• Journal of the Korean Magnetic Resonance Society
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• v.20 no.2
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• pp.36-40
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• 2016

MRA1997 is a highly conserved protein from mycobacterial strains. However, no structural and functional information is associated with it. Thus, to obtain details about structure and function of this protein, we have utilized NMR spectroscopy. The recombinant MRA1997 was highly purified and its DNA binding mode was characterized. The tertiary structure of MRA1997 was modeled on the basis of our NMR chemical shift data combined with the webserver CS23D. The binding of MRA1997 with DNA was first monitored by electrophoresis mobility shift assays. The residues involved in DNA binding are identified using NMR chemical shift perturbation experiments. Based on our study, we suggest that MRA1997 interacts with DNA and may play an important role in Mycobacterium tuberculosis physiology.

• Journal of Information Technology Services
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• v.15 no.1
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• pp.1-18
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• 2016

Recently, in the bio-field success stories of ventures through a biosimilar technology is being excavated. but the growth of ICT industry has been stagnant since reaching a boom in the dissemination of early high-speed internet in 2000s. The purpose of this study is to explore the factors of change of business model and business strategy of ICT ventures and SMEs with the evolution of the digital ecosystem, and to drive the factors to be competitive on the global value chain. The researcher selected an entreprenuership, market-innovation orientation, technology-innovation orientation, and Administration-innovation orientation as internal factors influencing the global competence and healthiness of the ecosystem as external factors. The researcher applied samples of 94 ICT Venture and SMEs to a research model, and adopted 5 hypotheses. The researcher believes that only a few hypotheses were adopted because it takes time for overall innovation orientation of ICT Venture and SMEs to result in the real global competence as the their innovation orientation is still on the level of domestic market. And the researcher also thinks that only healthiness of the ecosystem affected management performances because the companies' performances of the last 3 years were so weak that the correlation between innovation orientation of each company and the performances were not big enough.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.17 no.5
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• pp.515-520
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• 2004

We report on the development of a Piezoelectric valvc that is designed to have a high reliability for fluid control systems, such as mass flow control, transportation and chemical analysis. The valve was fabricated using a MCA(multilayer ceramic actuator), which has a low consumption power, high resolution and accurate control. The fabricated valve is composed of MCA, a valve actuator die and an seat die. The design of the actuator dic was done by FEM(finite element method) modeling, respectively. And, the valve seat die with 6 trenches was made. and the actuator die, which possible to optimize control to MCA, was fabricated. After Si-wafer direct bonding between the seat die and the actuator die, MCA was also anodic bonded to the scat/actuator die structure. PDMS(poly dimethylsiloxane) sealing pad was fabricated to minimize a leak-rate. It was also bonded to scat die and stainless steel package. The flow rate was 9.13 sccm at a supplied voltage of 100 V with a 50 % duty ratio and non-linearity was 2.24 % FS. From these results, the fabricated MCA valve is suitable for a variety of flow control equipments, a medical bio-system, semiconductor fabrication process, automobile and air transportation industry with low cost, batch recess and mass production.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.2
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• pp.163-169
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• 2003

The floating bead filter was tested for treatment of aquacultural water in a pilot-scale recirculating aquaculture system. Performance of floating bead filter on the removal of total suspended solids (TSS) and the treatment of nitrogen sourer such as total ammonia nitrogen (TAN), nitrite nitrogen and nitrate nitrogen were evaluated. The system was stocked with Nile tilapia at an initial rearing densities of $5\%\;and\;7\%$ over 30 days. The average TSS removal rates were $43.0\;g/m^2{\cdot}day\;and\;39.5\;g/m^2{\cdot}day$ for rearing density of $5\%\;and\;7\%$, respectively. As rearing density increased from $5\%\;to\;7\%$. the TAN removal efficiency decreased from $22.0\%\;to\;17.7\%$. At the rearing densities of $5\%\;and\;7\%$, the average TAN removal rates and removal efficiencies were $38.8\;g/m^2{\cdot}day,\;15.6\%\;and\;37.8\;g/m^2{\cdot}day.\;17.7\%,$ respectively. The average TAN removal rate was $37.8-38.8\;g/m^3{\cdot}day.$ The oxygen consumption by floating bead filter was higher than theoretical oxygen consumption rate by nitrification.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.3
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• pp.179-185
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• 2004

The characteristics of foam generation were assessed for freshwater and seawater using a foam generator. Both in freshwater and seawater, the height of the foam layer increased with initial protein concentrations. The height of the foam layer also increased with pore size of the air distributor. The optimum superficial air velocities (SAV) in freshwater and seawater were 0.84 cm/sec and 0.6 cm/sec, respectively. The height of the foam layer was the highest in pH 3 in freshwater and in the region of pH 5-7 in seawater. The height of the foam layer increased with $NaHCO_3$ concentration in freshwater, and $NaHCO_3$ concentration had little effect in seawater. Removal efficiencies of total suspended solid (TSS) and turbidity decreased with an increase of initial protein concentrations in a batch foam separator both in freshwater and seawater.

• Fisheries and Aquatic Sciences
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• v.13 no.3
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• pp.216-223
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• 2010

Abalone (Haliotis discus hannai), mostly distributed and maricultured in southwestern coastal areas of South Korea, is recognized as an economically important species in the fishery industry. Abalone intestines are one of the by-products of abalone processing. To investigate abalone intestines as bioactive substances, abalone intestine gastrointestinal digests (AIGIDs) of various molecular weights (MWs) were prepared using in vitro gastrointestinal digestion and an ultrafiltration system, and tested for inhibitory effects against reactive oxygen species (ROS) and oxidative stress in macrophage cells treated with hydrogen peroxide ($H_2O_2$). In our results, among AIGIDs, AIGID-III (MW=5-10 kDa) showed potent inhibitory activities for lipid peroxidation and free radicals. Additionally, the results clearly indicated that AIGID-III treatment could prevent cytotoxic damage of macrophages by $H_2O_2$-induced oxidative stress due to its potent scavenging ability against cellular ROS. These results suggest that AIGIDs may have protective and therapeutic potential for oxidative stress syndromes and immune diseases through ROS inhibition in macrophage cells.

• Korean Journal of Food Science and Technology
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• v.48 no.6
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• pp.618-621
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• 2016

Geranium thunbergii is a perennial plant commonly used as an oriental medicine for prevention of diarrhea, constipation, and gastrointestinal disorders. However, its anti-inflammatory effect has not been evaluated thus far. Therefore, the present study aimed to investigate the anti-inflammatory effect of G. thunbergii. In this study, G. thunbergii extracted with methanol; this methanol extract was further partitioned using various solvents, and G. thunbergii ethyl acetate fraction (GTEF) was obtained. To determine the anti-inflammatory activity of G. thunbergii, the effects of GTEF on nitric oxide (NO) production in lipopolysaccharide-stimulated RAW 264.7 cells were evaluated. GTEF suppressed NO production in a dose-dependent manner without any toxic effects. In addition, western blotting was performed to examine the effect of GTEF on expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggest that GTEF as a phytoextract may be useful for the prevention or treatment of inflammation.

• Laser Solutions
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• v.15 no.3
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• pp.1-6
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• 2012

The friction force of patterned needle in elastomer have been investigated to verify the application for bio and plastic industry. The micro pattern on the needle surface were prepared by 266 nm, 20 ns laser and 800 nm, 220 fs laser, which were able to generate the different surface roughness. The friction force was measured by the load cell of 10 N capacity. As the results, the friction force of no patterned needle is almost constant during the needle penetrates the silicone rubber sample. However, the needle having asperities shows the variation of the friction force. The higher the surface roughness is, the smaller the friction force is until the surface roughness is very high. In our experiment conditions, the reduction of the friction force by 20 % compared to no pattern needle was achieved with straight and $50{\mu}m$ discrete line generated by 266 nm, 20 ns laser.

• Food Science and Biotechnology
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• v.14 no.6
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• pp.836-840
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• 2005

Extraction characteristics of Bonus species of Brassica oleracea var. capita and functional properties of corresponding extract were monitored by response surface methodology (RSM). Maximum extraction yield of 44.07% was obtained at ratio of solvent to sample of 27.94 mL/g, ethanol concentration of 24.35%, and extraction temperature of $55.21^{\circ}C$. At ratio of solvent to sample, ethanol concentration, and extraction temperature of 21.11 mL/g, 58.53%, and $68.83^{\circ}C$, respectively, maximum electron-donating ability was 48.44%. Maximum inhibitory effect on tyrosinase was 68.94% at ratio of solvent to sample, ethanol concentration, and extraction temperature of 24.08 mL/g, 10.49%, and $78.71^{\circ}C$, respectively. Superoxide dismutase (SOD) showed maximum pseudo-activity of 24.78% at ratio of solvent to sample of 22.66 mL/g, ethanol concentration of 45.69%, and extraction temperature of $93.81^{\circ}C$. Based on superimposition of four-dimensional RSM with respect to extraction yield, electron-donating ability, and pseudo-activity of SOD, optimum ranges of extraction conditions were ratio of solvent to sample of 20-30 mL/g, ethanol concentration of 35-65%, and extraction temperature of $50-80^{\circ}C$.

• Fashion & Textile Research Journal
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• v.13 no.1
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• pp.1-6
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• 2011

Design patent is a type of industrial design right that is granted on the ornamental design of a functional item. We analyzed domestic design patents that were applied from 1965 until 2008, to the field of functional clothing. Since the year 2000 the number of design patents of functional clothing progressively increased. Design patents applied to the functional clothing field includes sport wear (47%), working clothes (34%), and leisure wear (19%). Design development of the sports wear from the latter half of 1990's has shown a continuously increasing tendency. The most patent applications belonged to the companies(51.4%), followed by individual applicants (46.6%), and the universities or laboratories had the least number of applications (2.0%). In case of working clothes or the leisure wear, the number of design applications focusing on the functional characteristic is higher than that of aesthetic characteristic, however, the number of design applications focusing on the aesthetic characteristic is higher in sportswear.