• Title/Summary/Keyword: Bio-formulation

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Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea

  • Lee, You-Suk;Cho, Il Je;Kim, Joo Wan;Lee, Sun-Kyoung;Ku, Sae Kwang;Lee, Hae-Jeung
    • Nutrition Research and Practice
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    • v.12 no.6
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    • pp.486-493
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    • 2018
  • BACKGROUND/OBJECTIVE: The honeysuckle berry (HB) contains ascorbic acid and phenolic components, especially anthocyanins, flavonoids, and low-molecular-weight phenolic acids. In order to examine the potential of HB as a hepatoprotective medicinal food, we evaluated the in vitro anti-oxidant and anti-inflammatory activities of Korean HB (HBK) and Chinese HB (HBC). MATERIALS/METHODS: Antioxidant and anti-inflammatory effects of the extracts were examined in HepG2 and RAW 264.7 cells, respectively. The anti-oxidant capacity was determined by DPPH, SOD, CAT, and ARE luciferase activities. The production of nitric oxide (NO) as an inflammatory marker was also evaluated. The Nrf2-mediated mRNA levels of heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase [quinone] 1 (Nqo1), and glutamate-cysteine ligase catalytic subunit (Gclc) were measured. The concentrations of HB extracts used were 3, 10, 30, 100, and $300{\mu}g/mL$. RESULTS: The radical scavenging activity of all HB extracts increased in a concentration-dependent manner (P < 0.01 or P < 0.05). SOD (P < 0.05) and CAT (P < 0.01) activities were increased by treatment with $300{\mu}g/mL$ of each HB extract, when compared to those in the control. NO production was observed in cells pretreated with 100 or $300{\mu}g/mL$ of HBC and HBK (P < 0.01). Treatment with $300{\mu}g/mL$ of HBC significantly increased Nqo1 (P < 0.01) and Gclc (P < 0.05) mRNA levels compared to those in the control. Treatment with $300{\mu}g/mL$ of HBK (P < 0.05) and HBC (P < 0.01) also significantly increased the HO-1 mRNA level compared to that in the control. CONCLUSIONS: Thus, the Korean and Chinese HBs were found to possess favorable in vitro anti-oxidant and anti-inflammatory activities. Nrf2 and its related anti-oxidant genes were associated with both anti-oxidant and anti-inflammatory activities in HB-treated cells. Further studies are needed to confirm these in vivo effects.

The DeveloDment of PaDain which is Extremely Stable to Negative Ionic Environment by Directed Molecular Evolution (방향성 분자진화에 의한 음이온에 안정한 Papain 개발)

  • Kang, Whan-Koo;Hwang, Sun-Duk;Kim, Hyoung-Sik;Jeung, Jong-Sik;Lee, Bheong-Uk
    • KSBB Journal
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    • v.21 no.5
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    • pp.394-400
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    • 2006
  • When the papain, which is a sort of Cystein protease, is applied to the outer skin, it decomposes the protein which forms the peeled outer skin and speeds up metabolism. Therefore, it is one of the most important cosmetics compositic which keeps the function of skin normal. When the papain is used in cosmetics with surfactant, the activity of papain is reduced rapidly. In this study, the modified papain with extreme stability negative ionic environment was developed by directed evolution

Gel Type Formulation Utilizing Polymer-Surfactant Interaction (폴리머와 계면활성제의 상호작용을 이용한 젤타입 제형의 제조)

  • Kim, Dong-Joo;Kang, Tae-Jun;Lee, Cheon-Koo;Lee, Jung-No
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.33 no.1 s.60
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    • pp.7-10
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    • 2007
  • The interaction between polymers and surfactants was investigated by means of rheological and surface tension measurements. The polymers used in this study were acrylates/$C_{10-30}$ alkyl acrylate crosspolymer (AC) and ammonium acryloyldimethyltaurate/VP copolymer (AV). And the surfactants were PEG-40 hydrogenated castor oil (HC) and polysorbate 60 (P60). HC and P60 made the micelles intervening between AC polymers, resulting in the increase of viscosity. However, HC showed a similar behavior over the wider range of surfactant concentration than P60. Regarding of surface tensions in the same range of surfactant concentration, AC/HC solution showed the area of increasing surface tension with surfactant concentration in contrast to the AC/P60 solution showing no increasing area. It is assumed that the micelles between AC/HC were formed so cooperatively and strongly that the surfactants located at the surface originally moved to the micelles.

Quality Characteristics of Sulgidduk with Concentrated Sweet Pumpkin Powder (농축단호박 분말을 대체한 설기떡의 품질 특성)

  • Jeong, Ki-Young;Kim, Mun-Yong;Chun, Soon-Sil
    • Korean journal of food and cookery science
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    • v.24 no.6
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    • pp.849-855
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    • 2008
  • DPPH radical scavenging activities were 66.80% by hot water extract and 41.86% by 80% methanol extract. Inhibitions of angiotensin I-converting enzyme (ACE) were 91.09% by hot water extract and 75.20% by 80% methanol extract. In this study, Sulgidduk samples were prepared with 1, 3, 5, and 7% concentrated sweet pumpkin powder (CSPP), and a control were examined for quality characteristics such as moisture content, water activity, color, textural characteristics, and sensory qualities, in order to determine the optimal ratio of CSPP in the formulation. The samples containing CSPP exhibited significantly higher moisture content than the control group. Water activity was not significantly different among the Sulgidduk samples. For the color, the control group presented significantly higher lightness as compared to the samples containing CSPP. The samples containing 1 and 3% CSPP had significantly higher greenness than the samples containing 5 and 7% CSPP. Yellowness increased as the level of CSPP content increased. In terms of textural characteristics, hardness, gumminess, and chewiness were highest at the 1% substitution level, while lowest at the 3% level. The CSPP samples presented significantly higher adhesiveness, springiness, and cohesiveness than the control group. In the consumer acceptance and characteristic intensity rating test, the control group showed significantly higher color and flavor as compared to the CSPP samples. Pumpkin flavor, delicious taste, and off-flavor increased with increasing amounts of CSPP. Softness, overall acceptability, and gumminess were not significantly different among the various samples tested. Sweetness was highest at the 5% substitution level, while lowest at the 1% level. In conclusion, the results indicate that substituting $3{\sim}5%$ CSPP to Sulgidduk is optimal, providing good DPPH radical scavenging activity and inhibition of ACE as well as reasonably high overall acceptability.

Study on the Systematic Technology of Promoting Purification for the Livestock Wastewater and Reuse

  • Okada, Yoshiichi;Shim, Jae-Do;Mitarai, Masahumi;Kojima, Takayuki;Gejima, Yoshinori
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.692-700
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    • 1996
  • The objective of this study is to develop a systematic purification plant using the metabolism of aerobic microorganisms. This system is subsequently aerated and continuously removes suspended solids and settling sludges caused by aerating pressure at the bottom of a lower pipe (i.e., Continuous Removal of Suspended solids and Settling sludges, CRSS). The CRSS plants are brought out by introducing fine air bubbles into the liquid phase of a lower pipe in the bio-reactor. These plant uses aeration pipe, with multiple inlets to sweep the floor of bio-reactor tank, instead of the conventional scraper mechanisms. The principal advantage of this system is that it can continuously remove very small or light particles that settles completely within a short time. Once the particles have been floated to the surface, they can be moved into the pipe and collected in the settling tank by sequently aerated pressure. The experimental results shows that about 99.0% of the biochemical oxygen demand(BOD), 99.3% of the suspended solid(SS), 92.3% of the total nitrogen(T-N), 99.0% of the turbidity(TU), 100% of the total coliform(TC)and ammonia was respectively removed during aerobic digestion for 9 days. These result indicates that the CRS S plants are very effective for reduction and deodorization of swine wastewater contaminants, and the efflux from CRS S can either be discharged in the river or used as nutrient solution of formulation for plant growth factories. The developed CRSS plant proved to be flexible and it can simply be adapted to any type of biological waste treatment problem.roblem.

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Properties of Coating Film Containing Silicone acrylate (실리콘 아크릴레이트를 함유하고 있는 코팅필름의 물성)

  • Lee, Bong-Woo;Bang, Moon-Soo;Hong, Jin-Who;Kim, Dae-Jun;Kim, Hyun-Joong
    • Journal of Adhesion and Interface
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    • v.3 no.4
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    • pp.10-18
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    • 2002
  • Modern plastics are of great importance in many practical applications and their performance can be enhanced by surface modification to improve their hardness, wear and chemical resistance. The object of this study is invention of functional hardening coating materials which are applied to plastic, such as polycarbonate, polymethylmethacylate and acrylonitrilebutadienestyrene. In order to invention of hardening coating materials, we synthesized new silicone acrylate oligomer and prepared coating films containing silicone acrylate oligomer (SAOE) on PC substrate. The curing of coatings is performed by UV-radiation. As results of experiments, The properties of coating films which are contained SAOE is greatly improved in comparison with coating films non-contained SAOE. Especially, when 1 wt% SAOE was introduced into the coating formulation, the UV hardening coatings obtained showed the best surface hardness and gloss.

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The Effect of Consumption Value of Alternative Protein Products on Self-Efficacy and Purchase Intention

  • Choo-Yeon KIM;Gyu-Ri KIM;Seong-Soo CHA
    • The Journal of Economics, Marketing and Management
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    • v.12 no.2
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    • pp.27-36
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    • 2024
  • Purpose: As the number of vegetarians continues to rise in tandem with the development of consumer culture, a novel economic trend named 'Vegenomics' has surfaced. In addition, as interest in social and environmental sustainability such as health, environment, and animal welfare grows due to the COVID-19 pandemic, the alternative protein food market is expanding, focusing on plant-based alternative meat. Research design, data, and methodology: Therefore, this study aims to investigate the impact of the consumption value of alternative protein products on self-efficacy and purchase intention. This study collected a total of 187 questionnaires by conducting an online survey from May 1 to July 10, 2023, to verify the research model and hypothesis. The collected data were subjected to exploratory factor analysis, confirmatory factor analysis, and discriminant validity analysis using SPSS 20.0 and AMOS 20.0 programs for structural equation modeling. Results: The results of analyzing consumers' self-efficacy and purchase intention regarding the functional value, health-oriented value, ethical value, and ecological value of alternative protein products are as follows. First, among the consumption values of alternative protein products, ecological value was found to have a significant positive (+) effect on self-efficacy. Second, consumers' self-efficacy for alternative protein products was found to have a significant positive (+) effect on purchase intention. Conclusion: This study is anticipated to provide valuable insights for the formulation of effective marketing strategies for alternative protein products and the development of products that align with consumer needs.

A Study on the Effect of Pigments used in Cosmetic Manufacturing on the Form of Water Dispersible Formulations (화장품 제조에 쓰이는 Pigments 가 수분산 제형에 미치는 영향에 대한 연구)

  • Kim, Hyun Jee;Oh, Ji Won;Kwak, Byeong Mun;Lee, Mi Gi;Bin, Bum Ho
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.47 no.1
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    • pp.41-48
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    • 2021
  • Formulations which are dispersed in water are often used in color cosmetics because they are characterized by no powder flying, not sticky, and high adherence while giving a light feeling of use. However, little research has been considered on the effect of the pigment on the above formulations used in color cosmetics. In this study, experiments were conducted to measure and analyze the effect of pigments on the formulation of pigments, organic pigments, and pearl polish, which are mainly used in the manufacture of color cosmetics, on appearance changes, pH changes, and photometric stability. Carmine or ferric ferrocyanide coated titanium dioxide mica-based pigments were not suitable for use due to poor photostability, with colors appearing on the surface from low viscosity formulations. Organic pigments had a good photostability of 1% of the formulation which are dispersed in water, but were not suitable for use because they came out of color on the water surface and did not spread well due to the clumping of powders. The titanium dioxide mica system pigments coated with pearl polish, inorganic pigments, and iron oxide were suitable for use due to their excellent appearance and optical stability in the formulation. Furthermore, the pH of all samples distributed by each pigment seems to be within the range of 3.0 to 9.0, which is suitable for cosmetic applications. This is expected to help manufacture color cosmetics with a stable water dispersible formulations by selecting and using stable pigments in anticipation of the behavior of pigments in the formulations.

Reactive Dyeing of Photografted para-Aramid Fabrics

  • Kim, Eun-Min;Jang, Jin-Ho
    • Textile Coloration and Finishing
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    • v.23 no.3
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    • pp.155-162
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    • 2011
  • para-Aramid has limited dyeability because of its highly crystalline structure and compactness. To improve the dyeability of the para-aramid to reactive dyes of bright color in deep shade, the fabrics were photografted under continuous UV irradiation with dimethylaminopropyl methacrylamide and 4-benzoyl benzoic acid as a monomer and a hydrogen -abstractable photoinitiator respectively. A UV energy of 35J/$cm^2$ and a photoinitiator concentration of ten percent or more with respect to the monomer in the formulation was required for optimal photografting. Grafting yield increased with higher monomer application level. Surface analysis indicated significant alterations in the atomic composition of the photografted fabric surface and the fabric surface was covered with the grafted polymers. While the pristine para-aramid fabrics showed no appreciable dyeability to the ${\alpha}$-bromoacrylamide reactive dyes, the grafted para-aramid fabrics enhanced the dyeability to the reactive dyes substantially. In case of C.I. Reactive Blue 50, a K/S value of 8.7 can be obtained with the grafted para-aramid fabrics with a grafting yield of 2.3 %. Also the color fastness properties of the dyed fabrics was excellent in the conditions of washing, rubbing and light irradiation.

Concentration Distribution of PBDEs and PCBs in Soil (토양 중 PBDEs와 PCBs의 농도분포)

  • Lee, Sung-Hee;Cho, Ki-Chul;Yeo, Hyun-Gu
    • Journal of Environmental Health Sciences
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    • v.35 no.6
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    • pp.510-516
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    • 2009
  • Polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) were measured in soil samples of Ansung in Kyonggi-province to investigate concentration distribution of PBDEs and PCBs. The 10 soil samples were collected using a stainless steel hand-held corer that was cleaned before and after each sample using hexane. Total concentration of PBDE and PCBs were 2,205.3 and 348.1 pg/g dry weight (DW) in soil sample, respectively. BDE-209 showed as the most abundant congener in soil samples which was related to imported amount and usage amount of deca-BDE technical mixture in Korea. Also, BDE-99, BDE-47, BDE-100 deposition in soil sample was higher than other congeners and was related to the imported and usage amount reported for penta-BDE technical mixture in Korea. Correlation coefficient between PBDE contribution and technical mixture formulation (Bromokal 70-5DE) were significant (r=0.91, p<0.01) which suggests the influence of sources in this technical mixtures.