• 제목/요약/키워드: Binding Force

검색결과 187건 처리시간 0.025초

자성체를 이용한 단백질칩 마이크로어레이의 개발 (Development of Protein Chip Microarray Using a Magnetic)

  • 최용성;이경섭;박대희
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2005년도 제36회 하계학술대회 논문집 C
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    • pp.2398-2400
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    • 2005
  • In this paper, we have been described a new constructing method of multichannel biosensor using self-assembly by magnetic force interaction. A metal particle and an array was fabricated by photolithographic. Biomaterials were immobilized on the metal particle. The array and the particles were mixed in a buffer solution, and were arranged by magnetic force interaction and self-assembly. A quarter of total Ni dots were covered by the particles. The binding direction of the particles was controllable, and condition of particles was almost with Au surface on top. The particles were successfully arranged on the array. The biomaterial activities were detected by chemiluminescence.

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자성체 어레이를 이용한 단백질칩 (Protein Chip by Magnetic Array)

  • 최용성;이경섭;박대희
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2005년도 하계학술대회 논문집 Vol.6
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    • pp.426-427
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    • 2005
  • This research describes a new constructing method of multifunctional biosensor using many kinds of biomaterials. A metal particle and an array was fabricated by photolithographic. Biomaterials were immobilized on the metal particle. The array and the particles were mixed in a buffer solution, and were arranged by magnetic force interaction and self-assembly. A quarter of total Ni dots were covered by the particles. The binding direction of the particles was controllable, and condition of particles was almost with Au surface on top. The particles were successfully arranged on the array. The biomaterial activities were detected by chemiluminescence and electrochemical methods.

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자기력에 의한 단백질칩 (Protein Chip Using Magnetic Force)

  • 최용성;문종대;이경섭
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2006년도 하계학술대회 논문집 Vol.7
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    • pp.386-387
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    • 2006
  • This research describes a new constructing method of multifunctional biosensor using many kinds of biomaterials. A metal particle and an array was fabricated by photolithographic. Biomaterials were immobilized on the metal particle. The array and the particles were mixed in a buffer solution, and were arranged by magnetic force interaction and self-assembly. A quarter of total Ni dots were covered by the particles. The binding direction of the particles was controllable, and condition of particles was almost with Au surface on top. The particles were successfully arranged on the array. The biomaterial activities were detected by chemiluminescence and electrochemical methods.

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수분에 의존하는 단백질의 특성이 어묵의 조직에 미치는 영향 (Moisture-dependent Gelation Characteristics of Nonfish Protein Affect the Surimi Gel Texture)

  • Chung, Kang-Hyun;Lee, Chong-Min
    • 한국식품조리과학회지
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    • 제12권4호
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    • pp.571-576
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    • 1996
  • The moisture-dependent gelation characteristics of five different proteins are evaluated to understand the modification of gel strength when they are added in surimi gel. Compressive force and penetration force of protein gels gradually decreased with increase of moisture level, with showing markedly decrease at certain point of moisture level called critical moisture level. The critical moisture level for gelation of SPI-1, SPI-2, EW, WPC and LA were 79.4%, 81.6%, 91.4%, 87,8% and 84.7%, respectively. Beyond this critical level of water, protein gel matrix begins to lose its water binding and structural integrity. The mnisture that was not re tained by a protein was available to diluting the protein matrix and eventually weakened the overall gel strength. EW and MPI showed higher water retention than those of SPI, WPC and LA. The compressive force of SPI, WPC and LA-incorporated surimi gel at the varying moisture levels strongly correlated with the amount if water retained at corresponding moisture level within those protein (r=0.99).

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혼합접착제 적용에 따른 골판지의 접착강도와 건조에너지 평가 (Evaluation of the Adhesive Strength and the Drying Energy of Corrugated Board Using a Mixed Adhesive)

  • 이지영;김철환;김은혜;박태웅;최재성
    • 펄프종이기술
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    • 제48권3호
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    • pp.51-56
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    • 2016
  • The most widely used glue in a corrugator is starch, which is a natural polymer. This material needs thermal energy to achieve a binding force, so a heating section is installed in a corrugator. However, this heating section can cause quality problems in linerboards and corrugating medium and increase the production cost because of the high cost of fossil resources. Therefore, a new adhesive that provides the binding force at lower temperatures than the conventional one must be developed. In this study, SB-latex was selected as a co-adhesive and added to the starch solution. The addition of the SB-latex was determined based on the viscosity of the new adhesive. The adhesive strength and the drying energy reduction of a corrugated board were measured to evaluate the functionalities of the new adhesive. The addition of SB-latex was determined to be under 20% of the oven-dried starch based on the viscosity of the new adhesive. The adhesive strength was improved and the drying energy was reduced by applying the new adhesive.

pH, 온도, 단백질함량에 따른 돼지혈장 단백질의 보수력 변화 (Effects of pH, Temperature, and Protein Content on Water Binding Capacity of Hog Plasma Protein)

  • 김주봉;이영현
    • 한국식품영양과학회지
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    • 제18권2호
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    • pp.195-198
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    • 1989
  • 도축장에서는 폐기되는 돼지 혈액으로부터 혈장을 분리하여, pH, 온도, 단백질함량에 따른 혈장 단백질 보수력의 변화를 조사하였다. 돼지 혈액으로부터 혈장분리는 1400g-force에서 가장 좋았다. 단백질 농도가 5%인 혈장액을 $85^{\circ}C$에서 30분간 가열하였을 때 pH가 증가함에 따라 혈장단백질보수력은 급격히 증가하다가 pH 7 이후에는 완만한 증가를 나타냈다. 단백질 농도가 5%이고 pH 7인 혈장액에서의 보수력은 gel화 온도가 높을수록 짧은 시간 내에 높은 보수력을 나타냈으며 가열 초기에 급격히 증가하다가 일정 가열 시간 이후에는 큰 변화를 나타내지 않았다. pH가 7인 혈장액을 $85^{\circ}C$에서 30분간 가열하였을 때 혈장 단백질의 농도가 증가함에 따라 단위 단백질 무게당 보수력은 감소하였다.

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Surface Plasmon Resonance Immunosensor for Detection of Legionella pneumophila

  • Oh, Byung-Keun;Lee, Woochang;Bae, Young-Min;Lee, Won-Hong;Park, Jeong-Woo
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제8권2호
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    • pp.112-116
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    • 2003
  • An immunosensor based on surface plasmon resonance (SPR) onto a protein G layer by Self-assembly technique was developed for detection of Legionella pneumophila. The protein G layer by self-assembly technique was fabricated on a gold (Au) surface by adsorbing the 11-mercaptoundecanoic acid (MUA) and an activation process for the chemical binding of the free amino (-NH$_2$) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of the protein G layer by self-assembly technique on the Au Substrate and the binding of the antibody and antigen in series were confirmed by SPR spectroscopy. The Surface topographies of the fabricated thin films on an Au substrate were also analyzed by using an atomic force microscope (AFM). Consequently, an immunosensor for the detection of L. pneumophila using SPR was developed with a detection limit of up to 10$^2$CFU per mL.

The Interaction between Hexadecyltrimethylammonium Bromide to Poly (Ethylene Glycol) with Different Molecular Weights and Some Schiff-Bases Investigated by Surfactant Ion Selective Electrode

  • Ghoreishi, Sayed Mehdi;Naeimi, Hossein;Navid, Mohammad Davodi
    • Bulletin of the Korean Chemical Society
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    • 제26권4호
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    • pp.548-552
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    • 2005
  • The interaction between ionic surfactants and different nonionic molecules and polymers are studied using ion surfactant selective electrode. From the experimental data, critical concentrations of the interaction and binding process are evaluated. The interaction between hexadecyltrimethylammonium bromide (HTAB) with polyethylene glycol (PEG) in three molecular weights (1000, 10000 and 100000) and also schiff-bases, 2-[2-carboxyphenyl nitrilomethylidyne]-phenol (ortho CNP), 2-[3-carboxyphenyl nitrilomethylidyne]-phenol (meta CNP)and 2-[4-carboxyphenyl nitrilomethylidyne]-phenol (para CNP) with the potentiometric method were investigated using HTAB membrane selective electrode. In the case of PEG with increasing molecular weights more interaction to HTAB occurs. The electromotive force (EMF) data also showed that interaction between para CNP with HTAB is more than the other schiff-bases. It seems this case related to less space interference of COOH group for that compound. The onset of binding ($T_1$) of course is the same for three schiffbase molecules.

Interaction of Bilobalide and Ginkgolides B with Bovine Serum Albumin: A Fluorescence Quenching Study

  • Chen, Yan;Wang, Ruijun;Wang, Shusheng;Yang, Yi;Li, Shaofei;Kai, Guiqing
    • Bulletin of the Korean Chemical Society
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    • 제32권9호
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    • pp.3261-3266
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    • 2011
  • The interaction of bilobalide (BB) and ginkgolides B (GB) with bovine serum albumin (BSA) was investigated by fluorescent technique and UV/vis absorption spectroscopy. The results showed that BB and GB could intensively quench the fluorescence of BSA through a static quenching procedure. The binding constants (Ka) and the average binding distance between the donor (BSA) and the acceptor (ginkgolides) were obtained ($r_{BB}$ = 5.33 nm and $r_{GB}$ = 4.20 nm) by the theory of non-radiation energy transfer, and then the thermodynamic parameters such as ${\Delta}S^0$ (0.17-0.32 kJ/mol), ${\Delta}G^0$ (-20.76 ~ -17.79 kJ/mol) and ${\Delta}H^0$ (32.47-76.52 kJ/mol) could be calculated, respectively. All these results revealed that the interaction of BB and GB with BSA were driven mainly by hydrophobie force. The synchronous fluorescence spectroscopy was applied to examine the effect of two ginkgolides on the configuration of BSA. The configuration alteration of BSA could be induced by the hydrophobicitv environment of tyrosine with the increase of the drug concentration.

The Effects of O2 Plasma Treatment on Electrical Properties of Graphene Grown by Chemical Vapor Deposition

  • 김윤형;박진섭
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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    • pp.384.2-384.2
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    • 2014
  • We investigated the electrical and structural properties of chemical vapor deposition (CVD)-grown graphene and post treated by O2 plasma. For the patterning of graphene, the plasma technology is generally used and essential for etching of graphene. But, the cautious O2 plasma treatments are required to avoid the damage in graphene edge which can be the harmful effects on the device performance. To analyze the effects of plasma treatment on structural properties of graphene, the change of surface morphology of graphene are measured by scanning electron microscope and atomic force microscope before and after plasma treatment. In addition, the binding energy of carbon and oxygen are measured through to X-ray photoelectron spectroscopy. After plasma treatment, the severe changes of surface morphology and binding energy of carbon and oxygen were observed which effects on the change of sheet resistance. Finally, to analyze of graphene characteristics, we measured the Raman spectroscopy. The measured results showed that the plasma treatment makes the upward of D-peak and downward of G'-peak by elevated power of plasma.

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