• Journal of the Korean Magnetic Resonance Society
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• v.16 no.2
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• pp.147-161
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• 2012

Human melanocortin-4 receptor (hMC4R) among MC-Rs, expressed in the brain, is in charge of the control on energy homeostasis and food intake. The structure and function of human MC4R have been studied to understand their essential function and roles. To investigate the structure and function, it is necessary to prepare sufficient amounts of proteins. However, their expression and purification is demanding and time-consuming due to their innate insoluble and toxic properties. The heterozygous mutations of hMC4R, exchange of Asp 90 to Asn located in second transmembrane, cause severe obesity in human. To obtain purified hMC4R wt-TM2 for structural studies, it was first over-expressed and purified by fast protein liquid chromatography (FPLC) and then solution NMR studies were performed to get high-resolution spectra. In here, we established optimized purification scheme to get more purified target peptide.

• YAKHAK HOEJI
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• v.48 no.3
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• pp.213-217
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• 2004

For the determination of glutathione S-transferase activity, a new method was established by using HPLC system. Moreover, amount of enzyme for a optimum reaction was determined by a comparative study with a variety concentration of enzyme. Using a established method, activity of glutathione S-transferase that is alcohol metabolizing enzyme was investigated on the fruit of Hovenia dulcis Thunb. As the result of experiment, EtOH and $H_2O$ extracts of the fruit of Hovenia dulcis Thunb showed visible a synergistic effect of glutathione S-transferase activity. On a continuous experiment, EtOH and $H_2O$ extracts of the fruit of Hovenia dulcis Thunb showed alcohol decomposition activity on the in vivo test using rat. These results suggest that the fruit of Hovenia dulcis Thunb may be useful in the prevention of hangover.

• Asia Marketing Journal
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• v.6 no.3
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• pp.157-171
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• 2004

The objective of this case is to introduce KT&G's marketing strategy to go beyond its crisis. KT&G's crisis has started from tobacco which is its cash cow. Tobacco has been criticized as one of cancer causes. Until now Go and NGO suppress smoking. Its crisis is non-smoking. To live and grow beyond this crisis KT&G has 4 marketing strategies. First, they introduce new product tobacco by segmenting smoking market. Second, they want to get strong brand which has high quality, price premium, and favorable image. Third, they try to connect tobacco and bio-industry. Tobacco is very important resources of bio-industry. Finally, they is going to enter well-being market by its strength which is ginseng.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2021.05a
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• pp.499-501
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• 2021

The steady investment and development in the fourth industry led to the development of IoT technology, which led to the dissemination of platform-based blockchain technology that could provide the necessary security platform. BIoT, a blockchain-based IoT application, requires blockchain platform technology that provides more sophisticated security, scalability, and efficiency in order to be applied to more diverse fields in the future. In this paper, we analyze the recently announced smart contract ETH 2.0 to compare the requirements for existing BIoT development and to predict the future of BIoT in the future.

• The Korean Journal of Food & Health Convergence
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• v.9 no.5
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• pp.7-11
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• 2023

Purpose: The purpose of this study is to analyze current trends in the Korean ice cream industry and determine how these trends affect the competitiveness of each ice cream company. Method: This study was conducted by reviewing existing related literature and collecting and analyzing data from news articles, corporate reports, and existing researchers' studies related to the ice cream industry. Results: The Korean ice cream industry is growing and developing through the collaboration of fun marketing with taste, quality, and fun elements, and the young MZ generation can be said to be the main customer base. In terms of quality, premium ice cream made with high-quality domestic ingredients has recently been gaining popularity, and ice cream with reduced fat and sugar, such as plant-based substitutes, is also gaining popularity among consumers. Conclusions and Implications: The results of this study highlight the need for continued innovation in the Korean ice cream industry. The industry must also capitalize on the growth of online sales and the global appeal of Korean pop culture. Sustainability efforts, technology adoption, and strategic collaboration are key drivers for future industry success, and social media engagement must be integrated into marketing strategies to increase brand awareness and consumer loyalty.

• Journal of the Korea Furniture Society
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• v.23 no.3
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• pp.298-304
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• 2012

Since 1990's, a flooring based on plywood has gotten customers' demand. Costs of raw material and production increased because of changed environment of industry. Tropical timber such as Red Meranti (Shorea acuminate) used for raw material of the floor has been depleting beside countries in South Eastern Asia changed species of afforestation. As a result, it gets hard to secure good quality of raw material for plywood. Moreover plywood price is increased suddenly after earthquake in Japan. Eucalyptus (Eucalyptus globulus) in china has been using for raw material as a countermeasure of changed environment of industry. In this study, possibility of using flooring consisted of Eucalyptus veneer as a core layer was checked by heat transfer experiments. Flooring consisted of Red Meranti was used for comparison. Two factors which impact on heat transfer are a type of density gradient and density difference between Long-grain veneer and Short-grain veneer. Red Meranti samples are 'M' type of density gradient on the other hand Eucalyptus samples are 'W' type of density gradient.

• Journal of the Korean Society of Industry Convergence
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• v.25 no.2_2
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• pp.309-314
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• 2022

The bonding strength [ISO22674] test was conducted by firing a dedicated ceramic powder on the surface of the dental titanium material treated with micro-arc oxidation. In the test group, an average result value of 34.34 MPa was obtained, and in the control group, a result value of 21.53 MPa was obtained. The bonding strength of the test group was higher than that of the control group by 12.81 MPa, resulting in a 37% improvement in durability of the dental artificial tooth ceramic restoration.

• Journal of Microbiology and Biotechnology
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• v.33 no.10
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• pp.1361-1369
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• 2023

Corynebacterium glutamicum (C. glutamicum) has been considered a very important and meaningful industrial microorganism for the production of amino acids worldwide. To produce amino acids, cells require nicotinamide adenine dinucleotide phosphate (NADPH), which is a biological reducing agent. The pentose phosphate pathway (PPP) can supply NADPH in cells via the 6-phosphogluconate dehydrogenase (6PGD) enzyme, which is an oxidoreductase that converts 6-phosphogluconate (6PG) to ribulose 5-phosphate (Ru5P), to produce NADPH. In this study, we identified the crystal structure of 6PGD_apo and 6PGD_NADP from C. glutamicum ATCC 13032 (Cg6PGD) and reported our biological research based on this structure. We identified the substrate binding site and co-factor binding site of Cg6PGD, which are crucial for understanding this enzyme. Based on the findings of our research, Cg6PGD is expected to be used as a NADPH resource in the food industry and as a drug target in the pharmaceutical industry.

• Environmental Engineering Research
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• v.25 no.1
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• pp.18-28
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• 2020

In this paper the authors provide comparative evaluation of current research that used liquefaction and pyrolysis method for bio-oil production from various types of biomass. This paper review the resources of biomass, composition of biomass, properties of bio-oil from various biomass and also the utilizations of bio-oil in industry. The primary objective of this review article is to gather all recent data about production of bio-oil by using liquefaction and pyrolysis method and their yield and properties from different types of biomass from previous research. Shortage of fossil fuels as well as environmental concern has encouraged governments to focus on renewable energy resources. Biomass is regarded as an alternative to replace fossil fuels. There are several thermo-chemical conversion processes used to transform biomass into useful products, however in this review article the focus has been made on liquefaction and pyrolysis method because the liquid obtained which is known as bio-oil is the main interest in this review article. Bio-oil contains hundreds of chemical compound mainly phenol groups which make it suitable to be used as a replacement for fossil fuels.

• Journal of Life Science
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• v.28 no.2
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• pp.216-222
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• 2018

Desmodium heterocarpon is one of vines belongs to Fabaceae family, mainly distributed in Asian countries such as Korea and Japan. This study was conducted to explore new nutraceutical resources from the plant kingdom possessing biological activities. To fulfill this purpose, the anti-oxidative and anti-inflammatory activities of D. heterocarpon ethanol extract (DHEE) were evaluated by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity assay, reactive oxygen species (ROS) scavenging activity assay, nitric oxide (NO) inhibitory activity assay, and the analysis of related protein expressions by Western blot hybridization. DHEE exhibited potent anti-oxidative activity as confirmed by DPPH radical scavenging capacity against DPPH similar with ascorbic acid, a well-known anti-oxidative agent, used as a positive control. DHEE also effectively suppressed hydrogen peroxide ($H_2O_2$)-induced ROS on RAW 264.7 murine macrophage cells. Furthermore, DHEE induced the expression of the anti-oxidative enzyme heme oxygenase 1 (HO-1), and its upstream transcription factor, nuclear factor-E2-related factor 2 (Nrf2) as a dose dependent manner. DHEE inhibited lipopolysaccharide (LPS) induced nitric oxide (NO) formation as a consequence of inducible NO synthase (iNOS) down regulation. Taken together, these results suggest that DHEE has anti-oxidative and anti-inflammatory activities and thus appears to be useful sources as potential anti-oxidant and anti-inflammatory agents. The identification of active compounds that confer biological activities of DHEE might be needed.