• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.551-559
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• 2005

The recent rapid increase in genomic data related to many microorganisms and the development of computational tools to accurately analyze large amounts of data have enabled us to design several kinds of simulation approaches for the complex behaviors of cells. Among these approaches, dFBA (dynamic flux balance analysis), which utilizes FBA, differential equations, and regulatory events, has correctly predicted cellular behaviors under given environmental conditions. However, until now, dFBA has centered on substrate concentration, cell growth, and gene on/off, but a detailed hierarchical structure of a regulatory network has not been taken into account. The use of Boolean rules for regulatory events in dFBA has limited the representation of interactions between specific regulatory proteins and genes and the whole transcriptional regulation mechanism with environmental change. In this paper, we adopted the operon as the basic structure, constructed a hierarchical structure for a regulatory network with defined fundamental symbols, and introduced a weight between symbols in order to solve the above problems. Finally, the total control mechanism of regulatory elements (operons, genes, effectors, etc.) with time was simulated through the linkage of dFBA with regulatory network modeling. The lac operon, trp operon, and tna operon in the central metabolic network of E. coli were chosen as the basic models for control patterns. The suggested modeling method in this study can be adopted as a basic framework to describe other transcriptional regulations, and provide biologists and engineers with useful information on transcriptional regulation mechanisms under extracellular environmental change.

• Coupled systems mechanics
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• 제7권3호
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• pp.297-319
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• 2018

In this paper, the vibro-acoustic behaviors of vibrational cylindrical shells are investigated by using structural intensity approach. The reducing interior noise method for vibrating cylindrical shells is proposed by altering and redistributing the structural intensity through changing the damping property of the structure. The concept of proposed novel method is based on the properties of structural intensity distribution on cylindrical shells under different load and damping conditions, which can reflects power flow in the structures. In the study, the modal formulas of structural intensity are developed for the steady state vibration of cylindrical shell structures. The detailed formulas of structural intensity are derived by substituting modal quantities, in which the effect of main parameters such as weight coefficients and distribution functions on structure intensity are analyzed and discussed. Numerical simulations are first carried out based on the structural intensity analytical solutions of modal formulas. Through simulating the coupling vibration and acoustical radiation problems of cylindrical shell, the relationship between vibro-acoustic and structural intensity distribution is derived. We find that for cylindrical shell, by properly arranging damping conditions, the structural intensity can be efficiently changed and further the noise property can be improved. The proposed methodology has important implications and potential applications in the vibration and noise control of fuselage structure.

• 약학회지
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• 제51권3호
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• pp.219-227
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• 2007

Ginsenoside Rgl (Rgl), the pharmacologically active constituent of ginseng (Panax ginseng C.A. Meyer), has a variety of biological activities. The present study was undertaken to evaluate a possibility of Rgl whether it can be used in treatment or prevention of stress disorders. Animals were stressed by immobilization for 2 hours or electroshocks for 20 minutes. The normal group was not exposed to any stress. Rgl was subcutaneously injected as dosages of 5 and 10 mg/kg and red ginseng (RG) was orally administered 200 mg/kg as the positive control. Animals were given supplements for 5 days without stress, and then were given supplements for 5 days with stress. We recorded stress-related behavioral changes of experimental animals using the Etho-vision system. Weight of adrenal gland and levels of corticosterone in plasma were measured and stress related behaviors (smelling, grooming, face washing, rearing) were observed. Rgl didn't make significant behavioral changes in total open field and elevated plus maze test. Rgl did not influence on behavioral changes induced by electroshock stress. Whereas, 10 mg/kg of Rgl alleviated the increment of the freezing and face washing time and the decrement of the smelling and rearing time induced by restraint stress. The administration of Rgl 10 mg/kg has significantly increased the endurance time on rotating rod and swimming pool tests compared to the control group. These results indicate that Rgl can alleviate the damage induced by physical stress. This result suggests that Rgl may bea new candidate for treating stress related disorder.

• Asian-Australasian Journal of Animal Sciences
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• 제31권3호
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• pp.379-385
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• 2018

Objective: The study aimed to evaluate sugarcane bagasse as roughage in lactating cow on feed intake, digestibility, ingestive behavior, milk production and composition, and microbial protein synthesis. Methods: Ten Girolando cows at initial body weight of $450{\pm}25.6kg$ and at $143.7{\pm}30.7days$ in milk were assigned in two $5{\times}5$ Latin square designs. Five 21-day experimental periods were adopted ($1^{\circ}$ to 14-day: diets adaptation period; $15^{\circ}$ to 21-day: data collection and sampling period). The diets consisted of four different levels of sugarcane bagasse (45%, 50%, 55%, and 60%) and a control diet, commonly adopted in the region, based on spineless cactus (25% sugarcane bagasse), formulated to meet 12 kg/d milk yield. Results: The dry matter (DM), organic matter (OM), and total digestible nutrients intakes and DM and OM digestibilities observed for 45% and 50% bagasse inclusion were similar to control diet, while that 55% and 60% bagasse inclusion were lower. Cows fed control diet, and bagasse diets of 45%, and 50% levels had the nutritional requirements attended, that guaranteed 12 kg/d of milk yield. The crude protein intake and digestibility of cows fed 45%, 50%, and 55% of bagasse inclusion were similar to control diet. The neutral detergent fiber (NDF) intake and digestibility differ for all bagasse diets related to control diet, while the non-fiber carbohydrates intake and digestibility for cows fed 45% of bagasse were similar for control diet. The intakes and digestibilities of nutrients decreased linearly in function of bagasse inclusion; NDF and indigestible NDF intakes did not vary. The ruminating time, feeding and rumination efficiency, microbial protein synthesis and milk yield decreased linearly with sugarcane bagasse inclusion. Conclusion: Sugarcane bagasse decreases milk production; however, its inclusion level in between 45% to 50% associated to concentrate could replace diets based on spineless cactus for crossbred dairy cow's producing 12 kg/d of milk.

• 한국식품영양학회지
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• 제29권3호
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• pp.392-403
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• 2016

The purpose of this study was to investigate the relations between HbA1c level and health risk factor. For analysis, 330 elderly women aged 65 years and over were recruited in Jeonla province, Korea. The subjects were classified into two groups according to their HbA1c level; the diabetic group (HbA1c level${\geq}6.5%$, n=68, 20.6%) and control group (HbA1c level<6.5%, n=262, 79.4%). Demographic characteristics were collected, as well as information on physical measurements, blood tests for biochemical indicators, a health status and health-related life style, dietary behavior, favorite food groups, consumption frequency of food groups and nutrient intake. Educational level of the diabetic group was significantly lower than that of the control group. The height of the diabetic group was shorter, and percentage fat of the diabetic group was higher than those of the control group. In the diabetic group, the higher concentrations of creatinine and activity of alkaline phosphatase were found to be significantly unfavorable factors. Therefore, the diabetic group was assumed to be at risk of decreased liver and renal function. The self-rated health level of the diabetic group has a declining tendency, while the medicine intake was significantly higher than that of the control group, but there are no significant differences in visiting frequency of hospital and community health center. The distribution of physical activity showed a significant difference between the groups. Although there are no significant differences in nutrient intake, the diabetic group had relatively inferior nutrient intake of diet, especially vitamin C, as contrasted with the control group. In conclusion, the weight control and increasing physical activity may be effective in the prevention of the diabetes and continuous education and intervention by specialized nutritionists will be needed for diabetic patients. These results could be useful to plan effective strategies to increase the health-life expectancy and the prevention of the diabetes of Korean elderly people living in rural areas.

• Asian-Australasian Journal of Animal Sciences
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• 제32권8호
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• pp.1145-1152
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• 2019

Objective: Mulberry (Morus alba L.) is a cultivated shrub grown widely in the sub-tropical and tropical areas. It has been shown that mulberry leaf contains high levels of protein while having polyphenols as phytonutrients. Therefore, it is important to conduct an experiment to assess potential toxic level from mulberry on behavior, blood hematological and coagulation parameter using Sprague-Dawley (SD) rats. Methods: Both male and female SD rats were given an intragastric administration of respective treatments of mulberry leaf intakes (control, low and high levels). Parameters of feed intake, hematological and coagulation of blood parameters, as well as liveweight changes were taken during the 7 d of adaptation, 28 d of treatment exposure, and 14 d of recovery periods, respectively. All treatment data were statistically analyzed using analysis of variance of SPSS17.0 for Windows Statistical Software following the Randomized complete block design with sex as a block. Results: Most of the parameters of the physical symptoms of the SD rats, were not significantly different (p>0.05) when compared with that of the control group. Those which remain unchanged in each dose group were, body weight (BW) gain, feed intake, the hematology and coagulation indexes. Although, there were a few individual indicators that were abnormal, but the overall physiological appearance of the rats were normal. Conclusion: Results under this experiment revealed that most hematological and coagulation parameters of the SD rats in both male and female were normal, although the weight gain of female rats in high-dose group was significantly reduced than those of the male rats. Under this study, the use of mulberry leaf up to 2 g/kg BW did not result in abnormal phenomenon in the SD rats. These findings would offer useful information for further in vivo feeding trials in animals to extensively use of mulberry leaf to improve animal production, particularly in P.R. China.

• 폴리머
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• 제35권1호
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• pp.77-86
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• 2011

굳은 특성을 가진 다리로 연결된 이핵 CGC 6개의 공중합특성을 조사하였다. 6개의 화합물 중에서 3개는 길이가 다른 para-phenyl (Catalyst 1), para-xylyl (Cata1yst 2), para-diethylene phenyl (Catalyst 6) 다리를 가진 화합물이며, 나머지 3개는 다리리간드는 para-xylyl 다리 기본구조를 가졌으나 치환체가 isopropyl (Catalyst 3), n-hexyl (Cataylst 4), n-octyl (Catalyst 5)인 화합물이었다. 이핵메탈로센 6가지와 Dow 촉매를 사용하여 에틸렌과 스티렌을 공중합시켜 다리리간드의 특성변화가 촉매의 중합특성과 이로부터 생성되는 공중합체의 특성에 미치는 영향을 조사하였다. 실험 결과 다리리간드의 길이가 증가함에 따라 촉매의 중합활성이 4배까지 향상되었으며 이로부터 생성되는 공중합체의 분자량도 증가하였다. 또한 para-xylyl 리간드의 치환체가 isopropyl에서 n-hexyl 및 n-octyl로 변함에 따라 중합활성은 증가하였으나 이로부터 생성되는 분자량은 감소하였다. 본 연구 결과는 촉매구조 변화에 의한 고분자 미세구조 조절이라는 고분자 합성의 가장 어려운 부분이 이핵메탈로센을 활용하여 어느 정도 현실화될 수 있음을 보여주는 결과이다.

• 한국콘텐츠학회논문지
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• 제14권10호
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• pp.198-209
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• 2014

당뇨병은 유병률과 합병증으로 인한 사망률이 지속적으로 증가하는 질병이다. 본 연구는 성인의 당뇨병 유병률 영향요인을 확인함으로써 당뇨병 유병률의 감소를 위한 중재전략 개발의 기초자료를 제공하고자 시도되었다. 연구대상자는 19세 이상 성인으로 2012년 국민건강영양조사 원시자료를 활용하여 분석하였으며, 5995명의 자료가 분석에 사용되었다. 수집된 자료는 SPSS 18.0 프로그램의 복합표본 분석 모듈을 사용하여 가중치를 부여한 후 분석하였다. 연구결과 19세 이상 성인의 당뇨병 유병률은 10.5%였고, 당뇨병 유병 오즈비는 학력에서 대졸 이상에 비해 중졸은 2.51배, 주관적 건강상태가 매우 좋은 경우에 비해 매우 나쁜 경우 4.77배, 비만인 경우 1.44배, 고혈압을 진단받은 경우 2.57배, 고지혈증을 진단받은 경우 2.63배, 체중조절을 하지 않는 경우 1.31배, 식이요법을 하는 경우 2.75배로 높았다. 본 연구는 당뇨병 유병률을 예측하는 변수로 교육수준, 주관적 건강상태, 비만 유병 여부, 고혈압 의사진단 여부, 고지혈증 의사진단 여부, 체중조절 여부, 식이요법 여부를 밝힘으로써 당뇨병 유병률을 감소시키기 위한 간호중재와 연구방향을 제시하였다.

• 한국심리학회지 : 코칭
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• 제5권1호
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• pp.1-32
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• 2021

본 연구의 목적은 습관행동 변화과정 모형(탁진국, 권혜란, 김혜영 등, 2019)을 기반으로 하여, 식습관 개선 성공 요인(최경화, 김혜영, 한석빈, 탁진국, 2020)과 선행 연구에서 습관 개선에 효과를 나타낸 방법들을 토대로 식습관 개선을 위한 온라인 코칭 프로그램을 개발하여 실시하고, 그 효과를 검증하는 데 있다. 식습관 개선을 희망하며 수도권에 거주 중인 19세 이상 성인 중 전화인터뷰를 통해 선정된 20명을 실험 및 통제집단으로 나누어 일대일 코칭을 진행하였다. 최종분석에는 17명의 자료가 사용되었으며, 프로그램 사전과 사후에 체중, 식습관, 식이행동, 식이효능감, 자기조절, 삶의 만족을 측정하여 분석하였다. 온라인 코칭프로그램의 효과성을 검증하기 위하여 프로그램 실시 사전, 사후의 시점과 집단 간의 상호작용효과를 분석한 결과, 자기조절과 삶의 만족을 제외한 체중, 식습관, 식이행동, 식이효능감에서 시점과 집단 간에 유의미한 결과가 나타나 프로그램 효과가 검증되었다. 즉, 통제집단에 비하여 실험집단에서 체중, 식습관, 식이행동, 식이효능감에서 유의미한 변화가 있었다. 이상의 연구 결과를 중심으로 본 연구의 의의 및 실무적 시사점, 제한점, 그리고 후속 연구를 위한 제언 등에 관해 논의하였다.

• Biomolecules & Therapeutics
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• 제2권2호
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• pp.190-205
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• 1994

This study was conducted to investigate the repeated dose toxicity of DA-125, a new anthracycline antitumor antibiotic, in rats. Before the 13-week main study, a 4-week dose-range finding (DRF) study was carried out. The administration of DA-125 intravenously at dosage levels of 0, 0.125, 0.5, 2.0, and 8.0 mg/kg/day to rats for 4 weeks resulted in premature deaths of all animals in the 8.0 mg/kg/day group and in the deaths of 4 males and 4 females at 2.0 mg/kg/day. Body weights were markedly reduced in the 8.0 mg/kg/day group and showed dose-related decreases in all treatment groups when compared with the control group. Reductions in weight gain were slight and not significantly different at 0.125 mg/kg/day but animals receiving 0.5 mg/kg/day showed more marked decreases in gain in a clear dose-related manner Based On the results of the above DRF study, a 13-week repeated dose intravenous toxicity study in rats with DA-125 was performed at a dose level of 0, 0.012, 0.08 and 0.3 mg/kg/day. No treatment related effects were noted in behavior or body weight in all treatment groups. One male at the highest dose level died on study day 26, but the death could not be related to test article toxicity. Swelling and scabbing of the ears was present in all of the groups, including the control group. There were no treatment related changes in the hematological, biochemical or urinalysis values in all treatment groups. Thymus weights were significantly reduced ill males receiving 0.3 mg/kg/day and they were sligltly, and not significantly, reduced in females of the same group. While there were no associated histological changes. Treatment related necrosis was found in the tail vein (injection site) at 0.08 and 0.3 mg/kg/day. On the basis of these results, the no observed effect level (NOEL) was 0.012 mg/kg/day and the maximum tolerated dose (MTD) was estimated to be more than 0.3 mg/kg/day under the conditions tested.