• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.887-893
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• 2007

Influences of forage DM and addition of cane molasses on silage characteristics of berseem (Trifolium alexandrium) and lucerne (Medicago sativa) and their ruminal digestion kinetics in Nili buffaloes were studied. Berseem and lucerne fodders (at one tenth bloom) were ensiled with wheat straw in laboratory silos to achieve 20, 30, 40% forage DM and without wheat straw (control); each forage DM level was supplemented with 2, 4 and 6% of cane molasses at ensiling. The pH and lactic acid contents of berseem and lucerne silages were affected by both forage DM and addition of molasses. Dry matter, CP and true protein (TP) of berseem and lucerne silages were affected by forage DM at ensiling but were not affected by the addition of cane molasses. Higher DM, CP and TP losses were observed when berseem and lucerne fodders were ensiled either without wheat straw or with wheat straw to achieve 20% and 40% forage DM at ensiling compared with 30% DM at ensiling. Fiber fractions (NDF, ADF, hemicellulose and cellulose) of berseem silage and lucerne silage were significantly increased with increasing forage DM at ensiling. Addition of cane molasses did not affect the DM, CP, TP and fiber fractions of both berseem and lucerne silages. Berseem and lucerne ensiled at 30% DM with 2% cane molasses were screened for comparative ruminal digestion kinetics with their respective fodders. Addition of wheat straw to berseem or lucerne fodder at ensiling depressed DM and NDF ruminal degradability. However, ruminal lag time, rate of degradation and extent of digestion of silages were similar to their respective fodders. In conclusion, berseem and lucerne could be ensiled with wheat straw to increase their DM to 30% along with 2% molasses for buffaloes.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.371-375
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• 1999

The present study was conducted to evaluate the mottgrass alone and in combination with berseem (Trifolium alexandrinum) at different intake levels to determine the lag time, rate and extent of digestion of DM and NDF of mottgrass. Four ruminally cannulated buffalo calves were used in a $4{\times}4$ Latin Square Design with $2{\times}2$ factorial arrangement of treatments. The two factors were forage intake levels (ad libitum versus restricted) and forage source (mottgrass versus mottgrass plus 25% berseem). Four dietary treatment consisted of 1) ad libitum mottgrass, 2) restricted feeding of mottgrass, 3) ad libitum mottgrass plus berseem with a ratio of 3:1 and 4) restricted mottgrass plus berseem in a ratio of 3:1 Calves fed ad libitum mottgrass supplemented with 25% berseem consumed 25% more DM and 15 % more NDF than those fed mottgrass only. The in situ DM digestibility, the lag time and extent of digestion were not affected by intake level. However, rate of disappearance was greater in restricted fed animals than those fed ad libitum. This increased rate could be due to greater concentration of fibrolytic bacteria in restrict fed animals. The DM digestibility was greater (64.1%) in calves fed mottgrass supplemented with 25% berseem than those fed mottgrass only (57.7%). The reduced mottgrass DM digestion may be due to its higher NDF contents. The NDF digestibility, the lag and extent of NDF digestion were not affected by varying intake levels. However, rate of digestion of NDF was higher in restricted fed animals than those of ad libitum fed animals. The NDF digestibility was greater (58.4%) in calves fed mottgrass supplemented with 25% berseem than those fed mottgrass (48.7%) only.

• Asian-Australasian Journal of Animal Sciences
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• 제12권7호
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• pp.1040-1048
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• 1999

Four ruminally fistulated crossbred $(Sahiwal{\times}Holstein\;Friesan)$ adult (~6 yr.) cattle of about $318{\pm}16kg$ body weight were randomly assigned in an experiment based on $4{\times}4$ latin square design to study the effect of different level of barseem (Trifolium alexendrinum) supplementation to wheat straw based diet on intake, digestion, VFA production, eating behaviour and passage rate. Four dietary treatments were wheat straw ad lib. (I), supplementation to wheat either 15(II), 30(III) or 45(IV)% of berseem. Mean total volatile fatty acids (TVFA) concentration in rumen liquor was 58.45, 66.14, 77.92 and 78.64 mmol/l. TVFA concentration in rumen liquor increased significantly (p<0.01) with increased level of berseem supplementation upon 30% level of breseem. Two peaks of TVFA concentration was observed at 4 and 8 h post feeding. Brseem supplementation showed no significant effect on daily time spent for eating, ruminating or idling, chews per minute or number of rumination boli ingested per minute. Time spent for eating and ruminating one kg neutral detergent fibre (NDF) was significantly (p<0.05) less in breseem supplemented groups. Animals in groups I, II, III and IV consumed 26, 34, 47 and 57% of DM within 1st 4 h and 64, 70, 70 and 77% of total DM within 1st 8 h of offering. All the animals consumed more than 90% of their DMI within 12 h. Active period of rumination was observed 8h post feeding reached the peak at 16 h post feeding, then declined and animals spend considerable time idling in last 4 h of 24 h feeding cycle. Berseem supplementation showed no significant effect on eating and rumination time. It is concluded that berseem supplementation upon 30% increases the efficiency of chewing during eating and rumination, which results in increase intake and TVFA production and nutrient digestibility.

• 한국초지조사료학회지
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• 제15권2호
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• pp.80-86
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• 1995

High salinity common in reclaimed soil can reduce germination of crop seeds and aftermath its establishment and production. The experiment was done to measure germination and radicle elongation of berseem, subterranean, white clover and Italian ryegrass as Control under different temperature and NaCl concentrations. Onehundred seeds of berseem (Trifolium ulexandrinum cv. Bigbee). suberranean (T. suhterruneum, cv. Nungarin), white clover (T. repens, cv. Regal) and Italian ryegrass (Lolium mulriflorum, cv. Terafloum) were placed in petri dishes with a sheet of filter paper replaced every two days, and then exposed to 0, 12.5, 25. 50, 100 and 200mh4 of sodium chloride in darkened growth chambers controlled with 10, 15 and $20^{\circ}C$. Percent germination and radicle elongation were measured. Beneem clover showed greatest daily percent germination among the cloven. That of subterranean clover, moreover, was higher than that of white clover till 4 days after imbibition but the reverse result was true thereafter. Although germination of Italian ryegrass was delayed with decreased at 200mM of NaCl of $20^{\circ}C$. at 200mM of under$15^{\circ}C$ , and at higher than lOOmh4 of all level of temperature, respectively. Berseem clover had greatest radicle length under the same concentration of NaCl regardless of temperature treatment while radicle elongation of subterranean and white clover was repressed over 50-100mM of NaC1. The slope of linear regression equation between concentration of NaCl and percent germination declined under all temperature treatment in order of berseem, subterranean and white clover whereas the reverse result was showed in radicle length.

• Asian-Australasian Journal of Animal Sciences
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• 제18권4호
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• pp.475-478
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• 2005

This study was conducted to evaluate feeding value of berseem and lucerne silage as a replacement for conventional fodder (berseem fodder) in lactating Nili buffaloes. Fifteen early lactating multi-parous Nili buffaloes, five buffaloes in each group were allotted three experimental diets. Berseem and lucerne fodders were ensiled at 30% DM (wheat straw was used to adjust the DM of fodders) with molasses (at the rate of 2% of fodder DM) in two bunker silos for 30 days. The diets contained 75% DM from berseem fodder (BF), 75% DM from berseem silage (BS) and 75% DM from lucerne silage (LS). Each diet contained 25% concentrate DM. Diets were mixed daily and fed twice a day at ad libitum intakes. Dry matter intake (DMI) was significantly higher (13.8 kg/d) in buffaloes fed BF diet than those fed LS (12.5 kg/d) and BS (11.9 kg/day) diets. The differences in digestible DMI and DMI as percent body weight were significant between fodder and silage based diets but non-significant when BS and LS were compared. Lower DMI with silage-based diets was probably because of low silage pH. Intake of NDF (NDFI) was higher (5.68 kg/d) in buffaloes fed BF diet followed by those fed LS (5.50 kg/d) and BS (5.00 kg/d) diets. The difference was significant (p<0.05) across fodder and silage based diets but NDFI was non-significant across both silage-based diets. The apparent DM digestibility was significantly different (p<0.05) between fodder and silage-based diets but was non-significant between LS and BS diets. Four percent fat corrected milk yield was significantly different (p<0.05) between fodder and silage-based diets but was non-significant between LS and BS diets. Higher milk yield with fodder based diet was because of more digestible nutrient intake (Table 3) compared with silage based diets. Milk CP, TP and NPN and SNF did not show any treatment effects. The present results indicated that the berseem and lucerne fodder ensiled at 30% DM level with 2% molasses could safely replace (75% DM) the conventional leguminous fodder in the diets of lactating Nili buffaloes.

• 한국작물학회지
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• 제37권3호
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• pp.274-282
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• 1992

Annual forage crops have been increasingly important for conpensating insufficient forage production of perennial pastures took place for short interval. This experiment was conducted to determine the effect of sward composition and NㆍP fertilization on forage production and intercompetition of subterranean clover-Italian ryegrass and berseem clover-Italian ryegrass. The two clovers were grown in the field at the clover/ryegrass ratios of 0/100, 25/75, 50/50, 75/25 and 100/0 where no, N (200 kg /ha), P (50 kg /ha) or NㆍP fertilization was done. Each crop was separated after harvest and drying. Relative Crowding Coefficient (RCC), aggressivity and Relative Yield Total (RYT) were analyzed on the basis of the harvested dry matter of each crop. Berseem clover-ryegrass mixtures produced greater yield than subclover-ryegrass mixtures as a result of higher yields of the two component species. In the former forage yield was increased with increased rate of clover up to 75%, while in the latter the highest yields were obtained at more than 50% of the clover. In the mixtures N stimulated the growth of ryegrass, whereas P did only that of subclover. The two clovers produced more forage than the companion grass under no and only P fertilization although the reverse result was true under N or NㆍP fertilization, but the annual forage yield was decreased in the order of N and P, N, P, and no fertilization. The mixture yields were overyielded compared to the Expected Yield. Although generally RCCs and RYTs of subclover were higher than those of berseem clover in the mixtures differing the composition rate or under no and only P fertilization, those of the former clover were lower under N or NㆍP fertilization. In the clover-ryegrass mixtures, ryegrass acted as an aggressor and became more aggressive under P fertilization.

• Asian-Australasian Journal of Animal Sciences
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• 제7권2호
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• pp.191-196
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• 1994

Two experiments were conducted to study the effect of using Egyptian mallow leaf meal (EMLM) on growth performance and feed utilization of common carp (Cyprinus carpio L.) in experiment 1. Four diets containing 0, 5, 15 and 25% EMLM were included at the expense of berseem leaf meal and fed to fingerlings of common carp for 98 days. The results showed that the average daily gain, feed intake and feed coefficient ratio (FCR) were improved (p<0.05) with increasing the level of EMLM in the diet. Fish fed 25% EMLM were similar to control fish (30% berseem leaf meal) in the productive value (PPV%). In experiment 2, five diets were prepared to contain: 1) 30% berseem leaf meal, 2) 25% untreated EMLM, 3) 25% cooked EMLM 4) 25% treated EMLM with 0.5% NaOH and 5) 25% treated EMLM with 1% NaOH. The results showed that diet containing EMLM gave the best growth performance and feed utilization. However, diet containing 1% NaOH treated EMLM superior to the other diets in PPV% and energy utilization. Diets containing 0.5% NaOH-treated EMLM or cooked EMLM decreased the protein utilization compared to those containing EMLM.

• Asian-Australasian Journal of Animal Sciences
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• 제7권2호
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• pp.183-189
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• 1994

Two experiments were made. In experiment 1, four diets containing 0, 5, 15 and 25% washed seaweed meal were prepared to study the effects of incorporating seaweed meal instead of equivalent amounts of berseem leaf meal in fish feeds on growth performance and feed utilization of common carp. The results showed that average daily gain (ADG), specific growth rate (SGR%), dry matter (DM) and ether extract (EE) of the carcasses were decreased (p<0.05) with the increasing level of seaweed meal in the feeds. Inclusion of 5% seaweed meal in the diet gave the best feed conversion ratio (FCR) among all diets, however, protein productive value (PPV) and energy utilization (EU) were decreased (p<0.05) with increasing level of seaweed meal in the diet. In experiment 2, washed seaweed meal was either steam cooked or sprayed with NaOH (0.5% or 1% NaOH) and incorporated in the diets at the level of 25% instead of equivalent amount of berseem leaf meal. The results showed that steam cooked seaweed gave the best (p<0.05) growth performance, FCR and protein efficiency ratios, PER and PPV, for other treatments in descending order were NaOH treated seaweed, washed seaweed and unwashed seaweed.

• 한국초지조사료학회지
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• 제28권1호
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• pp.13-18
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• 2008

본 시험은 우선 논에서 여름철 사료작물과 연계할 수 있는 겨울철 재배에 적합한 월년생 두과작물로 이듬해의 개화를 통한 경관효과와 수량이 우수한 초종의 선발을 목적으로 충남 천안시 성환읍에 위치한 벼재배 농가포장에서 2006년 9월부터 2007년 5월까지 수행되었다. 월년생 두과 사료작물로서 Hairy vetch(Vicia villsa), Crimsom clover(Trifolium incarnatum), Berseem clover(Trifolium alexandrinum), Persian clover(Trifolium resupinatum), Balansa clover(Trifolium michelianum), Sweet clover(Melilotus officinalis) 및 Forage pea(Pisum sativum) 각 1품종을 공시하였다. 파종 45일후 출현율은 Crimsom clover, Hairy vetch, Forage pea 및 Berseem clover가 90%이상으로 높았으며 월동율은 Hairy vetch와 Crimsom clover가 각각 98%와 95%로 가장 높았다. 경관과 관계되는 개화율은 수확시(5월 10일)에 Crimson clover가 100%로 조사되었다. 생초수량과 건물수량은 Crimson clover가 각각 72,556 kg/ha과 16,062 kg/ha으로 가장 높았다. 조단백질 수량은 Hairy vetch가 2,929 kg/ha으로 가장 높았지만 Crimson clover(2,169 kg/ha)와는 차이가 없었다(p<0.05). TDN 수량은 Crimson clover가 9,007 kg/ha으로 가장 높았지만 Hairy vetch(7,366 kg/ha)와는 차이가 없었다(p<0.05). 이상의 결과로부터 월동율, 수량 그리고 경관 등을 고려할 때 Crimson clover가 가장 적합한 것으로 사료되지만 추후 보완 연구가 필요하다고 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권3호
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• pp.357-364
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• 2014

The study evaluated replacement of Egyptian berseem clover (BC, Trifolium alexandrinum) with spent rice straw (SRS) of Pleurotus ostreatus basidiomycete in diets of lactating Baladi goats. Nine lactating homo-parity Baladi goats (average BW $23.8{\pm}0.4$ kg) at 7 d postpartum were used in a triplicate $3{\times}3$ Latin square design with 30 d experimental periods. Goats were fed a basal diet containing 0 (Control), 0.25 (SRS25) and 0.45 (SRS45) (w/w, DM basis) of SRS. The Control diet was berseem clover and concentrate mixture (1:1 DM basis). The SRS45 had lowered total feed intake and forages intake compared to Control. The SRS25 and SRS45 rations had the highest digestibilities of DM (p = 0.0241) and hemicellulose (p = 0.0021) compared to Control which had higher (p<0.01) digestibilities of OM (p = 0.0002) and CP (p = 0.0005) than SRS25 and SRS45. Ruminal pH and microbial protein synthesis were higher (p<0.0001) for SRS25 and SRS45 than Control, which also had the highest (p<0.0001) concentration of TVFA, total proteins, non-protein N, and ammonia-N. All values of serum constituents were within normal ranges. The Control ration had higher serum globulin (p = 0.0148), creatinine (p = 0.0150), glucose (p = 0.0002) and cholesterol (p = 0.0016). Both Control and SRS25 groups had the highest (p<0.05) milk (p = 0.0330) and energy corrected milk (p = 0.0290) yields. Fat content was higher (p = 0.0373) with SRS45 and SRS25 groups compared with Control. Replacement of BC with SRS in goat rations increased milk levels of conjugated linoleic acid and unsaturated fatty acids compared with Control. It was concluded that replacing 50% of Egyptian berseem clover with SRS in goat rations improved their productive performance without marked effects on metabolic indicators health.