• KSBB Journal
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• 제28권2호
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• pp.131-136
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• 2013

The hydrolysates prepared with various enzyme digestion of Capsosiphon fulvescens were used to measure the inhibitory effects against angiotensin I converting enzyme (ACE). The commercially available enzymes such as Celluclast, Viscozyme, Lysing enzyme, Flavourzyme, Alcalase and Pectinex were used to digest C. fulvescens and produce hydrolysates. The maximum ACE inhibitory activity was observed using Alcalase hydrolysis (72.9%). The optimal conditions of Alcalase extraction were pH 8.0 and extraction time for 12 hr. The hydrolysates were fractionated using preparative-LC and anion-exchange chromatography on DEAE-cellulose and the fraction B and B-2 were isolated. The ACE inhibitory activity of fraction B-2 by anion-exchange chromatography was 82.6%. The molecular weight of fraction B-2 estimated using size exclusion chromatography was about 1 kDa. The monosaccharide composition of the fraction B-2 was determined to be mannose (1.1%), glucuronic acid (1.3%), galactose (1.3%) and glucose (96.3%).

• 미생물학회지
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• 제28권3호
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• pp.249-257
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• 1990

In the course of operating the accommodative and detoxifying mechanism against cadmium, its physio-biochemical changes were observed in Klebsiella aerogenes ATCC 10031. Changes of enzyme activity concerned phosphate metabolism, changes of phospholipid composition and in view of energy metabolism the changes of the nucleotide pool were examined. Activities of both alkaline and acid phosphatase were derepressed 4-10 folds under cadmium added cultures. Moreover, production of phospholipid such as lysophosphatidyl choline (LPC), phosphatidyl serine (PS) and phosphatidyl ethanolamone (PE) was increased and uridylate nucleotide pool was increased under Cd-surplus culture. These results i.e. overproduction of phosphatase catalyzing phosphate residue, increase of the production of PE and PS which have a close affinity with cadmium, and indrease of uridylate nucleotide pool used as a carrier of polysaccharide synthesis like bacterial capsule exhibited cellular responses for active defence against Cd-pressure. It was postulated that these phenomena should play another assistant roles in Cd-detoxifing mechanism.

• Journal of Industrial and Engineering Chemistry
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• 제67권
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• pp.417-428
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• 2018

In this study, we fabricated hierarchically self-assembled thin films composed of graphene oxide (GO) sheets and gold nanoparticles (Au NPs) using the Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) techniques and investigated their gas-sensing performance. First, a thermally oxidized silicon wafer ($Si/SiO_2$) was hydrophobized by depositing the LB films of cadmium arachidate. Thin films of ligand-capped Au NPs and GO sheets of the appropriate size were then sequentially transferred onto the hydrophobic silicon wafer using the LB and the LS techniques, respectively. Several different films were prepared by varying the ligand type, film composition, and surface pressure of the spread monolayer at the air/water interface. Their structures were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM), and their gas-sensing performance for $NH_3$ and $CO_2$ was assessed. The thin films of dodecanethiol-capped Au NPs and medium-sized GO sheets had a better hierarchical structure with higher uniformity and exhibited better gas-sensing performance.

• Mass Spectrometry Letters
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• 제5권4호
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• pp.115-119
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• 2014

The Brazil nut (Bertholletia excelsa - Lecythidaceae) is considered a product with high economic value, being a food widely appreciated for its nutritional qualities. Although previous studies have reported the biochemical composition of Brazil nut oil, the knowledge regarding the phospholipid composition exhibits a disagreement: the composition of fatty acids present in the structures of phospholipids is reported as being different from the composition of the free fatty acids present in the oil. In this work, solid phase extraction (SPE) was employed to provide a fast extraction of the phospholipids from Brazil nuts, in order to compare the phospholipid profile of the in nature nuts and their fatty acids precursor present in the oil. The major phospholipids were characterized by mass spectrometry approach. Their fragmentation pattern through direct infusion electrospray ionization ion-trap tandem mass spectrometry ($ESI-IT-MS^2$) proved to be useful to unequivocal characterization of these substances. High resolution (HR) experiments through ESI using a quadruple time of flight mass spectrometry (QTOF) system were performed to reinforce the identifications.

• Animal Bioscience
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• 제37권5호
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• pp.883-895
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• 2024

Objective: This study aimed to investigate the effects of dietary supplementation of a fatty acid-balanced oil, instead of soybean oil, on reproductive performance, nutrient digestibility, blood indexes, milk composition in lactating sows, and fecal microbial composition in piglets. Methods: Twenty-four sows (Landrace×Yorkshire, mean parity 4.96) were randomly allotted to two treatments with twelve pens per treatment and one sow per pen based on their backfat thickness and parity. The experiment began on day 107 of gestation and continued until weaning on day 21 of lactation, lasting for 28 days. The control group (CG) was fed a basal diet supplemented with 2% soybean oil and the experimental group (EG) was fed the basal diet supplemented with 2% fatty acid-balanced oil. Results: The fatty acid-balanced oil supplementation increased (p<0.05) the apparent total tract digestibility of dry matter, crude protein, and gross energy in sows. The lower (p<0.05) serum high-density lipoprotein cholesterol and albumin levels of sows were observed in the EG on day 21 of lactation. Dietary supplementation with the fatty acid-balanced oil decreased the fat content, increased the immunoglobulin G level, and changed (p<0.05) some fatty acid content in milk. Moreover, the fatty acid-balanced oil supplementation changed (p<0.05) the fecal microbial composition of piglets, where the average relative abundance of Spirochaetota was decreased (p<0.05) by 0.55% at the phylum level, and the average relative abundance of some potentially pathogenic fecal microorganism was decreased (p<0.05) at the species level. Conclusion: The fatty acid-balanced oil improved nutrient digestibility, changed the serum biochemical indices and milk composition of sows, and ameliorated the fecal microbial composition of piglets.

• 한국잠사곤충학회지
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• 제31권2호
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• pp.82-90
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• 1989

분리.정제한 전잠 vitellin의 분자량추정, 당 및 지질성분의 존재유무, 전기현미경적인 형태 및 크기, 아미노산 조성, 내열성 및 여러 가지 특성을 조사하였다. 1. 분리.정제된 천잠의 vitellin은 전기영동상의 이동도가 다른 large 및 small subunit로 되어 있다. 2. 천잠 vitellin의 분자량은 약 450,000d로 추정되었고, large subunit와 small subunit는 각각 174,000d와 44,000d로 추정되었다. 3. Coomassie brilliant blue, sudan black B 및 PAS 염색에 의해서 천잠의 vitellin은 glycolipoprotein으로 동정되었으며, 2개의 subunit도 당과 지질성분을 가지고 있었다. 4. 천잠 vitellin의 지질성분 중에는 가잠이나 Eri-참에 비하여 매우 다양한 종류들이 검출되며 특히 포화지방이라고 추정되는 물질도 있었다. 5. 천잠 vitellin의 단백질과 당성분은 7$0^{\circ}C$에 60분까지는 안전성을 보였으나, 같은 조건에서 지질성분은 검출되지 않았다. 6. 천잠 vitellin의 아미노산 성분 중 다른 곤충에서는 함량이 적은 glycine이 현격히 많았고 기타 성분의 함량은 가잠이나 Eri-잠과 같은 경향이었다. 7. 천잠의 vitellin은 직경이 14$\pm$0.8nm의 균일한 구형이었다.

• 환경생물
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• 제18권4호
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• pp.395-401
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• 2000

질산염과 인산염의 농도가 다른 배지에서 Gymnodinium sanguineum과 Sketetonema costotum의 성장과 세포구성성분을 비교 분석하였다. 질산염은 0, 0.3, 0.6, 0.9, 1.2 mM, 인산염은 0, 15, 30, 45, 60$\mu$M을 각각 첨가한 배지에서 배양하였을 때, 저농도의 질산염 및 인산염 배지에서 G. sanguineum의 성장은 억제되었으나, S. costatum은 성장에 영향을 받지 않은 것으로 나타났다. 저농도의 질산염과 인산염 배지에서 G. sanguineum의 세포내 단백질 및 탄수화물의 양은 감소하였으나, S. costutum은 탄수화물의 양이 조금 증가하는 경향을 보였다. 세포내 지질은 G. sunguineum의 경우 중성지질과 인지질은 변화가 거의 없는 반면, 당지질은 저농도의 질산염 및 인산염에서 크게 감소하였다. 그러나 S. costatum 세포내의 세 종류의 지질의 양은 변화를 보이지 않았다. 이와 같은 결과는 저농도의 질산염 및 인산염 배지에서 와편모조류인 G. sanguineum보다 규조류인 S. costatum이 더 잘 적응할 수 있다는 것을 보여준다.

• 한국수산과학회지
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• 제34권4호
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• pp.340-347
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• 2001

한반도 남해안의 고성만과 강진만에 대한 입자유기물질 양과 현탁물식자를 위한 먹이로서의 질의 월별변동을 밝히기 위하여 부유입자물질의 원소 및 생화학 조성에 대한 현장조사를 1999년7월부터 2000년 8월까지 실시하였다. 두 해역의 총 부유입자물질은 해역간에 유사한 농도 분포로 뚜렷한 월별 변동 양식을 나타내지 않았다. 그러나 이들 생물군의 잠재 먹이원인 입자유기물질 조성성분들은 두 해역 모두 여름철에 뚜렷이 높은 농도를 보이는 계절 변동성을 나타내었다. 한편, 입자유기물질의 원소나 생화학 조성 성분의 높은 농도가 나타나는 시기는 chlorophyll a와 잘 일치하여 식물플랑크톤 생산이 입자유기물질의 중요한 조절자라는 것을 가리킨다. 그러나, chlorophyll a와 입자유기물질 조성성분(입자 단백질, 탄수화물 및 지질)들 사이에 유의한 상관관계는 나타나지 않았고, 이들 생화학 성분들 간에도 높은 상관성은 발견되지 않았다 이와 같은 결과들은 식물플랑크톤 생물량이 낮은 시기에 연구 해역들의 입자유기물질에 대한 또 다른 과정들에 의한 기여가 있다는 것을 시사하였는데, 가을에서 봄철사이에 높은 C:Chl a, C:N 비 및 입자 탄수화물의 높은 농도 등은 입자유기물질의 생화학성분 농도가 표층 퇴적물 재부유나 양식생물의 배설과 같은 과정들에 의해 영향을 받고 있다는 것을 나타낸다. 본 조사해역에서 입자유기물질 중 생화학 성분들의 합으로 나타낸 먹이물질은 그것들의 에너지 값과 함께 가을부터 봄철사이에는 다소 높은 농도가 관찰되었지만, 가장 높은 농도는 여름철에 나타났다. 총 부 유입자물질 중 먹이물질이 차지하는 비율로서 표시한 먹이지수는 대체로 $6\%$를 넘지 않는 수준으로 입자유기물질의 영양상태에 있어서 다소 낮은 질을 나타내는 것으로 평가되었다. 본 연구 결과는 식물플랑크톤 변동과 함께 입자유기물질의 먹이물질과 먹이지수 변동을 동시에 측정함으로서 현탁물 섭식자 생물군의 성장과 비만 및 생리생태를 이해하기 위한 자연의 먹이환경으로서 입자유기물질 농도나 질을 더욱 효과적으로 나타낼 수 있다는 것을 보인다.

• 대한지역사회영양학회지
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• 제2권5호
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• pp.701-710
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• 1997

this study was undertaken to compare the riboflavin status of rural women with different physical activity intensity and to determine factors influencing biochemical fiboflavin status. The study was carried out over three different farming seasons : planting (June), harvest(October) and interim(February) in two rural regions of Korea. One was a traditional farming region, the other a commercial farming region with heavier work intensity. Twenty women in the traditional region and eighteen women in the commercial region were involved. The intensity of physical activity was determined by a daily activity record. Body composition was assessed by bioelectrical impedence. Dietary riboflavin intake was measured by the food frequency method. Riboflavin biochemical status was assessed by erythrocyte glutathione reductase activity coefficient (EGR AC) and ruinary riboflavin excretion. The results from the EGR AC and urinary riboflavin excretion during the period showed the overall riboflavin status of the commercial farming women was significantly worse than that of the traditional farming women(EGR AC p<0.0001, urinary riboflavin excretion p<0.05). The traditional farming group had about 40% with risk of riboflavin deficiency, whereas the commercial farming group had about 70%. Overall mean nutrient intake was not significantly different between the two groups, however, overall mean percent lean body mass representing long term physical activity was significantly higher in the commercial farming group ( <0.005). It appears that the biochemical riboflavin status of traditional farming women was significantly influenced by riboflavin intake and crude nitrogen balance while the biochemical riboflavin status of the commercial farming women was significantly influenced by riboflavin intake and percent of lean body mass over the three seasons. (Korean J Community Nutrition 2(5) : 701∼710, 1997)

• 멤브레인
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• 제34권2호
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• pp.146-153
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• 2024

본 실험에서는 Ti를 기반으로 한 평판 수소 분리막을 설계하여 제조하였다. 새로운 조성의 Ti를 베이스로 한 수소 분리막을 찾기 위하여 여러 합금들의 물리화학적 특성과 수소투과도 사이의 상관관계에 대해 조사하였다. 이를 바탕으로 신조성의 합금막 2종(Ti_14.2Zr_66.4Ni_12.6Cu_6.8 (70 ㎛), Ti_17.3Zr_62.7Ni₂₀ (80 ㎛))을 설계 및 제조하였다. 제조된 평판 수소 분리막은 300~500℃, 1~4 bar의 조건에서 혼합 가스(H₂, N₂), sweep 가스(Ar)를 이용하여 수소 투과 실험을 진행하였다. Ti_14.2Zr_66.4Ni_12.6Cu_6.8 합금막은 500℃, 4bar에서 최대 16.35 mL/cm² min의 flux를 가지며, Ti_17.3Zr_62.7Ni₂₀ 합금막은 450℃, 4 bar에서 최대 10.28 mL/cm² min의 flux를 가진다.