Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Profile of Gene Expression Changes Treated with Compound K Induced Cell Cycle Arrest and Cell Death of Prostate Cancer PC-3 Cell Line

인간 전립선암 PC-3 세포에서 Compound K에 의한 세포주기 조절 및 세포사멸 유전자 발현 변화

  • Kim, Kwang-Youn (Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM)) ;
  • Park, Kwang-Il (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, Pusan National University School of Medicine)
  • 김광연 (한국한의학연구원 한의기술응용센터,) ;
  • 박광일 (경상대학교 수의과학대학) ;
  • 안순철 (부산대학교 의과대학)
  • Received : 2021.10.19
  • Accepted : 2021.11.29
  • Published : 2021.11.30
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Abstract

Objectives : Previously, we reported that compound K isolated from fermented ginseng by Aspillus oryzae has a wide biochemical and pharmacological effect, including anti-cancer activity in prostate cancer PC-3 cells. Despite these findings, its signaling pathway and gene expression pattern are not clearly understood. Methods : To confirm the gene expression study of treated with compound K in PC-3 cells, a cDNA microarray chip composed of 44K human cDNA probes was used. MTT assay, western blot analysis, propidium iodide staining, and annexin V/propidium iodide staining were analyzed. Results : We confirmed the differences of gene expression profiles. Then, we analyzed with the cell cycle arrest, cell death and cell proliferation related genes using DAVID database. Conclusions : Our finding should be useful for understanding genome-wide expression patterns of compound K-mediated cell cycle arrest toward induction of cell death and be helpful for finding future cancer therapeutic targets for prostate cancer cells.

Keywords

Acknowledgement

본 연구는 부산대학교 기본연구지원사업(2년)에 의하여 연구되었으며, 이에 감사드립니다.

References

  1. Siege RL, Miller KD, Jemal, A, Cancer statistics, 2020. CA: A Cancer Journal for Clinicians, 2020; 70(1), 7-30. https://doi.org/10.3322/caac.21590
  2. Rawla P, Epidemiology of Prostate Cancer. World Journal of Oncology, 2019; 10(2), 63-89. https://doi.org/10.14740/wjon1191
  3. Bray F, erlay, J, Soerjomataram I, Siegel RL, Torre LA, Jemal A, Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 2018; 68(6), 394-424. https://doi.org/10.3322/caac.21492
  4. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D, Global cancer statistics. CA: A Cancer Journal for Clinicians, 2011; 61(2), 69-90. https://doi.org/10.3322/caac.20107
  5. O'Neill D, Jones D, Wade M, Grey J, Nakjang S, Guo, W, et al., Development and exploitation of a novel mutant androgen receptor modelling strategy to identify new targets for advanced prostate cancer therapy. Oncotarget, 2015; 6(28), 26029-26040. https://doi.org/10.18632/oncotarget.4347
  6. Liu Y, Levine B, Autosis and autophagic cell death: the dark side of autophagy. Cell Death & Differentiation, 2015; 22(3), 367-376. https://doi.org/10.1038/cdd.2014.143
  7. Kim BH, Lee SY, Cho HJ, You SN, Kim YJ, Park YM, et al., Biotransformation of Korean Panax ginseng by Pectinex. Biological and Pharmaceutical Bulletin 2006;29(12),2472-2478. https://doi.org/10.1248/bpb.29.2472
  8. Baik IH, Kim KH, Lee KA, Antioxidant, Anti-Inflammatory and Antithrombotic Effects of Ginsenoside Compound K Enriched Extract Derived from Ginseng Sprouts. Molecules, 2021;26(13),4102. https://doi.org/10.3390/molecules26134102
  9. Zhou L, Chen L, Zeng X, Liao J, Ouyang D, Ginsenoside compound K alleviates sodium valproate-induced hepatotoxicity in rats via antioxidant effect, regulation of peroxisome pathway and iron homeostasis. Toxicology and Applied Pharmacology, 2020;386,114829. https://doi.org/10.1016/j.taap.2019.114829
  10. Kim EH, Kim W, An Insight into Ginsenoside Metabolite Compound K as a Potential Tool for Skin Disorder, Evidence-Based Complementary and Alternative Medicine, 2018;2018,8075870. https://doi.org/10.1155/2018/8075870
  11. Oh J, Kim JS, Compound K derived from ginseng: neuroprotection and cognitive improvement, Food & Function, 2016;7(11),4506-4515. https://doi.org/10.1039/C6FO01077F
  12. Yao H, Wan JY, Zeng J, Huang WH, Sava-Segal C, Li L, et al., Effects of compound K, an enteric microbiome metabolite of ginseng, in the treatment of inflammation associated colon cancer, Oncology Letters, 2018;15(6),8339-8348.
  13. Park EK, Shin YW, Lee HU, Kim SS, Lee YC, Lee BY, et al., Inhibitory effect of ginsenoside Rb1 and compound K on NO and prostaglandin E2 biosyntheses of RAW264.7 cells induced by lipopolysaccharide. Biological and Pharmaceutical Bulletin, 2005;28(4),652-6. https://doi.org/10.1248/bpb.28.652
  14. Kim MS, Lee KT, Iseli TJ, Hoy AJ, George J, Grewal T, et al., Compound K modulates fatty acid-induced lipid droplet formation and expression of proteins involved in lipid metabolism in hepatocytes. Liver International, 2013;33(10),1583-93. https://doi.org/10.1111/liv.12287
  15. Chen XJ, Liu WJ, Wen ML, Liang H, Wu SM, Zhu YZ, Ameliorative effects of Compound K and ginsenoside Rh1 on non-alcoholic fatty liver disease in rats, Scientific Reports, 2017;7,41144. https://doi.org/10.1038/srep41144
  16. Kim DY, Yuan HD, Chung IK, Chung SH, Compound K, intestinal metabolite of ginsenoside, attenuates hepatic lipid accumulation via AMPK activation in human hepatoma cells, Journal of Agricultural and Food Chemistry, 2009;57(4), 1532-1537. https://doi.org/10.1021/jf802867b
  17. Kim SH, Kim KY, Yu SN, Park SK, Kwak IS, Rhee MS, et al., Regulation of Pipernonaline on Biological Functions of Human Prostate Cancer Cells Based on Microarray Analysis, Journal of Life Science, 2012;22(11), 1552~1557. https://doi.org/10.5352/JLS.2012.22.11.1552
  18. Lammers G, Gilissen C, Nillesen ST, Uijtdewilligen PJ, Wismans RG, Veltman JA, et al., High density gene expression microarrays and gene ontology analysis for identifying processes in implanted tissue engineering constructs. Biomaterials 2010;31,8299-8312. https://doi.org/10.1016/j.biomaterials.2010.07.055
  19. Li S, Becich MJ, Gilbertson J, Microarray data mining using gene ontology. Stud. Health Technol. Inform. 2004;107,778-782.