• Title/Summary/Keyword: Bio Sample

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Development of Deep Learning AI Model and RGB Imagery Analysis Using Pre-sieved Soil (입경 분류된 토양의 RGB 영상 분석 및 딥러닝 기법을 활용한 AI 모델 개발)

  • Kim, Dongseok;Song, Jisu;Jeong, Eunji;Hwang, Hyunjung;Park, Jaesung
    • Journal of The Korean Society of Agricultural Engineers
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    • v.66 no.4
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    • pp.27-39
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    • 2024
  • Soil texture is determined by the proportions of sand, silt, and clay within the soil, which influence characteristics such as porosity, water retention capacity, electrical conductivity (EC), and pH. Traditional classification of soil texture requires significant sample preparation including oven drying to remove organic matter and moisture, a process that is both time-consuming and costly. This study aims to explore an alternative method by developing an AI model capable of predicting soil texture from images of pre-sorted soil samples using computer vision and deep learning technologies. Soil samples collected from agricultural fields were pre-processed using sieve analysis and the images of each sample were acquired in a controlled studio environment using a smartphone camera. Color distribution ratios based on RGB values of the images were analyzed using the OpenCV library in Python. A convolutional neural network (CNN) model, built on PyTorch, was enhanced using Digital Image Processing (DIP) techniques and then trained across nine distinct conditions to evaluate its robustness and accuracy. The model has achieved an accuracy of over 80% in classifying the images of pre-sorted soil samples, as validated by the components of the confusion matrix and measurements of the F1 score, demonstrating its potential to replace traditional experimental methods for soil texture classification. By utilizing an easily accessible tool, significant time and cost savings can be expected compared to traditional methods.

Distribution Characteristics of Heavy Metals in Soil in the Vicinity of King Sejong Station, King George Island, Antarctica

  • Choi, Ik-Won;Seo, Dong-Chul;Kang, Se-Won;Lee, Sang-Gyu;Seo, Young-Jin;Han, Jong-Hak;Heo, Jong-Soo;Cho, Ju-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.1
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    • pp.103-106
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    • 2012
  • To investigate distribution characteristics of heavy metals in soils in the vicinity of King Sejong Station of King George Island, Antarctica, Fe, Mn, Cu, Zn, Pb, Co, Cd, Cr, Ni and Mo contents were investigated under different distances and directions from King Sejong Station. 13 sample sites were distributed from King Sejong Station. In site 4, the Cd content was higher than that in other sites. The Cr content was the highest in site 9, and the Ni and Mn contents were high in site 12. Cu, Co and Zn were highly contained in distance 1~2 km from King Sejong Station. Cd, Cr, Ni and Mo contents were lower, whereas Fe, Mn, Cu, Zn, Pb and Co contents were higher than other heavy metal contents at all sites.

Integrated Hybrid Device for High-Efficiency Size-Tunable Particle Separation (고효율 크기 가변적 입자 분리를 위한 통합 하이브리드 소자)

  • Choo, Seung Hee;Park, Jion;Kim, Tae Eun;Gang, Tae Gyeoung;An, Jun Seok;Oh, Gayeong;Kim, Yeojin;Park, Kyu Been;Park, Chaewon;Lee, Minjeong;Lim, Hyunjung;Nam, Jeonghun
    • Journal of Biomedical Engineering Research
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    • v.43 no.3
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    • pp.170-176
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    • 2022
  • Cell separation from a heterogenous mixture sample is an essential process for downstream analysis in biological, chemical, and clinical applications. This study demonstrates an integrated hybrid device of the viscoelastic focusing in a straight rectangular channel and subsequent size-based separation using acoustophoresis to attain high efficiency and separation tunability. For particle pre-alignment in a viscoelastic fluid, the flow rate higher than 10 μl/min was required. Surface acoustic wave-based lateral migration of particles with different sizes (13 and 27 ㎛) was examined at various applied voltages and flow rate conditions. Therefore, the flow rate of 100 μl/min and the applied voltage of 20 Vpp can be used for size-based particle separation.

Bragg Reflecting Waveguide Device Fabricated on a Flexible Substrate using a Nano-imprinting Technology (나노임프린팅 기술을 이용한 유연성 브래그 반사 광도파로 소자)

  • Kim, Kyung-Jo;Yi, Jeong-Ah;Oh, Min-Cheol
    • Korean Journal of Optics and Photonics
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    • v.18 no.2
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    • pp.149-154
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    • 2007
  • Bragg reflecting waveguide devices have been fabricated on a flexible polymer substrate utilizing a post lift-off process which could Provide excellent uniformity of grating Patterns on Plastic film. The 510 m Period Bragg grating pattern is made by two methods. In the first sample the grating is fabricated by exposing the laser interference pattern on a photoresist, and then it is inscribed by $O_2$ plasma etching. The grating pattern of the second sample is formed by a PDMS soft mold imprinting process. The selective adhesion property of SU-8 material for Au and Si surfaces is utilized to prepare a 100-mm thick plastic substrate. Single mode waveguide is fabricated on the plastic substrate using polymer materials with refractive indices of 1.540 and 1.430 for the core and the cladding layers, respectively. The Bragg grating on Plastic substrate does not show any degradation in its spectral response compared to the reference sample made on a silicon wafer.

A Study on Microbial Contamination of Foods Exposed to Multiple Environments

  • KIM, Dan-Bee;CHA, Seong-Soo
    • The Korean Journal of Food & Health Convergence
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    • v.5 no.3
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    • pp.35-40
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    • 2019
  • In this study, general bacterial counts and coliform counts, which are hygienic indicator microorganisms, were tested for candy, chocolate, and jelly which are easily available and enjoyed around. After dropping each sample on the desk, indoors, and outdoors, it is immediately collected, or washed and collected to confirm the myth of the 3-second rule. Immediately after removing the wrapping paper, each sample was dropped on the desk, indoors, and outdoors, and after 3 seconds from the moment of contact with the surface, and then collected in a sample bag using sterilized sanitary gloves. After the same operation, each sample was rinsed for 5 seconds using sterilized sanitary gloves and sterilized distilled water, and then collected in a sample bag. The number of bacteria detected in non-washing candies was 41 CFU/g at outdoor and the number of bacteria detected in non-washing chocolate was 76 CFU/g at outdoor. The number of bacteria detected in non-washing jellies was 79 CFU/g at outdoor. Coliform group was not detected in all samples. This showed good results at the level of m = 10,000 or less, which is an allowable value suggested in the Food Code. Also, effect of washing on contaminated food was confirmed. This result is remarkably low compared with the microorganism specimens shown in Food Code, and it is confirmed that contamination occurs but not high value. Therefore, the myth of the 3-second rule is true compared to the figures based on Food Code. However, it showed the characteristics of bacteria that could survive and cross-contaminate on dry food surfaces and emphasized the importance of hygiene through food contact to unsanitary surfaces to minimize the risk of food poisoning.

Optimization of Ethanol Extraction Conditions from Glasswort (Salicornia herbacea) Using Response Surface Methodology (반응표면분석법을 이용한 퉁퉁마디 에탄올 추출조건의 최적화)

  • Park, Jeong-Wook;Kim, Hae-Seop;Park, In-Bae;Shin, Gung-Won;Lee, Young-Jae;Jo, Yeong-Cheol
    • Food Science and Preservation
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    • v.16 no.3
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    • pp.376-384
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    • 2009
  • Response surface methodology (RSM) was used to monitor the characteristics of ethanol extracts from glasswort (Salicornia herbacea). A central composite design was used to investigate the effects of the independent variables of sample ratio, extraction temperature, and ethanol concentration on the dependent variables color, sugar, salinity, yield, electron donating ability, and total polyphenol content of extracts. The maximum $^{\circ}Brix$ (8.46) was obtained under specific extraction conditions, with a sample ratio of 7.04 g/100 mL, an extraction temperature of $89.01^{\circ}C$, and an ethanol concentration of 34.29% v/v. At a sample ratio, extraction temperature, and ethanol concentration of 7.00 g/100 mL, $89.15^{\circ}C$, and 34.14% v/v, respectively, the salinity was 7.35%. When the sample ratio, extraction temperature, and ethanol concentration were 5.56 g/100 mL, $68.61^{\circ}C$, and 99.14% v/v, respectively, the maximum electron donating ability was 86.10%. A maximized total polyphenol content of 1,140.15 mg/100 g was found with the following conditions: sample ratio of 8.6 g/100 mL, extraction temperature of $64.19^{\circ}C$, and ethanol concentration of 71.74% v/v. Overall, the optimal ranges of extraction conditions for effective components of glasswort were 3.38.5.33 g/100 mL sample ratio, $55.87-76.96^{\circ}C$, and 25.00.67.31% v/v ethanol.

Detection of Canine Lymphoma by the Amplification of Antigen Receptor Gene Rearrangements (재배열 항원 수용체 유전자 증폭을 통한 개 림프종의 진단)

  • Yu, Do-Hyeon;Li, Ying-Hua;Lee, Jong-Hyun;Noh, Dong-Ho;Song, Ru-Hui;Lee, Mi-Jin;Choi, Ul-Soo;Park, Jin-Ho
    • Journal of Veterinary Clinics
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    • v.26 no.5
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    • pp.419-422
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    • 2009
  • We performed the PARR (PCR to detect antigen receptor rearrangements) test on DNA isolated from twelve archival canine cytological slides including nine lymphoma, two reactive lymphocytes and one sample from Ehrlichia canis infected dog. As a result, our PCR control gene, $C{\mu}$, was successfully amplified from all of the DNA samples. Six out of nine lymphoma samples showed a clonal rearrangement of immunoglobulin gene whereas three samples did a clonal rearrangement of T cell receptor gamma ($TCR{\gamma}$) gene. However, we observed no visible or clear bands from PCR conducted using our antigen receptor rearrangement primers on DNA from a reactive lymphoid cell proliferation used as a negative control. False-positive amplification in $TCR{\gamma}$ gene was observed only in one sample from E. canis infection. The use of archival cytological specimens demonstrated in this study offers potential advantages for cost-effective specimen acquisition and efficient high-fidelity DNA analysis.

A Study on the Yield of Functional Components of Citrus Peel Extracts using Optimized Hot Water Extraction and Enzymatic Hydrolysis (열수추출 및 효소처리에 따른 감귤 과피 추출물의 기능성성분 수득에 대한 연구)

  • Noh, Jung-Eun;Yoon, Sung-Ran;Lim, Ae-Kyoung;Kim, Hye-Jeong;Huh, Dam;Kim, Dae-Ik
    • Korean journal of food and cookery science
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    • v.28 no.1
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    • pp.51-55
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    • 2012
  • This study was conducted to investigate the physicochemical properties of citrus peel extracts with different hot water extraction and enzymatic hydrolysis conditions. Enzymatic hydrolysis was also employed using Viscozyme L and results were compared with that of optimized hot water extract. Hot water extraction was performed under different parameters; the sample to solvent ratio(1:20, 1:15, 1:10), extraction time(2, 4 hrs), extraction temperature(85, $95^{\circ}C$) and enzymatic hydrolysis(0, 1%) and the subsequent extracts were used for determining their physicochemical properties, such as total yield, total phenolics, total flavonoids, and electron donating ability (EDA). With the increase in the sample to solvent ratio and extraction time, total yield, total phenolics, total flavonoids and EDA increased. But extraction temperature did not significantly affect the hot water extract. As hot water extract was hydrolyzed by the enzyme, total yield and active ingredients increased rapidly. In the result of total yield, total phenolics, total flavonoids and EDA, the activity of enzyme-treated extract was higher than those of enzyme-untreated extract. Based upon the overall hot water extraction efficiency, it was found that 20 times volume or 120 min at a time at $95^{\circ}C$ after enzyme treatment was optimal.