• Food Science and Biotechnology
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• v.18 no.1
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• pp.31-35
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• 2009

Total folate content was determined by microbiological assay using Lactobacillus casei spp. rhamnosis (ATCC 7469) with a 96-well microplate technique. Using roasted peanut and red kidney beans as representative legume samples, response surface methodology (RSM) was supplied to optimize the trienzyme procedures for the determination of folate in legumes. After response surface regression (RSREG), the second-order polynomial equation was fitted to the experimental data. Ridge analysis showed that the optimal digestion times were <2 hr for $Pronase^{(R)}$ and $\alpha$-amylase, and <5 hr for conjugase to obtain maximal folate values for legume samples. This study confirms that established digestion times for cereal products (AOAC Method 2004.05) of 3 for protease and 2 hr for $\alpha$-amylase are applicable to legumes. Conjugase treatment can be reduced to 5 from 16 hr and the conjugase level to 5 from 20 mg per sample, providing significant cost saving.

• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.173-179
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• 2019

The aim of this study is to investigate the biological activities of Hericium erinaceus. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of H. erinaceus extract was higher than positive control. The inhibitory activities of xanthin oxidase, ${\alpha}$-glucosidase, and hyaluronidase was measured as functional food activity, and inhibitory activities on collagenase, tyrosinase, and astringent effect as beauty food activity in water and ethanol extracts from H. erinaceus. In functional food activity, xanthin oxidase inhibitory activities at $50-200{\mu}g/mL$ phenolic concentration in ethanol extracts from H. erinaceus showed inhibitory activity in dose dependent manner. ${\alpha}$-Glucosidase inhibitory activities at $50{\mu}g/mL$ phenolic concentration showed high activity of higher than 80%. Inhibitory activities on hyaluronidase as anti-inflammation factor showed inhibition effect in dose dependent manner both in water and ethanol extracts. In beauty food activity, Inhibitory activities on collagenase at $200{\mu}g/mL$ phenolic concentration in water and ethanol extracts showed high activity to 65.09 and 58.38% dose dependently. Tyrosinase inhibitory activity in water extract showed 9.4-58.24%. Astringent activity as pore shrink effect in ethanol extracts also showed a very high activity of 18.94-100%. Antimicrobial activity on pathogenic bacteria was highly effective on Staphylococcus aureus, Salmonella enteritidis, Vibrio parahaemolyticus and Escherichia coli at 2.5 mg/mL or above. Therefore, the extracts from H. erinaceus can be used as a functional food and beauty food resources and natural antimicrobial agent on pathogenic bacteria in food.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.7
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• pp.2886-2894
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• 2012

This study is intended to research female university students' concern with beauty care, their skin condition, knowledge and treatment comparing the majors with the non-majors in Seoul. This comparative study between them focused on their educational and behavioral differences in beauty care in the same living space, and the results were as follows: The majors' average point on the concern with skin health care was higher than the non-majors(p<.01). There were more combination skin type in the major group, and more dry skin type in the non-major group. Also, there were meaningful differences among the two groups in frequency of double cleansing after makeup, exfoliating, massaging or applying mask packs, using sunscreen(p<.01, p<.001, p<.01, p<.01). Further, intake rates of instant food and moisture were meaningfully different between them(p<.001, p<.05). In addition, the majors more tended to purchase cosmetics efficiently through lots of channels, and showed more active attitude to buy high-price cosmetics with outstanding properties although they have financial burden(p<.001). It was hoped that the results of this study would be used in skin care industry and education area in the future.

• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.207-215
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• 2012

Phenolic compounds of $17.9{\pm}1.0mg/g$ were extracted from Ulmus pumila with 70% ethanol. The elastase inhibitory activity related with forming wrinkle was shown an excellent wrinkle improvement effect in 70% ethanol extracts as 55.5-69.5% at phenolic concentration of $50-200{\mu}g/mL$. The tyrosinase inhibitory activity related with skin-whitening was 24% in 70% ethanol extracts at phenolic concentration of $200{\mu}g/mL$. The astringent activity of 70% ethanol extracts was shown activity of 71% at phenolic concentration of $200{\mu}g/mL$ therefore it is judged that there is a high effect on pores reduction of the skin. The hyaluronidase inhibitory activity of U. pumila extracts was confirmed anti-inflammation effect of 80% at phenolic concentration of $50{\mu}g/mL$. Antimicrobial activity of U. pumila water extracts was shown each 8.7, 10.0, 11.1 and 11.8 mm clear zones on Propionebacterium acnes at phenolic concentration of $50-200{\mu}g/mL$. The stability of the multi-functional cosmetic (lotion) added U. pumila extracts was very stable for 28 days without changing of pH and viscosity also it's stable on temperature and sun lights. As the concentration of extracts was increased, the color of lotion was getting dark, but the sensory evaluation was high at score of 8.5.

• The Korean Journal of Community Living Science
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• v.25 no.2
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• pp.193-205
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• 2014

This study investigates soldiers' and civilians' interest in and behaviors toward cosmetics and skin beauty. Responses to a self-administered questionnaire were collected from 150 soldiers and 156 civilians residing in Chung-chung Province, Korea. The mean age of the respondents was $21.9{\pm}1.8$. More than 90% used skin toner and lotion products, and more than 50% used sunblocks and foam cleansers. Soldiers were more likely to use essence (p<.001) and after-shave (p<.01) products than civilians. In addition, soldiers were more likely to use carrier cosmetics than their counterparts (p<.001~p<.05). Soldiers were more likely to be concerned about their skin (p<.01) and unsatisfactory environments for skin care (p<.001), more likely to engage in skin care behaviors (p<.01), and less likely to have skin knowledge than civilians (p<.001). There was a negative relationship between skin knowledge and skin care behaviors (p<.01). The level of interest in appearance was correlated with that in skin (p<.001). The number of skin related concerns was positively correlated with unsatisfactory environments for skin care (p<.001). Concerns over appearance (p<.001), skin (p<.001), and unsatisfactory environments for skin care (p<.05) were positively correlated with skin care behaviors. The results suggest the necessity of providing a diverse and differentiated range of cosmetics to meet the needs of soldiers and men, particularly the former, according to the differences in their environments.

• KSBB Journal
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• v.32 no.2
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• pp.117-123
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• 2017

Maple tree is a useful medical plant for obtaining bioactive materials such as pharmaceutics, cosmetics, food additive, etc., and there are 16 species of native maple trees in Korea. In this study, we evaluated the antioxidant activity of sap and crude extracts of Acer mono Max, a representative maple species. The crude extracts were obtained by solvent extraction (water, ethanol, and ethyl acetate) from its branches (bark and xylem). The phenolic contents and radical scavenging capacities of the extracts and the sap were evaluated in terms of half maximal effective concentration ($EC_{50}$) and kinetics by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanol extracts showed the highest extraction yield, phenolic contents and antioxidant activity, and bark extracts showed better antioxidant activity than xylem extracts. The antioxidant activity of the sap was very low, but the $EC_{50}$ of ethanol and ethyl acetate extracts ranged from 68 to $79{\mu}g/mL$, similar to that ($60{\mu}g/mL$) of the control, butylated hydroxytoluene (BHT). The DPPH radical scavenging rate ($220{\sim}760{\mu}M/min$) and the second-order reaction rate constant ($6.48{\sim}7.04L/g{\cdot}min$) of these extracts were better than those of BHT ($55{\sim}370{\mu}M/min$ and $3.60L/g{\cdot}min$). These results suggest that A. mono Max. is one of the useful bioresources for obtaining antioxidant biologically active substances, and it is possible to obtain physiologically active substances from by-product of its pruning while minimizing the effect on the growth of the tree.

• Journal of the Korean Society of Fashion and Beauty
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• v.3 no.3 s.3
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• pp.41-47
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• 2005

This study was conducted with 60 adult males who visited the Atomi Cooperative Clinic(Atomi Dermatology/Pediatrics/Oriental Clinic) in Seoul between May 2003 and December 2004; they were divided into three groups, each of which consisted of 20 persons, according to the level of erythma and then each group was subdivided to consist of ten according to whether they received skin treatment and care. The standard care provided to two groups involved topical steroids and oriental medicines prescribed by a dermatologist and a oriental doctor, respectively. 5th-grade topical steroid ointment was applied to the face and 3rd-grade to the limbs; a oriental medicine was administered in a lukewarm state half an hour after meals three times a day. To determine how special and systematic skin treatment and care was helpful in treating atopy, a skin treatment system was applied to the experimental group while the control group was provided with standard care alone. By using Mexameter(MX18) manufactured by ck-mpa as a measuring tool, the inflammation level was observed at the right antecubital space during each visit to the clinic. In view of the re suits, introduction of the systemic skin care for A. D to legitimate treatment provided by a medical institution is expected to be an appropriate supplementary treatment for adult patients who suffer from frequent recurrence of atopic dermatitis.

• Biomolecules & Therapeutics
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• v.24 no.5
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• pp.552-558
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• 2016

Severe complications associated with EV71 infections are a common cause of neonatal death. Lack of effective therapeutic agents for these infections underlines the importance of research for the development of new antiviral compounds. In the present study, the anti-EV71 activity of norwogonin, oroxylin A, and mosloflavone from Scutellaria baicalensis Georgi was evaluated using a cytopathic effect (CPE) reduction method, which demonstrated that all three compounds possessed strong anti-EV71 activity and decreased the formation of visible CPEs. Norwogonin, oroxylin A, and mosloflavone also inhibited virus replication during the initial stage of virus infection, and they inhibited viral VP2 protein expression, thereby inhibiting viral capsid protein synthesis. However, ribavirin has a relatively weaker efficacy compared to the other drugs. Therefore, these findings provide important information that will aid in the utilization of norwogonin, oroxylin A, and mosloflavone for EV71 treatment.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.1
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• pp.250-253
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• 2004

The effects of crude extracts from medicinal plants on biological activity were investigated. The crude ethanol extract of H. paucistipula inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (2 mm inhibition zone at 150 ㎍/disc) and the dermatophyte Trichophyton mentagrophytes ATCC 28185, (7 mm inhibition zone at 150 ㎍/disc), and toxic to P388 murine leukaemia cells ATCC CCL 46 P388D1, (IC/sub 50/ 2.48 ㎍/㎖ at 75 ㎍/disc). This crude ethanol extract of H. paucistipula is the strongest antimicrobial and cytotoxic activities against P388 murine leukaemia cells ATCC CCL 46 P388D1.

• Journal of Fashion Business
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• v.7 no.6
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• pp.10-20
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• 2003

The object of this research is to develop the designs with aesthetics and function for making the uniforms of specialty restaurants of Korean dishes in pursuit of the image of excellent dignity and its result is as follows: As for designs, this research chose the traditional image as the basic concept and made visual Korean lines, colors, and patterns. As for lines, it made visual the curve of the eaves, the straight line of polls, and the fret of windows and doors represented in architecture and applied them, as for color tones, it chose traditional 'Obangsaek', five direction colors. As for the patterns, it symbolized 4 trigrams( Geon, Gon, Gam, and Yi), the cloud pattern, also it tried to get the formative beauty from traditional patchwork wrapping cloth and windows and doors. The expectant effects on the design of Uniform are as follows: First, it offered basic clothes for male and female employees working in the hall and suggested two kinds of skirt and pants for the latter. It tried to find out both the function of pants and the female beauty of skirts by wrapping on pants to eliminate the feeling of rejection towards the style of them, the use of which have been recognized for man only in spite of many merits of them. Second, it sought for the characteristics of shape on collar, breast-tie, and fold etc. of Korean clothes and designed clothes according to each employee's role and finally emphasized their traditional aesthetics.