machine.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.180-180
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• 2022

최근 하천 횡단구조물로 형성된 호소 및 저수지에는 오랜 시간동안 다양한 유기 퇴적물이 유입되고 오염되어 호소환경 및 호소 생태계에 악영향을 미칠 수 있으며, 환경 여건에 따라 메탄과 온실가스를 유발할 수도 있다. 이러한 온실가스를 검출하고 그 원인을 파악하여 감축하는 일련의 기술이 국가적인 온실가스 감축 정책과 맞물려 새로운 이슈로 등장하였다. 일반적으로 메탄가스를 측정하는 실험은 BMP(Biochemical Methane Potential)-Test이며, 수치모의에 비해 BMP-Test는 수심이 깊은 지역이나 유속이 빠른 구역에는 적용이 어려운 한계가 존재한다. 이러한 BMP-Test의 한계점을 보완하는 차원에서 사전검증된 수치모형 COMSOL Multiphysics 소프트웨어를 이용하여 메탄가스 발생기작을 재현 및 도출하고 실내 실험결과와 비교 분석하였다. 첫째, COMSOL Multiphysics가 호소에서 발생하는 메탄가스 발생기작의 재현 타당성을 입증하기 위해 동일한 조건의 인공합성된 유기물을 이용한 실내 실험에서 수행된 BMP-Test와 비교·분석을 수행하였다. 둘째, COMSOL Multiphysics에 TOC(총유기탄소), TP(총 인)에 따른 유기물 조건과 메탄 발생 화학식을 설정하여 온도에 따른 메탄 생성량과 반응상수를 산출하였고, 이를 BMP-Test 결과와 비교하였다. BMP-Test의 비교·분석 결과를 바탕으로 하천 및 호소에서 발생하는 메탄가스 발생에 대한 COMSOL Multiphysics의 활용가능성을 검증하였다. 향후 하천 및 호소로부터의 발생가능한 온실가스 감축 목표를 달성하기 위한 하천 유기물 환경의 평가 또는 최적화 조성에 유사한 검증과정을 거친 COMSOL Multiphysics를 활용할 수 있을 거라 기대된다.

• YAKHAK HOEJI
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• v.38 no.1
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• pp.46-56
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• 1994

Daily oral administration to Sprague Dawly rats for 4 weeks of DWP-301, at doses of 0, 500, 1000, 2000 mg/kg presented following results; 1) No animals died and there were no significant differences in general signs, body weight, food consumption, urinalysis haematological, biochemical, gross pathological and histopathological examination between control and treated rats. 2) Water consumption, pH-, protein- urobilinogen-, ketone-values in urine were significantly increased in the treated male and female rats. It is supposed that these differences in animals are a consistent feature of repeated overdosage with test suspensions. The results indicate that the non toxic dose of test compounds in rats is over 2000 mg/kg in this test system.

• Journal of Veterinary Clinics
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• v.14 no.1
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• pp.37-41
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• 1997

For the inspection of the occurrence situation of porcine pleuropneumonia and serotyping of Actinobacillus pleuropneumoniae strains isolated from lung lesions of pig in Korea, a series of experimentation have been carried out by the isolation and identification of A pleuropneumoniae, serotyping by coagglutination test, observation of lung lesion and clinical signs from 360 cases of porcine pneumonia in Clinical Pathology Laboratory, Bayer Veterinary Medical Research Institute. The results could be summarized as follows. The reaction of coagglutination between the reference antigens and the specific reagents of A pleuropneumoniae was strongly agglutinatied within 30 seconds without cross reaction. The 89 strains of A pleuropneumoniae were isolated from 360 cases of porcine pleuropneumonis and the biochemical properties of the isolates were same as the reference strains. The 89 isolated strains could be serotyped 39 strains as setotype 5, 34 strains as serotype 2, 8 strains as serotype 3, 2 strains as serotype 7 by coagglutination test, respectively. The clinical signs of pleuropneumonia were weakness, fever, anorexia, dyspnea and laboured breath in the later stages. The gross lesions of lung were haemorrhages, enlargement of interlobular septa, nodular formation and adhesion of the pleura.

• Korean Journal of Veterinary Service
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• v.22 no.2
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• pp.151-158
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• 1999

This study was conducted to investigate the biochemical characteristics, the population and antibiotic susceptibility test of Clostridium perfringens isolated from intestinal contents of slaughter cattle in Kyung-ju and Po-hang. 1. In slaughter cattle Cl perfringens were isolated from intestinal contents of 51 of 101 cases(50.4%) and the population were $\leq$$10^5$cfu/ml of 44 cases(86.3%). 2. In antibiotic susceptibility test, ampicillin, bacitracin, cephalothin, penicillin polymyxin B were highly susceptible, ohloramphenicol, erythromycin, tetracycline were lowly susceptible, gentamicin, kanamycin, amikacin, streptomycin, sulfamethoxine, sulfamethazine were resistant. 3. In leaving test intestinal contents leaved for 0, 4, 8, 16, 32 hours in room temperature and population of Cl perfringens were gradually increased.

• Journal of the korean veterinary medical association
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• v.25 no.7
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• pp.423-433
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• 1989

The bovine mastitis investigated with 910 cows of 44 dairy farms in the area of Seoul and Kyunggi-Do. The streptococcal species were isolated from milk with mastits and their biochemical properties and serological reaction were investigated, and an antimi

• Environmental Analysis Health and Toxicology
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• v.11 no.3_4
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• pp.1-10
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• 1996

The purpose of this study was to characterise the subacute toxic potency of i.v. administered KHPC-005 and 006. Few test compounds-related toxic effects were observed in body weight gain, clinical signs, urinalysis, hematological parameters and serum biochemical values. Gross necropsy and histopathology revealed no evidance specific toxicity. Our data indicated that no-observed effect level of KI-IPC-005 and 006 were estimated to be 10mg/kg and 4mg/kg in male rats, and 10mg/kg and 1.33mg/kg in female rats, respectively.

• Membrane Journal
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• v.11 no.3
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• pp.97-108
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• 2001

Bipolar membranes have gained increasing interest as a tool for an efficient production of acids and bases from the corresponding salts. A large number of potential applications have been identified in the chemical and biochemical industry have been identified and evaluated on a laboratory scale. In spite of extensive research efforts and laboratory and pilot plat test, there are hardly any large-scale commercial plants. In this paper the basic concept of the electrodialytic water dissociation with bipolar membranes are briefly reviewed. The limitations of today\`s bipolar membranes are indicated. Selected examples of successful applications of the bipolar membrane technology are discussed.