Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-κB

  • Liang, Chun (College of Pharmacy, Chungnam National University) ;
  • Ding, Yan (Institute of Chemistry and Applications of Plant Resources, Dalian Polytechnic University) ;
  • Song, Seok Bean (College of Pharmacy, Chungnam National University) ;
  • Kim, Jeong Ah (College of Pharmacy, Kyungpook National University) ;
  • Nguyen, Manh Cuong (Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology) ;
  • Ma, Jin Yeul (Herbal Medicine Improvement Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Young Ho (College of Pharmacy, Chungnam National University)
  • Received : 2012.05.21
  • Accepted : 2012.09.07
  • Published : 2013.01.15
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Abstract

In continuation of our research to find biological components from Panax stipuleanatus, four oleanane-type triterpenes (12 to 15) were isolated successively. Fifteen oleanane-type saponins (1 to 15) were evaluated for nuclear factor (NF)-${\kappa}B$ activity using a luciferase reporter gene assay in HepG2 cells. Compounds 6 to 11 inhibited NF-${\kappa}B$, with $IC_{50}$ values between 3.1 to 18.9 ${\mu}M$. The effects on inducible nitric oxide synthase and cyclooxygenase-2 by compounds 8, 10, and 11 were also examined using reverse transcription-polymerase chain reaction. Three compounds (8, 10, and 11) inhibited NF-${\kappa}B$ activity by reducing the concentration of inflammatory factors in HepG2 cells.

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References

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