Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory effect of Geranium thunbergii on lipopolysaccharide-stimulated RAW 264.7 cells

  • Kwon, Tae-Hyung (Chuncheon Bioindustry Foundation) ;
  • Lee, Su-Jin (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, You-Jeong (Food Science and Biotechnology Major, Andong National University) ;
  • Park, Jung-Ja (Department of Food and Biotechnology, Hankyong National University) ;
  • Kim, Taewan (Food Science and Biotechnology Major, Andong National University) ;
  • Park, Nyun-Ho (Gyeongbuk Institute for Marine Bio-Industry)
  • Received : 2016.07.05
  • Accepted : 2016.08.09
  • Published : 2016.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Geranium thunbergii is a perennial plant commonly used as an oriental medicine for prevention of diarrhea, constipation, and gastrointestinal disorders. However, its anti-inflammatory effect has not been evaluated thus far. Therefore, the present study aimed to investigate the anti-inflammatory effect of G. thunbergii. In this study, G. thunbergii extracted with methanol; this methanol extract was further partitioned using various solvents, and G. thunbergii ethyl acetate fraction (GTEF) was obtained. To determine the anti-inflammatory activity of G. thunbergii, the effects of GTEF on nitric oxide (NO) production in lipopolysaccharide-stimulated RAW 264.7 cells were evaluated. GTEF suppressed NO production in a dose-dependent manner without any toxic effects. In addition, western blotting was performed to examine the effect of GTEF on expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggest that GTEF as a phytoextract may be useful for the prevention or treatment of inflammation.

Keywords

References

  1. Yang Y, Yu T, Jang HJ, Byeon SE, Song SY, Lee BH, Rhee MH, Kim TW, Lee JH, Hong SY, Cho JY. In vitro and in vivo antiinflammatory activities of Polygonum hydropiper methanol extract. J. Ethnopharmacol. 139: 616-625 (2012) https://doi.org/10.1016/j.jep.2011.12.003
  2. Kim EO, Min KJ, Won TK, Um BH, Moreau RA, Choi SY. Anti-inflammatory activity of hydroxycinnamic acid derivatives isolated from corn bran in lipopolysaccharide-stimulated RAW 264.7 macrophage. Food Chem. Toxicol. 50: 1309-1316 (2012) https://doi.org/10.1016/j.fct.2012.02.011
  3. Yu T, Lee YJ, Jang HJ, Kim AR, Hong SY, Kim TW, Kim MY, Lee JH, Lee YG, Cho JY. Anti-inflammatory activity of Sorbus commixta water extract and its molecular inhibitory mechanism. J. Ethnopharmacol. 134: 493-500 (2011) https://doi.org/10.1016/j.jep.2010.12.032
  4. Bharat Reddy D, Reddanna P. Chebulagic acid (CA) attenuates LPS-induced inflammation by suppressing NF-${\kappa}B$ and MAPK activation in RAW 264.7 macrophage. Biochem. Bioph. Res. Com. 381: 112-117 (2009) https://doi.org/10.1016/j.bbrc.2009.02.022
  5. Park YM, Won JH, Kim YH, Choi JW, Park HJ, Lee KT. In vivo and in vitro anti-inflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus. J. Ethnopharmacol. 101: 120-128 (2005) https://doi.org/10.1016/j.jep.2005.04.003
  6. Lee YG, Lee WM, Kim JY, Lee IK, Yun BS, Rhee MH, Cho JY. Src kinase-targeted anti-inflammatory activity davallialactone from Inonotus xeranticus in lipopolysaccharide-activated RAW 264.7 cells. Br. J. Pharmacol. 154: 852-863 (2008)
  7. Choi HJ, Choi HJ, Park MJ, Lee JY, Jeong SI, Lee S, Kim KH, Joo M, Jeong HS, Kim JE, Ha KT. The inhibitory effects of Geranium thunbergii on interferon-${\gamma}$-and LPS-induced inflammatory responses are mediated by Nrf2 activation. Int. J. Mol. Med. 35: 1237-1245 (2015) https://doi.org/10.3892/ijmm.2015.2128
  8. Sung YY, Yoon T, Yang WK, Kim SJ, Kim HK. Anti-obesity effects of Geranium thunbergii extract via improvement of lipid metabolism in high-fat diet-induced obese mice. Mol. Med. Rep. 4: 1107-1113 (2011)
  9. Yun K, Jun M. In vitro BACE1 inhibitory activity of geraniin and corilagin from Geranium thunbergii. Planta Med. 79: 1038-1042 (2013) https://doi.org/10.1055/s-0032-1328769
  10. Keiser K, Jhonson CC, Tipton DA. Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts. J. Endod. 26: 288-291 (2000) https://doi.org/10.1097/00004770-200005000-00010
  11. Wang BS, Huang GJ, Tai HM, Huang MS. Antioxidant and antiinflammatory activities of aqueous extracts of Schizonepeta tenuifolia Briq. Food Chem. Toxicol. 50: 526-531 (2012) https://doi.org/10.1016/j.fct.2011.12.010
  12. Pokharel YR, Liu QH, Oh JW, Woo ER, Kang KW. 4-Hydroxykobusin inhibits the induction of nitric oxide synthase by inhibiting NF-${\kappa}B$ and AP-1 activation. Biol. Pharm. Bull. 30: 1097-1101 (2007) https://doi.org/10.1248/bpb.30.1097
  13. Wu TT, Tsa CW, Wao HT, Lii CK, Chen HW, Wu YL, Chen PY, Liu KL. Suppressive effects of extracts from the aerial part of Coriandrum sativum L. on LPS-induced inflammatory responses in murine RAW 264.7 macrophages. J. Sci. Food Agric. 90: 1846-1854 (2010)
  14. Kang SR, Park KI, Park HS, Lee DH, Kim JA, Nagappan A, Kim EH, Lee WS, Shin SC, Park MK, Han DY, Kim GS. Antiinflammatory effect of flavonoids isolated from Korea Citrus aurantium L, on lipopolysaccharide-induced mouse macrophage RAW 264.7 cells by blocking of nuclear factor-kappa B (NF-${\kappa}B$) and mitogen-activated protein kinase (MAPK) signaling pathways. Food Chem. 129: 1721-1728 (2011) https://doi.org/10.1016/j.foodchem.2011.06.039
  15. Jeong JB, Jeong HJ. Rheosmin, a naturally occurring phenolic compound inhibits LPS-induced iNOS and COX-2 expression in RAW 264.7 cells by blocking NF-${\kappa}B$ activation pathway. Food Chem. Toxicol. 48: 2148-2153 (2010) https://doi.org/10.1016/j.fct.2010.05.020
  16. Chao C, Liu W, Wu J, Yin M. Phytochemical profile, antioxidative and anti-inflammatory potentials of Gynura bicolor DC. J. Sci. Food Agric. 95: 1088-1093 (2014)