• Journal of the Korean Wood Science and Technology
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• 제35권4호
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• pp.38-44
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• 2007

환경문제의 대두에 따라 포름알데히드를 방산하는 접착제를 대신할 수 있도록 로진을 이용한 천연접착제를 개발하고자 한다. 경화속도와 접착물성을 향상시키기 위해 건조제의 배합에 따라 천연접착제를 제조하였다. 건조제의 배합에 따른 경화속도와 접착물성을 Texture Analyzer를 이용하여 얻어진 tack값과 single lap shear test를 통해 얻어진 접착력을 기반으로 평가하였으며, 외국에서 사용되고 있는 AURO, BioFa, Livos의 식물계 천연접착제와의 성능을 비교 분석하였다. 최적의 배합비는 Co 0.7 part, Zr 1.0 part, Ca 0.5 part, Activ8 0.1 part를 첨가하였을 때이며, 이 때의 접착력은 $93.2{\pm}19.1N/cm^2$의 수준에 도달하였다.

• 대한화장품학회지
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• 제42권2호
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• pp.135-143
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• 2016

본 연구에서는 파이토케미컬 펩타이드 유도체를 포함하는 니오좀 및 리포좀 제형의 안정성을 조사하였다. 여러 가지 조건에서 레시틴 혹은 계면활성제를 이용하여 제조된 리포좀 중에서 sodium palmitoyl sarcosinate와 마카다미아넛 오일을 이용한 니오좀의 안정성이 제일 우수하였다. 파이토케미컬 펩타이드 함유 니오좀(N9)의 형태는 TEM으로 확인하였으며, 입자크기는 95.7 nm로 관찰되었다. N9의 안정성은 Turbiscan 결과 ($0^{\circ}$와 $45^{\circ}$)와 제타포텐셜(-78.19 mV)로 확인하였다. N9의 펩타이드 봉입률은 BCA assay 결과 65.2%로 관찰되었다.

• 한국미생물·생명공학회지
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• 제49권2호
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• pp.157-166
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• 2021

The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated "Zeta-bio", was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

• KSBB Journal
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• 제22권1호
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• pp.7-15
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• 2007

진균류 추출물로부터 신규 멜라닌 분해효소의 분리 및 분해능 test는 멜라닌 분해효소의 균사체 배양 상등액으로 분비 생산됨을 분해능 테스트를 통하여 확인하였고, 이 분해 효소에 의한 멜라닌 분해반응은 pH 7에서 가장 높고, 30$^{\circ}C$에서 가장 안정함을 확인하였다. 멜라닌 분해 효소의 characterization은 2D-gel을 이용하여 멜라닌 분해효소 중 한 가지 효소의 pI값이 약 6.5이고 Molecular weight는 약 54-57 kDa임을 알 수 있었고, 정제된 멜라닌 분해 효소의 전체적인 단백질 및 유전자 서열을 분석하여 degenerate primer를 합성하였으며, RT-PCR 법을 이용하여 유전자를 확보하였다. 멜라닌 분해효소 생산을 위한 진균류의 배양방법 확립은 멜라닌 분해효소가 최대 활성을 갖는 배지 조건은 ammonium tartrate 0.4% : glucose 2% 배지에 yeast extract를 0.1%를 첨가한 배지임을 확인하였고, 진군류의 최적 배양온도는 24-26$^{\circ}C$였으며, 이 때 건조 균체 중량으로 약 6 g/L의 균체를 얻을 수 있었다. 또한 진균류 성장의 최적 pH는 약 5.5로서 이 경우 건조균체중량이 약 6.6 g/L였다. 정제공정은 최적화를 통하여 멜라닌 분해효소의 정제 순도 90% 이상의 정제공정을 확립하였다. Scale-up은 5 L fermenter를 이용한 기초 배양공정을 확립하여 500 L fermenter로의 경제성 있는 scale up에 성공하였고, 이 때 건조균체중량 약 14.5 g/L의 진균류를 얻었으며, 배지 중에 분비 생산된 melanin 분해효소의 양이 300 mg/L에 달하였다. 멜라닌 분해효소의 formulation은 멜라닌 분해효소를 효율적으로 피부로 전달시키기 위하여 50-100 nm 크기로 encapsulation을 실시하여 70 nm, 100 nm size의 nano capsule을 얻었다. 본 연구는 tyrosinase 저해제가 갖는 부작용이 없어 생체 친화적 물질에 의한 부작용 감소 효과가 기대되며, 독자적 기술에 의한 고부가 미백용 화장품 원료 확보로 새로운 기능에 의한 신규시장 창출이 가능하여 미백용 기능성 화장품 원료로서 고부가 화장품에 사용되어 수입대체효과 및 기업의 매출증대 효과가 있을 것으로 기대되어진다.

• The Plant Pathology Journal
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• 제34권3호
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• pp.241-249
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• 2018

Commercial biocontrol of microbial plant diseases and plant pests, such as nematodes, requires field-effective formulations. The isolate Pseudomonas chlororaphis O6 is a Gram-negative bacterium that controls microbial plant pathogens both directly and indirectly. This bacterium also has nematocidal activity. In this study, we report on the efficacy of a wettable powder-type formulation of P. chlororaphis O6. Culturable bacteria in the formulated product were retained at above $1{\times}10^8$ colony forming units/g after storage of the powder at $25^{\circ}C$ for six months. Foliar application of the diluted formulated product controlled leaf blight and gray mold in tomato. The product also displayed preventative and curative controls for root-knot nematode (Meloidogyne spp.) in tomato. Under laboratory conditions and for commercially grown melon, the control was at levels comparable to that of a standard commercial chemical nematicide. The results indicated that the wettable powder formulation product of P. chlororaphis O6 can be used for control of plant microbial pathogens and root-knot nematodes.

• Journal of Microbiology and Biotechnology
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• 제32권5호
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• pp.582-593
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• 2022

Among abiotic stresses in plants, drought and chilling stresses reduce the supply of moisture to plant tissues, inhibit photosynthesis, and severely reduce plant growth and yield. Thus, the application of water stress-tolerant agents can be a useful strategy to maintain plant growth under abiotic stresses. This study assessed the effect of exogenous bio-based 2,3-butanediol (BDO) application on drought and chilling response in tomato and turfgrass, and expression levels of several plant signaling pathway-related gene transcripts. Bio-based 2,3-BDOs were formulated to levo-2,3-BDO 0.9% soluble concentrate (levo 0.9% SL) and meso-2,3-BDO 9% SL (meso 9% SL). Under drought and chilling stress conditions, the application of levo 0.9% SL in creeping bentgrass and meso 9% SL in tomato plants significantly reduced the deleterious effects of abiotic stresses. Interestingly, pretreatment with levo-2,3-BDO in creeping bentgrass and meso-2,3-BDO in tomato plants enhanced JA and SA signaling pathway-related gene transcript expression levels in different ways. In addition, all tomato plants treated with acibenzolar-S-methyl (as a positive control) withered completely under chilling stress, whereas 2,3-BDO-treated tomato plants exhibited excellent cold tolerance. According to our findings, bio-based 2,3-BDO isomers as sustainable water stress-tolerant agents, levo- and meso-2,3-BDOs, could enhance tolerance to drought and/or chilling stresses in various plants through somewhat different molecular activities without any side effects.

• 한국식품조리과학회지
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• 제26권5호
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• pp.545-551
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• 2010

In this study, Sulgidduk samples were prepared with 0%, 3%, 5% and 7% yacon powder. Sulgidduk samples were then examined for quality characteristics such as moisture content, general components, color, texture profile analysis, sensory qualities, and sugar content, in order to determine the optimal ratio of yacon powder in the formulation. Regarding the general components of yacon Sulgidduk, the contents of crude protein and lipid decreased as the ratio of yacon powder increased. The L-value for color decreased, whereas a- and b-values increased. In the texture profile analysis, 7%-added yacon showed significantly lower hardness, whereas 7% yacon powder showed the highest values for cohesiveness, springiness, and chewiness. The contents of sucrose, fructose, and glucose in yacon Sulgidduk were found to be 3%, 5%, and 7% by LC, respectively. Sucrose showed a decreasing trend with increased yacon powder content, but fructose and glucose increased with increased amount of yacon powder. For sensory qualities, taste/flavor, texture, hardness, and overall acceptability were not significantly different among the 0%, 3%, and 5% yacon powder samples.

• 한국식품영양학회지
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• 제25권1호
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• pp.77-82
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• 2012

This study was carried out to optimize the production of immature green cherry tomato pickles and to produce green cherry tomato pickles of good sensory quality. The composition of immature green cherry tomato pickles was optimized using a central composition design with 3 variables and 3 levels. The overall acceptability score, based on sensory evaluation, was best, when the immature green cherry tomato pickles contained 231 g of vinegar, 52.6 g of salt, 168.3 g of sugar, 204 g of tomatoes, and 231 $m{\ell}$ of water. The statically predicted optimal formulation of immature green cherry tomato pickles on overall acceptability value was 33.54%(w/w) of vinegar, 7.64%(w/w) of salt, 25.28%(w/w) of sucrose, 33.54%(w/w) of water. The optimal conditions for producing immature green cherry tomato pickles should consider the factors of time and temperature of storage.

• 한국염색가공학회지
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• 제23권1호
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• pp.11-20
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• 2011

UV-induced surface copolymerization has been widely applied as a simple, useful and versatile approach to improve the surface properties of textiles. C.I. Reactive Black 5 and acrylic acid (AA) were continuously grafted onto cotton by UV irradiation. The photografting may occur by the copolymerization of AA with the vinylsulfone reactive dye which photochemically converted from the bissulfatoethylsulfone reactive group. The graft yield and color yield were influenced by UV energy, the dye and photoinitiator concentrations, a mole ratio of AA to dye, and pH. The coloration of cotton fabrics having a K/S of 7.0 can be obtained under a UV irradiation energy of 15$J/cm^2$ by the photografting of an aqueous alkaline formulation of 6% dye concentration containing 3% photoinitiator concentration on the weight of monomers, and a 3:1 mole ratio addition of AA to the dye. Furthermore, the photochemically dyed cotton fabrics showed comparable washing (staining) and rubbing fastness to conventional reactive dyeing method except shade change in the wash fastness and light fastness.

• Applied Biological Chemistry
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• 제47권3호
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• pp.332-338
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• 2004

농약 원제, 왕겨, 무기산화제 및 아교 혼합 분말에 물을 가하여 반죽한 다음 성형하여 봉상 훈연제를 제제하고 훈연제의 연소성을 측정하여 보편적으로 사용될 수 있는 산화제를 선발하고자 하였다. 왕겨 분말을 가연성 담체로 하는 봉상 훈연제는 11개의 농약에 대하여 빠르고 균일한 연소 속도와 높은 훈연율을 나타내었다. 연소 균일성, 연소 속도 및 훈연율을 고려할 때 봉상 훈연제에는 산화제로써 염소산나트륨이 가장 적당하였다. 가장 높은 훈연율을 나타내는 산화제 첨가비는 농약에 따라 다르게 나타났지만 연소 속도가 다른 훈연제가 훈연율도 높게 나타나는 경향이었다. 왕겨를 가연성 담체로 한 훈연제는 $50^{\circ}C$에서 60일간 보관하였을 때 대부분 유효성분의 경시변화가 거의 없이 안정하였다. 연소실, 냉각기, 포집병 1, 여과기, 포집병 2, 포집병 3, 포집병 4, 응축기 및 진공펌프로 구성된 연기 포집 장치는 연소시 연기가 대량으로 발생하는 훈연제의 훈연율 측정에 적합하였다.