• KSBB Journal
• /
• v.31 no.1
• /
• pp.33-39
• /
• 2016

Developing the method for the selection of animal cell line producing therapeutic monoclonal antibody (mAb) is invaluable as its market is rapidly growing. Although the quality of produced mAb is as important as quantity, however there is no method developed for the selective screening of cell lines on the basis of both quantity and quality. From recent reports, the ratio of light and heavy chain mRNAs of mAb in the cell is a key parameter for the indication of product quality. Therefore, it is obvious that developing the novel method that can detect both light and heavy chain mRNAs in single live cell will provide unprecedented opportunities in bio-industry. Here, we have constructed oligonucleotide probes, molecular beacons for the detection of light or heavy chain mRNAs, respectively, in the live cells producing mAbs. Both beacons showed increased fluorescent intensity after transient transfection of plasmid expressing mAbs analyzed by fluorometer. Flow cytometric analysis clearly demonstrated that both molecular beacons can simultaneously detect the expression of light and heavy chain mRNAs of mAb in the same cell. The technique described in the thesis provides the new direction and concept for developing the method for the smart selection of cell lines producing recombinant proteins including therapeutic mAbs.

• Applied Biological Chemistry
• /
• v.50 no.1
• /
• pp.1-5
• /
• 2007

For the purpose of application for biomass of Pleurotus eryngii, the optimum culture condition were tested. It was found that the optimum culture condition for spot culture of pleurotus eryngii were 24$^{\circ}C$ for 18 days with PDA medium. And the optimum culture condition of bioreactor for biomass were pH 5.5, 18$^{\circ}C$ and 27 days with PDMP broth. It was possible to artificial cultivation of mycelial from Pleurotus eryngii using bioreactor for biomass under the optimum conditions, and it was also possible for Pleurotus eryngii biomass because the forming of fruiting body when Pleurotus eryngii was cultivated using mass artificial cultivated mycelial in the bioreactor.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2006.06a
• /
• pp.390-391
• /
• 2006

This paper describes the design, fabrication and characteristics of a micromachined piezoelectric valve utilizing a multilayer ceramic actuator (MCA). The micromachined MCA valve, which uses a buckling effect, consists of three separate structures: the MCA, the valve actuator die and the seat die. The valve seat die with 6 trenches was made, and the actuator die, which is driven by the MCA under optimized conditions, was also fabricated. After Si wafer direct bonding between the seat die and the actuator die, the MCA was also anodically bonded to the seat/actuator die structure. A polydimethylsiloxane (PDMS) sealing pad was fabricated to minimize the leak rate. Finally, the PDMS sealing pad was also bonded to the seat die and the stainless steel package. The MCA valve shows a flow rate of 9.13 sccm at an applied DC voltage of 100 V with a 50% duty cycle and a maximum non-linearity of 2.24% FS. Therefore, the fabricated MCA valve is suitable for a variety of flow control equipment, as a medical bio-system and in the automobile industry.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.26 no.10
• /
• pp.1982-1988
• /
• 2002

Micro-injection molding and microfluidic devices with the help of MEMS technologies including the LIGA process are expected to play important roles in micro-system industries, in particular the bio-application industry, in the near future. Understanding fluid flows in micro-channels is important since micro-channels are typical geometry in various microfluidic devices and mold inserts for micro-injection molding. In the present study, Part 1, an experimental investigation has been carried out to understand the detailed flow phenomena in micro-channel filling process. Three sets of micro-channels of different thickness (40um,30um and 2011m) were fabricated using SU-8 on silicon wafer substrate. And a flow visualization system was developed to observe the filling flow into the micro-channels. Experimental flow observations are extensively made to find the effects of pressure, inertia force, viscous force and surface tension. A dimensional analysis for experimental results was carried out and several relationships A dimensionless parameters are obtained.

• Journal of the Korean Magnetic Resonance Society
• /
• v.13 no.2
• /
• pp.117-125
• /
• 2009

HP0062 is an 86 residue hypothetical protein from Helicobacter pylori strain 26695. HP0062 was identified ESAT-6/WXG100 superfamily protein based on structure and sequence alignment and also contains leucine zipper domain sequence. Here, we report the sequence-specific backbone resonance assignment of HP0062. About 97.7% of all $^1H_N,\;^{15}N,\;^{13}C_{\alpha},\;^{13}C_{\beta}\;and\;^{13}C=O$ resonances were assigned unambiguously. We could predict the secondary structure of HP0062 by analyzing the deviation of the $^{13}C_{alpha}\;and\;^{13}C_{\beta}$ chemical shifts from their respective random coil values. Secondary structure prediction shows that HP0062 consist of two ${\alpha}$-helices. This study is a prerequisite for determining the solution structure of HP0062 and can be used for the study on interaction between HP0062 and DNA and other Helicobacter pylori proteins.

• Journal of agriculture & life science
• /
• v.44 no.1
• /
• pp.9-15
• /
• 2010

Barley, a monocotyledonous plant, is relatively recalcitrant to the process of Agrobacterium-mediated genetic transformation. In this study, seedlings of six barley cultivars (Keunal-1-Ho, Saessal, Ol, Saechalssal, Seodunchal and Pungsanchalssal) were injured using alkali, oxidizing or reducing agents. They were then transformed using Agrobacterium via vacuum infiltration for the analysis of comparative GUS gene expression. It was determined that chemical injuries causing a slight growth retardation could overall enhance the GUS transformation rate. Hydrogen peroxide was determined to be the most effective.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.27 no.4
• /
• pp.49-58
• /
• 2004

The R&D investment of Korean government has been concentrated into 6T areas-IT(Information Technology), BT(Bio-Technology), NT(Nano Technology), ET(Environment & Energy Technology), ST(Space Technology) and CT(Culture & Contents Technology) - for a couple of years. By this selection and concentration strategy, patent applications are on an increasing trend in these areas. This paper examined the trends of patent application in 6T areas. To do this, this paper classified each technology area into the detail technology area with the valid number of patent applications. According to the result, the analysis data will be used to make R&D budget appropriation of the government.

• Food Science and Preservation
• /
• v.17 no.5
• /
• pp.752-756
• /
• 2010

Lepidoptera (butterflies) extracts, traditionally employed as medicines, have various biological activities. Five species of Lepidoptera (Papilio maackii, Papilio xuthus, Pieris rapae, Eurema hecabe, and Sasakia charonda) were extracted with distilled water (DW), dimethyl sulfoxide (DMSO), ethanol (EtOH), and methanol (MeOH). Each extract was analyzed for anti-oxidant and anti-inflammatory activities using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay method, the ferric reducing ability of plasma (FRAP) test, and a cyclooxygenase-2 (COX-2) promoter assay. The results suggest that Lepidoptera extracts have valuable anti-oxidant and anti-inflammatory properties, supporting the idea that the extracts may serve as a food biomaterial(s) preventing oxidative processes and inflammatory damage.

• Elastomers and Composites
• /
• v.56 no.3
• /
• pp.136-151
• /
• 2021

Biomass lignin, a waste produced during the paper and bio-ethanol production process, is a cheap material that is available in large quantities. Thus, the interest in the valorization of biomass lignin has been increasing in industrial and academic areas. Over the years, lignin has been predominantly burnt as fuel to run pulping plants. However, less than 2% of the available lignin has been utilized for producing specialty chemicals, such as dispersants, adhesives, surfactants, and other value-added products. The development of value-added lignin-derived co-products should help make second generation biorefineries and the paper industry more profitable by valorizing lignin. Another possible approach towards value-added applications is using lignin as a component in plastics. However, blending lignin with polymers is not simple because the polarity of lignin molecules results in strong self-interactions. Therefore, achieving in-depth insights on lignin characteristics and structure will help in accelerating the development of lignin-based products. Considering the multipurpose characteristics of lignin for producing value-added products, this review will shed light on the potential applications of lignin and lignin-based derivatives on polymeric composite production. Moreover, the challenges in lignin valorization will be addressed.

• Korean Journal of Ichthyology
• /
• v.31 no.2
• /
• pp.83-89
• /
• 2019

The effect of estuarine dams on fish assembladge in Danghang Bay of Goseong, Korea was determined using monthly samples collected by a beam trawl and a cast net at the natural estuaries and damed estuary from September 2011 to August 2012. In the natural estuaries without dam, salinity was relatively high showing a large diurnal change. Estuarine fishes such as striped sandgoby, Acentrogobius pflaumii and yellowfin goby, Acanthogobius flavimanus, were dominantly caught in the sea side water. In the dammed estuary, freshwater species such as pale chub, Zacco platypus and triden goby, Tridentiger brevispinis were dominanlty caught. The dam in the Danghang estuary have significant effects on the fish assemblage through aquatic environmental change such as salinity.