• 대한원격탐사학회지
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• 제26권2호
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• pp.251-262
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• 2010

GOCI(Geostationary Ocean Color Imager) 표준자료의 지속적인 품질관리를 위해서는 위성 운용기간 중 궤도상 복사보정, 대기보정 단계를 거쳐야 되며 해수환경 분석 알고리즘에 대한 검보정도 지속적으로 이루어져야 한다. GOCI의 복사, 대기, 해양환경 자료에 대한 검보정은 부이나 고정 플랫폼을 이용한 수온, 염분, 해수 광특성, 형광, 및 탁도 관측과, 주기적으로 해양환경 자료 수집을 통하여 실시한다. 이를 위하여 동중국해에 위치하고 있는 이어도 종합해양과학기지에 설치된 광학 관측 장비와 현장 관측의 복사자료를 상호 비교해 보았으며, GOCI 표준자료의 검정에 앞서 SeaWiFS 복사량과 비교하여 검정하였다. 해수출 광량은 현장관측에서 얻어진 광과 광량과는 약간의 차이를 보였지만, 흡광영역이 매우 잘 일치하고 있으며 스펙트럴 이동은 없는 것으로 판단된다. 이어도 종합해양과학기지의 분광측정기와 SeaWiFS의 전 밴드에서 얻어진 해수출 광량을 비교한 결과 평균 25% 정도의 에러가 발생했지만, 대기보정 밴드를 제외하면 절대오차가 11% 정도로 상당히 낮아진다. 이것은 SeaWiFS 표준 대기보정 방법의 문제점으로 GOCI 검보정 연구에서 고려되어 보완 되어야 할 것으로 판단된다. 이와 더불어 독도 지역의 표준 관측치(Reference Target Site) 구축을 통한 검보정 연구를 위하여, 독도 주변 해수의 광 특성과 해양환경 자료는 2009년 8월과 2009년 10월 2차례에 걸쳐서 현장관측을 실시하였다. 독도 주변 해역의 해양 광 특성은 원격반사도의 스펙트럼형태를 기준으로 Case-1 Water 성향이 강한 해수에서 나타나는 특성과 매우 유사하였다. 식물플랑크톤, 부유물질, 용존유기물의 흡광계수 스펙트럼의 형태들은 대체적으로 각 성분별 흡광 스펙트럼 특성을 잘 보여주었다. 또한 MODIS Aqua로부터 산출된 엽록소 농도와 현장관측을 통한 검증에서 위성자료 값들은 잘 일치한다. 위와 같이 현재 진행되고 있는 GOCI 검보정 연구를 통해서 복사, 대기, 해양환경 알고리즘에 대한 문제점이 도출되었고, 차후 검보정 계획에 반영하여 이 부분들에 대한 개선 및 보완이 이루어질 것으로 판단된다.

• Journal of Nutrition and Health
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• 제55권6호
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• pp.601-616
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• 2022

In the era of the fourth industrial revolution technology, the inclusion of personalized nutrition for healthcare (PNH), when establishing a healthcare platform to prevent chronic diseases such as obesity, diabetes, cerebrovascular and cardiovascular disease, pulmonary disease, and inflammatory diseases, enhances the national competitiveness of global healthcare markets. Furthermore, since the government experienced COVID-19 and the population dead cross in 2020, as well as numerous health problems due to an increasing super-aged Korean society, there is an urgent need to secure, develop, and utilize PNH-related technologies. Three conditions are essential for the development of PNH technologies. These include the establishment of causality between obesity genome (genotype) and prevalence (phenotype) in Koreans, validation of clinical intervention research, and securing PNH-utilization technology (i.e., algorithm development, artificial intelligence-based platform, direct-to-customer [DTC]-based PNH, etc.). Therefore, a national control tower is required to establish appropriate PNH infrastructure (basic and clinical research, cultivation of PNH-related experts, etc.). The post-corona era will be aggressive in sharing data knowledge and developing related technologies, and Korea needs to actively participate in the large-scale global healthcare markets. This review provides the importance of scientific evidence based on a huge dataset, which is the primary prerequisite for the DTC obesity gene-based PNH technologies to be competitive in the healthcare market. Furthermore, based on comparing domestic and internationally approved DTC obese genes and the current status of Korean obesity genome-based PNH research, we intend to provide a direction to PNH planners (individuals and industries) for establishing scientific PNH guidelines for the prevention of obesity.

• Journal of Microbiology and Biotechnology
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• 제28권11호
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• pp.1937-1945
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• 2018

Malassezia pachydermatis is a commensal yeast found on the skin of dogs. However, M. pachydermatis is also considered an opportunistic pathogen and is associated with various canine skin diseases including otitis externa and atopic dermatitis, which usually require treatment using an azole antifungal drug, such as ketoconazole. In this study, we isolated a ketoconazole-resistant strain of M. pachydermatis, designated "KCTC 27587," from the external ear canal of a dog with otitis externa and analyzed its resistance mechanism. To understand the mechanism underlying ketoconazole resistance of the clinical isolate M. pachydermatis KCTC 27587, the whole genome of the yeast was sequenced using the PacBio platform and was compared with M. pachydermatis type strain CBS 1879. We found that a ~84-kb region in chromosome 4 of M. pachydermatis KCTC 27587 was tandemly quadruplicated. The quadruplicated region contains 52 protein coding genes, including the homologs of ERG4 and ERG11, whose overexpression is known to be associated with azole resistance. Our data suggest that the quadruplication of the ~84-kb region may be the cause of the ketoconazole resistance in M. pachydermatis KCTC 27587.

• 한국문헌정보학회지
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• 제56권3호
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• pp.265-290
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• 2022

본 연구는 한국과학기술정보연구원의 OA논문 통합서비스플랫폼(Discover)에 대한 비용편익분석을 통해 사업의 경제성 평가를 수행하였다. 학술연구자 1,313명, 바이오산업 연구개발자 49명, 일반 산업체 연구개발자 102명 등 서비스 수혜 집단별로 설문조사를 실시하여 편익측정에 필요한 기초자료를 수집하였다. 시스템 구축과 운영에 소요되는 비용, 직접편익과 간접편익을 추정한 후, 이를 토대로 비용편익분석과 민감도 분석을 실시하였다. 우선 직접 편익에 한정한 비용편익분석 결과, 총 8년간 사업에 투입된 사업투자비용(약 56.3억)의 약 14배인 820억 원의 편익이 발생할 것으로 추정되었다. 직간접편익을 합산한 비용편익분석 결과 역시 BCR이 최소 98.9, NPV는 5,388억 원 등으로 추정되어 사업의 경제적 타당성이 충분히 확보되었다. 본 분석 결과는 국가 차원의 OA논문 통합서비스플랫폼 개발 및 구축의 타당성 확보에 기여할 수 있으며, 본 연구에서 개발된 편익추정모형은 향후 사업 진행 과정에서 평가도구로 활용될 수 있을 것이다.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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• pp.151-155
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• 2005

Recently, systems biology has been increasingly applied to gain insights into the complexity of living organisms. Many inaccessible biological information and hidden evidences fur example flux distribution of the metabolites are simply revealed by investigation of artificial cell behaviors. Most bio-models are models of single cell organisms that cannot handle the multi-cellular organisms like plants. Herein, a structured and multi-cellular model of potato was developed to comprehend the root starch biosynthesis. On the basis of simplest plant cell biology, a potato structured model on the platform of Berkley Madonna was divided into three parts: photosynthetic (leaf), non-photosynthetic (tuber) and transportation (phloem) cells. The model of starch biosynthesis begins with the fixation of CO$_2$ from atmosphere to the Calvin cycle. Passing through a series of reactions, triose phosphate from Calvin cycle is converted to sucrose which is transported to sink cells and is eventually formed the amylose and amylopectin (starch constituents). After validating the model with data from a number of literatures, the results show that the structured model is a good representative of the studied system. The result of triose phosphate (DHAP and GAP) elevation due to lessening the aldolase activity is an illustration of the validation. Furthermore, the representative model was used to gain more understanding of starch production process such as the effect of CO$_2$ uptake on qualitative and quantitative aspects of starch biosynthesis.

• Genomics & Informatics
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• 제17권3호
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• pp.28.1-28.9
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• 2019

Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification of genes required for growth in any condition of interest. Anti-sense strands of amplified bar-codes hybridize with ~10,000 (5,000 each for up-and down-tags) different kinds of sense-strand probes on an array. In this study, we optimized the hybridization processes of an array for fission yeast. Compared to the first version of the array (11 ㎛, 100K) consisting of three sectors with probe pairs (perfect match and mismatch), the second version (11 ㎛, 48K) could represent ~10,000 up-/ down-tags in quadruplicate along with 1,508 negative controls in quadruplicate and a single set of 1,000 unique negative controls at random dispersed positions without mismatch pairs. For PCR, the optimal annealing temperature (maximizing yield and minimizing extra bands) was 58℃ for both tags. Intriguingly, up-tags required 3× higher amounts of blocking oligonucleotides than down-tags. A 1:1 mix ratio between up- and down-tags was satisfactory. A lower temperature (25℃) was optimal for cultivation instead of a normal temperature (30℃) because of extra temperature-sensitive mutants in a subset of the deletion library. Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. A tag intensity analysis showed that tag(s) of 4,316 of the 4,526 strains tested were represented at least once; 3,706 strains were represented by both tags, 4,072 strains by up-tags only, and 3,950 strains by down-tags only. The results indicate that this microarray will be a powerful analytical platform for elucidating currently unknown gene functions.

• Genomics & Informatics
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• 제16권2호
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• pp.22-29
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• 2018

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 strains) covering 88.5% of all 4,919 open reading frames, 7,734 barcodes (88.8%) were validated as high-fidelity to be inserted at the correct positions by Sanger sequencing. Sequence examination of the 7,734 high-fidelity barcodes revealed that 1,039 barcodes (13.4%) deviated from the original design. In total, 1,284 mutations (mutation rate of 16.6%) exist within the 1,039 mutated barcodes, which is comparable to budding yeast (18%). When the type of mutation was considered, substitutions accounted for 845 mutations (10.9%), deletions accounted for 319 mutations (4.1%), and insertions accounted for 121 mutations (1.6%). Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. When the mutation rate was analyzed by position within 20-mer barcodes using the 1,284 mutations from the 7,734 sequenced barcodes, there was no significant difference between up-tags and down-tags at a given position. The mutation frequency at a given position was similar at most positions, ranging from 0.4% (32/7,734) to 1.1% (82/7,734), except at position 1, which was highest (3.1%), as in budding yeast. Together, well-defined barcode sequences, combined with the next-generation sequencing platform, promise to make the fission yeast gene deletion library a powerful tool for understanding gene function.

• 한국패류학회지
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• 제30권4호
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• pp.429-431
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• 2014

본 연구를 통하여 연체동물 전용 BLAST 서버 (Version II)가 웹주소 http://www.malacol.or.kr/blast에 구축되었다. 연체동물을 대상으로 하는 연구에 있어 필요한 정보를 매우 빠르게 얻을 수 있었다. 본 시스템을 사용하여 앞으로도 많은 연구가 진행되어지길 바라며, 아울러 많은 연체동물 연구자들에게 많은 도움이 되리라고 사료된다.