• Asian-Australasian Journal of Animal Sciences
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• v.28 no.9
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• pp.1303-1308
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• 2015

This study was conducted to investigate changes in blood enzyme parameters and to evaluate the relationship between insulin-like growth factor-1 (IGF-1), antler growth and body weight during the antler growth of sika deer (Cervus nippon). Serum enzyme activity and IGF-1 concentrations were measured in blood samples collected from the jugular and femoral veins at regular intervals during the antler growth period. Blood samples were taken in the morning from fasted stags (n = 12) which were healthy and showed no clinical signs of disease. Alfalfa was available ad libitum and concentrates were given at 1% of body weight to all stags. The experimental diet was provided at 9 am with water available at all times. There were no significant differences in alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase during antler growth, but alkaline phosphatase concentrations increased with antler growth progression, and the highest alkaline phosphatase concentration was obtained 55 days after antler casting. Serum IGF-1 concentrations measured from blood samples taken from the jugular vein during antler growth, determined that levels of IGF-1 was associated with body weight and antler growth patterns. Serum IGF-1 concentrations were higher at the antler cutting date than other sampling dates. Antler length increased significantly during antler growth (p<0.001), and there was a similar trend to between right and left beams. Body weight increased with antler growth but was not significant. Consequently it appeared that serum alkaline phosphatase concentration was related to antler growth and both antler growth and body weight were associated positively with IGF-1 concentrations during antler growth.

• Journal of Environmental Impact Assessment
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• v.27 no.1
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• pp.83-91
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• 2018

Media (bio-stone), aquatic macrophytes (Oenanthe javanica) and herbivorous cladoceran (Daphnia similoides) have been used in artificial floating island (AFI) systems for water pollution control. Efficiency in chl-a concentration controlling of AFI was tested using different combinations of each device: G-BD-mixture bio-stone and Daphnia similoides, G-OB-mixture Oenanthe javanica and bio-stone, G-BOD-mixture bio-stone, Oenanthe javanica and Daphnia similoides, and the out-put water quality improvement was compared with G-C-control (no device was applied). We analyzed removal efficiency of chl-a concentration and nutrient concentrations in the artificially eutrophic water in the laboratory experimental facility. The results showed average removal rates of Chlorophyll a, TN and TP for different four groups: 69.24%, 16.61%, -0.61%; 68.39%, 14.11%, 10.52%; 78.30%, 6.69%, 25.09%; 35.42%, -3.47%, -25.18%, respectively. The results have suggested that the mixture of media, plants and zooplankton is the most efficient combination for Chlorophyll a control, while the mixture of macrophytes and bio-stone have better efficiency nutrient control.

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1083-1095
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• 2019

Butyrate is known to play a significant role in energy metabolism and regulating genomic activities that influence rumen nutrition utilization and function. Thus, this study investigated the effects of an isolated butyrate-producing bacteria, Clostridium saccharobutylicum, in rumen butyrate production, fermentation parameters and microbial population in Holstein-Friesian cow. An isolated butyrate-producing bacterium from the ruminal fluid of a Holstein-Friesian cow was identified and characterized as Clostridium saccharobutylicum RNAL841125 using 16S rRNA gene sequencing and phylogenetic analyses. The bacterium was evaluated on its effects as supplement on in vitro rumen fermentation and microbial population. Supplementation with $10^6CFU/ml$ Clostridium saccharobutylicum increased (p < 0.05) microbial crude protein, butyrate and total volatile fatty acids concentration but had no significant effect on $NH_3-N$ at 24 h incubation. Butyrate and total VFA concentrations were higher (p < 0.05) in supplementation with $10^6CFU/ml$ Clostridium saccharobutylicum compared with control, with no differences observed for total gas production, $NH_3-N$ and propionate concentration. However, as the inclusion rate (CFU/ml) of C. saccharobutylicum was increased, reduction of rumen fermentation values was observed. Furthermore, butyrate-producing bacteria and Fibrobacter succinogenes population in the rumen increased in response with supplementation of C. saccharobutylicum, while no differences in the population in total bacteria, protozoa and fungi were observed among treatments. Overall, our study suggests that supplementation with $10^6CFU/ml$ C. saccharobutylicum has the potential to improve ruminal fermentation through increased concentrations of butyrate and total volatile fatty acid, and enhanced population of butyrate-producing bacteria and cellulolytic bacteria F. succinogenes.

• Analytical Science and Technology
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• v.21 no.1
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• pp.34-40
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• 2008

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of lercanidipine in human plasma. After addition of internal standard (amlodipine), plasma was precipitated with acetonitrile and the supernatant was evaporated. The residues were dissolved in 50 % acetonitrile and analyzed by LC-MS/MS. Using MS/MS with multiple reaction monitoring(MRM) mode, lercanindipine were selectively detected without severe interference from human plasma. The standard calibration curve for lercanidipine was linear (r = 0.9994) over the concentration range 0.05-20.0 ng/mL in human plasma. The intra- and inter-day precision over the concentration range of lercanidipine was lower than 11.7 % (correlation of variance, CV), and accuracy was between 94.4-114.8 %. This method has been successfully applied to the pharmacokinetic study of lercanidipine in human plasma.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.1540-1545
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• 2004

Measurement of concentration fields in a micro-channel is the crucial technology in the area of Lab-on-a-chip to be used for various bio-chemical applications. It is wel-known that the only possible way to measure the concentration field in the micro-channel is using micro-LIF(Laser Induced Fluorescence) method. However, an accurate concentration field at a given cross plane in a micro-channel has not been made so far due to the limit of light illumination. The present study demonstrates a novel method to provide an ultra thin laser sheet beam having 5 microns thickness by a micro focus laser line generator. Nile Blue A was used as fluorescent dye for LIF measurement. The laser sheet beam illuminates an exact plane of concentration measurement in the micro-channel to increase the signal to noise ratio and reduce the depth uncertainty considerably.

• Journal of Korean Society of Water and Wastewater
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• v.12 no.4
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• pp.59-69
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• 1998

Biological treatment systems generally can be divided into two main classes of a suspended sludge process and attached one like a fluidized bed reactor. These process are considered to bring remarkable change in species composition of microorganisms, due to difference of a state of biofilm, a concentration and diffusion velocity of dissolved oxygen, a concentration and diffusion velocity of substance or poisonous matter. The change of species composition bring different treatment result for influence factors like F/M ratio, DO concentration, pH or poisonous matter. This study is to investigate the reaction characteristics of both microorganisms, namely, a suspended sludge and attached sludge, through the changes of pH, temperature and substance concentration.

• Korean Journal of Remote Sensing
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• v.16 no.4
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• pp.315-325
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• 2000

Observations were made to validate ocean color algorithms in the Ulleung Basin, East Sea in May 2000. Small scale and meso-scale surveys were conducted for the validation of ocean color products (nLw: normalized water-leaving radiance and chlorophyll concentration). There were discrepancies between SeaWiFS and in situ nLw showing the current aerosol models of standard SeaWiFS processing software are less than adequate (Gordon and Wang, 1994). Applying the standard SeaWiFS in-water algorithm resulted in an overestimation of chlorophyll concentration. This is because that CDOM absorption was higher than the estimated chlorophyll absorption. TSS concentration was also high. Therefore, the study region deviated from Case 1 waters. The source of these materials seems to be the entrainment of coastal water by the Tsushima Warm Current. Study of the bio-optical properties in other season is desirable.

• Development and Reproduction
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• v.15 no.4
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• pp.301-308
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• 2011

The tolerance evaluation for abalone Haliotis discus hannai embryos was performed using different concentrations of cryoprotective agents (CPAs): dimethyl sulfoxide, ethylene glycol and propylene glycol added to 0.2 M sucrose, respectively. 4-cell, trochophore and veliger were exposed in each CPA with different concentration for 10, 20 and 30 minutes of immersion time. Developmental rates were increased with decreased concentration of every CPA and decreased immersion time, and differed from types of CPA. Developmental rates of veliger in all the CPAs were higher than those of 4-cell and trochophore. The developmental rates and larval activity indices in ethylene glycol were comparatively higher than those in other CPAs and the effective CPA and its concentration for the cryopreservation of the abalone embryos was suggested as 2.0 M ethylene glycol with equilibration time of 30 minutes.

• Journal of Bio-Environment Control
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• v.5 no.2
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• pp.138-144
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• 1996

This experiment was conducted to decide appropriate nutrient concentration for beet soilless culture by different nutrient concentration treatment between before and after root enlargement. Yamazaki's solution for head lettuce was used 0.5, 1, 2, 4 strength for before root enlargement and 0.5, 1, 2, 4 strength was treated after root enlargement. Root weight was good in 0.5, 1, 2 strength treatment before, then change treatment to 4 strength after root enlargement. But 4 strength treatment after root enlargement increase pithiness and root cracking. Vitamin C contents was low in 0.5 and 4 strength treatments after root enlargement. Betaxanthin and betacyanin were involved high concentration in lower nutrient concentration after root enlargement despite the treatments of before root enlargement. Therefore 1, 2 strength before root enlargement and 2 strength after root enlargement treatments is recommendable for nutrient culture of beet.

• Journal of Applied Biological Chemistry
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• v.59 no.1
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• pp.37-43
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• 2016

Bioactive components of ultra-fine ground Saururus, the extraction yield increases when the leaves are ultra-fine ground. Comparison of normal-ground and ultra-fine ground Saururus chinensis leaves showed that the solid content and antiinflammatory activity of ultra-fine ground extracts was higher than that of normal-ground extracts. Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells were treated with different concentrations of Saururus chinensis extract and the amount of nitric oxide (NO) was determined; LPS-treated cells produced 2 times more NO than cells that were not treated with LPS. Moreover, the NO production in cells treated with Saururus chinensis extract was inhibited in a concentration-dependent manner. Because the stimulant-induced NO production is regulated by the inducible nitric oxide synthase (iNOS), we measured the iNOS protein level to elucidate the mechanism by which the NO production was inhibited. We found that the amount of iNOS decreased dose-dependently. It was reduced by 53% at a Saururus chinensis extract concentration of $100{\mu}g/mL$. The protein expression of cyclooxygenase-2 (COX-2) in LPS-treated Raw 264.7 cells was inhibited by 31% at $100{\mu}g/mL$ of Saururus chinensis extract. Gel shift of the nuclear factor kappa B-DNA complex occurred in LPS-treated cells and the intensity of the band decreased gradually in a concentration-dependent manner. Ultra-fine ground Saururus chinensis extract had a concentration-dependent inhibitory effect on the production of prostaglandin $E_2$, tumor necrosis factor ${\alpha}$, interleukin $1{\beta}$ (IL-$1{\beta}$), IL-6, and IL-8 in LPS-treated Raw 264.7 cells, i.e., at $50{\mu}g/mL$ of Saururus chinensis extract, their levels were decreased by 53, 67, 52, 37, and 21% respectively.