• 대한영양사협회학술지
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• 제2권2호
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• pp.141-157
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• 1996

This study was carried out to obtain the information concerning food preferences of 588 men working at industry in Ulsan area. The subjects of this study were composed of 245 clerical workers and 343 physical workers. The results of this study can be summarized as follows: 1. Average age of this subjects was 36.1 years(ranging from 21 to 57), in the educational background, most of subjects were high school graduates. The mean height and weight were l71.6cm and 67.5kg. 2. In the preference of food 1) The preference for staple foods, well-milled cooked rice was preferred the most by the subjects. Kalkooksoo(hot noodle), cooked rice covered with raw fish pieces, bread with red bean were preferred the most by the workers. 2) The acceptance for side-dishes were high in sirak(woogyogi) soup, bach tang, soybean paste soup, broiled fish, wild plant namul, small radish kimchi and crab steaming products by clerial and physical workers all together. 3) The preferences for fruits and beverages were high in water-melon, pear, fruit juice and Korean traditional beverage.

• 한국의류학회지
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• 제8권3호
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• pp.1-7
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• 1984

Traditionally dyeing fabrics with pigment extracted from carthamus flower was a popular method of obtaining red color in Korea. Such a method existed in several countries throughout the world before the synthetic dyestuff was developed. However, the traditional procedures of using natural products in obtaining colored materials are completely forgotten in modern Korea. The details of dyeing procedures fare not well documented in literature, either. In this study the method of extracting and dyeing with carthamus flower is reconstructed from Korean literature and actually carried out in laboratorf. The reconstructed method is compared with those of Japan, China, France, and Egypt, and the scientific basis of such an ancient tradition is discussed. Carthamus contains two kinds of coloring components : yellow carthamin and red carthamone. Water-soluble carthamin is first extracted by repeated washing and is usually used for the initial soaking procedure. Then carthamone is extracted in alkali solution by adding ashes of dried plants such as carthamus stalks and bean hull. Finally the solution of carthamone is made acidic by adding schizandra juice for dyeing on fabrics.

• 농약과학회지
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• 제14권1호
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• pp.21-29
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• 2010

농산물 중 bifenazate의 잔류 실태를 조사하기 위하여 HPLC를 사용한 분석법을 개선하였고, LC/MS 분석법을 확립하였다. 개선된 분석법에 대한 직선성과 재현성에 대한 검증을 수행한 결과 bifenazate 0.05~2.5 mg/kg 범위에서 상관계수는 1.0이었고, 정량한계(LOQ)는 0.01 mg/kg이었다. 회수율은 현미(곡류), 강낭콩(콩류), 오렌지(과실류), 들깻잎(채소류), 표고버섯(버섯류)에 대해 0.1 mg/kg 수준에서 82.7~104.1%였고, 0.5 mg/kg에서는 73.1~104.3%였다. 또한 회수율에 대한 상대표준편차(n=3)는 0.2~9.7%였다. 전국 22개 지역에서 수거한 농산물 16품목(쌀, 조, 메밀, 강낭콩, 땅콩, 참깨, 오렌지, 자몽, 키위, 시금치, 들깻잎, 부추, 마늘쫑, 마늘, 생강, 표고버섯) 304건을 대상으로 bifenazate의 잔류량을 조사한 결과 모든 농산물에서 검출되지 않았다.

• 미생물학회지
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• 제54권2호
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• pp.126-135
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• 2018

여러 종류의 mannanase를 생산하는 Cellulosimicrobium sp. YB-43으로부터 mannanase B를 암호하는 manB 유전자와 효소의 특성이 보고된 바 있다. Mannanase C (ManC)로 명명한 효소의 유전자가 manB 유전자의 하류에 위치한 것으로 예상되어 이를 중합효소 연쇄반응으로 클로닝하여 manC 유전자의 염기서열을 결정하였다. ManC는 448 아미노산 잔기로 구성된 것으로 확인되었으며 glycosyl hydrolase family 5에 속하는 mannanase와 상동성이 높은 활성영역과 탄수화물 결합영역(CBM2)이 존재하였다. ManC의 활성영역은 Streptomyces sp. SirexAA-E (55.8%; 4FK9_A) 및 S. thermoluteus (57.6%; BAM62868)의 mannanase와 아미노산 배열의 상동성이 55% 이상으로 가장 높았다. Signal peptide 영역이 제거되고 카르복실 말단에 hexahistidine이 연결되도록 제조한 His-tagged ManC (HtManC)의 유전자를 재조합 대장균에서 발현하여 균체 파쇄액으로부터 HtManC를 정제하였다. HtManC은 $65^{\circ}C$와 pH 7.5에서 최대 활성을 보였으며 pH 7.5~10범위에서 활성에 큰 변화가 없었다. HtManC는 locust bean gum (LBG)과 konjac에 대한 분해 활성이 guar gum과 ivory nut mannan (ivory nut)에 비해 높았다. 최적 반응조건에서 LBG를 기질로 하여 반응 동력학적 계수를 측정한 결과 Vmax와 Km이 68 U/mg과 0.45 mg/ml로 나타났다. HtManC에 의한 만노올리고당(MOS)과 mannan의 분해산물을 TLC로 관찰한 결과 mannobiose 보다 중합도가 큰MOS로부터 mannobiose와 mannotriose가 주된 분해산물로 생성되었다. 또한 LBG, konjac과 ivory nut의 분해산물로 mannobiose와 소량이 mannose가 공통적으로 관찰되었다.

• 대한지역사회영양학회지
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• 제16권4호
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• pp.411-425
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• 2011

This study was conducted to examine differences in nutrient intakes by the compliance with the Dietary Action Guide for Korean Children. The subjects included 343 elementary school students in Gwangju city. Compliance with the Dietary Action Guide for Children showed that 62.4% of subjects had breakfast everyday; 44.3% of subjects ate vegetables/fruits/milk and dairy products daily; 26.8% of subjects ate a variety of lean meats/fish/eggs/bean products daily; 32.9% of subjects enjoyed outdoor activity everyday and ate according to their energy needs; 40.2% of subjects chose healthy and nutritious foods for snack; and 15.5% of subjects avoided food waste. Intakes of most of nutrients including energy were significantly higher (p < 0.05) in those who complied with the Dietary Action Guide well. Also nutrient intakes had the positive correlation with the scores of Dietary Action Guide (p < 0.05). From these results, nutrient intakes of children were significantly influenced by dietary factors suggested in the Dietary Action Guide for Children. In addition, the results also confirmed that good food behaviors were indispensible to maintaining a proper nutritional status. Based on these results, good food behaviors and practice were critical to secure good health and proper nutritional status for children, and that nutrition education should be strengthened in school as well as at home.

• 약학회지
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• 제4권1호
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• pp.50-52
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• 1959

In order to know contents of vitamin B$_{12}$ in the fermented foods in Korea, the contents of this vitamin are studied on the following foods; kimchies (pickled vegetables) .................. 39 chukkals (fermented and salted sea foods) ...... 8 fermented soy-bean products .................... 5 the kimchies and fermented soybean products studied in this paper, are from individual homes and chukkals are from markets. The content of vitamin B$_{12}$ is estimated by the microbiological assay method using lactobacillus leichmannii ATCC 7830. Details for assay are indicated in the original part of this paper. The pseudo-vitamin B$_{12}$ substances as thymidine which is active to the growth of lactobacillus leichmannii, are eliminated by the alkali treatment method of sample solution. According to the results as indicated in the table, the following conclusions are summerized; 1. Vitamin B$_{12}$ contents of kimchies for winter season are 1.03 to 1.52 mcg% in average. The hobakk-kimchi which contain the highest content of vitamin B$_{12}$ is not popular one among Korean. 2. Chukkals contain much higher content of vitamin B$_{12}$ varying from 0.91 to 11.10mcg%. 3. The soybean fermented foods, as kanjang, denjang, kochojang, contain lower content of vitamine B$_{12}$ varying from 0.08 to 0.52 mcg% containing higher content of pseudo-vitamin. 4. Based on daily consumption of kimchi in winter season by Chai, the consumption of this vitamin through kimchi is about 3 mcgs daily per capita. This will be a quite source of this vitamin for Korean as vitamin C. as vitamin C.

• 한국식품조리과학회지
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• 제14권1호
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• pp.33-43
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• 1998

This study was peformed by questionnaire to investigate the recognition, preference and intake of calcium and related food behaviors of middle-aged people (males 600, females 550) in Daegu area. The daily intake was determined by 24-hr dietary recall method. The results were summarized as follows: The meal skipping, diet and nutrition consideration ratio of the subjects were 40.6, 11.1, 34.6% respectively. The recognition scores of calcium of the subjects were 14.3 for male and 15.1 for female, respectively. Also the preference scores of calcium source foods of the subjects were 3.6 for male and 3.5 for female. They preferred laver, cabbage, bean curd and lettuce, in that order, but they disliked cheese and ice cream. The variety of calcium source foods was remarkably low. Milk products were the lowest preferred and eaten calcium source foods. A day's calcium intake was 68.4% (478.6 mg) of the RDA for Koreans. There were significant positive correlations among the recognition, preference and intake scores of calcium.

• Journal of Microbiology and Biotechnology
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• 제24권2호
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• pp.177-187
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• 2014

Microbial phytases are enzymes with biotechnological interest for the feed industry. In this article, the effect of spray-drying conditions on the stability and activity of extracellular phytase produced by R. microsporus var. microsporus biofilm is described. The phytase was spray-dried in the presence of starch, corn meal (> $150{\mu}m$), soy bean meal (SB), corn meal (< $150{\mu}m$) (CM), and maltodextrin as drying adjuvants. The residual enzyme activity after drying ranged from 10.7% to 60.4%, with SB and CM standing out as stabilizing agents. Water concentration and residual enzyme activity were determined in obtained powders as a function of the drying condition. When exposed to different pH values, the SB and CM products were stable, with residual activity above 50% in the pH range from 4.5 to 8.5 for 60 min. The use of CM as drying adjuvant promoted the best retention of enzymatic activity compared with SB. Spray drying of the R. microsporus var. microsporus phytase using different drying adjuvants showed interesting results, being quite feasible with regards their biotechnological applications, especially for poultry diets.

• Journal of Ginseng Research
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• 제37권1호
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• pp.74-79
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• 2013

In continuation of our research to find biological components from Panax stipuleanatus, four oleanane-type triterpenes (12 to 15) were isolated successively. Fifteen oleanane-type saponins (1 to 15) were evaluated for nuclear factor (NF)-${\kappa}B$ activity using a luciferase reporter gene assay in HepG2 cells. Compounds 6 to 11 inhibited NF-${\kappa}B$, with $IC_{50}$ values between 3.1 to 18.9 ${\mu}M$. The effects on inducible nitric oxide synthase and cyclooxygenase-2 by compounds 8, 10, and 11 were also examined using reverse transcription-polymerase chain reaction. Three compounds (8, 10, and 11) inhibited NF-${\kappa}B$ activity by reducing the concentration of inflammatory factors in HepG2 cells.

• 한국식품조리과학회지
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• 제28권6호
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• pp.741-751
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• 2012

This study was conducted to understand sensory characteristics of commercial functional beverages, to analyze and the drivers of liking and disliking of it by Korean consumers. Descriptive analysis and consumer taste testing were conducted with ten commercial products of functional beverages. Samples were consisted of good for beauty, relieving hangovers, and health tonics. For the descriptive analysis, 45 attributes were developed by ten panelists and it shows differences among the all samples. For the consumer testing, 81 panels evaluated the overall liking, acceptance of appearance, odor, flavor, and texture of 10 samples. As a result, attributes of brightness, yellow color, Nurungji flavor, roasted bean power flavor, and milky texture of functional beverages were positive drivers of liking, but attributes of astringent texture, bitter taste, and viscosity were negative drivers of liking on the commercial functional beverages.