• 한국작물학회:학술대회논문집
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• 한국작물학회 2007년도 6th OSONG INTERNATIONAL BIO-SYMPOSIUM
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• pp.235.2-235.2
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• 2007

• 한국산학기술학회논문지
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• 제18권1호
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• pp.238-244
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• 2017

공작기계 응용에 있어서 고속 및 고효율 가공의 추세는 스핀들의 고속화를 지속적으로 요구하고 있다. 내장형 모터를 장착한 스핀들은 가공시스템의 구조를 단순하게 한다. 하지만 로터에는 불평형 질량에 의해 진동이 발생하며, 이를 제거하기 위한 밸런싱 작업은 필수적이다. 이 논문에서는 내장형 모터를 장착한 고속 스핀들의 동적거동을 해석하였다. 불평형 질량, 베어링 강성, 회전 속도의 변화에 따른 휘돌림 궤적을 해석하였고, 이를 저감시키는 방법을 모색하였다. 또한 Timoshenko 빔 요소를 적용하여 스핀들-베어링 시스템을 모델링하고, 영향 계수법을 적용하여 밸런싱 과정을 시뮬레이션 하였다. 스핀들의 경우, 불평형 하중이 작용할 때, 베어링 지지점에서 가장 작은 휘돌림 궤적이 나타났으며, 양단에서 가장 큰 휘돌림 궤적을 나타내었다. 스핀들의 회전속도가 증가함에 따라 스핀들 선단에서의 휘돌림 궤적도 증가하였다. 베어링의 강성이 커짐에 따라 휘돌림 궤적 또한 증가하였다. 밸런싱 전, 후의 휘돌림 궤적 반경은 최대 73%까지 감소함을 확인할 수 있었다. 이러한 연구 결과는 CNC 자동선반의 스핀들 고속화에 중요한 정보를 제공하고 있다.

• Mass Spectrometry Letters
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• 제3권1호
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• pp.18-20
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• 2012

We report observation of laser desorption/ionization (LDI) of peptides from flat surfaces of tungsten silicide ($WSi_2$). In contrast to MALDI (matrix-assisted laser desorption/ionization) and SALDI (surface-assisted laser desorption/ionization) mass spectrometry, this study did not utilize any matrices and surface nanostructures. In this work, LDI on $WSi_2$ surfaces is demonstrated to cover a mass range up to 1,600 Da (somatostatin; monoisotopic mass = 1637.9 Da). In addition, it exhibited a high sensitivity, which could detect peptides, which could detect peptides of low femtomole levels (20 fmol for angiotensin II). The observed LDI process was discussed to be largely thermal, more specifically, due to laser-induced surface heating that is most likely promoted by the low thermal diffusivity (${\kappa}$) of $WSi_2$ substrate.

• Mass Spectrometry Letters
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• 제6권4호
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• pp.85-90
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• 2015

This article reviews recent analytical techniques using inductively coupled plasma-mass spectrometry (ICP-MS) immunoassay for clinical and bio analysis. We classified the techniques into two categories, direct and indirect analysis, which depend upon a guideline of whether tagging materials are used or not. Direct analysis is well known, and generally used in conjunction with various other techniques, such as laser ablation, chromatographic separations, etc. Recently, indirect analysis using tagging elements has intensively been discussed because of its importance in future applications to bio and clinical analysis, including environmental and food industries. The method has shown advantages of multiplex detection, excellent sensitivity, and short analysis time owing to signal amplification and magnetic separation. Now, it expands the application field from small biomolecules to large cells.

• Interdisciplinary Bio Central
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• 제1권3호
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• pp.9.1-9.6
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• 2009

Introduction: In the mass spectrometry-based proteomics, biological samples are analyzed to identify proteins by mass spectrometer and database search. Database search is the process to select the best matches to the experimental mass spectra among the amino acid sequence database and we identify the protein as the matched sequence. The match score is defined to find the matches from the database and declare the highest scored hit as the most probable protein. According to the score definition, search result varies. In this study, the difference among search results of different search engines or different databases was investigated, in order to suggest a better way to identify more proteins with higher reliability. Materials and Methods: The protein extract of human mesenchymal stem cell was separated by several bands by one-dimensional electrophorysis. One-dimensional gel was excised one by one, digested by trypsin and analyzed by a mass spectrometer, FT LTQ. The tandem mass (MS/MS) spectra of peptide ions were applied to the database search of X!Tandem, Mascot and Sequest search engines with IPI human database and SwissProt database. The search result was filtered by several threshold probability values of the Trans-Proteomic Pipeline (TPP) of the Institute for Systems Biology. The analysis of the output which was generated from TPP was performed. Results and Discussion: For each MS/MS spectrum, the peptide sequences which were identified from different conditions such as search engines, threshold probability, and sequence database were compared. The main difference of peptide identification at high threshold probability was caused by not the difference of sequence database but the difference of the score. As the threshold probability decreases, the missed peptides appeared. Conversely, in the extremely high threshold level, we missed many true assignments. Conclusion and Prospects: The different identification result of the search engines was mainly caused by the different scoring algorithms. Usually in proteomics high-scored peptides are selected and low-scored peptides are discarded. Many of them are true negatives. By integrating the search results from different parameter and different search engines, the protein identification process can be improved.

• 한국임상수의학회지
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• 제28권4호
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• pp.435-437
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• 2011

2살령, 단모종의 집고양이가 2년 동안 만성 중이염과 콧물증상을 보였다. 구강 검사시 코인두에 종괴가 발견 되었다. 추가적인 검사를 위해, 컴퓨터 단층촬영술을 시행하였고 코인두 부분에서 큰 폴립이 발견되었다. Spay hook을 이용하여 폴립의 당김제거술을 수행하였다. 종괴를 제거한 후, 호르너증후군이 나타났으나 14일 이내에 자발적으로 해소되었다.

• Journal of the Optical Society of Korea
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• 제17권1호
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• pp.91-96
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• 2013

We report a method for optical monitoring of tumors in an animal model using optical coherence tomography (OCT). In a spectral domain OCT system, a superluminescent diode light source with a full width of 66 nm at half maximum and peak wavelength of 950 nm was used to take images having an axial resolution of 6.8 ${\mu}m$. Cancer cells of PC-3 were cultured and inoculated into the hypodermis of auricle tissues in BALB/c nude mice. We observed tumor formation and growth at the injection region of cancer cells in vivo and obtained the images of tumor mass center and sparse circumferences. On the $5^{th}$ day from an inoculation of cancer cells, histological images of the tumor region using cross-sectional slicing and dye staining of specimens were taken in order to confirm the correlation with the high resolution OCT images. The OCT image of tumor mass compared with normal tissues was analyzed using its A-scan data so as to obtain a tissue attenuation rate which increases according to tumor growth.

• Journal of Biosystems Engineering
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• 제33권2호
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• pp.101-105
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• 2008

Some physical properties of rapeseed such as geometric properties (linear dimensions, sphericity, seed volume, surface area) and gravimetric properties (the mass of one thousand seeds, bulk density) were analyzed at five levels of moisture content of 10.03, 14.91, 20.07, 25.06 and 30.12% (w.b.). The physical properties of rapeseed were evaluated as a function of seed moisture content. In the moisture range, when the moisture content increase, sphericity decreased from 0.946 to 0.927, and geometric mean diameter, seed volume and surface area increased from 2.17 to 2.31 mm, 5.58 to $6.88 \;mm^3$ and 14.76 to $16.77\;mm^2$ respectively. Mass of one thousand seeds increased from 5.04 to 6.46 g. Bulk density decreased from 579.3 to $549.2\;kg/m^3$ due to swelling of the seed.

• Natural Product Sciences
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• 제24권1호
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• pp.71-78
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• 2018

The present study deals with the determination of optimal values of operating parameters such as the temperature of heating, the mass of the plant material and the volume of water leading to the best yield of electromagnetic induction (EMI) heating extraction of Algerian Thymus fontanesii essential oil. After an appropriate choice of the three critical variables, eight experiments leaded to a mathematical model as a first-degree polynomial presenting the response function (yield) in the relation to the operating parameters. From the retained model, we were able to calculate the average response, the different effects and their interactions. The maximum of essential oil recovery percentage relative to the initial mass of plant material was 1.69%, and was obtained at ($140^{\circ}C$, 250 g and 4.5 L). The chemical composition of the Algerian T. fontanesii essential oil under the obtained optimal conditions ($140^{\circ}C$, 250 g and 4.5 L), determined by GC/MS and GC/FID, reveled of the presence of major components such as: carvacrol ($70.6{\pm}0.1%$), followed by p-cymene ($8.2{\pm}0.2%$).

• Journal of Microbiology and Biotechnology
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• 제7권6호
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• pp.417-422
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• 1997

Recombinant Bacillus subtilis LKS88[pASA240] containing the amylase gene from Streptomyces albus KSM-35 was exploited in fed-batch cultivation for mass production of maltotetraose-producing amylase. The effects of dissolved oxygen, additional organic nutrients (peptone and yeast extract) and mixed carbon sources (glucose plus soluble starch) on amylase production were examined in fed-batch operations in an effort to determine the optimum conditions for a maximum amylase productivity. Under the optimum conditions, maximum amylase activity was about 4.2 times higher than that obtained in batch cultivations, indicating that mass production of maltotetraose-producing amylase could be accomplished in fed-batch cultivation of the recombinant B. subtilis strain.