Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antimicrobial Susceptibility and Biochemical Characteristics of Streptococcus suis Isolated from Diseased Pigs in Gyeongbuk Province

경북지방 환돈에서 분리한 Streptococcus suis의 생화학적 성상 및 약제감수성

  • 최성균 (경상북도 가축위생시험소) ;
  • 김성국 (경상북도 가축위생시험소) ;
  • 김영환 (경상북도 가축위생시험소) ;
  • 최정혜 (경상북도 가축위생시험소) ;
  • 조민희 (경상북도 가축위생시험소) ;
  • 조길재 (경북대학교 수의과대학)
  • Received : 2010.08.18
  • Accepted : 2010.10.04
  • Published : 2010.10.30
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Abstract

Streptococcus suis is a worldwide pathogen of a variety of porcine infection and has also been described as a pathogen for humans. We studied biochemical characteristics, antimicrobial susceptibility, and identification of polymerase chain reaction (PCR) of S. suis isolated from diseased pigs in Gyeongbuk province from 2004 to 2009. Sixty-one isolates were identified as S. suis by biochemical characteristics and PCR from 40 farms. The biochemical characteristics of S. suis isolates were production of VP-negative, hippurate, esculin, and arginine decarboxylase-positive, and fermentation of carbohydrate was variable lactose, trehalose, inulin, and raffinose, which was typeable 11 phenotype. In an antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, ampicillin, cephalothin, cefoperazone and florfenicol, while being highly resistant to streptomycin, kanamycin, amikacin, neomycin, erythromycin, clindamycin, and tetracycline. The isolates were divided into 11 phenotypes of biochemistry. By using PCR, the 16S-rRNA gene DNA fragment was detected at 304 bp from all of isolates. These results may provide the basic information needed to establish strategies for the prevention of S. suis infection in pigs.

Streptococcus suis는 사람과 돼지에서 다양한 감염을 유발하는 병원체이다. 2004년부터 2009년까지 경북지방 양돈장에서 병성감정 의뢰한 돼지 가검물을 대상으로 S. suis를 분리하여 생화학적 성상, 약제감수성 시험 및 PCR을 이용한 동정을 실시한 결과는 다음과 같다. 40개 농장에서 분리한 61주의 S. suis에 대하여 생화학적 성상시험 결과 모두 S. suis로 동정되었으며, VP 음성, hippurate, esculin, arginine decarboxylase 양성, lactose 분해능이 있는 것으로 나타났다. Trehalose와 starch 분해능은 비교적 높게 나타났으며 생화학적 성상에 따른 phenotype은 11형으로 나누어졌다. 항생제 감수성 시험 결과 대부분의 균주가 amoxicillin/clavulanic acid, ampicillin, cephalothin, cefoperazone, florfenicol에 높은 감수성을 나타내었지만 amikacin, kanamycin, neomycin, streptomycin, erythromycin, clindamycin, tetracycline에는 높은 내성을 나타내었다. 16S-rRNA 특이유전자 부위를 이용한 PCR 결과 공시균주 모두 304 bp에서 증폭산물이 관찰되었다. 이들 결과는 국내에서 사육되고 있는 돼지에서 S. suis 감염을 예방하는데 도움이 될 것으로 기대된다.

Keywords

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