• International Journal of Oral Biology
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• 제36권1호
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• pp.31-35
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• 2011

Teeth develop via a reciprocal induction between the ectomesenchyme originating from the neural crest and the ectodermal epithelium. During complete formation of the tooth morphology and structure, many cells proliferate, differentiate, and can be replaced with other structures. Apoptosis is a type of genetically-controlled cell death and a biological process arising at the cellular level during development. To determine if apoptosis is an effective mechanism for eliminating cells during tooth development, this process was examined in the rat mandible including the developing molar teeth using the transferase-mediated dUTP-biotin nick labeling (TUNEL) method. The tooth germ of the mandibular first molar in the postnatal rat showed a variety of morphological appearances from the bell stage to the crown stage. Strong TUNEL-positive reactivity was observed in the ameloblasts and cells of the stellate reticulum. Odontoblasts near the prospective cusp area also showed a TUNEL positive reaction and several cells in the dental papilla, which are the forming pulp, were also stained intensively in this assay. Our results thus show that apoptosis may take place not only in epithelial-derived dental organs but also in the mesenchyme-derived dental papilla. Hence, apoptosis may be an essential biological process in tooth development.

• 한국미생물·생명공학회지
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• 제19권1호
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• pp.37-44
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• 1991

진핵세포 유전자의 기초대사발현의 조절계를 밝히기 위한 일환으로, 효모의 histidine생합성계 효소의 구조유전자 HIS5를 이용하였다. HIS5 유전자는 충분한 아미노산 조건하에서는 발현이 억제되어 비교적 높은 기초발현만을 하나, 어떤 아미노산이 결핍되면 탈억제되어 높은 발현량을 보이며 탈억제는 cis의 작용인자인 promoter상의 5'-TGACTC-3' 및 trans 작용인자 GCN4와 GCD17 GCN2등이 관여한다. trans 작용인자들에 의한 HIS5 유전자의 발현량의 변화를 간단하게 측정하기 위하여, HIS5 promoter와 repressible acid phoshates(APase)의 구조유전자중 promoter를 제거한 DNA단편을 연결시켜 HIS5-PHO5 융합유전자를 이용하였다. gcn2 및 gcn4 변이주의 APase 활성은 야생주와 비교하여 3내지 4배 낮았으며, gcn2변이주와 gcn2 gcn4 이중변이주의 APase 활성은 유사하였다.

• 한국식품조리과학회지
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• 제15권3호
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• pp.272-287
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• 1999

Elementary schools students'degree of satisfaction on school food service and food preference were surveyed from 352 sixth-grade students in Kwangju and Chonnam area from June 22 to July 3 to enhance the quality of foodservice. The degree of satisfaction was high with cooked rice and soup in terms of temperature and serving state. There were significant differences in the degree of satisfaction depending upon the types of foodservice (p<0.001). There was significant differences in degree of satisfaction for side dishes depending upon the temperature (p<0.05), kinds (p<0.01) and amount (p<0.001). The degree of satisfaction with Kimchi was generally high in the serving state (p<0.05) and the amounts (p<0.01). The degree of satisfaction with desserts were high in the amount (p<0.001). The amount of leftover was high with soup, side dishes, kimchi, cooked rice, and desserts in order. The improvements made on children's behavior after the implementation of the school foodsevice were the adaptation to balanced diet and spending less money on sweets. The factors of influencing the school foodservice and the children's food intake were the education in school (teachers and dietitians) and the health status of their bodies (p<0.001). Children's favorite foods were sweet and sour pork, pork cutlets, oranges, watermelons, and strawberries in order. The least preferred foods were garlic seasoned with soy sauce, raw vegetables of broad bell-flowers, crown daisy greens, and steamed peppers. Children's favorite food types were fried food, desserts, one course meal, and bread. The least preferred types were pickled food, green and seasoned vegetables, cooked rice, and hard-boiled food in order. Generally, the children in the rural area favored most foods compared with those from urban area.

• Molecules and Cells
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• 제40권10호
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• pp.714-730
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• 2017

Pre-mRNA splicing further increases protein diversity acquired through evolution. The underlying driving forces for this phenomenon are unknown, especially in terms of gene expression. A rice alternatively spliced transcript detection microarray (ASDM) and RNA sequencing (RNA-Seq) were applied to differentiate the transcriptome of 4 representative organs of Oryza sativa L. cv. Ilmi: leaves, roots, 1-cm-stage panicles and young seeds at 21 days after pollination. Comparison of data obtained by microarray and RNA-Seq showed a bell-shaped distribution and a co-lineation for highly expressed genes. Transcripts were classified according to the degree of organ enrichment using a coefficient value (CV, the ratio of the standard deviation to the mean values): highly variable (CVI), variable (CVII), and constitutive (CVIII) groups. A higher index of the portion of loci with alternatively spliced transcripts in a group (IAST) value was observed for the constitutive group. Genes of the highly variable group showed the characteristics of the examined organs, and alternatively spliced transcripts tended to exhibit the same organ specificity or less organ preferences, with avoidance of 'organ distinctness'. In addition, within a locus, a tendency of higher expression was found for transcripts with a longer coding sequence (CDS), and a spliced intron was the most commonly found type of alternative splicing for an extended CDS. Thus, pre-mRNA splicing might have evolved to retain maximum functionality in terms of organ preference and multiplicity.

• 한국광물학회지
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• 제19권3호
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• pp.189-195
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• 2006

황철석에 대한 압축성 연구를 다이아몬드 앤빌 기기 및 방사광을 이용하고 에너지분산 X-선회절법을 적용하여 상온에서 시행하였다. 황철석의 체적탄성률은 실험조건과 실험기기에 따라 매우 큰 변이를 보이고 있는데 이는 압력전달매체의 특성에 따른 것으로 알려져 있다. $FeS_{2}#1$(황철석+NaCl)과 $FeS_{2}#2$(황철석+MgO)에 대해 압력전달 매체로 각각 NaCl과 유체를 이용하여 체적탄성률 138.9 GPa와 198.2 GPa를 각각 얻었다. 이러한 값은 시료방의 압력상태에 따른 예측 결과치에 대치되는 것으로 그 원인은 압력전달액체의 상변이에 따른 것으로 추정되며 이외에 고상검출기의 차이, E*d값의 차이 등도 영향을 미친 것으로 판단된다. 모든 실험은 포항가속기연구소 1B2 빔라인에서 시행되었다.

• 한국축산시설환경학회지
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• 제19권1호
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• pp.33-38
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• 2013

We conducted investigations of the information and welfare of broiler chickens in Korea. The livestock housing of all broiler chicken farms were windowless type, bell feeder and nipple waterer were used, and stocking density was relatively higher (ranging from 18.2 $birds/m^2$ to 24.2 $birds/m^2$) than RSPCA's welfare standards for chicken hens (less than 19 $birds/m^2$). The ratios of sandwich panel, urethane form, slate, and steel plate in roofing materials were 61%, 21%, 13%, and 5%, respectively. The ratios of sandwich panel, urethane form, brick, and steel plate in wall materials were 61%, 21%, 13%, and 5%, respectively. The ratios of soil and concrete in flooring materials were 10% and 90%. The mist spray, fan, and cooling pad in cooling facilities were 42%, 32%, and 26%, respectively. Thus we believe that present data contribute to develop the animal welfare certification for broiler chickens and to improve animal welfare in Korea.

• 한국광물학회지
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• 제23권3호
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• pp.267-272
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• 2010

${\alpha}$-크리스토발라이트 구조의 $GaPO_4$에 대한 고압 x-선 회절 실험을 상온에서 8.9 GPa까지 시행하였다. 정방정계에 속하는 출발결정구조는 1 GPa 이하의 압력에서 회절피크가 분리되는 변화를 보이는데, 아마도 망상구조를 이루고 있는 사면체의 방향성이 압력에 의해 흐트러지면서 상-I'로 상변이가 유도된 것 같다. 압력을 증가시키면, 상-I'는 2 GPa와 3 GPa 사이에서 정방정계에 속하는 고압상(상-III)으로 상변이를 하고 있다. 이 결과는 최근 동일한 시료를 이용하여 얻은 고압실험결과(Ming et al., 2007)와 일치하지 않는다. 최고압력에 도달한 다음, 압력을 대기압으로 내리면 상-III이 그대로 유지되는 것으로 나타났다.

• 한국추진공학회지
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• 제19권3호
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• pp.47-53
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• 2015

이론성능해석을 통하여 메탄/산소 이원추진제 소형로켓엔진의 형상설계에 필요한 설계인자들을 도출하였다. 로켓엔진의 이론성능 분석을 위해 CEA를 이용하였고, 추력실 내부의 화학반응을 화학평형 상태로 가정하여 엔진의 형상설계에 필요한 최적의 추진제 혼합비, 특성길이, 최적 팽창비 등을 도출하였다. 인젝터의 경우, 미립화 성능이 우수하고, 다른 인젝터 형상에 비해 연소효율이 높은 스월 동축 인젝터를 설계하였다. 노즐형상은 80%의 길이를 갖는 벨형 노즐을 설계하였고, 추력실 내부압력 1.72 MPa, 총 추진제 질량유량 0.18 kg/s, O/F ratio 2.7일 때의 지상연소시험용 로켓엔진의 형상설계 결과를 제시한다.

• 지질공학
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• 제30권3호
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• pp.347-378
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• 2020

SWAT-MODFLOW의 월평균 수문성분 자료를 바탕으로 지하수 함양관련 토양층의 측방유출, 침루량을 산정하는 기본자료인 수문응답단위(HRU)와 이를 구성하는 토지피복, 토지이용, 토양통에서 영향이 큰 지하수 함양량 인자를 분석하였다. 봄, 가을별 bare soil line(BSL)을 통해 PVI를 분석하고 기존의 NDVI, NDTI, NDRI와 함께 함양량 분포와 토양-식생관련 지수의 빈도분석과 회귀분석을 수행하였다. 그 결과 비정규분포 양상을 보이는 NDVI와 NDTI에서 매우 유의한 양의 상관성이 확인되었다. 연구지역의 bare soil line 기울기는 약 1.092~1.343, 절편은 약 -0.004~-0.015이며 상대적으로 월평균강우량이 우세한 5월에는 Red밴드가 다른 시기에 비해 약 3~4배 증가하고 2012년 Singhal and Goyal의 결과와 같이 포물선 형태의 유일한 1개의 변곡점이 발생하는 것을 확인하였다. 따라서 강우를 고려하여 지하수 함양과 토양-식생관련 지수에 대한 다양한 경우의 상관성 분석이 수행된다면, 위성영상자료를 통해 토양-식생에 따른 함양량 변화도 추정할 수 있을 것으로 기대된다.

• 대한간호학회지
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• 제26권3호
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• pp.709-729
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• 1996

This study was done to analyze the trends of research on coping in Korea, to suggest future direction, for research on coping, and ultimately to contribute to an increase in explanation of adaptation. This article reviewed 79 nursing research papers on coping done since 1978 by examining them according to the period of publication or presentation, research design, type of subjects, measurement instruments, research for a degree or not, range of reliability, and association of coping and related variables. The results are as follows : The number of studies on coping increased rapidly from the mid-1980's and decreased slowly from the mid-1990's. The maority of the studies were surveys, comparative studies, or correlational studies. The subects of the 46 studies were healthy people, while those in the remaining studies were patients with a variety of illnesses. Thirth-eight studies on coping were done for master's thesis, three for dissertion, and 38 were not degrees. The Bell and Jalowiec coping scales have not been used since the early 1990's. In contrast, Lazarus and Folkman's W.C.C.L. has been used increasingly since that time. The reliabilities of the coping scale were reported in 37 cases and the Cronbach's alpha coefficients were .71 to .86. All subjects reported using more problem-oriented coping than emotion-oriented coping in short-term or emotion-oriented coping and healthy groups did more long-term coping. It was difficult to describe consistently the relationship between stress and coping according to the type of coping scale or research subjects, but generally moderate relationships were found. This was due to instrumental problems and no consideration of situational context. The subject group who used more short-term coping and less long-term coping reported poorer mental status, and higher scores in burnout and state anxiey than others. That is, the relationship between stress and adaptation increased the power of explanation with intervening the mediating effect of coping. The association of locus of control, mastery, social support, and self-concept with coping showed positive relationships : those of uncertainty and severity in illness with coping showed negativerelationships ; those of state anxiety and depression with short-term coping were positive, and those of self-esteem with long-term coping or problem-oriented coping were negative. There were significant differences in the scores of types of coping according to religion, level of education, and socio-economic status. That is, Presbyterians and Catholics, those with higher education levels and higher socio-economic status used more long-term or problem-oriented coping. On the basis of the above findings the following recommendations are made : 1. There is a need to test the mediating effect of coping variable in order to clarify the concept. 2. Longitudinal studies are needed to determine the patterns of change in coping strategies when stressful events are encountered. 3. It's necessary to develop a reliable and variable measurement tool for coping. 4. There is a need to identify subscales of coping to increase explanation of variance 5. It's necessary to consider personal, situational, and antecedent variables : the characteristics of subject populations, the natures of illness and treatment situations. 6. The power of explanation of studies designed to identify the stress-adaptation process should be increased using the combination model of process-oriented coping and cognitive-structural model.