• Title/Summary/Keyword: Bio-conversion

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A comparison study of extraction methods for bio-liquid via hydrothermal carbonization of food waste

  • Bang, YeJin;Choi, Minseon;Bae, Sunyoung
    • Analytical Science and Technology
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    • v.31 no.3
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    • pp.112-121
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    • 2018
  • The hydrothermal carbonization method has received great attention because of the conversion process from biomass. The reaction produces various products in hydrochar, bio-liquid, and gas. Even though its yield cannot be ignored in amount, it is difficult to find research papers on bio-liquid generated from the hydrothermal carbonization reaction of biomass. In particular, the heterogeneity of feedstock composition may make the characterization of bio-liquid different and difficult. In this study, bio-liquid from the hydrothermal carbonization reaction of food wastes at $230^{\circ}C$ for 4 h was investigated. Among various products, fatty acid methyl esters were analyzed using two different extraction methods: liquid-liquid extraction and column chromatography. Different elutions with various solvents enabled us to categorize the various components. The eluents and fractions obtained from two different extraction methods were analyzed by gas chromatography with a mass spectrometer (GC/MS). The composition of the bio-liquid in each fraction was characterized, and seven fatty acid methyl esters were identified using the library installed in GC/MS device.

Java DOM Parsers to Convert KGML into SBML and BioPAX Common Exchange Formats

  • Lee, Kyung-Eun;Jang, Myung-Ha;Rhie, A-Rang;Thong, Chin Ting;Yang, San-Duk;Park, Hyun-Seok
    • Genomics & Informatics
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    • v.8 no.2
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    • pp.94-96
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    • 2010
  • Integrating various pathway data collections to create new biological knowledge is a challenge, for which novel computational tools play a key role. For this purpose, we developed the Java-based conversion modules KGML2SBML and KGML2BioPAX to translate KGML (KEGG Markup Language) into a couple of common data exchange formats: SBML (Systems Biology Markup Language) and BioPAX (Biological Pathway Exchange). We hope that our work will be beneficial for other Java developers when they extend their bioinformatics system into SBML- or BioPAX-aware analysis tools. This is part of our ongoing effort to develop an ultimate KEGG-based pathway enrichment analysis system.

Improved Ectoine Production from Methane by Optimization of the Bio-milking Process in Engineered Methylomicrobium alcaliphilum 20Z

  • Lee, Yun Seo;Chai, Hanyu;Cho, Sukhyeong;Na, Jeong Geol;Lee, Jinwon
    • Korean Chemical Engineering Research
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    • v.60 no.3
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    • pp.392-397
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    • 2022
  • Methane is one of the major greenhouse gases, recently, the biotechnological conversion from methane to high-value added chemicals have emerged as an effort to reduce methane gas emission. In this study, we optimized ectoine bio-milking conditions in which cells were repeatedly used to improve intracellular and extracellular ectoine yield from methane by using Methylomicrobium alcaliphilum 20ZDP2. First, the cultivation and intracellular ectoine accumulation conditions were optimized with respect to the growth phase and medium salinity to achieve the highest yield of synthesis. Second, ectoine excretion was optimized by determining the ectoine secretion time (15 min) in appropriate medium salinity under hypoosmotic conditions (1% NaCl). Finally, bio-milking of ectoine was successfully repeated more than 10 times using M. alcaliphilum 20ZDP2, and the ectoine yield was improved up to 129.29 mg/ DCW g.

Conversion of Brown Materials, Crude Lipids, Crude Proteins and Aromatic Compounds of Changed Ginseng by 9 Repetitive Steaming and Drying Process (인삼의 구증구폭(九蒸九曝)에 의한 갈변물질, 조지방, 조단백 및 향기성분의 변화)

  • Kim, Do-Wan;Lee, Yun-Jin;Min, Jin-Woo;Lee, Bum-Soo;In, Jun-Gyo;Yang, Deok-Chun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.2
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    • pp.333-339
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    • 2008
  • Korean ginseng (Panax ginseng C. A. Meyer) has been used as an important medicinal plant in the Orient for a long time. It has been claimed that ginseng has many beneficial bioactive effects on human health, such as antitumor, antistress, antiaging and enhancing immune functions. Red ginseng possibly have new ingredients converted during steaming and dry process from fresh ginseng. Kujeungkupo method which means 9 repetitive steaming and drying process was used for the processes of green tea, Polygonatum odoratum, and Rehmanniae radix preparata. In this study, ingredient conversion of ginseng by 9 repetitive steaming and drying process were investigated measuring conversion efficiency of brown materials, crude lipids, crude proteins and aromatic compounds. Brown materials, as an antioxidant, in red ginseng were produced through non-enzymatic reaction by heat. Repetitive steaming and drying treatments on ginseng root contiunously increased the content of brown materials and the chromaticity. Crude lipids were degraded by heat and converted into volatile aromatic ingredients. Crude lipids were degraded and decreased by 0.52% after the 5th and 7th. Crude proteins were also decomposed and converted to amino acid. Crude proteins after the 9th treatment were decreased by more than 85% as increased times of treatments. A bicyclogermacrene as aromatic material was decreased as increased treatment times, while but a aromatic caramel was increased.

Bio-Denitrification of the Nitrate Waste Solution from the Lagoon Sludge in a Batch Fermenter (회분식 발효조에서 미생물을 이용한 라군 슬러지 질산염 폐액의 탈질 공정 평가)

  • Oh Jong-Hyeok;Lee O-Mi;Hwang Doo-Seong;Choi Yun-Dong;Hwang Sung-Tae;Jo Byung-Real;Park Jin-Ho
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.4 no.2
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    • pp.153-159
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    • 2006
  • It is a serious task to the decommissioning of the uranium conversion plant that the demolition of the lagoon sludge. The main component of the sludge is ammonium nitrate and that is the very explosive material. Therefore, the bio-denitrification is a attractive process to remove the nitrate. In this work, some process variables was tested such as incubation temperature, nitrate concentration, electron donor, C/N ratio, seeding ratio, and pH with an anaerobic bacteria as Pseudomonas halodenitrificans. The results would be used as basic data to the continuous bio-denitrification process.

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Synthesis of Red Light Emitting Au Nanocluster (적색 발광하는 금 나노클러스터 합성)

  • Cha, Dae Kyeong;Yoon, Sang Min;Kim, Mi Sung;Bang, Ji Won
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.29 no.11
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    • pp.685-689
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    • 2016
  • Synthesis of the fluorescent Au nanoclusters is reported. The Au nanoclusters were synthesized via reduction of gold ions in reverse micelles with mild reducing agents. The Au nanoclusters show a bright red emission at 640 nm. The fluorescent Au nanoclusters attract great interest for sensor, electronic device and bio-imaging applications because of ultra-small size, high chemical stablity and bright emission. We believe that the fluorescent Au nanoclusters can have optoelectronic applications such as optical down conversion phosphors.

Review on the East Pyrolysis of Biomass and Characteristics of Bio-Oil (바이오매스의 Fast Pyrolysis 공정과 Bio-Oil의 특성)

  • Myung So-Yuung;Park Young-Kwon;Jeon Jong-Ki;Kim Joo-Sik
    • Resources Recycling
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    • v.13 no.1
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    • pp.3-13
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    • 2004
  • The use of biomass has attracted extensive attention from the beginning of civilization. However, intensive researches on the biomass from the view point of the development of alternative energy have been carried out just recently. Fast pyrolysis, as a tool for the utilization of the biomass as the secondary energy source has drawn great attentions due to high applicability for the production of several valuable materials from biomass. This review paper focuses on the recent developments of pyrolysis process and reports the characteristics of bio-oil, which is the main product of fast pyrolysis of biomass.

High Correlation between Alu Elements and the Conversion of 3' UTR of mRNAs Processed Pseudogenes

  • An, Hyeong Jun;Na, Dokyun;Lee, Doheon;Lee, Kwang Hyung;Bhak, Jonghwa
    • Genomics & Informatics
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    • v.2 no.2
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    • pp.86-91
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    • 2004
  • Even though it represents $6-13\%$ of human genomic DNA, Alu sequences are rarely found in coding regions. When in exon region, over $80\%$ of them are found in 3' untranslated region (UTR). Pseudogenes are an important component of human genome. Their functions are not clearly known and the mechanism of how they are generated is still debatable. Both the Alu and Pseudogenes are important research problems in molecular biology. mRNA is thought to be a prime source of pseudogene and active research is going on its molecular mechanism. We report, for the first time, that mRNAs containing Alu repeats at 3' UTR has a significantly high correlation with processed pseudogenes, suggesting a possibility that Alu containing mRNAs have a high tendency to become processed pseudogenes. It is known that about $10\%$ of all human genes have been transposed. Transposed genes at 3' UTR without Alu repeat have about two processed pseudogenes per gene on average while we found with statistical significance that a transposed gene with Alu had over three processed Pseudogenes on average. Therefore, we propose Alu repeats as a new and important factor in the generation of pseudogenes.