• 대한한의학방제학회지
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• 제29권4호
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• pp.267-275
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• 2021

Objectives : Previously, we reported that compound K isolated from fermented ginseng by Aspillus oryzae has a wide biochemical and pharmacological effect, including anti-cancer activity in prostate cancer PC-3 cells. Despite these findings, its signaling pathway and gene expression pattern are not clearly understood. Methods : To confirm the gene expression study of treated with compound K in PC-3 cells, a cDNA microarray chip composed of 44K human cDNA probes was used. MTT assay, western blot analysis, propidium iodide staining, and annexin V/propidium iodide staining were analyzed. Results : We confirmed the differences of gene expression profiles. Then, we analyzed with the cell cycle arrest, cell death and cell proliferation related genes using DAVID database. Conclusions : Our finding should be useful for understanding genome-wide expression patterns of compound K-mediated cell cycle arrest toward induction of cell death and be helpful for finding future cancer therapeutic targets for prostate cancer cells.

• 한국작물학회지
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• 제63권4호
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• pp.360-377
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• 2018

The levels of 12 isoflavones were measured in soybean (Glycine max (L.) Merrill) sprouts of 68 genetic varieties from three countries (China, Japan, and Korea). The isoflavone profile differences were analyzed using data mining methods. A principal component analysis (PCA) revealed that the CSRV021 variety was separated from the others by the first two principal components. This variety appears to be most suited for functional food production due to its high isoflavone levels. Partial least squares discriminant analysis (PLS-DA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) showed that there are meaningful isoflavone compositional differences in samples that have different countries of origin. Hierarchical clustering analysis (HCA) of these phytochemicals resulted in clusters derived from closely related biochemical pathways. These results indicate the usefulness of metabolite profiling combined with chemometrics as a tool for assessing the quality of foods and identifying metabolic links in biological systems.

• Korean Chemical Engineering Research
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• 제48권2호
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• pp.147-156
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• 2010

폐가스 처리용 바이오필터의 핵심 요소 기술은 생촉매(미생물), 담체, 설계 운전 기술 및 진단 관리 기술이다. 특히, 바이오필터의 성능은 부하 조건과 바이오필터 내 미생물 군집 구조에 의해 영향을 받는다. 지금까지 바이오필터의 미생물 연구는 대부분 배양법을 기초로 하여 수행되어 왔으나, 최근에 보다 신속하고 정확하게 미생물 군집을 분석할 수 있는 방법들이 제시되고 있다. 본 논문에서는 생리적, 생화학적 및 분자생물학적 미생물 군집 분석 방법과 이를 활용한 바이오필터의 미생물 군집 특성을 조사한 연구사례를 소개하고, 미생물 군집 분석법의 바이오필터에 적용 가능성에 대해 고찰하였다. Community-level physiological profile 방법은 시료 중에 포함된 종속영양미생물의 탄소기질 이용능력을 기반으로 군집 특성을 조사하는 것이며, Phospholipid fatty acid analysis는 미생물 세포막 지방산을 분석하여 군집 특성을 조사하는 방법이다. 환경시료로부터 직접 추출한 DNA를 활용하는 분자생물학적 분석법에는 "partial community DNA analysis"와 "whole community DNA analysis"가 있다. 전자의 방법은 PCR 과정에 의해 증폭시킨 염기서열을 분석하는 것으로 ribosomal operon 유전자가 가장 많이 활용되었다. 이 방법은 다시 PCR fragment cloning 및 genetic fingerprinting으로 구분되며, genetic fingerprinting 방법으로는 denaturing gradient gel electrophoresis, terminal-restriction fragment length polymorphism, ribosomal intergenic spacer analysis 및 random amplified polymorphic DNA 방법으로 세분화된다. 추출된 전체 군집의 DNA를 분석하는 방법에는 total genomic cross-DNA hybridization, 총 추출 DNA의 열 변성/재결합 방법 및 밀도구배를 이용하여 추출한 DNA를 분획화하는 방법 등이 있다.

• 한국어병학회지
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• 제33권2호
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• pp.111-118
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• 2020

우리나라 내수면 양식 어류로부터 Edwardsiella 속 세균 7개 균주를 분리하여 이들의 생화학적 특성 및 유전학적 특성을 조사하였다. 그 결과, 기존의 어류 병원성 세균으로 알려진 E. tarda 와 E. ictaluri 뿐 아니라, 최근 새로운 종으로 보고된 E. anguillarum과 E. piscicida를 성공적으로 분리 및 동정하여 우리나라 내수면 양식 어류에서 Edwardsiella 속의 다양한 종이 분리되는 것을 확인하였다. 뱀장어류에서 분리된 4개 균주는 E. anguillarum, E. piscicida 및 E. tarda로, 메기와 동자개로부터 분리된 2개 균주는 E. ictaluri로, 버들치로부터 분리된 1개 균주는 E. piscicida로 동정되었다. 또한, 이들의 생화학적 특성의 조사 결과, 이들은 대부분 E. anguillarum, E. ictaluri, E. piscicida 및 E. tarda의 각 종에 해당하는 전형적인 생화학적 특성을 나타내었으며, 유전자를 이용한 분자계통발생학적 분석 결과와도 일치하였다. 특히, 16S rRNA 및 gyrB 유전자를 이용하여 Edwardsiella 속 종의 명확한 분류가 가능함을 확인함으로써, Edwardsiella 속 세균의 분류학적 동정을 위한 마커로써의 가능성을 제시하였다. 본 연구의 결과, 우리나라 내수면 양식 어류로부터 다양한 Edwardsiella 속 종들이 분리되는 것을 확인하였으며, 이들 종들에 대한 체계적인 모니터링 및 숙주에 따른 병원성의 차이에 관한 연구가 필요함을 제안한다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권2호
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• pp.185-191
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• 2005

An experiment was conducted to study the effect of feeding ammoniated wheat straw treated with HCl on blood biochemical profiles in growing male buffalo (Bubalus bubalis) calves. Twenty-four growing male buffalo calves (one year of age, 88.54${\pm}$3.81 kg average body weight) were divided into three groups in a completely randomized design on the basis of their body weight. Animals in all the three groups were fed on concentrate mixture. In addition, they were offered wheat straw, ammoniated wheat straw (4% urea at 50% moisture level) and HCI treated ammoniated wheat straw (4% urea at 50% moisture level and HCl added to trap 30% of the NH3 evolved) in groups I, II and III, respectively for a period of 180 days, as per Kearl (1982) for body weight gain of 500 g/d. In all diets, concentrate:roughage ratio was fixed at 50:50 and were made isonitrogenous by adjusting CP levels of conc. mixtures. Blood was collected from jugular vein of each buffalo calf at the beginning and subsequently at two months interval of experimental feeding. Due to urea-ammoniation, the CP content of wheat straw increased from 2.90 to 6.96% and addition of HCl along with urea further increased the CP content to 10.09%. In all the three groups, the mean values of plasma glucose (mg %) and serum globulin (g %), showed a decreasing trend, while the mean value of serum TP (g %), serum A:G ratio, serum urea (mg %), serum creatinine (mg %), serum ALP (KA units), SGOT (units/ml.), SGPT (units/ml), serum T$_3$ and T$_4$ (ng/ml) showed an increasing trend with the advancement of feeding period. The cumulative period mean values of serum TP (6.15 to 6.20 g %), serum albumin (3.07 to 3.18, g %), serum globulin (2.98 to 3.09, g %), serum A:G ratio (1.03 to 1.10), serum ALP (23.15 to 23.63, KA units), serum T$_3$ (1.20 to 1.23 ng/ml) and serum T$_4$ (21.33 to 21.88 ng/ml) were comparable among the groups. The cumulative period mean plasma glucose (mg %) in group III (57.28) was similar to groups I (55.31) and II (59.41), however, the cumulative period mean plasma glucose in group II was significantly (p<0.01) higher than group I. The cumulative period mean serum urea (mg %) in group III (47.34) was significantly (p<0.001) higher than group I (38.38) and II (42.24), which were statistically alike. However, the cumulative period mean serum creatinine values (mg %) in groups II (1.43) and III (1.52) were similar and were significantly (p<0.01) higher than group I (1.24). The cumulative period mean SGOT (units/ml) in groups I, II and III was 91.71, 96.04 and 96.64, respectively. Similarly the cumulative period mean SGPT (units/ml) was 19.00, 19.93 and 20.01 in groups I, II and III, respectively. The cumulative period mean values of SGOT (p<0.05) and SGPT (p<0.001) in groups II and III were similar and were significantly higher than group I. The cumulative period mean serum T$_3$ and T$_4$ values in groups I (1.21 and 21.81), II (1.23 and 21.42) and III (1.20 and 21.33) were comparable. From the present study it may be concluded that feeding of AWS treated with and without HCI to growing male buffalo calves for 180 days had no significant adverse effect on blood biochemical profile.

• Asian-Australasian Journal of Animal Sciences
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• 제26권7호
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• pp.1003-1011
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• 2013

Two experiments were conducted to determine the effects of different dietary lysine levels on the apparent nutrient digestibility, the serum amino acid (AA) concentration, and the biochemical parameters of the precaval and portal vein blood in growing pigs. In Experiment 1, 15 noncannulated pigs received diets with different lysine densities (0.65%, 0.95%, and 1.25% lysine) for 13 d. A total collection digestion test was performed, and blood samples were collected from the precaval vein at the end of the experiment. In Experiment 2, four cannulated pigs were fed the same diets of Experiment 1. The experiment used a self-control experimental design and was divided into three periods. On d 5 of each period, at 0.5 h before feeding and hourly up to 8 h after feeding, single blood samples were collected from catheters placed in the portal vein. In Experiment 1, some serum AAs (including lysine), serum urinary nitrogen (SUN), and total protein (TP) concentrations were significantly affected by the dietary lysine levels (p<0.05). Moreover, the 0.65% lysine treatment showed a significant lower apparent digestibility of gross energy, dry matter, crude protein, and phosphorus than the other treatments (p<0.05). In Experiment 2, serum lysine, histidine, phenylalanine, threonine, valine, isoleucine (p = 0.0588), triglyceride, and SUN (p = 0.0572) concentrations were significantly affected by the dietary lysine levels (p<0.05). Additionally, almost all of the determined serum AA and total AA concentrations reached their lowest values at 0.5 h before feeding and their highest values at 2 h after feeding (p<0.05). These findings indicate that the greatest absorption of AA occurred at 2 h after feeding and that the dynamic profile of serum AA is affected by the dietary lysine levels. Moreover, when the dietary lysine content was 0.95%, the growing pigs achieved a better nutrient digestibility and serum metabolites levels.

• Asian-Australasian Journal of Animal Sciences
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• 제31권9호
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• pp.1458-1463
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• 2018

Objective: This study was done to evaluate the effect of sodium stearoyl-2-lactylate (SSL) supplementation in a total mixed ration (TMR) on the lactation performance, blood parameters, and economic efficacy of mid-lactation Holstein cows. Methods: Twenty-four cows (body weight $647{\pm}11.7kg$) were randomly divided into 4 treatment groups, with six cows per group. The dietary treatments were as follows: basal diet (CON); CON+17.5 g of top dressed SSL (treatment [TRT] 0.05); CON+35 g of SSL (TRT 0.1); and CON+70 g of SSL (TRT 0.2) per 35 kg TMR. Results: The highest level of SSL supplementation (TRT 0.2) significantly improved milk yield during the second period compared to the TRT 0.05 group (5 to 8 wks; 33.28 vs 31.09 kg/d), during the third period compared to both the CON and TRT 0.05 groups (p<0.05) (9 to 13 wks; 32.59 vs 30.64 and 30.01 kg/d) and during the overall experimental period compared to both the CON and TRT 0.05 groups (p<0.05) (1 to 13 wks; 33.43 vs 32.06 and 31.40 kg/d), respectively. No negative effects on hematological or biochemical parameters were observed due to SSL supplementation. Considering both the milk fat and protein content, the total milk price was set at 1,073.60 (TRT 0.05), 1,085.60 (TRT 0.1), 1,086.10 (TRT 0.2), and 1,064.20 (CON) won/L, with consequent total milk profits of -1.7%, 5.4%, and 3.5% for the TRT 0.05, TRT 0.1, and TRT 0.2 diet, respectively, compared to those in the CON diet. Conclusion: The milk sales revenue related to SSL supplementation of the TRT 0.1 diet was increased by up to 5.4% compared to the milk sales revenue of the CON diet. Therefore, 0.1% SSL supplementation might be effective and profitable during the mid-lactation period of cows, without producing adverse effects.

• Journal of Animal Science and Technology
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• 제58권2호
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• pp.6.1-6.11
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• 2016

Background: Dietary supplementation of unsaturated fats in ruminants, if not stabilized, can instigate oxidative stress which can have negative impact on production performance and enhance the susceptibility to various diseases. The current study examined the effect of dietary 80 % canola oil and 20 % palm oil blend (CPOB) on serum fatty acids, antioxidant profile and biochemical indices in goats. Thirty Boer bucks (4-5 months old; initial BW, $20.34{\pm}0.77kg$) were randomly assigned to diets containing 0, 4 or 8 % CPOB and fed daily for a period of 90 days. Blood was sampled from the goats on 0, 30, 60 and 90 days of the trial and the serum was analyzed for fatty acids, cholesterol, glucose, total protein, antioxidants and lipid oxidation. Results: Neither diet nor sampling time influenced serum TBARS value, catalase, glutathione peroxidase and superoxide dismutase activities, LDL cholesterol, VLDL cholesterol, triglycerides, glucose and total protein. Goats fed 4 and 8 % CPOB had higher (P < 0.05) total cholesterol and HDL cholesterol than the control goats on day 30, 60 and 90. The proportion of C15:0 decreased with increasing level of CPOB on day 30 and 60. Serum C18:1n-9 increased with increasing level of CPOB in diet on day 60. The proportion of C18:3n-3 and C22:5n-3 increased (P < 0.05), while the proportion of C18:2n-6 decreased (P < 0.05) with increase in the level of CPOB on day 60 and 90. Dietary CPOB did not affect serum total carotenoid and ${\delta}$-tocopherol but did increase (P < 0.05) ${\alpha}$ and ${\gamma}$-tocopherol. Conclusion: Dietary canola oil and palm oil blend could be supplemented in diets without instigating oxidative stress in goats.

• 한국산학기술학회논문지
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• 제12권11호
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• pp.5094-5101
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• 2011

위암 완치수술을 받은 환자들의 재발수준과 재발에 미치는 영향을 파악하고자 2010년 9월부터 2011년 5월까지 한 대학병원에서 위암으로 진단받고 수술받은 환자 386명을 대상으로 조사하였다. 이들의 의무기록지와 병원암등록 자료에서 인구사회학적 특성, 생화학적 검사치, 임상병리학적 특성 등을 조사하였으며, 통계학적 검증으로 카이스퀘어 검정과 로지스틱 회귀분석을 실시하였다. 전체 조사대상자의 위암 재발률은 2.6%이었고, 성별 재발률은 남자에서 3.45%, 여자는 0.89%로 남자가 여자보다 약 4배정도 높았다. 단변량 로지스틱 회귀분석에서 재발이 발생할 위험은 림프전이가 있을수록 8.793배, 헬리코박터 파이로니균에 감염시 6.495배, 총콜레스테롤이 비정상일 때 14.333배 높았다. 다변량 로지스틱 회귀분석에서 림프전이와 헬리코박터 파이로니 감염이 재발에 중요한 요인으로 작용하였다. 결론적으로 위암의 재발에는 림프전이, 헬리코박터 파이로니 감염과 총콜레스테롤을 관리하고, CEA 및 CRP와 같은 생화학적 물질을 모니터링하여 재발을 조기에 발견하기 위한 노력이 함께 필요할 것으로 보인다.

• Journal of Periodontal and Implant Science
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• 제51권5호
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• pp.342-351
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• 2021

Purpose: The aim of this study was to compare the inflammatory and lipid profile of patients with and without peri-implantitis. Methods: A cross-sectional biochemical study was carried out in which blood samples were collected from 16 patients with peri-implantitis and from 31 subjects with healthy implants. Clinical peri-implant parameters were obtained from all subjects. Levels of tumor necrosis factor-alpha and interleukin-10 (IL-10) were measured in serum. Lipid fractions, glucose and creatinine levels, and complete blood count were also assessed. Results: After controlling for a history of periodontitis, statistically significant differences between peri-implantitis patients and controls were found for total cholesterol (estimated adjusted mean difference, 76.4 mg/dL; 95% confidence interval [CI], 39.6, 113.2 mg/dL; P<0.001), low-density lipoprotein (LDL) cholesterol (estimated adjusted mean difference, 57.7 mg/dL; 95% CI, 23.8, 91.6 mg/dL; P<0.001), white blood cells (WBC) (estimated adjusted mean difference, 2.8×10³/µL; 95% CI, 1.6, 4.0×10³/µL; P<0.001) and IL-10 (estimated adjusted mean difference, -10.4 pg/mL; 95% CI, -15.8, -5.0 pg/mL; P<0.001). The peri-implant probing pocket depth (PPD) was modestly positively correlated with total cholesterol (r=0.512; P<0.001), LDL cholesterol (r=0.463; P=0.001), and WBC (r=0.519; P<0.001). A moderate negative correlation was observed between IL-10 and PPD (r=0.609; P<0.001). Conclusions: Otherwise healthy individuals with peri-implantitis showed increased low-grade systemic inflammation and dyslipidemia.