• The Plant Pathology Journal
• /
• v.19 no.3
• /
• pp.133-137
• /
• 2003

Seedborne fungus Bipolaris spicifera, which has not been previously reported in Korea, was detected from import-ed grass seeds in the country. The most frequently detected fungi from the seeds were Fusarium species, Ulocladium atrum, B. spicifera, Alternaria, and Cuvularia lunata among 17 different seed samples of the family Gramineae. Detection frequencies of B. spicifera were 11,8,5% in Bermuda grass, tall fescue, and mixed lawn grass imported from USA, respectively, and 9％ in mixed lawn grass imported from Italy. This suggests that important seedborne pathogen could be spread between countries through seed sources. The pathogen was seed-transmitted causing damping-off of Bermuda grass seedlings and showed strong pathogenicity to vice, corn, Bermuda grass, sorghum, and tall fescue. However, it did not infect wheat and blue grass.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.2
• /
• pp.206-215
• /
• 2009

This experiment was carried out to evaluate performance and carcass characteristics of 40 crossbred young bulls ($Zebu{\times}European$) finished in a feedlot under two roughage sources (Bermuda grass hay or sorghum silage) with or without the addition of yeast (Saccharomyces cerevisae). The bulls were 20 months old, their initial average weight was 356 kg and they were allocated into four groups of ten animals. The experimental diets were Bermuda grass, Bermuda grass+yeast, sorghum silage and sorghum silage +yeast. Animal performance and carcass characteristics were not influenced by roughage source or yeast addition. The average daily weight gain was 1.50 kg, dry matter intake (DMI) was 11.1 kg/d, DMI as percentage of liveweight was 2.60% and feed dry matter conversion was 7.70. The mean dressing percentage was 52.0% and hot carcass weight was 268 kg. Carcass conformation was classified between good-minus to good. Carcass length (137 cm), leg length (72.9 cm) and cushion thickness (26.6 cm) were not influenced by treatments. The average fat thickness was 3.80 mm and the Longissimus muscle area was 66.9 $cm^{2}$. The classification of color, texture and marbling were slightly dark red to red, fine and slight-minus to light-typical, respectively. The mean percentage of bone, muscle and fat in the carcass was 15.5%, 62.3% and 22.5%, respectively. Yeast addition increased ${\gamma}$-linolenic fatty acid (0.15 vs. 0.11%) deposition. Bermuda grass hay increased deposition of ${\alpha}$-linolenic (0.49 vs. 0.41%), arachidonic (2.30 vs. 1.57%), eicosapentaenoic (0.41 vs. 0.29%), docosapentaenoic (0.80 vs. 0.62%), docosahexaenoic (0.11 vs. 0.06%) and n-3 fatty acids, and reduced n-6: n-3 ratio in meat, when compared to sorghum silage treatments. The treatments had no effect on saturated fatty acids (49.5%), polyunsaturated fatty acids (11.8%), n-6 fatty acids (9.87%), n-3 (1.61%) and PUFA:SFA ratio (0.24). Monounsaturated fatty acid levels were higher on sorghum silage (40.7 vs. 37.7%). The addition of yeast caused higher n-6: n-3 ratio (7.28 vs. 5.70) than treatments without yeast.

• Analytical Science and Technology
• /
• v.9 no.1
• /
• pp.107-111
• /
• 1996

A phytotoxic compound was isolated from a culture of Bipolaris cynodontis, a fungus pathogenic to Bermuda grass. The structure was determined by spectroscopic analyses including 2D NMR experiments, to be sesquiterpene having a 9-carbon unit side chain. The compound inhibits the root growth of the seedlings of Italian ryegrass and rice plant, the host plant of the B. cynodontis, by about 100% at 100ppm, and it is suggested that this may play an important role in the expression of the disease symptom.

• Bulletin of the Korean Chemical Society
• /
• v.28 no.4
• /
• pp.613-618
• /
• 2007

Pollens are known to be an allergen. They penetrate human respiratory system, triggering a type of seasonal allergic rhinitis called pollen allergy (hey fever). The purpose of this study is to test two field-flow fractionation (FFF) techniques, gravitational FFF (GrFFF) and sedimentation FFF (SdFFF), for their applicability to sizecharacterization of micron-sized pollens. Both GrFFF and SdFFF are elution techniques, providing sequential elution of particles based on size. They allow the size distribution as well as the mean size of the sample to be determined from the elution time. In this study, GrFFF and SdFFF were used to determine the size distribution of Paper Mulberry and Bermuda Grass pollens. For the Paper Mulberry pollen, the mean size obtained by GrFFF is 12.7 μm, and agrees rather well with the OM data with the relative error of 8.0%. For the Bermuda Grass pollen, the mean size obtained by GrFFF is 32.6 μm with the relative error of 12.3%. The mean sizes determined by SdFFF are 12.4 (relative error = 10.1%) and 27.1 μm (relative error = 5.2%) for the Paper Mulberry and the Bermuda Grass pollen, respectively. Although SdFFF tends to yield more accurate size distribution due to lower band broadening under the field strength higher than 1 G, the sizes determined by GrFFF were not significantly different from those by SdFFF.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.25 no.3
• /
• pp.169-176
• /
• 2005

To evaluate the possibility of predicting a specific nutrient component, which nay be difficult in analysis, from a component of nutrient relatively easy to analyze, six grass roughage sources (bermuda grass hay, klein grass hay, oat hay, orchard grass straw, tall fescue straw and timothy hay) mainly used in Korea were analyzed for the heat-stable $\alpha-amylase$ treated neutral detergent fiber (aNDF), acid detergent fiber (ADF), acid detergent lignin (ADL), neutral detergent insoluble crude protein (NDICP) and acid detergent insoluble crude protein (ADICP) as well as proximate analytical components, and correlation coefficients between nutrient components were calculated. Some chemical components appeared to have significant correlation (p<0.05) through Pearson's correlation coefficients (i.e. between crude protein (CP) and NDICP in oat hay, orchard grass straw and tall fescue, between CP and ADICP in oat hay and timothy hay, between crude fiber (CF) and aNDF in klein grass hay, tall fescue straw and timothy hay, between CF and ADF in oat hay, tall fescue straw and timothy hay, and between CF and ADL in orchard pass straw and timothy hay). It is therefore suggested that it would be worthy to predict a specific nutrient component, which may be difficult in analysis such as aNDF, ADF, ADL, NDICP and ADICP, from a component of nutrient relatively easy to analyze such as CP and CF. However, it would be needed to improve the correlation coefficients, more samples should be measured.

• Clinical and Experimental Pediatrics
• /
• v.55 no.9
• /
• pp.322-329
• /
• 2012

Purpose: Aeroallergens are important causative factors of allergic diseases. Previous studies on aeroallergen sensitization rates investigated patients groups that had visited pediatric allergy clinics. In contrast, we investigated sensitization rates in a general population group of elementary school to teenage students in Incheon, Jeju, and Ulsan. Methods: After obtaining parental consent, skin-prick tests were performed on 5,094 students between March and June 2010. Elementary school students were tested for 18 common aeroallergens, whereas middle and high school students were tested for 25 allergens. The 25 allergens included Dermatophagoides pteronyssinus, Dermatophagoides farinae, pollen (birch, alder, oak, Japanese cedar, pine, willow, elm, maple, Bermuda grass, timothy grass, rye grass, orchard grass, meadow grass, vernal grass, mugwort, Japanese hop, fat hen, ragweed, and plantain), and mold (Penicillatum, Aspergillus, Cladosporium, and Alternaria). Results: The sensitization rates in descending order were 25.79% (D. pteronyssinus ), 18.66% (D. farinae ), 6.20% (mugwort), and 4.07% (willow) in Incheon; 33.35% (D. pteronyssinus ), 24.78% (D. farinae), 15.36% (Japanese cedar), and 7.33% (Alternaria) in Jeju; and 32.79% (D. pteronyssinus), 30.27% (D. farinae), 10.13% (alder), and 8.68% (birch) in Ulsan. The dust mite allergen showed the highest sensitization rate among the 3 regions. The sensitization rate of tree pollen was the highest in Ulsan, whereas that of Alternaria was the highest in Jeju. The ragweed sensitization rates were 0.99% in Incheon, 1.07% in Jeju, and 0.81% in Ulsan. Conclusion: The differences in sensitization rates were because of different regional environmental conditions and distinct surrounding biological species. Hence, subsequent nationwide studies are required.

• Asian-Australasian Journal of Animal Sciences
• /
• v.5 no.2
• /
• pp.279-283
• /
• 1992

Eighteen wether lambs averaging 32 kg were used to determine the effects of dietary silica, added as silicic acid, on mineral metabolism. Lambs were fed 1200 g daily of a coastal Bermuda grass based diet supplemented with either 0, .5 or 1.5% silicic acid. A 7-d total collection of urine and feces was conducted after lambs had adjusted to the dietary treatments for 19 days. Urinary excretion of silica was higher (p<.01) in lambs fed added silicic acid. Ruminal soluble concentrations of manganese tended to be lower (p<.10) and apparent absorption and retention of manganese were lower (p<.05) in lambs supplemented with silicic acid compared to control lambs. Apparent absorption and retention of calcium were slightly lower (p<.10) in silicic acid fed lambs. No differences in urinary between lambs fed .5 and those given 1.5% silicic acid. Phosphorus, magnesium, iron, zinc and copper absorption and retention were not affected by treatment.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.1
• /
• pp.53-62
• /
• 2004

The aim of this study was to predict the energy value and dynamic degradation of roughage in Taiwan using the $Daisy^{(R)}$. in vitro fermentation method to provide information on one of the very important nutrients for ration formulation. The second objective was to study the effects of Aspergillus oryzae (AFE) inclusion on nutrient utilization. Three ruminal fistulated dry dairy cows were used for rumen fluid and fifteen conventional forages used in dairy cattle were collected around this island. The degradability of these feedstuffs with and without AFE ($Amaferm^{(R)}$.) treatment was measured using the $Daisy^{(R)}$. in vitro method. The roughage energy values, including TDN and NEL, were calculated according to Robinson (2000). Results from the 30 h in vitro neutral detergent fiber (NDF) degradability and predicted energy evaluations showed that alfalfa (among the forages) contained the highest degradability and energy values, Bermuda straw having the lowest. Peanut vines and corn silage contained higher energy values and the lowest value found in Pangola and Napier grasses among the locally produced forages. Pangola and Napier grasses had lower values than most imported forages except Bermuda straw. Among the by-products, wheat middling contained the highest NDF degradability, while rice bran contained the richest energy value due to its high oil content. From the dynamic dry matter (DM), organic matter (OM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) degradation, corn silage contained the highest effective degradation among the local forages; wheat middling (among the by-products) degraded the fastest in DM, OM, ADF and NDF and showed the highest effective degradability. AFE inclusion was inconsistent among the forages. Alfalfa hay showed significantly increased 30 h NDF degradability and energy values, Pangola hay, Napier grass and brewer's grains showed decreased degradability and energy values. AFE inclusion increased the DM, OM and NDF degradation rate in most forage, but only increased the DM degradation rate in sorghum distiller's grains, the OM degradation rate in bean curd pomace and the NDF and ADF degradation rates in soy pomace (among the by-products).

• Water Engineering Research
• /
• v.5 no.2
• /
• pp.55-68
• /
• 2004

The GLEAMS (Groundwater Loading Effects of Agricultural Management System, version 3.0) water quality model was used to predict hydrology and water quality and to evaluate the effects of soil types from a cattle-grazed pasture field of Bermuda-Rye grass rotation with poultry litter application as a fertilizer in North Alabama. The model was applied and evaluated by using four years (1999-2002) of field-measured data to compare the simulated results for the 2.71- ha Summerford watershed. $R^2$ values between observed and simulated runoff, sediment yields, TN, and TP were 0.91, 0.86, 0.95, and 0.69, respectively. EI (Efficiency Index) of these parameters were 0.86, 0.67, 0.70, and 0.48, respectively. The statistical parameters indicated that GLEAMS provided a reasonable estimation of the runoff, sediment yield, and nutrient losses at the studied watershed. The soil infiltration rates were compared with the rainfall events. Only high intensity rainfall events generated runoff from the watershed. The measured and predicted infiltration rates were higher during dry soil conditions than wet soil conditions. The ratio of runoff to precipitation was ranging from 2.2% to 8.8% with average of 4.3%. This shows that the project site had high infiltration and evapotranspiration which generated the low runoff. The ratio of runoff to precipitation according to soil types by the GLEAMS model appeared that Sa (Sequatchie fine sandy loam) soil type was higher and Wc (Waynesboro fine sandy loam, severely eroded rolling phase) soil type relatively lower than the weighted average of the soil types in the watershed. The model under-predicted runoff, sediment yields, TN, and TP in Wb (Waynesboro fine sandy loam, eroded undulating phase) and Wc soil types. General tendency of the predicted data was similar for all soil types. The model predicted the highest runoff in Sa soil type by 105% of the weighted average and the lowest runoff in Wc soil type by 87% of the weighted average

• Analytical Science and Technology
• /
• v.9 no.1
• /
• pp.112-117
• /
• 1996

Three active compounds were isolated from the culture of a plant pathogenic fungus, Bipolaris cynodontis. The structure elucidation of these compounds was accomplished by 2D NMR techniques, such as $^1H-^1H$ and $^{13}C-^1H$ COSY, COLOC, HMBC and rotating frame NOE(ROESY). Compounds were found as derivatives of cochlioquinone and cochlioquinol that were previously isolated as phytotoxins from B. bicolor and B. cynodontis, respectively. The compounds showed phytotxicity against Italian ryegrass, one of the host plants of B. cynodontis.