• Archives of Pharmacal Research
• /
• 제18권4호
• /
• pp.224-230
• /
• 1995

Recently, many attempts have been made to use hydrogels of various polymers as delivery systems of various drugs and bioactive materials to prolong and control their phamacological activities. In this study, we have evaluated the physico-chemical properties of methacrylic acid-methyacrylic acid methyl ester copolymer 9Eudispert mv)m a acrylic resin hydorgel, and its application to transdermal delivery system. In the dissolution tests, the release rate of salicylic acid (SA) and sodium salicylate (SOd. SA) were faster than lidocain (LD) and lidocain-HCl(LD-HCl). As the concentration of Eudispert mv polymer increased, the extensibility of Eudispert mu hydrogel decreased, whereas the swelling ratio increased. The more NaOH and polymer concentration increased, the more osmotic pressure linearly increased. The skin permeation of Sod. SA, an acidic model drug, was remarkably enhanced by Eudispert mv hydrogel. All fatty acids, except for Sod. glycolate, dramatically increased the skin permeation flux in Eudispert mu hydrogel containing LD-Hcl, a basic model drug. Consequently, it is suggested that Eudispert mv hydrogel may be used as potential transdermal delivery vehicle.

• 한국식품저장유통학회지
• /
• 제30권4호
• /
• pp.589-601
• /
• 2023

Malaysian honey is rich in nutrients and bioactive compounds, which can be a healthy alternative to refined sugar in food production. However, liquid honey's viscous and sticky nature makes it unpreferable in industrial handling. This study, an atomization system coupled with vacuum drying to produce honey powders to overcome the problem. Three types of Malaysian honey, namely Acacia, Gelam, and Tualang, were encapsulated in Ca-alginate gel beads using the atomization system. The density viscosity, and surface tension of the honey-alginate solutions were measured, and the concentration of honey and alginate influenced the physical properties of the solutions. Honey-encapsulated gel beads in the size range of 2.16-2.92 mm were produced using the atomization system with the air-liquid mass flow rate ratios of 0.22-0.31, Weber number (We) of 112-545, and Ohnersorges number (Oh) of 0.35-10.46. Gel bead diameter can be predicted using a simple mathematical model. After vacuum drying, the honey gel powder produced was in the size range of 1.50-1.79 mm. Results showed that honey gel powders with good encapsulation efficiency and high honey loading could be produced using the atomization system and vacuum drying.

• Corrosion Science and Technology
• /
• 제23권1호
• /
• pp.72-81
• /
• 2024

The Ti-6Al-4V alloy is widely used as an implant material due to its higher fatigue strength and strengthto-weight ratio compared to pure titanium, excellent corrosion resistance, and bone-like properties that promote osseointegration. For rapid osseointegration, the adhesion between the titanium surface and cellular biomolecules is crucial because adhesion, morphology, function, and proliferation are influenced by surface characteristics. Polymeric peptides and similar coating technologies have limited effectiveness, prompting a demand for alternative materials. There is growing interest in 2D nanomaterials, such as MoS₂, for good corrosion resistance and antibacterial, and bioactive properties. However, to coat MoS₂ thin films onto titanium, typically a low-temperature hydrothermal synthesis method is required, resulting in the synthesis of films with a toxic 1T@2H crystalline structure. In this study, through high-temperature annealing, we transformed them into a non-toxic 2H structure. The implant coating technique proposed in this study has good corrosion resistance and biocompatibility, and antibacterial properties.

• 생명과학회지
• /
• 제22권2호
• /
• pp.226-231
• /
• 2012

Cordycepin 고함유 번데기 동충하초(Cordyceps militaris JLM0636, $CM{\alpha}$)와 일반 번데기 동충하초(Cordyceps militaris, CM)의 생리활성 물질 및 영양성분 분석과 혈전 용해 활성을 비교 검토하였다. Total polyphenol compound, flavonoids 및 glutathione 함량은 CM 보다 $CM{\alpha}$에서 높은 함량을 보였다. CM 및 $CM{\alpha}$의 주요 미네랄 구성 성분으로는 K, Ca, Mg, Na이고, 주요 지방산 조성은 linolenic acid, linoleic acid, oleic acid, palmitic acid으로 나타났다. 혈전 용해 활성을 비교 검토한 결과 $CM{\alpha}$이 CM보다 높은 활성을 보였다. 이상의 실험 결과 $CM{\alpha}$의 생리활성물질과 혈전 용해 활성을 이용한 제품 개발에 필요한 기초 자료를 제공하고 유용하게 사용될 수 있을 것으로 사료되어 진다.

• 대한치과보철학회지
• /
• 제34권1호
• /
• pp.169-186
• /
• 1996

The purpose of this study was to investigate the mechanical properities and biocompatibility with crystallization temperature and time of a bioactive glass-ceramic system $41.4wt%SiO_{2}-35.0wt%CaO-3.0wt%MgO-12.0wt%P_{2}O_{5}-8.6wt%Al_{2}O_{3}$ with same molar percent of $Al_{2}O_{3}\;and\;P_{2}O_{5}$. The crystallization behaviors were investigated with DTA, XRD and SEM. Fracture toughness with the change of crystallization temperature and time was measured by indentation fracture method. Also, biocompatibility was evaluated by culture of mouse fibroblast cell line L929. The results obtained were as follows ; 1. The major crystalline phases were apatite and anorthite, and relative intensity of anorthite phase was increased at $1004^{\circ}C$. 2. The hardness and fracture toughness were gradually increased with the increase in ceraming temperature to $1004^{\circ}C$. 3. When the glass ceramic was heat-treated for 4 hours at ceraming temperature of $1004^{\circ}C$, hardness and fracture toughness showed the maximum values $578.84k/mm^2\;and\;2.07MPa\;m^{1/2}$, respectively. 4. The growth rate and cytotoxic of L929 fibroblast cells for bioactive glass ceramic were better than those of stainless steel and titanium.

• Journal of Microbiology and Biotechnology
• /
• 제26권10호
• /
• pp.1675-1686
• /
• 2016

We characterized the probiotic properties of Lactobacillus helveticus strains KII13 and KHI1 isolated from fermented cow milk by in vitro and in vivo studies. The strains exhibited tolerance to simulated orogastrointestinal condition, adherence to Caco-2 cells, and antimicrobial activity. Both L. helveticus strains produced bioactive tripeptides, isoleucylprolyl-proline and valyl-prolyl-proline, during fermentation of milk. KII13 showed higher in vitro cholesterol-lowering activity (47%) compared with KHI1 (28%) and L. helveticus ATCC 15009 (22%), and hence, it was selected for in vivo study of cholesterol-lowering activity in atherogenic diet-fed hypercholesterolemic mice. For the study, mice were divided into four groups (viz., normal diet control group, atherogenic diet control group (HCD), KII13-atherogenic diet group (HCD-KII13), and Lactobacillus acidophilus ATCC 43121-atherogenic diet group (HCD-L.ac) as positive control). The serum total cholesterol level was significantly decreased by 8.6% and 7.78% in the HCD-KII13 and HCD-L.ac groups (p < 0.05), respectively, compared with the HCD group. Low-density lipoprotein cholesterol levels in both HCD-KII13 and HCD-L.ac groups were decreased by 13% and 11%, respectively, compared with the HCD group (both, p < 0.05). Analysis of cholesterol metabolism-related gene expression in mice liver showed increased expression of LDLR and SREBF2 genes in mice fed with KII13. By comparing all the results, we conclude that L. helveticus KII13 could be used as a potential probiotic strain to produce antihypertensive peptides and reduce serum cholesterol.

• BMB Reports
• /
• 제36권4호
• /
• pp.337-343
• /
• 2003

Rhus verniciflua Stokes (RVS) has been used as a traditional herbal medicine. Several earlier studies indicated that an ethanol extract of RVS has both anti-oxidant and anti-tumor properties, although the mechanism for the activity remains to be elucidated. In this report, we prepared a highly purified ethanol extract from RVS, named REEE-1 ($\underline{R}$hus $\underline{e}$thanol $\underline{e}$luted $\underline{e}$xtract-1), and investigated the mechanism involved in its growth-inhibitory effect on the human B and T lymphoma cell lines, BJAB and Jurkat, respectively. Results from tritium uptake proliferation assays showed that the proliferative capacities of both BJAB and Jurkat cells were strongly suppressed in the presence of REEE-1. This was further confirmed through trypan blue exclusion experiments that revealed a dose-dependent decrease in viable cell numbers after REEE-1 treatment. REEE-1-mediated suppression of cell growth was verified to be apoptotic, based on the increase in DNA fragmentation, low fluorescence intensity in nuclei after propidium iodide staining, and the appearance of DNA laddering. In particular, REEE-1 exerted its anti-oxidant activity through the inhibition of hydroxyl radical-mediated degradation by iron ion chelation rather than direct scavenging of hydroxyl radicals. Furthermore, REEE-1 was revealed to be a potential scavenger of superoxide anions. Collectively, our findings suggest that REEE-1 is a natural anti-oxidant that could be used as a cancer chemo-preventive and therapeutic agent.

• 한국식품위생안전성학회지
• /
• 제33권3호
• /
• pp.151-156
• /
• 2018

해양생물에는 육상생물자원에서는 존재하지 않는 다양한 화합물이 많이 존재하는데 이들 화합물은 새로운 치료제 및 대체 치료법을 개발하는데 유용하게 이용될 수 있다. 현재 해조류의 다양한 생리활성에 대한 연구가 진행되고 있으며 최근에는 여러 병원성 및 인체 감염균에 대한 항균효과를 나타내어 신약개발의 보고로 다양한 연구가 진행이 되고있다. 즉, 해조류는 천연물신약 또는 새로운 치료제 개발에 중요한 생물자원이다.

• 생명과학회지
• /
• 제21권7호
• /
• pp.1016-1024
• /
• 2011

영지버섯, 여주, 쓴메밀 및 이들 혼합 시료의 70% 메탄올 추출물을 이용하여 생리활성물질(폴리페놀 화합물 및 플라보노이드) 농도와 항산화(DPPH radical 소거 활성, 생체막 지질과산화, Fe/Cu 환원력), ${\alpha}$-glucosidase 저해, tyrosinase 저해 및 항혈전 활성에 대하여 검토하였다. 추출 수율과 폴리페놀 화합물 및 플라보노이드의 함량은 혼합 추출물에서 가장 높았다. 또한 혼합 추출물에서 ${\alpha}$-glucosidase 저해, tyrosinase 저해, 항산화, 환원력 및 항혈전 활성은 혼합 추출물에서 가장 높았으며, 이러한 효과는 처리 농도 의존적 이였다. 이상의 실험 결과에서 영지버섯, 여주, 쓴메밀의 혼합 메탄올 추출물은 폴리페놀 화합물과 플라보노이드를 많이 함유함으로서 높은 ${\alpha}$-glucosidase 저해 및 항산화 활성과 관련성이 높아 보여 항당뇨 건강 기능성식품의 소재 개발에 활용될 가능성이 높아 보인다.

• 한국축산식품학회지
• /
• 제39권6호
• /
• pp.966-979
• /
• 2019

Muscle-based by-products are often undervalued although commonly reported having a high amount of natural bioactive peptides. In this study, elastin was isolated from the protein of broiler hen skin while its hydrolysate was prepared using Elastase. Assessment of antioxidative properties of elastin-based hydrolysate (EBH) was based on three different assays; 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical, 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical and metal chelating ability. The EBH was purified further using ultrafiltration, gel filtration and Reverse- Phase High-Performance Liquid Chromatography (RP-HPLC). The IC₅₀ of ABTS radical activities for EBH were decreased as EBH further purified using ultrafiltration (EBH III; 0.66 mg/mL)>gel filtration (EB-II; 0.42 mg/mL)>RP-HPLC (EB-II4; 0.12 mg/mL). The sequential identification of the peptide was done by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/ TOF-MS) of the potent fractions obtained from RP-HPLC (EB-II4). The presence of hydrophobic amino acids (Val and Pro) in the peptide sequences could potentially contribute to the high antioxidant activity of EBH. The sequences GAHTGPRKPFKPR, GMPGFDVR and ADASVLPK were identified as antioxidant peptides. In conclusion, the antioxidative potential from poultry skin specifically from elastin is evident and can be explored to be used in many applications such as health and pharmaceutical purposes.