• Tribology and Lubricants
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• v.31 no.6
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• pp.251-257
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• 2015

Heavy oil production is receiving significant attention because of increased demands for thermal power generation systems of the diesel engine and boilers. However, asphaltene, which is a heavy oil components (6-8 wt%), reduces the heat efficiency of the fuels owing to its agglomerated sludge of asphaltene during the burning process. Therefore, for hassle-free operation, we should develop asphaltene dispersants to suppress the formation of the sludge. We prepare variable salt-type polymeric dispersants using poly(isobutenyl succinic anhydride) and poly(amine) through both condensation esterification and acid-base neutralization reactions, which we subsequently evaluate for dispersing performance, using Turbiscan measurement. Total acid number (TAN) and total base number (TBN) of 75Lec-25SynDis.2 composed of lecithin and the prepared polymeric salt having the ratio of 3 : 1 are 18.9 and 33.7 mg KOH/g, respectively, which are comparable to those of the commercial dispersants (15.8 and 26.5 mg KOH/g). We determine the initial turbidity observed for 15 min of the polymeric dispersant was determined with transmittance (%), which can be calculated to separability number (SN). The SN value of 75Lec-25SynDis.2 is close to zero, which is superior to that of commercial dispersants and lecithin (0.015 and 0.017).

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.435-438
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• 2000

Crude oil-degrading microorganisms, Acinetobacter sp. A132, Pseudomonas aeruginosa F722, and Acinetobacter calcoaceticus OM1 were isolated from soil and sea. The optimal temperature of strain A132 and strain F722 on growth isolated from soil was $35^{\circ}C$ both, and also their growth were optimized at pH 8 and 9, respectively. The growth of the strains, A132 and F722, showed that crude oil of 2% (w/v) in culture broth in which crude oil was used as carbon and energy sources appeared to be an optimum. Optimal culture conditions of strain OM1 were different from those of the soil microorganisms except for temperature. The growth of strain OM1 was optimized at pH 7 and crude oil of 3.0% (w/v). The degradability to crude oil by strain A132 showed maximum $5.49g/\;l\;{\cdot}\;day$ under the conditions of $25^{\circ}C$, NaCl of 1.0% (w/v), and crude oil of 2.0% (w/v). The highest degradability of strain F722 to crude oil was $1.19g/\;l\;{\cdot}\;day$ under the culture conditions at $35^{\circ}C$, NaCl 1.0% (w/v), and crude oil of 2.0% (w/v). The degradation characteristics of kerosene $(nC_9-nC_{20})$ and diesel $(nC_9-nC_{28})$ by strain OM1, and F722 were analyzed by gas chromatography. Strain OM1 degraded more than 95% of kerosene and 75% of diesel for 7 days cultivation. Strain F722 showed degradation of more than 80% to kerosene in 10 days.

• Journal of Life Science
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• v.21 no.11
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• pp.1599-1606
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• 2011

To evaluate the effects of microorganism agents on oil biodegradation, treatability and microcosm studies were conducted. Petroleum oil degrading bacteria were isolated from enriched cultures of oil-contaminated sediment samples using a mineral salts medium (MSM) containing 0.5% Arabian heavy crude oil as the sole carbon source. After a 5 day-incubation period using MSM, mixed microorganisms of three species (strains BS1, BS2 and BS4) degraded 48.4% of aliphatic hydrocarbons and 30.5% of aromatic hydrocarbons. Treatability and microcosm tests were performed in the three different treatment conditions (AO: Arabian heavy crude oil, AO+IN: Arabian heavy crude oil+inorganic nutrient, AO+IN+MM: Arabian heavy crude oil+inorganic nutrient+mixed microorganism agents). Among these, significantly enhanced biodegradation of aliphatic hydrocarbons were observed in AO+IN and AO+IN+MM conditions, without showing any different biodegradation rates in either condition. However, the degradation rates of aromatic hydrocarbons in an AO+IN+MM condition were increased by 50% in the treatability test and by 13% in the microcosm test compared to those in an AO+IN condition. Taken together, it can be concluded that mixed microorganism agents enhance the biodegradation of aliphatic and aromatic hydrocarbons in laboratory, a treatability test, and a microcosm test. This agent could especially be a useful tool in the application of bioremediation for removal of aromatic hydrocarbons.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.354-361
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• 2009

This study was carried out to develop an effective biocarrier-mediated bioaugmentation technology which will be useful for remediation of the crude oil-contaminated marine sediments. Enrichment of several microbial communities was made from several oil-polluted seashore sites and the two distinctively functional consortia have been successfully selected. These two consortia were grown together and used to manufacture the microbial agents for bioaugmentation of marine sediments polluted with crude oil. The most dominant species in the mixed culture was identified as Alcanivorax borkumensis based on pure culture and DGGE analysis. Bioaugmentation of oil-polluted marine sediments with the microbial agent MA-2 formulated using the mixed culture and biocarriers (activated carbon and minerals) was more effective, especially in combination with an oxygen producing (releasing) compound (ORC). Ninty percent of TPH was removed in the presence of ORC in 35 days while 74% in the absence of ORC. This indicated that the indigenous consortial degraders could be immobilized on the active carbon as a biocarrier to manufacture microbial agents and then effectively bioaugmented for remediation of the oil-polluted sediments.

• Animal Bioscience
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• v.36 no.7
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• pp.1044-1058
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• 2023

Objective: The objective of this study was to characterize physiochemical and nutrient profiles of feedstock and co-products from canola bio-oil processing that were impacted by source origin. The feedstocks and co-products (mash, pellet) were randomly collected from five different bio-oil processing plants with five different batches of samples in each bio-processing plant in Canada (CA) and China (CH). Methods: The detailed chemical composition, energy profile, total digestible nutrient (TDN), protein and carbohydrate subfractions, and their degradation and digestion (CNCPS6.5) were determined. Results: The results showed that TDN_1x was similar in meals between CA and CH. CH meals and feedstock had higher, truly digestible crude protein (tdCP) and neutral detergent fiber (tdNDF) than CA while CA had higher truly digestible non-fiber carbohydrate (tdNFC). The metabolizable energy (ME_3x), net energy (NE_Lp3x, NE_m3x, and NE_g3x) were similar in meals between CA and CH. No differences were observed in energy profile of seeds between CA and CH. The protein and carbohydrate subfractions of seeds within CH were similar. The results also showed that pelleting of meals affected protein sub-fractionation of CA meals, except rapidly degradable fractions (PB1), rumen degradable (RDPB1) and undegrdable PB1 (RUPB1), and intestinal digestible PB1 (DIGPB1). Canola meals were different in the soluble (PA2) and slowly degradable fractions (PB2) between CA and CH. The carbohydrate fractions of intermediately degradable fraction (CB2), slowly degradable fraction (CB3), and undegradable fraction (CC) were different among CH meals. CH presented higher soluble carbohydrate (CA4) and lower CB2, and CC than CA meals. Conclusion: The results indicated that although the seeds were similar within and between CA and CH, either oil-extraction process or meal pelleting seemed to have generated significantly different aspects in physiochemical and nutrient profiles in the meals. Nutritionists and producers need to regularly check nutritional value of meal mash and pellets for precision feeding.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.10
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• pp.1851-1859
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• 2000

Crude oil-degrading bacteria were isolated from the sites contaminated by oil products. The isolates were identified as Acinetobacter sp. A132, Pseudomonas putida A422, Pseudomonas aeruginosa F721, F722, and Xanthomonas maltophilia B823. The results of investigation on the degradability of crude oil indicated that the strain A132 had the highest rate of $6.04g/L{\cdot}day$. Also, the strain A132 and F722 almost degraded each of n-alkane compounds between $nC_{10}$ and $nC_{32}$. The strain A422 degraded benzene and xylene but not n-alkane. The strain B823 grew somewhat in crude oil but did not entirely degrade other substrates used in this study. The results of the GC/FID analysis on the degradability of the mixed n-alkane compounds showed that the strain F722 could degrade 100% of the compounds with $nC_7{\sim}nC_{10}$ and more than 80% of those with $nC_{11}{\sim}nC_{24}$.

• Journal of the Korean Applied Science and Technology
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• v.34 no.1
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• pp.1-11
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• 2017

Bio-oil has attracted considerable interest as one of the promising renewable energy resources because it can be used as a feedstock in conventional petroleum refineries for the production of high value chemicals or next-generation hydrocarbon fuels. Zeolites have been shown to effectively promote cracking reactions during pyrolysis resulting in highly deoxygenated and hydrocarbon-rich compounds and stable pyrolysis oil products. In this study, catalytic pyrolysis was applied to upgrade bio-oil from yellow poplar and then fuel characteristics of upgraded bio-oil was investigated. Yellow Poplar(500 g) which ground 0.3~1.4 mm was processed into bio-oil by catalytic pyrolysis for 1.64 seconds at $465^{\circ}C$ with Control, Blaccoal, Whitecoal, ZeoliteY and ZSM-5. Under the catalyst conditions, bio-oil productions decreased from 54.0%(Control) to 51.4 ~ 53.5%, except 56.2%(Blackcoal). HHV(High heating value) of upgraded bio-oil was more lower than crude bio-oil while the water content increased from 37.4% to 37.4 ~ 45.2%. But the other properties were improved significantly. Under the upgrading conditions, ash and TAN(Total Acid Number) is decrease and particularly important as transportation fuel, the viscosity of bio-oil decreased from 6,933 cP(Control) to 2,578 ~ 4,627 cP. In addition, ZeoliteY was most effective on producing aromatic hydrocarbons and decreasing of from the catalytic pyrolysis.

• Applied Chemistry for Engineering
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• v.19 no.6
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• pp.592-599
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• 2008

Oil sands had not received enough attention due to high production cost. However, as oil price significantly increases, oil sands are receiving more and more interest as unconventional crude oil. The value and applicability of oil sands can be enhanced by upgrading oil sands bitumen to produce synthetic crude oil (SCO). This study analyzed 213 oil sands upgrading patents applied between 1969 and 2006 in US, Canada, Japan, Europe, and Korea. The upgrading technologies could be classified into 9 detailed technologies; hydrocracking, coking, thermal cracking, deasphalting, supercritical technology, bio-technology, hydrotreating, gasification, and others. The number of patents applied for oil sands upgrading increased after 1970, reached a maximum in the early 1980, and slowly increases again in recent years. Korea has a lack of technologies for oil sands. Therefore, the technologies for oil sands production and application, specially, upgrading technologies based on accumulated oil refinery technologies need to be developed to increase self-development ratio of energy resource.

• Korean Journal of Organic Agriculture
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• v.9 no.3
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• pp.23-44
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• 2001

To address complex solutions to tack1e the cost rise with the increasing of the prices of crude oil, and to lower warming gas discharge in agricultural sector in line with the framework Convention on Climate Change, we need to net only develop the methods of simply reducing energy use ratio, but also effectively energize resources discharged in the course of agricultural production aimed at enabling repealed farming, and develop systems to reinvest such energy into agricultural production and apply them to actual farming. To that end, specifically, we need to reduce the energy cost in agricultural production, and produce and harness bio gas that makes it possible to stabilize the continued supply of energy. Thus, in this research, we seek to discuss energy use volume and warming gas discharge, and the characteristics of bio gas and its use methods alike, in agricultural sector, and thus present methods for environment-friendly and continual development in agriculture.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.4
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• pp.390-398
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• 2014

We conducted an 8-week feeding trial to evaluate dietary lipid sources on the growth performance and body composition of juvenile river puffer fish Takifugu obscurus. Nine experimental diets were formulated with fishmeal as the major protein ingredients, providing 50% crude protein. The experimental diets contained either beef fallow (BF), soybean oil (SO), rapeseed oil (RO), or linseed oil (LO). Each of these diets was then supplemented or not with 0.5% n-3 HUFA (BFH, SOH, ROH, and LOH), resulting in a total of eight experimental diets. The control diet contained fish oil (FO) as the lipid source. Fish averaging $10.3{\pm}0.03g$ were fed the experimental diets in randomly selected triplicate groups for 8 weeks. Weight gain and feeding efficiency of fish fed the FO and SOH diets were significantly higher than those of fish fed BF or RO (P<0.05), but these diets did not differ significantly from the other diets. The protein efficiency ratio of fish fed the SOH diet was significantly higher than that of fish fed the BF, SO, or RO diets (P<0.05), but these were not significantly different from the other diets. The specific growth rate of fish fed the FO and SOH diets was significantly higher than that of fish fed the BF diet (P<0.05). Whole body DHA and n-3 HUFA contents of fish fed the FO diet were significantly higher than those of fish fed the SO, RO, or LO diets (P<0.05), but were not significantly different from the other diets. These results indicate that soybean oil and linseed oil could replace up to 100% of fish oil in the diet containing 60% fishmeal for river puffer fish.