• Title/Summary/Keyword: Biochemical decomposition

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NOGSEC: A NOnparametric method for Genome SEquence Clustering (녹섹(NOGSEC): A NOnparametric method for Genome SEquence Clustering)

  • 이영복;김판규;조환규
    • Korean Journal of Microbiology
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    • v.39 no.2
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    • pp.67-75
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    • 2003
  • One large topic in comparative genomics is to predict functional annotation by classifying protein sequences. Computational approaches for function prediction include protein structure prediction, sequence alignment and domain prediction or binding site prediction. This paper is on another computational approach searching for sets of homologous sequences from sequence similarity graph. Methods based on similarity graph do not need previous knowledges about sequences, but largely depend on the researcher's subjective threshold settings. In this paper, we propose a genome sequence clustering method of iterative testing and graph decomposition, and a simple method to calculate a strict threshold having biochemical meaning. Proposed method was applied to known bacterial genome sequences and the result was shown with the BAG algorithm's. Result clusters are lacking some completeness, but the confidence level is very high and the method does not need user-defined thresholds.

Estimation of Biochemical Degradation in Landfill Waste (사후관리단계 매립지의 생화학적 안정성 평가 연구)

  • Yoo, Kee-Young;Yi, So-Ra
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.1
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    • pp.109-118
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    • 2001
  • In the stage of aftercare in waste landfill management, it is very difficult to estimate the decomposition of landfill waste by excavation which damages the low permeability layer. This study developed the method to analyze the amount of landfill waste degraded bio-chemically as the types of leachate and gas, and applied the method to Nanjido landfill(NL). Application result showed that 70% of high biodegradable waste in NL was transformed to gas and leachate by 2000. Also this study suggested that the transformed portions of waste name for "Decomposition Index" at that time and the proposed method must be modified according to the biological condition of waste degradation.

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Anaerobic Digestion of Fish Offal(II) : Evaluation of Biodegradability Using Biochemical Methane Potential (생선 폐기물의 혐기성 소화 처리(II) : Biochemical Methane Potential을 이용한 생분해도 평가)

  • Jeong Byung-Gon
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.9 no.3
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    • pp.154-159
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    • 2006
  • It is essential to understand the decomposition characteristics for developing the optimum anaerobic digestion system of organic wastes. In this study, BMP (Biochemical Methane Potential) test using serum bottle was conducted to evaluate the anaerobic degradability of fish offal. 3 different groups of fish offal including waste from mackerel and hairtail handling except viscera and fish viscera were chosen for the substrates. Grinded fish offal was transferred anaerobically to serum bottle in amounts of 50 ml, 100 ml and 150 ml, respectively. BMP test was carried out in triplicate. Cumulative methane production and methane production rate depending on incubation time were evaluated. These results varied depending on substrate characteristics. The average values of ultimate methane yield ranged between $420ml{\cdot}CH_4/g{\cdot}VS$ and $490ml{\cdot}CH_4/g{\cdot}VS$, and the methane production and degradation rate of viscera were higher than those of other parts of fish offal. According to the analysis of elemental composition, average C/N ratio of fish offal used in this study was 5.2. Theoretical ultimate methane yield calculated from elemental composition was $522ml{\cdot}CH_4/g{\cdot}VS$. Biodegradability was calculated as 0.847.

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Formulating Analytical Solution of Network ODE Systems Based on Input Excitations

  • Bagchi, Susmit
    • Journal of Information Processing Systems
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    • v.14 no.2
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    • pp.455-468
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    • 2018
  • The concepts of graph theory are applied to model and analyze dynamics of computer networks, biochemical networks and, semantics of social networks. The analysis of dynamics of complex networks is important in order to determine the stability and performance of networked systems. The analysis of non-stationary and nonlinear complex networks requires the applications of ordinary differential equations (ODE). However, the process of resolving input excitation to the dynamic non-stationary networks is difficult without involving external functions. This paper proposes an analytical formulation for generating solutions of nonlinear network ODE systems with functional decomposition. Furthermore, the input excitations are analytically resolved in linearized dynamic networks. The stability condition of dynamic networks is determined. The proposed analytical framework is generalized in nature and does not require any domain or range constraints.

Effects of Municipal LFG Characteristics on Settlement (도시폐기물 매립지의 LFG 특성이 침하에 미치는 영향)

  • Ahn, Tae-Bong;Jin, Han-Kyu;Park, Dae-Hyo
    • Proceedings of the Korean Geotechical Society Conference
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    • 2004.03b
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    • pp.461-468
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    • 2004
  • The amount of $CO_2$ and $CH_4$ that were produced during biodegradation with were measured as function of time. Also, the settlements of landfill lysimeters were measured at the same time, and the relation with LFF(landfill gas) production was investigated. The effects of leachate recycle on the acceleration of settlement were studied by comparing two lysimeters. The gas production rate constant, $^{\kappa}_{LFG}$ is obtained to predict future gas production and settlement. These will be key role factors to predict gas production patterns and residual long-term settlement.

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Decomposition of Biological Macromolecules by Plasma Generated with Helium and Oxygen

  • Kim Seong-Mi;Kim Jong-Il
    • Journal of Microbiology
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    • v.44 no.4
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    • pp.466-471
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    • 2006
  • In this study, we attempted to characterize the biomolecular effects of an atmospheric-pressure cold plasma (APCP) system which utilizes helium/oxygen $(He/O_2)$. APCP using $He/O_2$ generates a low level of UV while generating reactive oxygen radicals which probably serve as the primary factor in sterilization; these reactive oxygen radicals have the advantage of being capable to access the interiors of the structures of microbial cells. The damaging effects of plasma exposure on polypeptides, DNA, and enzyme proteins in the cell were assessed using biochemical methods.

Biochemical Methane Potential and Biodegradability of Animal Manure and Cultivated Forage Crops at the Reclaimed Tideland (가축분뇨와 간척지 사료작물의 메탄발생량과 생분해도)

  • Heo, Nam-Hyo;Lee, Seung-Heon;Kim, Byeong-Ki
    • New & Renewable Energy
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    • v.4 no.4
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    • pp.56-64
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    • 2008
  • Anaerobic biodegradability (AB), which can be determined with the ultimate methane yield by the decomposition of organic materials, is one of the important parameters for the design and the operation of anaerobic digestion plant. In this study, Biochemical methane potential (BMP) test has been carried out to evaluate the methane yields of animal manures such as pig and cattle slurries, and different forage crops cultivated at the reclaimed tideland such as maize, sorghum, barley, rye, Italian ryegrass (IRG), rape, rush, and waste sludge produced from slaughterhouse wastewater treatment plant (SSWTP). In the ultimate methane yield and biodegradability of animal manure, those of pig slurry were 345 $mlCH_4/gVS_{fed}$ and 44.7% higher than 247 $mlCH_4/gVS_{fed}$ and 46.4% of cattle slurry (Cat. 2). The ultimate methane yield and biodegradability of spike-crop rye (Rye 1) were 442.36 $mlCH_4/gVS_{fed}$ and 86.5% the highest among different forage crops, those of the other forage crops ranged from 306.6 to 379 $mlCH_4/gVS_{fed}$ of methane yield with the AB having the range of about 60 to 77%. Therefore the forage crops could be used as a good substrate to increase the methane production and to improve the biodegradability in anaerobic co-digestion together with animal manure.

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The Effects of the Content of Isobornyl Methacrylate in Acrylate Copolymers on Physical Properties (아크릴계 공중합체에서 이소보닐 메타크릴레이트의 함량에 의한 물리적 특성 변화)

  • Kim, Ki Sang;Shim, Sang-Yeon
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.4
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    • pp.771-776
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    • 2016
  • The acrylate copolymer having good thermal stability, coating and adhesion properties was designed and prepared. We prepared copolymers in >95% high yield using methyl methacrylate, isobornyl methacrylate and 2-hydroxyethyl methacrylate monomers by the bulk and emulsion polymerization techniques. The $^1H$-NMR spectrum was used to identify chemical structure and glass transition temperatures increased from $123^{\circ}C$ to $140^{\circ}C$ confirmed by DSC, DMA and TGA analysis. In addition, as the content of IBMA increased, storage modulus and thermal decomposition temperature increased. As the content of IBMA increased from 10% to 30% in the composition for the entire monomer, tensile strength increased from 22 to 30 MPa in both polymers prepared by bulk and emulsion techniques. The contact angle increased from 70 to up to 88 degrees due to hydrophobic property of IBMA.

Development of Nanoenzymes for the Production of Glucose from Seaweed and Various Polysaccharide (해조류 및 다당류로부터 포도당 생산을 위한 나노효소 개발 및 특성)

  • Jin, Lie-Hua;Lee, Jung-Heon
    • KSBB Journal
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    • v.25 no.5
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    • pp.453-458
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    • 2010
  • The magnetically separable polyaniline nanofiber enzymes were developed for the recycle of enzyme and enhanced enzyme stability. The stability of enzyme was maintained over 90% for 8 days under room temperature and vigorous shaking conditions (200 rpm). The residual activity of immobilized enzyme was over 60% after 8 days incubation at $55^{\circ}C$. Glucose was produced from various polysaccharides, agarose, curdlan, cellulose, and sea weed, using magnetically separable immobilized enzyme. Glucose production rate with curdlan was 1.2 g/(l h) and showed high decomposition rate due to high mass transfer. After 10 times recycle, the residual activity of immobilized enzyme was over 75%. 1 g/L of glucose was produced with 5 mg of immobilized enzymes.

Characterization of a Ligninase Producing Strain, Serratia marcescens HY-5 isolated from Sympetrum dopressiusculum (고추좀잠자리 (Sympetrum depressiusculum)로부터 분리한 리그닌 분해균주, Serratia marcescens HY-5의 특성)

  • Kim, Ki-Duck;Park, Doo-Sang;Shin, Dong-Ha;Han, Bo-Na;Oh, Hyun-Woo;Youn, Young-Nam;Park, Ho-Yong
    • Korean journal of applied entomology
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    • v.45 no.3 s.144
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    • pp.301-307
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    • 2006
  • A ligniolytic bacterial strain was isolated from the digestive tract of a red dragonfly, Sympetrum dopressiusculum. It was identified as a Serratia marcescens HY-5 by 16S rDNA sequence analysis and physiological and biochemical analysis. The isolated strain showed proportional increase of ligninolytic activity to the cell growth in the culture media which include lignin compounds. It showed about 25-45% decomposition of lignin compound by 48 hr incubation especially, showed effective decomposition of monomer lignin compounds, vanillin and guaiacol, and a dimer, dealkaline lignin. PCR amplification of 16S rDNA followed by denaturing gradient gel electrophoresis analysis showed high density of S. marcescens HY-5 in the gut of the S. depressiusculum at both gut samples which collected at different site.