• Food Science and Preservation
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• v.24 no.7
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• pp.885-890
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• 2017

For the construction of the microbial monitoring method, anti-Salmonella polyclonal antibodies (pAbs) were produced from a rabbit and purified by saturated ammonium sulfate precipitation and protein A affinity column. The reactivity of anti-Salmonella pAbs was compared to that of commercial ones by using an indirect ELISA. The specificity of anti-Salmonella pAbs was investigated using 20 Salmonella serotypes and 20 non-Salmonella strains. A capturing ability of anti-Salmonella pAbs was investigated by exposing antibody-immobilized gold biosensor to different concentration of Salmonella mixture. Anti-Salmonella pAbs were successfully produced and purified with an antibody concentration of 2.0 mg/mL The reactivity of purified anti-Salmonella pAbs was greater than that of commercial one at all tested concentrations. All Salmonella serotypes, except S. Diarizonae, showed excellent binding efficiency with purified anti-Salmonella pAbs. Moreover, the purified anti-Salmonella pAbs showed excellent specificity against all non-Salmonella strains. The anti-Salmonella pAbs immobilized on the gold biosensor demonstrated the successful capturing capability against Salmonella with a dose-response manner. Therefore, the anti-Salmonella pAbs exhibited sufficient reactivity, specificity, as well as capturing capability against Salmonella to be considered as a bio-recognition element.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38C no.7
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• pp.573-586
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• 2013

Recently, the research, utilizing bio and environment sensors in agricultural and cattle industries, are drawing attention among the various IT convergence technologies. Among such research IT convergence techniques which aim to improve the quality of cattle industry management are necessary in particular. Real-time monitoring of the cattles health condition, identification of the cause of the diseases and timely response to such epidemic based on IT convergence techniques are among them. In order to achieve the better management of cattle industry, we propose a cattles management system which based on various bio environment sensors and wireless network technologies. The system consists of wireless environmental sensor data acquisition nodes, sensor data processing nodes, the gateway and the server. The proposed system is able to actively monitor the cattle field, to respond quickly in the case of massive cattle diseases and to control the environment of the cattle fields. We believe the proposed system will demonstrate the successful application of IT technologies to an applied field such as farming industries because of real-time crisis management capability in case of epidemic and optimal management of cattle industry.

• Korean Journal of Environmental Biology
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• v.32 no.1
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• pp.49-57
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• 2014

Orb-web spiders can be considered as an effective bio-indicative animal to monitor the ecological air pollution of certain habitat since they have limited shifting ability during their life spans. In this study we revealed the fine structural characteristics of booklung in the orb-web spider Nephila clavata, and its bio-indicative significance in the monitoring environmental asbestos exposure. The high resolution scanning electron microscopy results suggest that the cuticular spikes near the atrium could be act as an effective filtering structure for dust materials. Furthermore, inner cuticular spikes which protruded across the air sacs, have the functions not only sustaining appropriate respiratory volume of each air sac but also help to enhance respiratory capacity by maximizing the gross surface area for gas exchange. Interestingly, in spite of short exposure, the asbestose fibrils were effectively captured on the surface of the atrial cuticular spikes. Furthermore, histologic damages were observed due to penetration of asbestos fibrils through air sacs and fixed on the hemolymph space. In addition, although accumulated mass of hemocytes were observed near the fibrils, there were no asbestos bodies or coagulated hemocytes were found on the surface of the fibrils, This could be mainly due to the short exposure period towards asbestos. Briefly, these results indicate the spider's booklung could be valuable tool in the detection ecological air pollutants.

• Journal of Wetlands Research
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• v.20 no.4
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• pp.314-321
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• 2018

In order to quantify nonpoint source pollution, it was proposed to sample at regular intervals of 1 hour for the first 24 hours of storm runoff process by National Institute of Environmental Research for the mixed landuse watershed. However, high frequency sampling requires intensive laboratory analysis and labor costs. In order to investigate the effect of longer sampling interval on the load estimation compared to the 1 hour sampling method, analysis was conducted using monitoring data from rural subwatershed, urban subwatershed, and outlet of the Pungyeongjeongcheon watershed. Statistical analysis revealed that mean of load estimation was not significantly different up to 4 hour sampling frequency. However, 3 hour sampling interval was found to be appropriate for the BOD and TP when it is judged that 10% or less of the difference in loading amount between the 1 hour and other sampling interval is reasonable. The results of this study can be used to conduct an effective monitoring system.

• Analytical Science and Technology
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• v.20 no.2
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• pp.138-146
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• 2007

A simple and sensitive method for the determination of paroxetine in canine plasma was developed and validated by liquid-liquid extraction and liquid chromatography-tandem mass spectrometry (LC-/MS/MS). Fluoxetine was used as an internal standard. Paroxetine and internal standard in plasma samples were extracted using TBME (tert-butyl methyl ether). A centrifuged upper layer was then evaporated and reconstituted with mobile phase of 50% acetonitrile adjusted to pH 3 by formic acid. The reconstituted samples were injected into a Capcell Pak UG120 ($2.0{\times}150mm$, $5{\mu}m$) column. Using MS/MS with SRM (selective reaction monitoring) mode, the transitions (precursor to product) monitored were m/z $330{\rightarrow}192$ for paroxetine, and m/z $310{\rightarrow}148$ for internal standard. Linear detection responses were obtained for paroxetine concentration range of 0.02~5 ng/mL. A correlation coefficient of linear regression ($R^2$) was 0.9993. Detection of paroxetine in canine plasma was accurate and precise, with limit of quantification at 0.02 ng/mL. The method has been successfully applied to pharmacokinetic study of paroxetine in healthy beagle dogs.

• Journal of Wetlands Research
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• v.26 no.3
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• pp.219-226
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• 2024

Payment for Ecosystem Services (PES) is emphasized to enhance ecosystem conservation and increase its ecological value. However, effective implementation of PES and policy execution requires insufficient ecosystem monitoring and research. Therefore, this study analyzed the effectiveness of PES implemented in Janghang Wetland to propose habitat management strategies. The study included monitoring migratory birds and analyzing key species' food sources. The dominant avian species observed in Janghang Wetland include Larus crassirostris, Anas platyrhynchos, Anser albifrons with their primary food sources analyzed as Gramineae plants such as Bromus japonicus, Elymus sibiricus, Brassicaceae plants such as Rorippa palustris. Furthermore, this study found that PES facilitates rice seed supply within Janghang Wetland, benefiting birds including Grus vipio, Anser fabalis, A. albifrons. To sustainable manage Janghang Wetland, improving food supply systems and exploring decentralized supply methods for G. vipio and Gooses (Anser fabalis, A. albifrons) are necessary. Additionally, managing boundaries between agricultural and developmental areas to improve ecological connectivity is essential. This study reaffirmed the ecological importance of Janghang Wetland as a crucial habitat for migratory species. The result will be significant as foundational data that can be used for future policy-making and support sustainable conservation efforts.

• Journal of the Society of Disaster Information
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• v.10 no.3
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• pp.374-387
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• 2014

Since the sexual violence crime has a high probability of repeated crime, the electronic monitoring system has been introduced as a measure to it. And this system allows the police to know the location of former criminal around the clock through the electronic device, the former criminal has the psychological/mental oppression which can restrain the intention of crime to a degree. However, there is a limit in blocking criminals with strong will from repeated crime. The next-generation intelligent electronic anklet currently under study collects and analyzes the change bio-data in real time through the location information of electronic monitoring target and attached sensor. This study is aimed to predict the symptom of crime occurrence in advance based on this and block the crime intention in advance or stop the ongoing crime before it is expanded.

• The Journal of the Korea Contents Association
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• v.11 no.5
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• pp.57-66
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• 2011

In recent years, health management services have been increased according to the increase in interests on health and the development of information technology (IT) based on increases in advanced ages and chronic disease patients. Thus, it requires the monitoring of health conditions and the specialized healthcare services not only in a hospital but also their own home. This study provides the specific notification services related to the context information based on users' bio signal data and the notification services of specific patients and attempts to develop a hypertension monitoring system and a notification service system according to indexes. Because this system considers the context of users by differing it from the conventional monitoring services, it makes possible to obtain more exact measurement values. In addition, it is able to reduce certain health risks through managing specific patients and based on living indexes. Also, it can provide more customized services to users due to the exact and finely classified services.

• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.2
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• pp.86-101
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• 2004

This study was conducted to present the ecological management of Goduk Riverside Restoration Area in Hangang, Seoul by analyzing the change of the vegetation structure. The survey site was classified into three groups. These were the vegetation restoration area, the dry plant area, and the swampy plant area. There were 141 taxa including naturalized plants and 13 species recorded in 2001 and 258 taxa including naturalized plants and 42 species were recorded in 2003 by monitoring. Monitoring results showed that the alien plants such as Humulus japonicus expanded continuously except in the vegetation restoration area. It was found that the growing status of planted shrub plants were poor, and the naturalized plants status was thriving, and the soil environment was bad in the vegetation restoration area. The alien plants such as Humulus japonicus and Aster pilosus dominated continuously in the dry plant area. The swampy native herb plants number was decreased, but the Humulus japonicus community was expanded caused by the soil drying in the swampy plant area. Soil analysis showed that the soil acidity, the available phosphates and the concentration of calcium were highly effected by cultivation. We propose ecological management as follows based on the results of the change of vegetation and soil characteristics. The vegetation restoration area should be managed by visitor's characteristics. Replanting vegetations should be based on soil characteristics. The removal of naturalized plants and established monitoring with plots is also needed. In the dry plant area and the swampy plant area, naturalized plants need to be removed in order to facilitate bio-diversity and monitoring.

• Journal of Plant Biotechnology
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• v.43 no.4
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• pp.479-485
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• 2016

Living modified (LM) crops are imported each year to South Korea as food and feeds, LM canola being one of the imported crops. The cultivation of LM crops is not permitted in South Korea but the import of these crops is increasing. In this study, we surveyed the environmental risk of imported LM canola at 9 provinces, from March 2009 to June 2013. Monitoring of canola was conducted around feed factories, roadsides, harbors, farmhouses, and flower festival regions. From the total of 595 canola samples collected from 1850 monitoring sites, we identified 6 LM canola samples. The LM canola samples were subjected to protein and DNA based analysis. PCR analyses using approved 5 single event primers (T45, MS8, RT73, Rf3 and Topas 19-2) revealed that two crops were glyphosate-resistant LM canolas, and four were glufosinate-resistant LM canolas. This study suggested that environmental monitoring is a useful research tool to manage LM crops unintentionally introduced into the environment in South Korea. This result can be used as a basis for future post-management of canola crops.